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The map label for this gene is dfpB [H]

Identifier: 183220131

GI number: 183220131

Start: 734090

End: 734767

Strand: Direct

Name: dfpB [H]

Synonym: LEPBI_I0719

Alternate gene names: 183220131

Gene position: 734090-734767 (Clockwise)

Preceding gene: 183220130

Following gene: 183220134

Centisome position: 20.39

GC content: 40.56

Gene sequence:

>678_bases
ATGAATTTAAAATTCAAACGAGTCATTGTCACCTCAGGACCCACGAGAGAATGGATTGACCCAGTTCGGTACATCTCGAA
TGCCTCATCTGGCAAAATGGGATATGAAATTGCAAAATCATTTTTACAATATCCAGTGGATGTGATCTACATCCATGGAA
ATACCTTAGAGCGATATGCCAATCTTCCTGGCGCCAAACAAAACGTAGAGGTTGAAACCACAATGCAACTTCGAGATGCG
GTACTCGCACAAATGGAAAATGATACGCTACTTGTGATGGCCGCAGCTCCCGCTGACTTTCGACCCATCATGACCGCAGA
ACATAAAATCAAAAAAGAAAGATCCTCGGAAGGGAGTAAGGGACTTTTACTCGAGTTAGAAGAAAATCCCGATGTTTTGA
AACAAGTGTTTGAATATGTATCTGAAAACCAAATTCAAAACTCCATCCGTGTGGGTTTTGCCGCTGAAACAAACGATTTA
GAAAAACATGCCAAGGACAAACTTGTTCGCAAAGGATTACAATTCATCGTTGGAAATTATGTGGGTCATGGCAAAGGATT
TGGAGAAGTGGATTCTACTTTGCGTATTTACAGTCAAGTAGGTCTCGTCAAAGAAATTGGTCCCATGCCAAAAGAAAACT
TAGCTGAATCACTTGTTCAATTTTTAGTGACTGTTTGA

Upstream 100 bases:

>100_bases
CAGAAAAGGGCATAGTGGTTTGTGGTGATGAAGGGTATGGCAAACTGGCGACAGTCGAAACCATTATGGAACAAATCATC
GAACTCCACAAAAAGAATTC

Downstream 100 bases:

>100_bases
ATGATTGCCAAATCCAAAGGCAAAATATCCAGCAAGTAAAATCAAACCAAAACTCACCACATAGTTCCATTTGATTCTTT
CACCAAGAAATAAGGTGGCA

Product: phosphopantothenate--cysteine ligase

Products: NA

Alternate protein names: DNA/pantothenate metabolism flavoprotein; Phosphopantothenoylcysteine decarboxylase; PPCDC; CoaC; Phosphopantothenate--cysteine ligase; CoaB; PPC synthetase; PPCS; Phosphopantothenoylcysteine synthase [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNLKFKRVIVTSGPTREWIDPVRYISNASSGKMGYEIAKSFLQYPVDVIYIHGNTLERYANLPGAKQNVEVETTMQLRDA
VLAQMENDTLLVMAAAPADFRPIMTAEHKIKKERSSEGSKGLLLELEENPDVLKQVFEYVSENQIQNSIRVGFAAETNDL
EKHAKDKLVRKGLQFIVGNYVGHGKGFGEVDSTLRIYSQVGLVKEIGPMPKENLAESLVQFLVTV

Sequences:

>Translated_225_residues
MNLKFKRVIVTSGPTREWIDPVRYISNASSGKMGYEIAKSFLQYPVDVIYIHGNTLERYANLPGAKQNVEVETTMQLRDA
VLAQMENDTLLVMAAAPADFRPIMTAEHKIKKERSSEGSKGLLLELEENPDVLKQVFEYVSENQIQNSIRVGFAAETNDL
EKHAKDKLVRKGLQFIVGNYVGHGKGFGEVDSTLRIYSQVGLVKEIGPMPKENLAESLVQFLVTV
>Mature_225_residues
MNLKFKRVIVTSGPTREWIDPVRYISNASSGKMGYEIAKSFLQYPVDVIYIHGNTLERYANLPGAKQNVEVETTMQLRDA
VLAQMENDTLLVMAAAPADFRPIMTAEHKIKKERSSEGSKGLLLELEENPDVLKQVFEYVSENQIQNSIRVGFAAETNDL
EKHAKDKLVRKGLQFIVGNYVGHGKGFGEVDSTLRIYSQVGLVKEIGPMPKENLAESLVQFLVTV

Specific function: Catalyzes two steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine, in the latter compound is decarboxylated to form 4'-phosphopantotheine [H]

COG id: COG0452

COG function: function code H; Phosphopantothenoylcysteine synthetase/decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PPC synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87082301, Length=227, Percent_Identity=34.8017621145374, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005252
- InterPro:   IPR007085
- InterPro:   IPR003382 [H]

Pfam domain/function: PF04127 DFP; PF02441 Flavoprotein [H]

EC number: =4.1.1.36; =6.3.2.5 [H]

Molecular weight: Translated: 25188; Mature: 25188

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKFKRVIVTSGPTREWIDPVRYISNASSGKMGYEIAKSFLQYPVDVIYIHGNTLERYA
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHH
NLPGAKQNVEVETTMQLRDAVLAQMENDTLLVMAAAPADFRPIMTAEHKIKKERSSEGSK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
GLLLELEENPDVLKQVFEYVSENQIQNSIRVGFAAETNDLEKHAKDKLVRKGLQFIVGNY
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VGHGKGFGEVDSTLRIYSQVGLVKEIGPMPKENLAESLVQFLVTV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLKFKRVIVTSGPTREWIDPVRYISNASSGKMGYEIAKSFLQYPVDVIYIHGNTLERYA
CCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHH
NLPGAKQNVEVETTMQLRDAVLAQMENDTLLVMAAAPADFRPIMTAEHKIKKERSSEGSK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCC
GLLLELEENPDVLKQVFEYVSENQIQNSIRVGFAAETNDLEKHAKDKLVRKGLQFIVGNY
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VGHGKGFGEVDSTLRIYSQVGLVKEIGPMPKENLAESLVQFLVTV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]