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The map label for this gene is bioF [C]

Identifier: 183220134

GI number: 183220134

Start: 736095

End: 737264

Strand: Direct

Name: bioF [C]

Synonym: LEPBI_I0722

Alternate gene names: 183220134

Gene position: 736095-737264 (Clockwise)

Preceding gene: 183220131

Following gene: 183220140

Centisome position: 20.45

GC content: 40.85

Gene sequence:

>1170_bases
GTGAACCGTCATTGGCAAGAAATTCAAAAAAAATTGGAATCCATCAAGGAAAAACAATTGTTCCGGGAAACCAAATCTTA
CCAAGGCATTGATTTTTGTTCGAATGATTATATGGGTCTAACATCTAACCCGAATCTGTTAGAATACTTTGAATCCTTGA
AAGATGAGTATCCCTTTGGTTCGACTGCGTCTCGCCTTGTCCGAGGGAATTATGATTCCATGGACCAATTCGAACGTGAA
TTTGCAAACTTTGTTTATGGAGAAGCGGCACTCCTCGTGTCAACTGGGTTTGTGGCCAATTTTGGGCTAATTGATTCAAT
TGCAGCGCCTGATTGTTATGTGTTTTGCGACCGTTTGAACCATGCTTCCATCTTAGATGGAATTCGAATTTCAGGTGCCA
AAAAAAAATACTACAACCATTTAGACTTACAACATTTGAAATCATTATTGGATAAGGCCGACAAAGAAGATCCGCAAAAA
AAACAGAAACGCATCGTCGTGACCGAATCTCTTTTTGGTATGGATGGGGATAGTCCTGATTTTAAAACTCTCCTCAAACT
CAAAGAAGAATACGACTTTGTTCTCGTTGTGGATGAAGCGCATTCATTGGGTGTGTATGGGCCAGAAGGAAAAGGGATTT
TATTTCGTGACTTAACAATGGATGACATCCAATCCATTGACTACCGAGTGTATACCTTGGGAAAATCATTTGGTTTGGAA
GGTGGGATTATTGTTACCAAAAAAATGGGACGTGATCACCTCGTCAATGTGATGCGGCCTTTCATTTTTTCCACAGCACC
ACTTCCGATTGTATCCAAATTAGCAATCTTTGCTTTAGAACTTTTAAGGTCGATGGATACTTTGCGTTCTGAGTTACATA
CTCTGTCCGTTGATTTTAAAAATTCTCTTTTGGCGATTGGGTTTTCGATCACGAGTACCGAAACACATATCGTTCCATTG
TTATTACCATCTGAAAGTGAAGCTTTGTATTATGCCAAACGATTGCAGGAGATGGGTCTTGATGTGCGCGCGATTCGTCC
ACCAACGGTTCCCACACCTAGATTACGGATCAGTTTGAATGCCAAATTGACAAAAAAAGACACAGAGGCCTTGGTTACGG
CTCTCGTCCAAATCCGTAAGGATTGGGATGAGTCTGTTCCTTTGGTGTGA

Upstream 100 bases:

>100_bases
AAAGAATCTTGGGGCAAAAGGTTCATTTCTTTGGTTCATTCTAACAAATTCTTTCAAAATTTAAATCGGAAAAAATTTCA
GTCTAGGAATTCTGGTCGTT

Downstream 100 bases:

>100_bases
AATTTTTAAAGGAACAAAAGTTCGCTCGCGATTGAGATCATTCTCCTCGGCCCGAACCAAAATTGAAACCGAGGGAGGAA
TTTGTAATCTTCTGTTATGA

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS/AKB ligase; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; Alpha-oxoamine synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNRHWQEIQKKLESIKEKQLFRETKSYQGIDFCSNDYMGLTSNPNLLEYFESLKDEYPFGSTASRLVRGNYDSMDQFERE
FANFVYGEAALLVSTGFVANFGLIDSIAAPDCYVFCDRLNHASILDGIRISGAKKKYYNHLDLQHLKSLLDKADKEDPQK
KQKRIVVTESLFGMDGDSPDFKTLLKLKEEYDFVLVVDEAHSLGVYGPEGKGILFRDLTMDDIQSIDYRVYTLGKSFGLE
GGIIVTKKMGRDHLVNVMRPFIFSTAPLPIVSKLAIFALELLRSMDTLRSELHTLSVDFKNSLLAIGFSITSTETHIVPL
LLPSESEALYYAKRLQEMGLDVRAIRPPTVPTPRLRISLNAKLTKKDTEALVTALVQIRKDWDESVPLV

Sequences:

>Translated_389_residues
MNRHWQEIQKKLESIKEKQLFRETKSYQGIDFCSNDYMGLTSNPNLLEYFESLKDEYPFGSTASRLVRGNYDSMDQFERE
FANFVYGEAALLVSTGFVANFGLIDSIAAPDCYVFCDRLNHASILDGIRISGAKKKYYNHLDLQHLKSLLDKADKEDPQK
KQKRIVVTESLFGMDGDSPDFKTLLKLKEEYDFVLVVDEAHSLGVYGPEGKGILFRDLTMDDIQSIDYRVYTLGKSFGLE
GGIIVTKKMGRDHLVNVMRPFIFSTAPLPIVSKLAIFALELLRSMDTLRSELHTLSVDFKNSLLAIGFSITSTETHIVPL
LLPSESEALYYAKRLQEMGLDVRAIRPPTVPTPRLRISLNAKLTKKDTEALVTALVQIRKDWDESVPLV
>Mature_389_residues
MNRHWQEIQKKLESIKEKQLFRETKSYQGIDFCSNDYMGLTSNPNLLEYFESLKDEYPFGSTASRLVRGNYDSMDQFERE
FANFVYGEAALLVSTGFVANFGLIDSIAAPDCYVFCDRLNHASILDGIRISGAKKKYYNHLDLQHLKSLLDKADKEDPQK
KQKRIVVTESLFGMDGDSPDFKTLLKLKEEYDFVLVVDEAHSLGVYGPEGKGILFRDLTMDDIQSIDYRVYTLGKSFGLE
GGIIVTKKMGRDHLVNVMRPFIFSTAPLPIVSKLAIFALELLRSMDTLRSELHTLSVDFKNSLLAIGFSITSTETHIVPL
LLPSESEALYYAKRLQEMGLDVRAIRPPTVPTPRLRISLNAKLTKKDTEALVTALVQIRKDWDESVPLV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A and/or converts 2-amino-3-ketobutyrate to glycine and acetyl-CoA [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=357, Percent_Identity=31.0924369747899, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI284448556, Length=357, Percent_Identity=31.0924369747899, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI83977444, Length=325, Percent_Identity=32, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI83977442, Length=325, Percent_Identity=32, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI83977440, Length=325, Percent_Identity=32, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4502025, Length=326, Percent_Identity=30.9815950920245, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI40316939, Length=326, Percent_Identity=30.9815950920245, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4758668, Length=363, Percent_Identity=28.9256198347107, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI119220554, Length=363, Percent_Identity=28.099173553719, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI5454084, Length=248, Percent_Identity=27.8225806451613, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1790046, Length=393, Percent_Identity=29.5165394402036, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=31.9209039548023, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71994529, Length=398, Percent_Identity=30.1507537688442, Blast_Score=171, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI32566772, Length=374, Percent_Identity=25.4010695187166, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=352, Percent_Identity=25.2840909090909, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560914, Length=352, Percent_Identity=25.2840909090909, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71982617, Length=237, Percent_Identity=27.4261603375527, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982625, Length=237, Percent_Identity=27.4261603375527, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320438, Length=368, Percent_Identity=28.804347826087, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320267, Length=369, Percent_Identity=27.6422764227642, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=269, Percent_Identity=24.907063197026, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24662918, Length=357, Percent_Identity=29.1316526610644, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI17137420, Length=371, Percent_Identity=32.0754716981132, Blast_Score=159, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17136286, Length=334, Percent_Identity=25.4491017964072, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653280, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653276, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24653278, Length=263, Percent_Identity=25.0950570342205, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.29; =2.3.1.47 [H]

Molecular weight: Translated: 44130; Mature: 44130

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRHWQEIQKKLESIKEKQLFRETKSYQGIDFCSNDYMGLTSNPNLLEYFESLKDEYPFG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
STASRLVRGNYDSMDQFEREFANFVYGEAALLVSTGFVANFGLIDSIAAPDCYVFCDRLN
CHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEEECCHHHCCCHHHHCCCCCHHHHHHHCC
HASILDGIRISGAKKKYYNHLDLQHLKSLLDKADKEDPQKKQKRIVVTESLFGMDGDSPD
CHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCEEEEEHHHHCCCCCCHH
FKTLLKLKEEYDFVLVVDEAHSLGVYGPEGKGILFRDLTMDDIQSIDYRVYTLGKSFGLE
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCEEEEEECHHCCCC
GGIIVTKKMGRDHLVNVMRPFIFSTAPLPIVSKLAIFALELLRSMDTLRSELHTLSVDFK
CCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NSLLAIGFSITSTETHIVPLLLPSESEALYYAKRLQEMGLDVRAIRPPTVPTPRLRISLN
CCEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEC
AKLTKKDTEALVTALVQIRKDWDESVPLV
CEECHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNRHWQEIQKKLESIKEKQLFRETKSYQGIDFCSNDYMGLTSNPNLLEYFESLKDEYPFG
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
STASRLVRGNYDSMDQFEREFANFVYGEAALLVSTGFVANFGLIDSIAAPDCYVFCDRLN
CHHHHHHCCCCCCHHHHHHHHHHHHHCCHHEEEECCHHHCCCHHHHCCCCCHHHHHHHCC
HASILDGIRISGAKKKYYNHLDLQHLKSLLDKADKEDPQKKQKRIVVTESLFGMDGDSPD
CHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHCEEEEEHHHHCCCCCCHH
FKTLLKLKEEYDFVLVVDEAHSLGVYGPEGKGILFRDLTMDDIQSIDYRVYTLGKSFGLE
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHCCEEEEEECHHCCCC
GGIIVTKKMGRDHLVNVMRPFIFSTAPLPIVSKLAIFALELLRSMDTLRSELHTLSVDFK
CCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
NSLLAIGFSITSTETHIVPLLLPSESEALYYAKRLQEMGLDVRAIRPPTVPTPRLRISLN
CCEEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEC
AKLTKKDTEALVTALVQIRKDWDESVPLV
CEECHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA