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The map label for this gene is dctB [H]

Identifier: 183221501

GI number: 183221501

Start: 2212088

End: 2213362

Strand: Reverse

Name: dctB [H]

Synonym: LEPBI_I2119

Alternate gene names: 183221501

Gene position: 2213362-2212088 (Counterclockwise)

Preceding gene: 183221502

Following gene: 183221500

Centisome position: 61.49

GC content: 37.1

Gene sequence:

>1275_bases
ATGTTACAAGGAACATTATTATCAGAGATCATTGAAGCTGTTTCCAATACCTATGGAAATGAATTTTTAAATCGACTCAC
CGAAAAACTATGTACGGTAATCGGTGCTGATTATACATTTATTGGACTTTTTGATAAAAAAAATTATGAGTCGAGGACTG
TCTCCCTTGTGGCAAAGGGCCAAGTCGTAGATAATTTTAATTACAGTTTGGAAGGGACTCCTTGTGCCAATGTCTTTGAG
AATAAAATTTGTTATTATCCAACAGAGGTACAAAAACTTTTTCCAAACGACCATCTGTTAGTGGATATGAAAATTGATGG
GTACATAGGGACTCCTCTCATCAATTCGAAACAAGAAATTTTGGGTTTGATCGTTGGTTTGTTTGAACAAAAGATTCCCA
AAAAAGAGGAAACTTTGACATTGTTTCAAATTTTTTCGGGTAGGATTGCTGCGGAGTTAGAGCGACTTGATTACGAAAGA
AAACTAGAACAATACAACGAAGAGTTGGAAGTTTTGGTTAAGGAACGAACATTTAAGTTAGAACAAACATTAGAGGAACT
AAAGGAACGCCAAGACCAACTCGTGGAAGCAGAAAAGTTGGCAAGTTTAGGACTTTTATCCGCAGGCATTGCTCATGAGA
TCAATAACCCATTAAACTTCATTACGGGTGGTTATTATGGCATCAAAGATTTCATTCAATCGACAGGGATCCAATCGGAA
AAAACAGAAATGTATTTAGATGCCATCAAAGTGGGAGTGGAACGGACAACAAAAATTGTCAAGGGCCTAAACCAATATAC
AAGGACTGGTGATTCCTTTGATGAAAAAATTGATATTGAAGAAATGTTAGAAAACTGTTTGGTGATCTTATTTCATACTT
TCCGAGATAAAATTTCTGTCGAAAAAGAATTTGATTCTGAACCAGTTTTCATCCAAGGGAACTCGGGTAAAATCCACCAG
GTTTTTCTGAACCTCCTCACCAATGCGATTCAGTCTATGGAAAACAAAAGAGGAAAACTTTTGATTTCTGCAAAGTCAGA
AGGGGATTTTGTTTTGGTCACGATCCAAGATACAGGAACTGGGATCCGAAAAGAACACCTAAACCAAATTCAAATTCCTT
TTTTTACCACAAAAGAACCAGGCAAAGGTGTGGGCCTTGGTCTATCCATTGCTTATAAAATTTTGAAAGAACATTCGGCA
TCAATTGATGTGGAATCGGAATGGGGGGAGGGGACAAAATTTACGATCCGTCTTCCCAAATGGAATGAAGGTTAA

Upstream 100 bases:

>100_bases
GAAACTTCCTTTGGTTAGTGAACCAAAGGAAACAGAACCGAAGGCAAATCAATAAAAGGAGATTATCTTTGGTACTAGTT
TTCTGAACCGCTAAAACTTT

Downstream 100 bases:

>100_bases
ATTTTGAAACCAAAACTACTTTATGTGGATGATGAAGTCATGAATTTACATCTATTCCGAGAAATGTTTCGAAAGGATTT
TGATGTTTTGATTGCAAGTT

Product: putative two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MLQGTLLSEIIEAVSNTYGNEFLNRLTEKLCTVIGADYTFIGLFDKKNYESRTVSLVAKGQVVDNFNYSLEGTPCANVFE
NKICYYPTEVQKLFPNDHLLVDMKIDGYIGTPLINSKQEILGLIVGLFEQKIPKKEETLTLFQIFSGRIAAELERLDYER
KLEQYNEELEVLVKERTFKLEQTLEELKERQDQLVEAEKLASLGLLSAGIAHEINNPLNFITGGYYGIKDFIQSTGIQSE
KTEMYLDAIKVGVERTTKIVKGLNQYTRTGDSFDEKIDIEEMLENCLVILFHTFRDKISVEKEFDSEPVFIQGNSGKIHQ
VFLNLLTNAIQSMENKRGKLLISAKSEGDFVLVTIQDTGTGIRKEHLNQIQIPFFTTKEPGKGVGLGLSIAYKILKEHSA
SIDVESEWGEGTKFTIRLPKWNEG

Sequences:

>Translated_424_residues
MLQGTLLSEIIEAVSNTYGNEFLNRLTEKLCTVIGADYTFIGLFDKKNYESRTVSLVAKGQVVDNFNYSLEGTPCANVFE
NKICYYPTEVQKLFPNDHLLVDMKIDGYIGTPLINSKQEILGLIVGLFEQKIPKKEETLTLFQIFSGRIAAELERLDYER
KLEQYNEELEVLVKERTFKLEQTLEELKERQDQLVEAEKLASLGLLSAGIAHEINNPLNFITGGYYGIKDFIQSTGIQSE
KTEMYLDAIKVGVERTTKIVKGLNQYTRTGDSFDEKIDIEEMLENCLVILFHTFRDKISVEKEFDSEPVFIQGNSGKIHQ
VFLNLLTNAIQSMENKRGKLLISAKSEGDFVLVTIQDTGTGIRKEHLNQIQIPFFTTKEPGKGVGLGLSIAYKILKEHSA
SIDVESEWGEGTKFTIRLPKWNEG
>Mature_424_residues
MLQGTLLSEIIEAVSNTYGNEFLNRLTEKLCTVIGADYTFIGLFDKKNYESRTVSLVAKGQVVDNFNYSLEGTPCANVFE
NKICYYPTEVQKLFPNDHLLVDMKIDGYIGTPLINSKQEILGLIVGLFEQKIPKKEETLTLFQIFSGRIAAELERLDYER
KLEQYNEELEVLVKERTFKLEQTLEELKERQDQLVEAEKLASLGLLSAGIAHEINNPLNFITGGYYGIKDFIQSTGIQSE
KTEMYLDAIKVGVERTTKIVKGLNQYTRTGDSFDEKIDIEEMLENCLVILFHTFRDKISVEKEFDSEPVFIQGNSGKIHQ
VFLNLLTNAIQSMENKRGKLLISAKSEGDFVLVTIQDTGTGIRKEHLNQIQIPFFTTKEPGKGVGLGLSIAYKILKEHSA
SIDVESEWGEGTKFTIRLPKWNEG

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=33.6134453781513, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790436, Length=231, Percent_Identity=29.004329004329, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=24.4094488188976, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI87081816, Length=266, Percent_Identity=25.5639097744361, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=25.9414225941423, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI48994928, Length=256, Percent_Identity=24.609375, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48043; Mature: 48043

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQGTLLSEIIEAVSNTYGNEFLNRLTEKLCTVIGADYTFIGLFDKKNYESRTVSLVAKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEEC
QVVDNFNYSLEGTPCANVFENKICYYPTEVQKLFPNDHLLVDMKIDGYIGTPLINSKQEI
CEEECCCCEECCCCCHHHHCCCCEECCHHHHHHCCCCCEEEEEEECCEECCCCCCCHHHH
LGLIVGLFEQKIPKKEETLTLFQIFSGRIAAELERLDYERKLEQYNEELEVLVKERTFKL
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQTLEELKERQDQLVEAEKLASLGLLSAGIAHEINNPLNFITGGYYGIKDFIQSTGIQSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCH
KTEMYLDAIKVGVERTTKIVKGLNQYTRTGDSFDEKIDIEEMLENCLVILFHTFRDKISV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EKEFDSEPVFIQGNSGKIHQVFLNLLTNAIQSMENKRGKLLISAKSEGDFVLVTIQDTGT
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCC
GIRKEHLNQIQIPFFTTKEPGKGVGLGLSIAYKILKEHSASIDVESEWGEGTKFTIRLPK
CHHHHCCCEEECCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCC
WNEG
CCCC
>Mature Secondary Structure
MLQGTLLSEIIEAVSNTYGNEFLNRLTEKLCTVIGADYTFIGLFDKKNYESRTVSLVAKG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEEEC
QVVDNFNYSLEGTPCANVFENKICYYPTEVQKLFPNDHLLVDMKIDGYIGTPLINSKQEI
CEEECCCCEECCCCCHHHHCCCCEECCHHHHHHCCCCCEEEEEEECCEECCCCCCCHHHH
LGLIVGLFEQKIPKKEETLTLFQIFSGRIAAELERLDYERKLEQYNEELEVLVKERTFKL
HHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQTLEELKERQDQLVEAEKLASLGLLSAGIAHEINNPLNFITGGYYGIKDFIQSTGIQSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCH
KTEMYLDAIKVGVERTTKIVKGLNQYTRTGDSFDEKIDIEEMLENCLVILFHTFRDKISV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EKEFDSEPVFIQGNSGKIHQVFLNLLTNAIQSMENKRGKLLISAKSEGDFVLVTIQDTGT
HHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCC
GIRKEHLNQIQIPFFTTKEPGKGVGLGLSIAYKILKEHSASIDVESEWGEGTKFTIRLPK
CHHHHCCCEEECCEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCEEEEECCC
WNEG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]