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The map label for this gene is miaB

Identifier: 182678041

GI number: 182678041

Start: 1211013

End: 1212545

Strand: Direct

Name: miaB

Synonym: Bind_1055

Alternate gene names: 182678041

Gene position: 1211013-1212545 (Clockwise)

Preceding gene: 182678040

Following gene: 182678042

Centisome position: 29.04

GC content: 58.32

Gene sequence:

>1533_bases
ATGTCTGGCTTTAATGATTCGCCGGTGCCTGAATCCGCCGAAAAGGCAGACGGGCTTCTATCGCAAGAGCGCGGTACGCA
AGATCCATTGGTGAACACGCGAAAACTTTTCGTCAAATCCTATGGCTGTCAGATGAACGTCTATGACGCGGAGCGCATGG
CCGATCTTCTGGCACCTGAAGGCTATGCCGAAACCAGCGCGCCGGAGGATGCTGATCTCGTCATCCTCAATACTTGCCAT
ATTCGCGAGCATGCAGCGGAAAAAGTCTTTTCTGAACTCGGCAAATTGCGCCTTCTCAAGGCCGAGCAACAAGCCGCCGG
GCGGCCGGTCAAGATCGTTGTCGCGGGTTGTGTTGCTCAGGCAGAGGGTGAGGAAATCCTGCGCCGGCAAAAGGCGGTGG
ATCTCGTCGTCGGGCCACAGAGCTATCACCGCTTGCCCGATCTTCTGCGCCGCGTGGCGCATACACCGGGCCTGGTCGAC
ACCGAATTTCCCGCCGAGGACAAATTCGATCATCTTGTGGCCCCCCAGCCTGAAAAGATCGCCGAGCGCGGAGTCGGCGC
TTTCGTAACCGTGCAGGAAGGGTGCGATAAATTCTGCAGCTTCTGCGTCGTGCCCTATACGAGGGGCGCCGAGACATCGC
GTCCGGTGGAAGCGATTTTGGCCGAGGTTGAGACCCTGATCGCTTCTGGCGTTCGCGAGGTGACGCTGATCGGGCAAAAC
GTCAATGCCTATCACGGATTTGATGCTATGACCGGCGCGCCGGCTTCGCTCGCGAGCCTCATGGCCCGCGTCGCCGCCAT
GCCAGGACTCTTGCGCATTCGGTATACGACCAGCCATCCCAATGACATGGGCGAGGATCTGATCGCCGCGCATCGCGATA
TTCCGGCGCTCATGCCTTTCCTGCATCTGCCGGTGCAATCCGGGTCGGACAAGATCCTCGCGGCGATGAATCGCCGCCAT
AAAGCCGGTGATTATCTCGAACTCATCGCCTCGATCCGCGCAGCGCGTCCCGACATTGCACTTTCTTCGGATTTTATCGT
CGGCTTTCCCGGCGAGACGGAGGCTGATTTCGAGGCGACGCTCGCGCTGATTGAACAGGTCGGTTTCGCCAGCGCTTTTT
CGTTCAAATATTCACAACGCCCAGGAACGCCCGGCGCCGATCGGCCAGATCAAATCGACGAGGATGTAAAAGCTCAAAGG
CTCGCCAGGCTTCAGGCCTTGCTCGAAGAGCAAAGACAGGCATTCAACAAGGCTATGATCGGACGTGTCCTGCCAGTGCT
GTTTGAAAAGCCCGGCCGGCATCCGGGGCAGATCGCGGGCAAGACGCCTTATCTTCAAGCTCTGTATGCGGAAGGTGACA
AAGCTTTGATCGGAACGGTGCAACCAGTTGAAATTCTGGAAGCTGGCCCCAATTCCTTCCATGGCCGCCTTCTTGCGCGG
GAGACAGGCCAGGAATCGGCACAAGGGCAGGAATCGGCACAAGGGATGGAGCGAATGGAGCAAAATGCTCGGGCTTGGGA
GGTGCCGGTTTGA

Upstream 100 bases:

>100_bases
CCTCTGGATGAGGCAAAGCAGTGAGGCCTGAGAGAAAGGCCTAGGGCAAAAGCGCGTGGAATATAAAGGTAAGGCCGAGC
CCTCGGCGATTGCGGGGATG

Downstream 100 bases:

