Click here to switch to the map view.

The map label for this gene is pyrG

Identifier: 197294670

GI number: 197294670

Start: 623179

End: 624822

Strand: Direct

Name: pyrG

Synonym: PAa_0628

Alternate gene names: 197294670

Gene position: 623179-624822 (Clockwise)

Preceding gene: 197294669

Following gene: 197294675

Centisome position: 70.82

GC content: 29.87

Gene sequence:

>1644_bases
TTGAATAACAAAAAATTAAAATCAAAATTTATTTTTATTACTGGAGGAGTCGTTTCGAGTTTAGGAAAAGGAATCACTGC
TGCTTCTATTGGAAATATTTTAAAAAATCGTGGTTTCAAAGTTAGTATTCAAAAATTAGATCCGTATATAAATATTGATC
CAGGAACTATGAGTCCTTACCAACATGGCGAAGTTTTTGTCACCAACGATGGTGCAGAAACCGATCTTGATTTAGGACAT
TATGAAAGATTTTTAGATGAAAATATGTCTAAAAGTTCTAATATTACTGCAGGACAAATTTATCAAAGCGTCATCAACAA
AGAAAGAGAAGGAAAATACTTAGGAAAAACCGTACAAGTAATTCCTCACATTACCGAAGAAATTAAAAAAAAAATAATCA
AGGCAGCTATAGTTCATCAATCTGATATCGTAATTGTAGAAATTGGTGGAACTGTTGGTGATATAGAATCGGCACCTTTT
TTGGAAGCTATTAGACAATTTCGTTATGAAGTTGGTTATCATAACGTTTTGTATTTGCACACTACTTTAGTTCCTTATTT
AAAAAAAGCCCAAGAAATAAAAACCAAACCAACTCAACATAGTGTCAAAGAATTACGAGCTTTGGGAATACAACCTCAAA
TTTTAGTTTTAAGAAGTGAAATTTCTATCAACAAAGAAATTAAAAATAAAATAGCTTCTTTGTGTGATATCAACCCTCAA
GCGATTTTTGAAGCTTTAGATGTAGATATTTTATATCAAATTATTTTAAATTTATTTGAACAAAGGATTGACCACTTTAT
TTTAAATCATTTTCAATTGCCAAACAATATTCAAATCGATTTAAAAGATTGGCGTTCATTAATCAATTGCATTCAAAACT
TAAAAGAAAAAGTAGTAATTGGTTTGGTTGGCAAATACGTTATCTTGCACGATGCTTATTTATCTATCGTTGAATCCTTA
AAACATGCTGCTTATTATTACAATTCTGATATTGAAATTAAATGGATAGATTCTGAAAAATTAAATTCAACCAATGTCAA
AACTTTATTAAATGATTGTGATGGCATTTTGGTGCCGCATGGTTTTGGACCTAGAGCCATTGAGGGAAAAATTTTGGCTA
TCCAATACGCACGTGAACAAAAGATTCCTTTTTTTGGGATTTGTTTTGGAATGCAGTTAGCAGTTGTTGAATACGCAAGA
AACGTATTGTTTTTGGATGGCGCTAACTCTACTGAAATTGATGAAAAAACTCCTCATCCAGTTATTACTAAAAAAATAAA
AAATAGTAATTTAGGGGGGACTTTAAGATTAGGTTCTTATCGTTGTTGCCTTCAAAAAAATAGTAAAAGTGAAGCGATTT
ATCAACAAACAACTATTTATGAAAGACATCGTCATCGTTTCGAAATGAACCCTTCTTATGTCTCTTTGTTTGAAAAAAAT
AATGATTTCTTAGTTTCTGGTTTCAACCCTGAAAAAAATTTGGCAGAAATAATTGAATTAAAAAACCATCCTTGGTTTAT
GGCGGTTCAATTTCATCCTGAATTTTTATCAAGACCATTAAAACCTCATCCTTTATTTAAAAGTTTTGTTGAGGCAGCTT
TATTAAAAAACGGAAAAAATATCAAAAAATCTAAATTGTCATAA

Upstream 100 bases:

>100_bases
ACAATTGGAAAAAAAATAAGCGGGTAATTTTTTTGTTTTTTTAACCTTCAATATTAATTTTAATATCTTATTAAAAAAAC
TAAAGAAAGGACTCAAACAC

Downstream 100 bases:

>100_bases
ACGTTCTAAAGAACCTTAAAAATTTAATCCTTTATTTAAAAATATTTTTGAAATATCCAATCAAAACAACTAAAAAAATA
TCAAAAAAAGCTAAATTGTC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MNNKKLKSKFIFITGGVVSSLGKGITAASIGNILKNRGFKVSIQKLDPYINIDPGTMSPYQHGEVFVTNDGAETDLDLGH
YERFLDENMSKSSNITAGQIYQSVINKEREGKYLGKTVQVIPHITEEIKKKIIKAAIVHQSDIVIVEIGGTVGDIESAPF
LEAIRQFRYEVGYHNVLYLHTTLVPYLKKAQEIKTKPTQHSVKELRALGIQPQILVLRSEISINKEIKNKIASLCDINPQ
AIFEALDVDILYQIILNLFEQRIDHFILNHFQLPNNIQIDLKDWRSLINCIQNLKEKVVIGLVGKYVILHDAYLSIVESL
KHAAYYYNSDIEIKWIDSEKLNSTNVKTLLNDCDGILVPHGFGPRAIEGKILAIQYAREQKIPFFGICFGMQLAVVEYAR
NVLFLDGANSTEIDEKTPHPVITKKIKNSNLGGTLRLGSYRCCLQKNSKSEAIYQQTTIYERHRHRFEMNPSYVSLFEKN
NDFLVSGFNPEKNLAEIIELKNHPWFMAVQFHPEFLSRPLKPHPLFKSFVEAALLKNGKNIKKSKLS

Sequences:

>Translated_547_residues
MNNKKLKSKFIFITGGVVSSLGKGITAASIGNILKNRGFKVSIQKLDPYINIDPGTMSPYQHGEVFVTNDGAETDLDLGH
YERFLDENMSKSSNITAGQIYQSVINKEREGKYLGKTVQVIPHITEEIKKKIIKAAIVHQSDIVIVEIGGTVGDIESAPF
LEAIRQFRYEVGYHNVLYLHTTLVPYLKKAQEIKTKPTQHSVKELRALGIQPQILVLRSEISINKEIKNKIASLCDINPQ
AIFEALDVDILYQIILNLFEQRIDHFILNHFQLPNNIQIDLKDWRSLINCIQNLKEKVVIGLVGKYVILHDAYLSIVESL
KHAAYYYNSDIEIKWIDSEKLNSTNVKTLLNDCDGILVPHGFGPRAIEGKILAIQYAREQKIPFFGICFGMQLAVVEYAR
NVLFLDGANSTEIDEKTPHPVITKKIKNSNLGGTLRLGSYRCCLQKNSKSEAIYQQTTIYERHRHRFEMNPSYVSLFEKN
NDFLVSGFNPEKNLAEIIELKNHPWFMAVQFHPEFLSRPLKPHPLFKSFVEAALLKNGKNIKKSKLS
>Mature_547_residues
MNNKKLKSKFIFITGGVVSSLGKGITAASIGNILKNRGFKVSIQKLDPYINIDPGTMSPYQHGEVFVTNDGAETDLDLGH
YERFLDENMSKSSNITAGQIYQSVINKEREGKYLGKTVQVIPHITEEIKKKIIKAAIVHQSDIVIVEIGGTVGDIESAPF
LEAIRQFRYEVGYHNVLYLHTTLVPYLKKAQEIKTKPTQHSVKELRALGIQPQILVLRSEISINKEIKNKIASLCDINPQ
AIFEALDVDILYQIILNLFEQRIDHFILNHFQLPNNIQIDLKDWRSLINCIQNLKEKVVIGLVGKYVILHDAYLSIVESL
KHAAYYYNSDIEIKWIDSEKLNSTNVKTLLNDCDGILVPHGFGPRAIEGKILAIQYAREQKIPFFGICFGMQLAVVEYAR
NVLFLDGANSTEIDEKTPHPVITKKIKNSNLGGTLRLGSYRCCLQKNSKSEAIYQQTTIYERHRHRFEMNPSYVSLFEKN
NDFLVSGFNPEKNLAEIIELKNHPWFMAVQFHPEFLSRPLKPHPLFKSFVEAALLKNGKNIKKSKLS

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=548, Percent_Identity=45.985401459854, Blast_Score=487, Evalue=1e-138,
Organism=Homo sapiens, GI28559085, Length=547, Percent_Identity=46.617915904936, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI28559083, Length=547, Percent_Identity=46.617915904936, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI221316689, Length=547, Percent_Identity=46.617915904936, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI1789142, Length=541, Percent_Identity=49.9075785582255, Blast_Score=520, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI25148299, Length=608, Percent_Identity=36.6776315789474, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6322563, Length=561, Percent_Identity=40.6417112299465, Blast_Score=438, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6319432, Length=559, Percent_Identity=40.4293381037567, Blast_Score=435, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24664469, Length=563, Percent_Identity=44.0497335701599, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=507, Percent_Identity=42.603550295858, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_PHYAS (B1VAI9)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799211.1
- ProteinModelPortal:   B1VAI9
- SMR:   B1VAI9
- GeneID:   6798923
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG597806
- OMA:   NIKIDPY
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 62165; Mature: 62165

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 388-388 ACT_SITE 512-512 ACT_SITE 514-514

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKKLKSKFIFITGGVVSSLGKGITAASIGNILKNRGFKVSIQKLDPYINIDPGTMSPY
CCCCEEECEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCC
QHGEVFVTNDGAETDLDLGHYERFLDENMSKSSNITAGQIYQSVINKEREGKYLGKTVQV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
IPHITEEIKKKIIKAAIVHQSDIVIVEIGGTVGDIESAPFLEAIRQFRYEVGYHNVLYLH
HHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHH
TTLVPYLKKAQEIKTKPTQHSVKELRALGIQPQILVLRSEISINKEIKNKIASLCDINPQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHH
AIFEALDVDILYQIILNLFEQRIDHFILNHFQLPNNIQIDLKDWRSLINCIQNLKEKVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GLVGKYVILHDAYLSIVESLKHAAYYYNSDIEIKWIDSEKLNSTNVKTLLNDCDGILVPH
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEC
GFGPRAIEGKILAIQYAREQKIPFFGICFGMQLAVVEYARNVLFLDGANSTEIDEKTPHP
CCCCCCCCCEEEEEEEHHCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
VITKKIKNSNLGGTLRLGSYRCCLQKNSKSEAIYQQTTIYERHRHRFEMNPSYVSLFEKN
HHHHHHCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
NDFLVSGFNPEKNLAEIIELKNHPWFMAVQFHPEFLSRPLKPHPLFKSFVEAALLKNGKN
CCEEEECCCCCHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCCCC
IKKSKLS
CHHCCCC
>Mature Secondary Structure
MNNKKLKSKFIFITGGVVSSLGKGITAASIGNILKNRGFKVSIQKLDPYINIDPGTMSPY
CCCCEEECEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCC
QHGEVFVTNDGAETDLDLGHYERFLDENMSKSSNITAGQIYQSVINKEREGKYLGKTVQV
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
IPHITEEIKKKIIKAAIVHQSDIVIVEIGGTVGDIESAPFLEAIRQFRYEVGYHNVLYLH
HHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEHH
TTLVPYLKKAQEIKTKPTQHSVKELRALGIQPQILVLRSEISINKEIKNKIASLCDINPQ
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHH
AIFEALDVDILYQIILNLFEQRIDHFILNHFQLPNNIQIDLKDWRSLINCIQNLKEKVVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
GLVGKYVILHDAYLSIVESLKHAAYYYNSDIEIKWIDSEKLNSTNVKTLLNDCDGILVPH
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEECCCCCCCCCHHHHHHHCCCCEEEEC
GFGPRAIEGKILAIQYAREQKIPFFGICFGMQLAVVEYARNVLFLDGANSTEIDEKTPHP
CCCCCCCCCEEEEEEEHHCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCC
VITKKIKNSNLGGTLRLGSYRCCLQKNSKSEAIYQQTTIYERHRHRFEMNPSYVSLFEKN
HHHHHHCCCCCCCEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
NDFLVSGFNPEKNLAEIIELKNHPWFMAVQFHPEFLSRPLKPHPLFKSFVEAALLKNGKN
CCEEEECCCCCHHHHHHHHHCCCCEEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHCCCC
IKKSKLS
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA