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The map label for this gene is tmk [H]

Identifier: 182678512

GI number: 182678512

Start: 1740200

End: 1740913

Strand: Reverse

Name: tmk [H]

Synonym: Bind_1536

Alternate gene names: 182678512

Gene position: 1740913-1740200 (Counterclockwise)

Preceding gene: 182678513

Following gene: 182678511

Centisome position: 41.75

GC content: 61.34

Gene sequence:

>714_bases
ATGAGTATTCCTTCCGAGCGTGCCGCTTCCGAACACATTGTGGAAAATCCCGTCCCCACACGCGGATTCTTCATCACTCT
GGAAGGGGGAGAAGGAACCGGAAAATCGACGCAGGCGCGCAAGCTTGTGGCAAGATTCGAGGCGCAAGGTCTTCGTGCTA
TCGCCACACGCGAGCCTGGAGGTTCTCCAAATGCCGAAATTCTGCGCGATATTCTCCTTTCTGGGGTCATCAAGCCGCTC
GGTCCGCTGGCCGAGGCTCTGATGTTTTCCGCCGCGCGGATCGATCATCTCGATCAAGTCATCAAGCCCGCGCTCGCGGC
GGGCACGACAGTCGTCAGCGACCGTTTTGCCGATTCGACGCGGGTCTATCAGGGCGTGGCGGGGAATCTCGATCCGCACC
GGCTCACCCTTCTCGAACAAGCGGTCCTGAACGGCACAGTCCCCGATCTGACGCTCGTCCTCGACTTGGCGCCAGAAACC
GGCCTGGCGCGCGCCGCCGCACGGCGCGCCAATATTGATTCCGGCGATGGTCCCGATCGTTTCGAAAGCGAGGATCTTAC
CTATCATCGGCGCTTGCGGGAGGCCTTCCGGGCACTCCCTAAGAGGGAACCAGGCCGCTGCCTGCTAATCGACGCCGATG
CCTCGGCGGACATCGTGACCGAACGTCTCTGGACCGCGATTCAGCAAAGGCTGCTCCATCCCAATGCCCGCTGA

Upstream 100 bases:

>100_bases
ATACCGCCGAAGCGGTTCCACAGGGCCCCTTGCCGCGCCGGGCACTCGATGCCGGATATGAAGCCGGCGTGCTGTTGTGG
CGCAAATATATGACCAAGCC

Downstream 100 bases:

>100_bases
GACCGACCAGATCCTTGATTCCGATCTTTATGCTGGCACTAGGCATCCGCGTGAAACCTATGCCTTGATCGGGCATGAAG
CGGCTGAAGCGAGCCTCCTT

Product: dTMP kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSIPSERAASEHIVENPVPTRGFFITLEGGEGTGKSTQARKLVARFEAQGLRAIATREPGGSPNAEILRDILLSGVIKPL
GPLAEALMFSAARIDHLDQVIKPALAAGTTVVSDRFADSTRVYQGVAGNLDPHRLTLLEQAVLNGTVPDLTLVLDLAPET
GLARAAARRANIDSGDGPDRFESEDLTYHRRLREAFRALPKREPGRCLLIDADASADIVTERLWTAIQQRLLHPNAR

Sequences:

>Translated_237_residues
MSIPSERAASEHIVENPVPTRGFFITLEGGEGTGKSTQARKLVARFEAQGLRAIATREPGGSPNAEILRDILLSGVIKPL
GPLAEALMFSAARIDHLDQVIKPALAAGTTVVSDRFADSTRVYQGVAGNLDPHRLTLLEQAVLNGTVPDLTLVLDLAPET
GLARAAARRANIDSGDGPDRFESEDLTYHRRLREAFRALPKREPGRCLLIDADASADIVTERLWTAIQQRLLHPNAR
>Mature_236_residues
SIPSERAASEHIVENPVPTRGFFITLEGGEGTGKSTQARKLVARFEAQGLRAIATREPGGSPNAEILRDILLSGVIKPLG
PLAEALMFSAARIDHLDQVIKPALAAGTTVVSDRFADSTRVYQGVAGNLDPHRLTLLEQAVLNGTVPDLTLVLDLAPETG
LARAAARRANIDSGDGPDRFESEDLTYHRRLREAFRALPKREPGRCLLIDADASADIVTERLWTAIQQRLLHPNAR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=190, Percent_Identity=43.1578947368421, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 25666; Mature: 25534

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPSERAASEHIVENPVPTRGFFITLEGGEGTGKSTQARKLVARFEAQGLRAIATREPG
CCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCC
GSPNAEILRDILLSGVIKPLGPLAEALMFSAARIDHLDQVIKPALAAGTTVVSDRFADST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
RVYQGVAGNLDPHRLTLLEQAVLNGTVPDLTLVLDLAPETGLARAAARRANIDSGDGPDR
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
FESEDLTYHRRLREAFRALPKREPGRCLLIDADASADIVTERLWTAIQQRLLHPNAR
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIPSERAASEHIVENPVPTRGFFITLEGGEGTGKSTQARKLVARFEAQGLRAIATREPG
CCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEECCCCC
GSPNAEILRDILLSGVIKPLGPLAEALMFSAARIDHLDQVIKPALAAGTTVVSDRFADST
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHH
RVYQGVAGNLDPHRLTLLEQAVLNGTVPDLTLVLDLAPETGLARAAARRANIDSGDGPDR
HHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
FESEDLTYHRRLREAFRALPKREPGRCLLIDADASADIVTERLWTAIQQRLLHPNAR
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]