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The map label for this gene is holB [H]

Identifier: 182678511

GI number: 182678511

Start: 1739092

End: 1740210

Strand: Reverse

Name: holB [H]

Synonym: Bind_1535

Alternate gene names: 182678511

Gene position: 1740210-1739092 (Counterclockwise)

Preceding gene: 182678512

Following gene: 182678510

Centisome position: 41.73

GC content: 60.5

Gene sequence:

>1119_bases
ATGCCCGCTGAGACCGACCAGATCCTTGATTCCGATCTTTATGCTGGCACTAGGCATCCGCGTGAAACCTATGCCTTGAT
CGGGCATGAAGCGGCTGAAGCGAGCCTCCTTGCGGCCTATCGCGGCAATGTGCTGCCGCAGGCCATTCTTCTCGGCGGGC
CGATGGGGATCGGCAAAGCGACGCTGGCCTGGCGGCTCGCGCGCTTTCTCCTGGCGCATCCCGCCCCCCATGCGCCCGAG
GTCACAGCGGCCACCACGCTCCATGTGCCGCAAACCCATCCGATTTCCCATCAAATCGCTTCGCTCGCCCATCCCGATCT
GGTTCTGCTCCGGCGGGAATGGAACGAACGGGGCAAGAAATTTTTCAGCGAGATTCGCGTCGATGACGTGCGCCGGACGA
CGCATCTGTTCCAACAGGCGGCGGGGGCCGGCGGCTATCGGATCGCGATTATCGACAGCGTCGAGGATTTGAATGCTTCA
GGGGCCAATGCACTTCTGAAACTGATTGAGGAACCACCTCCCAGATCCTTGTTTTTACTGATTAGTCATCGACCCGGTCA
TGTCCTGCCGACGCTTCGCTCACGCTGCCGCCTCATGTCCTTGAAACCGCTATCCGACGAGGCGCTGGCGGCGATCATCG
CGGAACTCGGTCCGCCCTGGACCGATTCGGAATCGGCGCGGCATCAGGCGTCTCTTTTATCCCTGGCGCAGGCGGCGCAT
GGCTCGCTGCACCCGCTCTTACGCCGCCTCGACCCGAACGGGCGAGGGATCGAGGCAACCGTGAACCGTCTTTTGGGCGA
TCTCCCGCGTGTTGATTGGCGCGGCGTTCACGTCCTCGCGGACCAGATCGCCACCGATCCCGATAATAACCTCGAGATTT
TGCGGACCACGATCTTCGATTGGCTGGACGCGCGCCTACATGCCCAGACCCGCCAGATGGGTTCCGTCATGCCATTGGAT
CTCCACTCTCTTGCGCCTCTTGCGGAGGTATGGGACAAAGTGACGAGTGATCTGCGTGAGGCCGAGGCCCTCAATCTCGA
TAAGCGTCAGCTCATTCTTTCTCTTTTCACCGATTTGGCAGCGGCTGTAGAGGCTTCCACGCCTGTCTTACACCTTTAA

Upstream 100 bases:

>100_bases
AGGGAACCAGGCCGCTGCCTGCTAATCGACGCCGATGCCTCGGCGGACATCGTGACCGAACGTCTCTGGACCGCGATTCA
GCAAAGGCTGCTCCATCCCA

Downstream 100 bases:

>100_bases
TTCATGGCCTTTGTGACTGGAAAGCTTAAGGCAAGGATTATGCGTTAAGATTAGGATAAAGCGTATTTTCTTATTCGACC
GAATGAGATGATACATCGCT

Product: putative DNA polymerase III delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MPAETDQILDSDLYAGTRHPRETYALIGHEAAEASLLAAYRGNVLPQAILLGGPMGIGKATLAWRLARFLLAHPAPHAPE
VTAATTLHVPQTHPISHQIASLAHPDLVLLRREWNERGKKFFSEIRVDDVRRTTHLFQQAAGAGGYRIAIIDSVEDLNAS
GANALLKLIEEPPPRSLFLLISHRPGHVLPTLRSRCRLMSLKPLSDEALAAIIAELGPPWTDSESARHQASLLSLAQAAH
GSLHPLLRRLDPNGRGIEATVNRLLGDLPRVDWRGVHVLADQIATDPDNNLEILRTTIFDWLDARLHAQTRQMGSVMPLD
LHSLAPLAEVWDKVTSDLREAEALNLDKRQLILSLFTDLAAAVEASTPVLHL

