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The map label for this gene is nuoB

Identifier: 183220687

GI number: 183220687

Start: 1351689

End: 1352249

Strand: Direct

Name: nuoB

Synonym: LEPBI_I1297

Alternate gene names: 183220687

Gene position: 1351689-1352249 (Clockwise)

Preceding gene: 183220686

Following gene: 183220688

Centisome position: 37.55

GC content: 45.81

Gene sequence:

>561_bases
ATGGGATTAACAGAAACACTATCCAAACCAGGCGAGATGTTTGGTGATATGTTCCAAGTTGCCACACTCGACAATGTCGT
GCAGTGGGGGCAAAGTTTTTCTTTATGGCCTTATCCTTTTGCCACAGCTTGTTGTGGGATTGAATACATGAGTACGGCTT
GTGCCGATTATGATATTGCTCGGTTTGGAGCCGAAAGACCATCTTTTTCTCCACGCCAGGCCGATATGATCTTAGTGCTT
GGGACCATCACTTATAAGATGGCTCCCGTATTACGCCAGATATACGACCAACTTGCAGAACCCAAATTTGTGATCTCTGT
GGGAGCCTGTGCCTCATCCGGTGGAATGTTTCACACCTATGGTGTGTTACAAGGTGTTGATCGAATATTACCCGTGGATG
TGTATGTTCCAGGTTGCCCTCCAAGACCAGAAGCCATTCTTGATGCACTCGTAAAGCTACAAAAAAAAGTACAAAGCCAA
GGATTGGAAGCACGTCGCCAAGAAGTCATGAGAAAGATCGAAGAGATCAATGAACGTAACAAACCCCTCGTAGTGGCATG
A

Upstream 100 bases:

>100_bases
AAGAAGCGGGTTTGGGTACGTTTTTTCTTTTCGAAATGTTTTTCTTTTTACTCATCTTAGTTGTGGGTCTATATTATATC
TGGAAAAAAGGAGCACTGGA

Downstream 100 bases:

>100_bases
AAGAAAAACTTACCGAATTCATCACAACCCGATTTGTGGATTGTTTACTCCCGCAAAGGGACATAAACACGAATCTTTTG
TACTTCAGCATCCAAAAAGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MGLTETLSKPGEMFGDMFQVATLDNVVQWGQSFSLWPYPFATACCGIEYMSTACADYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQLAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALVKLQKKVQSQ
GLEARRQEVMRKIEEINERNKPLVVA

Sequences:

>Translated_186_residues
MGLTETLSKPGEMFGDMFQVATLDNVVQWGQSFSLWPYPFATACCGIEYMSTACADYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQLAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALVKLQKKVQSQ
GLEARRQEVMRKIEEINERNKPLVVA
>Mature_185_residues
GLTETLSKPGEMFGDMFQVATLDNVVQWGQSFSLWPYPFATACCGIEYMSTACADYDIARFGAERPSFSPRQADMILVLG
TITYKMAPVLRQIYDQLAEPKFVISVGACASSGGMFHTYGVLQGVDRILPVDVYVPGCPPRPEAILDALVKLQKKVQSQG
LEARRQEVMRKIEEINERNKPLVVA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=144, Percent_Identity=49.3055555555556, Blast_Score=162, Evalue=1e-40,
Organism=Escherichia coli, GI1788624, Length=138, Percent_Identity=54.3478260869565, Blast_Score=173, Evalue=5e-45,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=44.7154471544715, Blast_Score=93, Evalue=9e-21,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=41.3223140495868, Blast_Score=84, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=144, Percent_Identity=51.3888888888889, Blast_Score=159, Evalue=9e-40,
Organism=Drosophila melanogaster, GI18859983, Length=142, Percent_Identity=51.4084507042254, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24642371, Length=142, Percent_Identity=51.4084507042254, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651058, Length=142, Percent_Identity=51.4084507042254, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_LEPBA (B0SFT5)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962343.1
- ProteinModelPortal:   B0SFT5
- SMR:   B0SFT5
- GeneID:   6388332
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1243
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- ProtClustDB:   PRK14814
- BioCyc:   LBIF355278:LBF_1243-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20600; Mature: 20469

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLTETLSKPGEMFGDMFQVATLDNVVQWGQSFSLWPYPFATACCGIEYMSTACADYDIA
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQLAEPKFVISVGACASSGGMFHTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
GVLQGVDRILPVDVYVPGCPPRPEAILDALVKLQKKVQSQGLEARRQEVMRKIEEINERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVA
CCEEEC
>Mature Secondary Structure 
GLTETLSKPGEMFGDMFQVATLDNVVQWGQSFSLWPYPFATACCGIEYMSTACADYDIA
CCHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQLAEPKFVISVGACASSGGMFHTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHH
GVLQGVDRILPVDVYVPGCPPRPEAILDALVKLQKKVQSQGLEARRQEVMRKIEEINERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVA
CCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA