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The map label for this gene is ycjG [H]

Identifier: 182680315

GI number: 182680315

Start: 3874269

End: 3875249

Strand: Reverse

Name: ycjG [H]

Synonym: Bind_3415

Alternate gene names: 182680315

Gene position: 3875249-3874269 (Counterclockwise)

Preceding gene: 182680316

Following gene: 182680314

Centisome position: 92.93

GC content: 62.59

Gene sequence:

>981_bases
ATGCCGCGCCTAACCCTGGCGATCGAATCTTTCCCCCTGGCCAGTCGTTTCGTCATCTCGCGTGGGGCGAAAAGCGTGGC
GGAGGTCGTTGTCGCCACGCTTGACTCTGGACATGCCAGGGGACGGGGAGAATGTGTGCCCTATGCGCGCTACGGCGAAA
GCCCAGCGAGCGTCCTCGCTCAAATCGAGATGATCCGCCCCGCGATTGAAGCCGGCGCTGGGCGTGAGGTTTTGCAGGAG
CTTCTGCCGCCAGGGGCCGCCCGTAATGCGCTCGATTGCGCTTTGTGGGATCTGGAAGCCAAGGAAACCGGTGTGCCCGT
CTATCGTCGCGCGGGCCTTTCGGAGCCCGTGCCCGCCGTCACGGCTTATACGATCTCGGTCGGCACGCCTGAAACCATGG
CGGAAGCCGCGGCCAAGGCCAGCCATCGACCCTTGCTGAAACTCAAGCTCGCAGGGGAAGGGGATCTTGCCCGCATTGCC
GCCGTGCGCGCGGCGGCGCCCGAGGCGACCCTGATCGTCGATGCCAACGAGGCTTGGACCGAGACAATGCTTGGCCCCTA
TTTCGAGGCTTGCGCGGCGGCTGGTGTCAAACTGATCGAACAGCCCTTGCCAGCCGGCCAGGATGCGATGCTTGCAAAAA
TTTCAAGGCCCGTGTCCATTTGTGCGGATGAAAGCGCTCATGATCGCGATGGACTCGAGGACCTTGCGGCCCGCTATGAT
ATGATCAATGTCAAACTGGATAAAACCGGTGGTCTGACGGAGGCCCTGTGTCTCGTCGAGGCGGCGCGCGGGTTGAAACT
CGATATCATGCTTGGATGCATGGTGGCGAGTTCGCTGGCCATGGCCCCCGCCATGCTGCTTGCGTCACAAGCCCGGTTCG
TCGATCTCGATGGCCCCCTGCTGCTTGCGAAGGATCGTCCCCATGGGCTCACCTATGAGGGCTCCCTTGCTTATCCGCCC
GCGCCCGATCTTTGGGGATGA

Upstream 100 bases:

>100_bases
GCGGAAGAGGCTTTAGCCAAGAAAAACATCAAACGCAGCCGCCGGCGCTGACCGTTTTTTCTCCTTCCTTTCCCACGAGC
CTTTCAAGAGACGTCCCACC

Downstream 100 bases:

>100_bases
GTGCGATGGGAAAAAGCCATTTGATAGATTATCGATCGACGCGGAGCCATTATGCAGCCTCAAGGTGAAGTCGTCGTCAC
ATCGGGCGATATTATCGTCT

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPRLTLAIESFPLASRFVISRGAKSVAEVVVATLDSGHARGRGECVPYARYGESPASVLAQIEMIRPAIEAGAGREVLQE
LLPPGAARNALDCALWDLEAKETGVPVYRRAGLSEPVPAVTAYTISVGTPETMAEAAAKASHRPLLKLKLAGEGDLARIA
AVRAAAPEATLIVDANEAWTETMLGPYFEACAAAGVKLIEQPLPAGQDAMLAKISRPVSICADESAHDRDGLEDLAARYD
MINVKLDKTGGLTEALCLVEAARGLKLDIMLGCMVASSLAMAPAMLLASQARFVDLDGPLLLAKDRPHGLTYEGSLAYPP
APDLWG

Sequences:

>Translated_326_residues
MPRLTLAIESFPLASRFVISRGAKSVAEVVVATLDSGHARGRGECVPYARYGESPASVLAQIEMIRPAIEAGAGREVLQE
LLPPGAARNALDCALWDLEAKETGVPVYRRAGLSEPVPAVTAYTISVGTPETMAEAAAKASHRPLLKLKLAGEGDLARIA
AVRAAAPEATLIVDANEAWTETMLGPYFEACAAAGVKLIEQPLPAGQDAMLAKISRPVSICADESAHDRDGLEDLAARYD
MINVKLDKTGGLTEALCLVEAARGLKLDIMLGCMVASSLAMAPAMLLASQARFVDLDGPLLLAKDRPHGLTYEGSLAYPP
APDLWG
>Mature_325_residues
PRLTLAIESFPLASRFVISRGAKSVAEVVVATLDSGHARGRGECVPYARYGESPASVLAQIEMIRPAIEAGAGREVLQEL
LPPGAARNALDCALWDLEAKETGVPVYRRAGLSEPVPAVTAYTISVGTPETMAEAAAKASHRPLLKLKLAGEGDLARIAA
VRAAAPEATLIVDANEAWTETMLGPYFEACAAAGVKLIEQPLPAGQDAMLAKISRPVSICADESAHDRDGLEDLAARYDM
INVKLDKTGGLTEALCLVEAARGLKLDIMLGCMVASSLAMAPAMLLASQARFVDLDGPLLLAKDRPHGLTYEGSLAYPPA
PDLWG

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=306, Percent_Identity=48.6928104575163, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34292; Mature: 34161

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLTLAIESFPLASRFVISRGAKSVAEVVVATLDSGHARGRGECVPYARYGESPASVLA
CCCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHH
QIEMIRPAIEAGAGREVLQELLPPGAARNALDCALWDLEAKETGVPVYRRAGLSEPVPAV
HHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCCHHHHCCCCCCCCCE
TAYTISVGTPETMAEAAAKASHRPLLKLKLAGEGDLARIAAVRAAAPEATLIVDANEAWT
EEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHHH
ETMLGPYFEACAAAGVKLIEQPLPAGQDAMLAKISRPVSICADESAHDRDGLEDLAARYD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHE
MINVKLDKTGGLTEALCLVEAARGLKLDIMLGCMVASSLAMAPAMLLASQARFVDLDGPL
EEEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCE
LLAKDRPHGLTYEGSLAYPPAPDLWG
EEECCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure 
PRLTLAIESFPLASRFVISRGAKSVAEVVVATLDSGHARGRGECVPYARYGESPASVLA
CCEEEEEECCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCHHHHHH
QIEMIRPAIEAGAGREVLQELLPPGAARNALDCALWDLEAKETGVPVYRRAGLSEPVPAV
HHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHEEECCCCCCCCCHHHHCCCCCCCCCE
TAYTISVGTPETMAEAAAKASHRPLLKLKLAGEGDLARIAAVRAAAPEATLIVDANEAWT
EEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCHHHH
ETMLGPYFEACAAAGVKLIEQPLPAGQDAMLAKISRPVSICADESAHDRDGLEDLAARYD
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHE
MINVKLDKTGGLTEALCLVEAARGLKLDIMLGCMVASSLAMAPAMLLASQARFVDLDGPL
EEEEEECCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCE
LLAKDRPHGLTYEGSLAYPPAPDLWG
EEECCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]