Sequences:

>Translated_372_residues
MPAETDQILDSDLYAGTRHPRETYALIGHEAAEASLLAAYRGNVLPQAILLGGPMGIGKATLAWRLARFLLAHPAPHAPE
VTAATTLHVPQTHPISHQIASLAHPDLVLLRREWNERGKKFFSEIRVDDVRRTTHLFQQAAGAGGYRIAIIDSVEDLNAS
GANALLKLIEEPPPRSLFLLISHRPGHVLPTLRSRCRLMSLKPLSDEALAAIIAELGPPWTDSESARHQASLLSLAQAAH
GSLHPLLRRLDPNGRGIEATVNRLLGDLPRVDWRGVHVLADQIATDPDNNLEILRTTIFDWLDARLHAQTRQMGSVMPLD
LHSLAPLAEVWDKVTSDLREAEALNLDKRQLILSLFTDLAAAVEASTPVLHL
>Mature_371_residues
PAETDQILDSDLYAGTRHPRETYALIGHEAAEASLLAAYRGNVLPQAILLGGPMGIGKATLAWRLARFLLAHPAPHAPEV
TAATTLHVPQTHPISHQIASLAHPDLVLLRREWNERGKKFFSEIRVDDVRRTTHLFQQAAGAGGYRIAIIDSVEDLNASG
ANALLKLIEEPPPRSLFLLISHRPGHVLPTLRSRCRLMSLKPLSDEALAAIIAELGPPWTDSESARHQASLLSLAQAAHG
SLHPLLRRLDPNGRGIEATVNRLLGDLPRVDWRGVHVLADQIATDPDNNLEILRTTIFDWLDARLHAQTRQMGSVMPLDL
HSLAPLAEVWDKVTSDLREAEALNLDKRQLILSLFTDLAAAVEASTPVLHL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40810; Mature: 40679

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAETDQILDSDLYAGTRHPRETYALIGHEAAEASLLAAYRGNVLPQAILLGGPMGIGKA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHH
TLAWRLARFLLAHPAPHAPEVTAATTLHVPQTHPISHQIASLAHPDLVLLRREWNERGKK
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
FFSEIRVDDVRRTTHLFQQAAGAGGYRIAIIDSVEDLNASGANALLKLIEEPPPRSLFLL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCHHHHHHHHCCCCCCEEEEE
ISHRPGHVLPTLRSRCRLMSLKPLSDEALAAIIAELGPPWTDSESARHQASLLSLAQAAH
EECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GSLHPLLRRLDPNGRGIEATVNRLLGDLPRVDWRGVHVLADQIATDPDNNLEILRTTIFD
CCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
WLDARLHAQTRQMGSVMPLDLHSLAPLAEVWDKVTSDLREAEALNLDKRQLILSLFTDLA
HHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AAVEASTPVLHL
HHHHCCCCCCCC
>Mature Secondary Structure 
PAETDQILDSDLYAGTRHPRETYALIGHEAAEASLLAAYRGNVLPQAILLGGPMGIGKA
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHH
TLAWRLARFLLAHPAPHAPEVTAATTLHVPQTHPISHQIASLAHPDLVLLRREWNERGKK
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
FFSEIRVDDVRRTTHLFQQAAGAGGYRIAIIDSVEDLNASGANALLKLIEEPPPRSLFLL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHCCCCCHHHHHHHHCCCCCCEEEEE
ISHRPGHVLPTLRSRCRLMSLKPLSDEALAAIIAELGPPWTDSESARHQASLLSLAQAAH
EECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHC
GSLHPLLRRLDPNGRGIEATVNRLLGDLPRVDWRGVHVLADQIATDPDNNLEILRTTIFD
CCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
WLDARLHAQTRQMGSVMPLDLHSLAPLAEVWDKVTSDLREAEALNLDKRQLILSLFTDLA
HHHHHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
AAVEASTPVLHL
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]