>100_bases
GATTCTCCGACCGATCCGCTTCTCTCCCACGCGGCGAGGCCAAGCGGCAAATGCCGGCCCTTCCTTCAGGAGAGCCGACG
GCCGAGATGACCCTCGCCTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPEGYAETSAPEDADLVILNTCH
IREHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQAEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVD
TEFPAEDKFDHLVAPQPEKIAERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQN
VNAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPFLHLPVQSGSDKILAAMNRRH
KAGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEATLALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQR
LARLQALLEEQRQAFNKAMIGRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLAR
ETGQESAQGQESAQGMERMEQNARAWEVPV

Sequences:

>Translated_510_residues
MSGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPEGYAETSAPEDADLVILNTCH
IREHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQAEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVD
TEFPAEDKFDHLVAPQPEKIAERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQN
VNAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPFLHLPVQSGSDKILAAMNRRH
KAGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEATLALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQR
LARLQALLEEQRQAFNKAMIGRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLAR
ETGQESAQGQESAQGMERMEQNARAWEVPV
>Mature_509_residues
SGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPEGYAETSAPEDADLVILNTCHI
REHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQAEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVDT
EFPAEDKFDHLVAPQPEKIAERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQNV
NAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPFLHLPVQSGSDKILAAMNRRHK
AGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEATLALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQRL
ARLQALLEEQRQAFNKAMIGRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLARE
TGQESAQGQESAQGMERMEQNARAWEVPV

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=488, Percent_Identity=32.9918032786885, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI28872784, Length=494, Percent_Identity=33.1983805668016, Blast_Score=276, Evalue=4e-74,
Organism=Homo sapiens, GI93277076, Length=447, Percent_Identity=27.5167785234899, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1786882, Length=478, Percent_Identity=47.2803347280335, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1787057, Length=403, Percent_Identity=30.272952853598, Blast_Score=185, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17553146, Length=422, Percent_Identity=35.3080568720379, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI71996771, Length=223, Percent_Identity=29.5964125560538, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI21356207, Length=436, Percent_Identity=35.0917431192661, Blast_Score=275, Evalue=4e-74,
Organism=Drosophila melanogaster, GI19922432, Length=486, Percent_Identity=29.6296296296296, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_BEII9 (B2IIK5)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832187.1
- GeneID:   6198171
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1055
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 55496; Mature: 55364

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPE
CCCCCCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCC
GYAETSAPEDADLVILNTCHIREHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQ
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
AEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVDTEFPAEDKFDHLVAPQPEKI
CCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
AERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQN
HHCCCCEEEEHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
VNAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPF
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHH
LHLPVQSGSDKILAAMNRRHKAGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEAT
HHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCHHHHH
LALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQRLARLQALLEEQRQAFNKAMI
HHHHHHHCCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLAR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHCCCCCHHCEEHHH
ETGQESAQGQESAQGMERMEQNARAWEVPV
HCCCHHHCHHHHHHHHHHHHHCCCEECCCC
>Mature Secondary Structure 
SGFNDSPVPESAEKADGLLSQERGTQDPLVNTRKLFVKSYGCQMNVYDAERMADLLAPE
CCCCCCCCCCCHHHHHCHHHHCCCCCCCCHHHHHHHHHHCCCEEECCCHHHHHHHHCCC
GYAETSAPEDADLVILNTCHIREHAAEKVFSELGKLRLLKAEQQAAGRPVKIVVAGCVAQ
CCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHH
AEGEEILRRQKAVDLVVGPQSYHRLPDLLRRVAHTPGLVDTEFPAEDKFDHLVAPQPEKI
CCHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
AERGVGAFVTVQEGCDKFCSFCVVPYTRGAETSRPVEAILAEVETLIASGVREVTLIGQN
HHCCCCEEEEHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCC
VNAYHGFDAMTGAPASLASLMARVAAMPGLLRIRYTTSHPNDMGEDLIAAHRDIPALMPF
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCHHHHHH
LHLPVQSGSDKILAAMNRRHKAGDYLELIASIRAARPDIALSSDFIVGFPGETEADFEAT
HHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEECCCEEEECCCCCCCHHHHH
LALIEQVGFASAFSFKYSQRPGTPGADRPDQIDEDVKAQRLARLQALLEEQRQAFNKAMI
HHHHHHHCCHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVLPVLFEKPGRHPGQIAGKTPYLQALYAEGDKALIGTVQPVEILEAGPNSFHGRLLAR
HHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCHHHHHHCCCCCHHCEEHHH
ETGQESAQGQESAQGMERMEQNARAWEVPV
HCCCHHHCHHHHHHHHHHHHHCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA