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The map label for this gene is rbsB [H]

Identifier: 182679773

GI number: 182679773

Start: 3264953

End: 3265882

Strand: Reverse

Name: rbsB [H]

Synonym: Bind_2861

Alternate gene names: 182679773

Gene position: 3265882-3264953 (Counterclockwise)

Preceding gene: 182679774

Following gene: 182679772

Centisome position: 78.32

GC content: 51.61

Gene sequence:

>930_bases
ATGAAAAAGGTGTTAATGAGTGCCGTGGCGATTGTTGCTCTCATGACGGCTCAAGCGTCTGCTAAAGAGTTGAAATCTAT
TGGGCTTTCGCTCGGCTCCATGGGCAATCCCTTTTTTGTTGCAATGGCCAAGGGTGCGGCAGCGAGTGCTCAGAAAATCA
ATCCGAATGCCAAGGTGACAGCGCTAGGTTTCGATTATGACCTGAATAAGCAATTCACGCAGATCGATAATTTCATCGCC
TCCGGCGTTGATCTGATTCTGCTCAACCCCGGTGACCCTGTGGCTCTGGAGCCAGCGATCAAACGCGCCCAAGCGGCGGG
TATCCCTGTCATCGCGGTCGATACAGCTGCCAAGGGCGCCGATATCGTGATCACCACGGACAATACGCAGGCTGGTGAAA
TTTCCTGCCAATATATTGTGGATAAGCTCGGCGGCAAAGGTGATGTCATCATCGAGAACGGTCCGCAGGTGTCTTCCGTG
CGCGAGCGTGTGGCCGGTTGCAAGAAGGCGCTGAGTAATGCGCCAGGCATCAAAATCCTTTCAAGTGATCAAGATGCTAA
AGGATCGCGTGATCAAGGCTTGAACGTGATGCAGGGCTATCTCACCCGCTTCCCCAAGATCGACGCGGTCTTCACGATCA
ATGATCCGCAAGCGATCGGCTCCGATCTCGCTGTTAAACAGCTTCATCGTGACCAGATTATGATTACGTCGGTTGATGGT
GCACCCGATATCGAAGTCGCTCTCAAAGCAAATACACATATTCATGCTTCTGCAAGCCAGGACCCTTATGCAATCGCACA
ACTTGCGGTCAAGCTTGGGAGTGAGTTGCTGAATGGTAACAAGCCCGAACAGAATGTGATTCTGATTCCCTCCAAACTTG
TGACACGTGATAATGTCGCCGAATATAAAGGCTGGCAGGCTGAGCGTTGA

Upstream 100 bases:

>100_bases
CGCTTGAAGGGTTCTGCCCGTACCTGACAGGCTCATGGTTCGTCACGAAAATTGGCGGTTCGGGCGATAAAAAAGACCAA
TAATGGTGGAGGGAATGAAT

Downstream 100 bases:

>100_bases
TCAATTGAGTCTCAGGGGAAGATAAAAAGCTTCATGCCGGTGAAAAGCCAGAAGCAAGAAGAAATGGCATTGGCATTGGC
ATGAAGCTGAGGGAGGGGAA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKVLMSAVAIVALMTAQASAKELKSIGLSLGSMGNPFFVAMAKGAAASAQKINPNAKVTALGFDYDLNKQFTQIDNFIA
SGVDLILLNPGDPVALEPAIKRAQAAGIPVIAVDTAAKGADIVITTDNTQAGEISCQYIVDKLGGKGDVIIENGPQVSSV
RERVAGCKKALSNAPGIKILSSDQDAKGSRDQGLNVMQGYLTRFPKIDAVFTINDPQAIGSDLAVKQLHRDQIMITSVDG
APDIEVALKANTHIHASASQDPYAIAQLAVKLGSELLNGNKPEQNVILIPSKLVTRDNVAEYKGWQAER

Sequences:

>Translated_309_residues
MKKVLMSAVAIVALMTAQASAKELKSIGLSLGSMGNPFFVAMAKGAAASAQKINPNAKVTALGFDYDLNKQFTQIDNFIA
SGVDLILLNPGDPVALEPAIKRAQAAGIPVIAVDTAAKGADIVITTDNTQAGEISCQYIVDKLGGKGDVIIENGPQVSSV
RERVAGCKKALSNAPGIKILSSDQDAKGSRDQGLNVMQGYLTRFPKIDAVFTINDPQAIGSDLAVKQLHRDQIMITSVDG
APDIEVALKANTHIHASASQDPYAIAQLAVKLGSELLNGNKPEQNVILIPSKLVTRDNVAEYKGWQAER
>Mature_309_residues
MKKVLMSAVAIVALMTAQASAKELKSIGLSLGSMGNPFFVAMAKGAAASAQKINPNAKVTALGFDYDLNKQFTQIDNFIA
SGVDLILLNPGDPVALEPAIKRAQAAGIPVIAVDTAAKGADIVITTDNTQAGEISCQYIVDKLGGKGDVIIENGPQVSSV
RERVAGCKKALSNAPGIKILSSDQDAKGSRDQGLNVMQGYLTRFPKIDAVFTINDPQAIGSDLAVKQLHRDQIMITSVDG
APDIEVALKANTHIHASASQDPYAIAQLAVKLGSELLNGNKPEQNVILIPSKLVTRDNVAEYKGWQAER

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=286, Percent_Identity=34.965034965035, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1790526, Length=274, Percent_Identity=27.3722627737226, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790674, Length=191, Percent_Identity=31.413612565445, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32557; Mature: 32557

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLMSAVAIVALMTAQASAKELKSIGLSLGSMGNPFFVAMAKGAAASAQKINPNAKVT
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCEEE
ALGFDYDLNKQFTQIDNFIASGVDLILLNPGDPVALEPAIKRAQAAGIPVIAVDTAAKGA
EEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHCCCCEEEEECCCCCC
DIVITTDNTQAGEISCQYIVDKLGGKGDVIIENGPQVSSVRERVAGCKKALSNAPGIKIL
EEEEECCCCCCCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEE
SSDQDAKGSRDQGLNVMQGYLTRFPKIDAVFTINDPQAIGSDLAVKQLHRDQIMITSVDG
ECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHCCCEEEEEECCC
APDIEVALKANTHIHASASQDPYAIAQLAVKLGSELLNGNKPEQNVILIPSKLVTRDNVA
CCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCHH
EYKGWQAER
HHCCCCCCC
>Mature Secondary Structure
MKKVLMSAVAIVALMTAQASAKELKSIGLSLGSMGNPFFVAMAKGAAASAQKINPNAKVT
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCEEE
ALGFDYDLNKQFTQIDNFIASGVDLILLNPGDPVALEPAIKRAQAAGIPVIAVDTAAKGA
EEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECHHHHHHHHCCCCEEEEECCCCCC
DIVITTDNTQAGEISCQYIVDKLGGKGDVIIENGPQVSSVRERVAGCKKALSNAPGIKIL
EEEEECCCCCCCCEEHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEE
SSDQDAKGSRDQGLNVMQGYLTRFPKIDAVFTINDPQAIGSDLAVKQLHRDQIMITSVDG
ECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHCCHHHHHHHCCCEEEEEECCC
APDIEVALKANTHIHASASQDPYAIAQLAVKLGSELLNGNKPEQNVILIPSKLVTRDNVA
CCCEEEEEEECCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCHH
EYKGWQAER
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]