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The map label for this gene is ispE

Identifier: 183219663

GI number: 183219663

Start: 228923

End: 229843

Strand: Reverse

Name: ispE

Synonym: LEPBI_I0238

Alternate gene names: 183219663

Gene position: 229843-228923 (Counterclockwise)

Preceding gene: 183219664

Following gene: 183219662

Centisome position: 6.39

GC content: 43.21

Gene sequence:

>921_bases
TTGGTTCTTTTGCAAACGATTGCACATGCAAAGATTAACATTGGTTTGATGATTCCTTATAAAAGGGAAGATGGACTCCA
TGAAATCCGAAGTGTCTTTGTTCCCATTGACTTTGGTGATCCCATGGAAATCCAAATCAAGTCCTTACCAAAAGGAATTG
TTTCCAGATTTGTATTCCACTCCACAAACCATTTACAAGGGTATCGCCATTCCCTTTTTGAAGCTGTCTCGGAACGTGGG
GACCTTTCCCAAAATATCCTCACCAAAACCTTTCATAAACTGAAACCCTACTTCCAAGAAGCTTTGGAAATCAAGGTCCA
AATACAAAAACACCTGCCTCCCGAAGGGGGAATTGGCGGTGGGAGTAGCAATGCGGGTGTTTTATTACAACACCTGTTTC
CCTATACGAATCTTTCGCGGGGAGAACAAATTCAATTTGCCAAATCCATCGGCGCGGATGTTCCTTTTTTCCTCCAATCG
AGTGCCTGTTTTGTGACTGGGATTGGGGAAGTGATGGAACCCATCCGTTTGGCCAAGGGTTTTGGGATTTTGGCCATCCC
TCCCTTTGGATTATCTACCGGTTCTATGTACGCAAGTCTCCAAAAAAGTTTACAAAAACCCTATGGTTCCGAAGTATGGA
AATCTCTGACAGAGGATTTAATTCGAAGTCTTCACGTCGGGGATTGGGTTTACCTGCAAAACAGGCTAGAGAACGAGTTT
GAAAAAATCGCTTTCCAGACCCAACCCTTACTAAAGGAATTGAAATTAGGGTTCTTTGAGTCGGGAGCAATTTTTGCGTC
TCTTTCTGGTTCTGGATCATGTCTTTATGGCATTTATCCGACCGAAGAGAAACGAAACGAAGCCCTTCCCAATGTCTCTC
TTAGGTTCCCGAAAATGGAATTTCGAACGTTCTCTTTTTAG

Upstream 100 bases:

>100_bases
TCCATTCTTGTGTCCGAACCAAATGAAATCAAAGAATCCATCAGACTGCATTTACAATCCATTTCCCTTTCTAAAGAAAA
CAAGTCGATAGAAACGTAAG

Downstream 100 bases:

>100_bases
AAACTGGCCTGTAGCCAAGCGGTAAGGCAGCGGTTTTTGGTATCGCCATTCCCTAGGTTCGAATCCTAGCAGGCCAGCCA
GTTTTAAAAGATCCGATAGA

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MVLLQTIAHAKINIGLMIPYKREDGLHEIRSVFVPIDFGDPMEIQIKSLPKGIVSRFVFHSTNHLQGYRHSLFEAVSERG
DLSQNILTKTFHKLKPYFQEALEIKVQIQKHLPPEGGIGGGSSNAGVLLQHLFPYTNLSRGEQIQFAKSIGADVPFFLQS
SACFVTGIGEVMEPIRLAKGFGILAIPPFGLSTGSMYASLQKSLQKPYGSEVWKSLTEDLIRSLHVGDWVYLQNRLENEF
EKIAFQTQPLLKELKLGFFESGAIFASLSGSGSCLYGIYPTEEKRNEALPNVSLRFPKMEFRTFSF

Sequences:

>Translated_306_residues
MVLLQTIAHAKINIGLMIPYKREDGLHEIRSVFVPIDFGDPMEIQIKSLPKGIVSRFVFHSTNHLQGYRHSLFEAVSERG
DLSQNILTKTFHKLKPYFQEALEIKVQIQKHLPPEGGIGGGSSNAGVLLQHLFPYTNLSRGEQIQFAKSIGADVPFFLQS
SACFVTGIGEVMEPIRLAKGFGILAIPPFGLSTGSMYASLQKSLQKPYGSEVWKSLTEDLIRSLHVGDWVYLQNRLENEF
EKIAFQTQPLLKELKLGFFESGAIFASLSGSGSCLYGIYPTEEKRNEALPNVSLRFPKMEFRTFSF
>Mature_306_residues
MVLLQTIAHAKINIGLMIPYKREDGLHEIRSVFVPIDFGDPMEIQIKSLPKGIVSRFVFHSTNHLQGYRHSLFEAVSERG
DLSQNILTKTFHKLKPYFQEALEIKVQIQKHLPPEGGIGGGSSNAGVLLQHLFPYTNLSRGEQIQFAKSIGADVPFFLQS
SACFVTGIGEVMEPIRLAKGFGILAIPPFGLSTGSMYASLQKSLQKPYGSEVWKSLTEDLIRSLHVGDWVYLQNRLENEF
EKIAFQTQPLLKELKLGFFESGAIFASLSGSGSCLYGIYPTEEKRNEALPNVSLRFPKMEFRTFSF

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=288, Percent_Identity=26.0416666666667, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_LEPBA (B0SA95)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001961355.1
- ProteinModelPortal:   B0SA95
- SMR:   B0SA95
- GeneID:   6388987
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_0232
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK14613
- BioCyc:   LBIF355278:LBF_0232-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF08544 GHMP_kinases_C; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 34276; Mature: 34276

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 153-153

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLQTIAHAKINIGLMIPYKREDGLHEIRSVFVPIDFGDPMEIQIKSLPKGIVSRFVFH
CHHHHHHHHCEEEEEEEEEECCCCCHHHHHHEEEECCCCCCCEEEHHHCCHHHHHHHHHH
STNHLQGYRHSLFEAVSERGDLSQNILTKTFHKLKPYFQEALEIKVQIQKHLPPEGGIGG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSSNAGVLLQHLFPYTNLSRGEQIQFAKSIGADVPFFLQSSACFVTGIGEVMEPIRLAKG
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCCCEEECHHHHHHHHHHHCC
FGILAIPPFGLSTGSMYASLQKSLQKPYGSEVWKSLTEDLIRSLHVGDWVYLQNRLENEF
CCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EKIAFQTQPLLKELKLGFFESGAIFASLSGSGSCLYGIYPTEEKRNEALPNVSLRFPKME
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCCEEECCCCC
FRTFSF
CEECCC
>Mature Secondary Structure
MVLLQTIAHAKINIGLMIPYKREDGLHEIRSVFVPIDFGDPMEIQIKSLPKGIVSRFVFH
CHHHHHHHHCEEEEEEEEEECCCCCHHHHHHEEEECCCCCCCEEEHHHCCHHHHHHHHHH
STNHLQGYRHSLFEAVSERGDLSQNILTKTFHKLKPYFQEALEIKVQIQKHLPPEGGIGG
CCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GSSNAGVLLQHLFPYTNLSRGEQIQFAKSIGADVPFFLQSSACFVTGIGEVMEPIRLAKG
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCHHHCCCCCEEECHHHHHHHHHHHCC
FGILAIPPFGLSTGSMYASLQKSLQKPYGSEVWKSLTEDLIRSLHVGDWVYLQNRLENEF
CCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EKIAFQTQPLLKELKLGFFESGAIFASLSGSGSCLYGIYPTEEKRNEALPNVSLRFPKME
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCCCEEECCCCC
FRTFSF
CEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA