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The map label for this gene is metG [H]

Identifier: 182678510

GI number: 182678510

Start: 1737398

End: 1738990

Strand: Reverse

Name: metG [H]

Synonym: Bind_1534

Alternate gene names: 182678510

Gene position: 1738990-1737398 (Counterclockwise)

Preceding gene: 182678511

Following gene: 182678509

Centisome position: 41.7

GC content: 58.51

Gene sequence:

>1593_bases
GTGTTCGACCAGCCGGATCGGGACAAACTCAAGATCATGACCATGGCCGGCCGCCCCCGCTTCTATCTCACGACCGCCAT
TCCCTATGCCAATGGCGCTCCCCATATCGGCCATGCCTATGAGCGGCTCGCCTGCGACGCGATCACCCGTTTCAAGCGGC
TCGATGGCTATGACGTGCTGTTCGTGACCGGCATGGACGAGCACGGCCAAAAAATGCAGCAGACGGCCGCCCGCGACCAT
ATCACACCGCAGGAACTGGCCGACCGCACGGCCGCTCAATTCGCTGCTATGGGGCTCGCGCTTGACGCACCGACCGATGA
TGTCGTGCGCACGACGGAGCCACGCCATCATGTAGCGGCGCAAGAGATCTGGAACCGTATGGCCGCCAATGGCGATATTT
ATCTCTCCAAATATCCCGGCTGGTATTCGGTCCGCGATGAGGCTTTCTTCGACGAGGGTGAACTGACCGAGGGGCCCAAT
GGCACCAAGCTCGCTCCAACCGGCGCGCCGGTCGAATGGGTCGAGGAAGAGAGCTATTATTTCAAGCTTTCAGCCTATCA
AGAGCGCTTGCTCGCGCATTATCAGGCCCATCCGGATTTCATCACACCCGAGAAATATCGCAACGAGATCGTCTCTTTCG
TCGAGCGCGGCTTGACCGATCTTTCGATCAGCCGCTCGACCTTCGACTGGGGCATTCCCGTGCCGGGCGATGCCAAACAT
GTCATGTATGTCTGGGTCGATGCCCTGACCAATTATATTACCGCCACGGGTTTCCCTGATGCGGAGGCGCCGCGCGCGGT
TTTCTGGCCGGCCGATGCGCATGTGATCGGCAAGGATATCACCCGCTTCCACGCGATTTACTGGCCGGCGTTCCTGATGT
CGGCTGGAATTGCTCTGCCAAAACAGATCGTCGTGCATGGATTTCTGTTCAACCGCGGCGAGAAAATGTCGAAATCAGTG
GGCAATGTCATCGACCCGCTGACTTTGATCGAGACTTATGGCCTCGACCCATTCCGCTATTTCTTCCTCCGCGAAGTGCC
GTTCGGCCAGGATGGCAATTATTCGCATGAAGCGATCGTCAACCGCACCAATGCCGATCTCGCCAATGACCTTGGCAATC
TGGCGCAGCGCTCGCTCTCCATGCTCGCCAAAAATTGCGGGGGTAAGGTGCCGCAGCCCGGCGCCTTCAGCGAGGCCGAC
AAAGCGATCCTCGACCTCTCCCGCGCCCTGGTCGAAAAGAGCCGGGATCTGATGCAGGATTTCTCGCTGCATCTTATCTT
AGGCGAAATCTGGCGCGTCGTTGGCGAGGCCAATCGCTATTTCGCGGGCGAAGAGCCGTGGATCAAGCGCAAGACCGATC
CCGCTCGCATGGAGACCATTCTCTATGTGACAGCAGAAGTGTTGCGGCGGGTCGCTATTCTGACTCAGCCTTTCATTCCG
CAATCGGCCTCGAAATTGCTCGATTCTCTGGGTGTCGCGGCGGAAGCTCGCCTTTTCATCCATGTGGAAGAAGGCCCTGA
TCTGCCCCCCGGCACGGTTTTGCCGACACCTGTGCCGATCTTTCCCCGTATCGAAACGGAAGCCAAGTCATAG

Upstream 100 bases:

>100_bases
TCATGGCCTTTGTGACTGGAAAGCTTAAGGCAAGGATTATGCGTTAAGATTAGGATAAAGCGTATTTTCTTATTCGACCG
AATGAGATGATACATCGCTG

Downstream 100 bases:

>100_bases
ACATGCTGATCGATACCCATTGCCATCTTGATTTTCCCGATTTCGCGGCCGAGCGCGACGAGATTGTCGGCCGTGCGCGG
GCGGCGGGCCTTGGGCGTAT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MFDQPDRDKLKIMTMAGRPRFYLTTAIPYANGAPHIGHAYERLACDAITRFKRLDGYDVLFVTGMDEHGQKMQQTAARDH
ITPQELADRTAAQFAAMGLALDAPTDDVVRTTEPRHHVAAQEIWNRMAANGDIYLSKYPGWYSVRDEAFFDEGELTEGPN
GTKLAPTGAPVEWVEEESYYFKLSAYQERLLAHYQAHPDFITPEKYRNEIVSFVERGLTDLSISRSTFDWGIPVPGDAKH
VMYVWVDALTNYITATGFPDAEAPRAVFWPADAHVIGKDITRFHAIYWPAFLMSAGIALPKQIVVHGFLFNRGEKMSKSV
GNVIDPLTLIETYGLDPFRYFFLREVPFGQDGNYSHEAIVNRTNADLANDLGNLAQRSLSMLAKNCGGKVPQPGAFSEAD
KAILDLSRALVEKSRDLMQDFSLHLILGEIWRVVGEANRYFAGEEPWIKRKTDPARMETILYVTAEVLRRVAILTQPFIP
QSASKLLDSLGVAAEARLFIHVEEGPDLPPGTVLPTPVPIFPRIETEAKS

Sequences:

>Translated_530_residues
MFDQPDRDKLKIMTMAGRPRFYLTTAIPYANGAPHIGHAYERLACDAITRFKRLDGYDVLFVTGMDEHGQKMQQTAARDH
ITPQELADRTAAQFAAMGLALDAPTDDVVRTTEPRHHVAAQEIWNRMAANGDIYLSKYPGWYSVRDEAFFDEGELTEGPN
GTKLAPTGAPVEWVEEESYYFKLSAYQERLLAHYQAHPDFITPEKYRNEIVSFVERGLTDLSISRSTFDWGIPVPGDAKH
VMYVWVDALTNYITATGFPDAEAPRAVFWPADAHVIGKDITRFHAIYWPAFLMSAGIALPKQIVVHGFLFNRGEKMSKSV
GNVIDPLTLIETYGLDPFRYFFLREVPFGQDGNYSHEAIVNRTNADLANDLGNLAQRSLSMLAKNCGGKVPQPGAFSEAD
KAILDLSRALVEKSRDLMQDFSLHLILGEIWRVVGEANRYFAGEEPWIKRKTDPARMETILYVTAEVLRRVAILTQPFIP
QSASKLLDSLGVAAEARLFIHVEEGPDLPPGTVLPTPVPIFPRIETEAKS
>Mature_530_residues
MFDQPDRDKLKIMTMAGRPRFYLTTAIPYANGAPHIGHAYERLACDAITRFKRLDGYDVLFVTGMDEHGQKMQQTAARDH
ITPQELADRTAAQFAAMGLALDAPTDDVVRTTEPRHHVAAQEIWNRMAANGDIYLSKYPGWYSVRDEAFFDEGELTEGPN
GTKLAPTGAPVEWVEEESYYFKLSAYQERLLAHYQAHPDFITPEKYRNEIVSFVERGLTDLSISRSTFDWGIPVPGDAKH
VMYVWVDALTNYITATGFPDAEAPRAVFWPADAHVIGKDITRFHAIYWPAFLMSAGIALPKQIVVHGFLFNRGEKMSKSV
GNVIDPLTLIETYGLDPFRYFFLREVPFGQDGNYSHEAIVNRTNADLANDLGNLAQRSLSMLAKNCGGKVPQPGAFSEAD
KAILDLSRALVEKSRDLMQDFSLHLILGEIWRVVGEANRYFAGEEPWIKRKTDPARMETILYVTAEVLRRVAILTQPFIP
QSASKLLDSLGVAAEARLFIHVEEGPDLPPGTVLPTPVPIFPRIETEAKS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=505, Percent_Identity=39.4059405940594, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI14043022, Length=560, Percent_Identity=23.3928571428571, Blast_Score=140, Evalue=3e-33,
Organism=Escherichia coli, GI1788432, Length=508, Percent_Identity=25.7874015748031, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI212645096, Length=497, Percent_Identity=36.4185110663984, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI71992781, Length=391, Percent_Identity=35.5498721227622, Blast_Score=254, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17540794, Length=531, Percent_Identity=23.728813559322, Blast_Score=132, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=32.3529411764706, Blast_Score=287, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6321704, Length=569, Percent_Identity=22.4956063268893, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI21358483, Length=509, Percent_Identity=37.1316306483301, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI24655311, Length=575, Percent_Identity=26.0869565217391, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 59408; Mature: 59408

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDQPDRDKLKIMTMAGRPRFYLTTAIPYANGAPHIGHAYERLACDAITRFKRLDGYDVL
CCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
FVTGMDEHGQKMQQTAARDHITPQELADRTAAQFAAMGLALDAPTDDVVRTTEPRHHVAA
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHCCCCCHHHHHH
QEIWNRMAANGDIYLSKYPGWYSVRDEAFFDEGELTEGPNGTKLAPTGAPVEWVEEESYY
HHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCHHEECCCCEE
FKLSAYQERLLAHYQAHPDFITPEKYRNEIVSFVERGLTDLSISRSTFDWGIPVPGDAKH
EEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCE
VMYVWVDALTNYITATGFPDAEAPRAVFWPADAHVIGKDITRFHAIYWPAFLMSAGIALP
EHHHHHHHHHHHHEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KQIVVHGFLFNRGEKMSKSVGNVIDPLTLIETYGLDPFRYFFLREVPFGQDGNYSHEAIV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHH
NRTNADLANDLGNLAQRSLSMLAKNCGGKVPQPGAFSEADKAILDLSRALVEKSRDLMQD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FSLHLILGEIWRVVGEANRYFAGEEPWIKRKTDPARMETILYVTAEVLRRVAILTQPFIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
QSASKLLDSLGVAAEARLFIHVEEGPDLPPGTVLPTPVPIFPRIETEAKS
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFDQPDRDKLKIMTMAGRPRFYLTTAIPYANGAPHIGHAYERLACDAITRFKRLDGYDVL
CCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEE
FVTGMDEHGQKMQQTAARDHITPQELADRTAAQFAAMGLALDAPTDDVVRTTEPRHHVAA
EEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHCCCCCHHHHHH
QEIWNRMAANGDIYLSKYPGWYSVRDEAFFDEGELTEGPNGTKLAPTGAPVEWVEEESYY
HHHHHHHHCCCCEEEECCCCCEECCCCCCCCCCCCCCCCCCCEECCCCCCHHEECCCCEE
FKLSAYQERLLAHYQAHPDFITPEKYRNEIVSFVERGLTDLSISRSTFDWGIPVPGDAKH
EEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCE
VMYVWVDALTNYITATGFPDAEAPRAVFWPADAHVIGKDITRFHAIYWPAFLMSAGIALP
EHHHHHHHHHHHHEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KQIVVHGFLFNRGEKMSKSVGNVIDPLTLIETYGLDPFRYFFLREVPFGQDGNYSHEAIV
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCHHHHH
NRTNADLANDLGNLAQRSLSMLAKNCGGKVPQPGAFSEADKAILDLSRALVEKSRDLMQD
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
FSLHLILGEIWRVVGEANRYFAGEEPWIKRKTDPARMETILYVTAEVLRRVAILTQPFIP
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
QSASKLLDSLGVAAEARLFIHVEEGPDLPPGTVLPTPVPIFPRIETEAKS
HHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]