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The map label for this gene is shiA [H]

Identifier: 182679480

GI number: 182679480

Start: 2890731

End: 2892050

Strand: Reverse

Name: shiA [H]

Synonym: Bind_2535

Alternate gene names: 182679480

Gene position: 2892050-2890731 (Counterclockwise)

Preceding gene: 182679481

Following gene: 182679479

Centisome position: 69.35

GC content: 59.32

Gene sequence:

>1320_bases
ATGGTTACCGATGTGAGTGAAACAGCCAGCAAGCGTATCGAACGTCGTGCTGCGATCGCAAGCGTGATCGGCACGACGAT
CGAGTGGTATGACTTCTTCATCTACGGCACAGCGGCTGCACTCATTTTTCCGAAGCTCTATTTCGGCGCTGGTGCATCGG
AAGGCCTGATGGAGTCCTTCAGCACGATTTTCGTCGGGTTCCTGTCTCGACCGATCGGGGCCGCTCTCTTCGGTCACATC
GGAGATCGCGTCGGCCGTAAAGCGACGCTGGTGGCAACTTTGACGCTTATGGGTGTCGCGACCGTCCTCGTAGGGATCTT
ACCAGATCGCACATCGATCGGTGCCTGGGCGGGCTGGGGTCTCGTGGTCCTGCGCTTCTTTCAAGGCATTGGTGTTGGTG
GCGAATGGGGCGGCGCGGTGCTTCTCTCGATGGAGTCCCATCAGGGCGATCGCCGTGGATTTATGGCAAGCCTGCCTCAC
ATCGGTGTCCCTCTCGGCCTCGTTGTCTCTGTGCTCGCCTGGAGTGCCTGCAGCTACTGGTCAGGAGGCAATCTTGAAGA
CGGTGCTTGGCGTATCCCATTCCTCGCAAGCGGTGCTCTGCTGGTGTTTGGACTGGTTGTCCGCCTCGGAGTCCCTGAGA
CACCAGATTTTGTGAAGGCGCAGCGCGAGGTGCGTGCTTCGATGCCACCATTGATCGAAGCAATCAAAACGCAATGGCGT
TCGATCTTGCTTGCCGCCCTCATTCGACCGGGCGAGCAGGCGAGTTTCTACATGCTGTCGACCTTTGTCGTTCTATACGG
CTCCGCGCAGCTCGGCCTTGGCCGGGAGTTCATGCTCAATGCTGTGCTCCTCGGCGCCGTGGCGTCCTGTTTCATGGTGC
CTTACTTTGGCCATCTCGGGGATCGGATCGGTCGTCGTCGGATCTATCTCACCGGCGCGATTGTAATGACGCTGTTCGCG
TTACCGTACTTCTGGCTTCTGGACACGCGCCAGCCGATCCTTGTGATCGTCGCCATCCTTGGGCTGATGGCCGCACATGA
CATGATGTACGGCCCACAGGCCGCATACATCACAGAAAGCTTCCCCGCTCATATCCGGTACACCGGTGCGTCGCTCGGCT
ATCAGAGTGCGTCCATTATTGCGGGAGGACCGGCTCCCCTCGTTTCGCTTTGGCTCTTCAACACTTTTGGGACAGGCTAC
GCCGTGGGCGCCTTTCTCGCTCTGCTTGGGCTGGTCAGTGCGTGTGGCGCTTTCTTCCTCGGACGAGAGCCGCTTCCGGA
GCCGGCGCGAGACCAGCAGCTGCAGCCTCAGGAAGTTTGA

Upstream 100 bases:

>100_bases
TGGAAGAGCTCGGCCTAAGCGCATCCGGCAAAACACGGCTCTACGCCTGATCAGATTCCCGGCCGCAAAACAGAACAAGC
CAGACATGGAGGAATTGCCT

Downstream 100 bases:

>100_bases
GTGTGGTATGTTGGTCATTCATCCCGGAAGAGATGTCGTGACGCTGCACGCTGATGCCACCTTACCAATCGATGAGTCAA
AAGGGCGCTCTCACAAAGTA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MVTDVSETASKRIERRAAIASVIGTTIEWYDFFIYGTAAALIFPKLYFGAGASEGLMESFSTIFVGFLSRPIGAALFGHI
GDRVGRKATLVATLTLMGVATVLVGILPDRTSIGAWAGWGLVVLRFFQGIGVGGEWGGAVLLSMESHQGDRRGFMASLPH
IGVPLGLVVSVLAWSACSYWSGGNLEDGAWRIPFLASGALLVFGLVVRLGVPETPDFVKAQREVRASMPPLIEAIKTQWR
SILLAALIRPGEQASFYMLSTFVVLYGSAQLGLGREFMLNAVLLGAVASCFMVPYFGHLGDRIGRRRIYLTGAIVMTLFA
LPYFWLLDTRQPILVIVAILGLMAAHDMMYGPQAAYITESFPAHIRYTGASLGYQSASIIAGGPAPLVSLWLFNTFGTGY
AVGAFLALLGLVSACGAFFLGREPLPEPARDQQLQPQEV

Sequences:

>Translated_439_residues
MVTDVSETASKRIERRAAIASVIGTTIEWYDFFIYGTAAALIFPKLYFGAGASEGLMESFSTIFVGFLSRPIGAALFGHI
GDRVGRKATLVATLTLMGVATVLVGILPDRTSIGAWAGWGLVVLRFFQGIGVGGEWGGAVLLSMESHQGDRRGFMASLPH
IGVPLGLVVSVLAWSACSYWSGGNLEDGAWRIPFLASGALLVFGLVVRLGVPETPDFVKAQREVRASMPPLIEAIKTQWR
SILLAALIRPGEQASFYMLSTFVVLYGSAQLGLGREFMLNAVLLGAVASCFMVPYFGHLGDRIGRRRIYLTGAIVMTLFA
LPYFWLLDTRQPILVIVAILGLMAAHDMMYGPQAAYITESFPAHIRYTGASLGYQSASIIAGGPAPLVSLWLFNTFGTGY
AVGAFLALLGLVSACGAFFLGREPLPEPARDQQLQPQEV
>Mature_439_residues
MVTDVSETASKRIERRAAIASVIGTTIEWYDFFIYGTAAALIFPKLYFGAGASEGLMESFSTIFVGFLSRPIGAALFGHI
GDRVGRKATLVATLTLMGVATVLVGILPDRTSIGAWAGWGLVVLRFFQGIGVGGEWGGAVLLSMESHQGDRRGFMASLPH
IGVPLGLVVSVLAWSACSYWSGGNLEDGAWRIPFLASGALLVFGLVVRLGVPETPDFVKAQREVRASMPPLIEAIKTQWR
SILLAALIRPGEQASFYMLSTFVVLYGSAQLGLGREFMLNAVLLGAVASCFMVPYFGHLGDRIGRRRIYLTGAIVMTLFA
LPYFWLLDTRQPILVIVAILGLMAAHDMMYGPQAAYITESFPAHIRYTGASLGYQSASIIAGGPAPLVSLWLFNTFGTGY
AVGAFLALLGLVSACGAFFLGREPLPEPARDQQLQPQEV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=419, Percent_Identity=39.618138424821, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1789941, Length=405, Percent_Identity=37.2839506172839, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI1790550, Length=440, Percent_Identity=29.0909090909091, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788942, Length=446, Percent_Identity=29.8206278026906, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082404, Length=400, Percent_Identity=21.5, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47143; Mature: 47143

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTDVSETASKRIERRAAIASVIGTTIEWYDFFIYGTAAALIFPKLYFGAGASEGLMESF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
STIFVGFLSRPIGAALFGHIGDRVGRKATLVATLTLMGVATVLVGILPDRTSIGAWAGWG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LVVLRFFQGIGVGGEWGGAVLLSMESHQGDRRGFMASLPHIGVPLGLVVSVLAWSACSYW
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC
SGGNLEDGAWRIPFLASGALLVFGLVVRLGVPETPDFVKAQREVRASMPPLIEAIKTQWR
CCCCCCCCCEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
SILLAALIRPGEQASFYMLSTFVVLYGSAQLGLGREFMLNAVLLGAVASCFMVPYFGHLG
HHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIGRRRIYLTGAIVMTLFALPYFWLLDTRQPILVIVAILGLMAAHDMMYGPQAAYITES
HHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEECC
FPAHIRYTGASLGYQSASIIAGGPAPLVSLWLFNTFGTGYAVGAFLALLGLVSACGAFFL
CCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GREPLPEPARDQQLQPQEV
CCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVTDVSETASKRIERRAAIASVIGTTIEWYDFFIYGTAAALIFPKLYFGAGASEGLMESF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
STIFVGFLSRPIGAALFGHIGDRVGRKATLVATLTLMGVATVLVGILPDRTSIGAWAGWG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LVVLRFFQGIGVGGEWGGAVLLSMESHQGDRRGFMASLPHIGVPLGLVVSVLAWSACSYW
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCC
SGGNLEDGAWRIPFLASGALLVFGLVVRLGVPETPDFVKAQREVRASMPPLIEAIKTQWR
CCCCCCCCCEECCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
SILLAALIRPGEQASFYMLSTFVVLYGSAQLGLGREFMLNAVLLGAVASCFMVPYFGHLG
HHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
DRIGRRRIYLTGAIVMTLFALPYFWLLDTRQPILVIVAILGLMAAHDMMYGPQAAYITES
HHHCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEECC
FPAHIRYTGASLGYQSASIIAGGPAPLVSLWLFNTFGTGYAVGAFLALLGLVSACGAFFL
CCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GREPLPEPARDQQLQPQEV
CCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]