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The map label for this gene is hisS

Identifier: 197294530

GI number: 197294530

Start: 481187

End: 482434

Strand: Direct

Name: hisS

Synonym: PAa_0485

Alternate gene names: 197294530

Gene position: 481187-482434 (Clockwise)

Preceding gene: 197294529

Following gene: 197294531

Centisome position: 54.68

GC content: 29.81

Gene sequence:

>1248_bases
ATGTTTTATAAGATCAAAGGCACTCATGATTTATTACCAACCCAAACAACTTCTTGGCAAAAAGTGGAAAATTCTTTTCA
TTCTTTTTTTAAAAAACATCAATTTCAAGAAATAAGAACACCCATTATGGAATATGCAGAAGTTTTTCATCGCGCTGCCC
AACATTCCGAAATGGTTTCTAAAGAAACTTATGCTTTTCCAGATAAAAAAAACCGTTTAATTACTTTAAGACCTGAAGGG
ACGGCAGGCATAGTTCGTAGTTATATTGAAAATAAATTAGATCAAACAAATAAGTTGCAAAAATTTTATTATTATGGTCC
TTATTTTCGTTATGAAAGACCTCAATTCGGTCGTTATCGTCAATTTCATCAATTAGGAGTTGAAGTTTTAGGAAAAACAA
CCCCTTTTTTAGACATTGAAGTAATTACATTAATTTATCAGTTTTTAAAATCTTTAGGACTTGATGATATTACTATTCAA
ATCAATTCTTTAGGAAGTAAAGAAGACCATCTGGATTATGTGGATATTTTTAAACAATATTTAAAAATCTATAGCAATTA
TTTGTGTGGTTTGTGTCACGAAAGATTAAATAAAAACCCTTTACGGATTTGGGATTGTAAAACGTGTCATTTACAAGACT
TTTTAAAGCAAGCTCCTAAAATTTTTGATTCCCTTTCTCCTAAATCCAAAAAAAGATTTCAAACGGTTTTGAATGGTTTA
GAAGCAATGAAAGTTAATTTTAAAACTTGTCATGATTTAGTGCGTGGACTTGATTATTATACGCATACAGTTTTTGAAAT
CACCTTTAACAAAATGGTTTTGGGCGGAGGAGGTTGTTACGATCATTTAGTGGCTGCTTTGGGAGGAAATCAACTTTCTG
GGATTGGTTTTGCTTTAGGAATGGAAAGGTTAATGTTAACTTTACAAGAAAATAATAATTTTTTTGTTTCTCCTCAACCT
TCTTTAAAACTCTTTTTATTTATTTTAGACCCAGTTTTTTTTCATCATGGACTTACTTTAGTACACATACTCCGTAATGA
AGGTTTTTGCGTAGAATTTAATTATTCTTTTACGACTTTCACCAAAGGATTAAAAGAGGCTTTGAAATGTCTTCCAAACT
ATCTTTTAATTTTAGGACATCAAGAATTTGCCAAAAAACAAATTACTATCAAAAATATCAAAACCCATACCCAAACAACA
ATCGATCAAAAAGATCTTATTTTTTATTTCCAACAGCAAAGAGTTTAA

Upstream 100 bases:

>100_bases
TGGTATAATAATAAGTAGTTTTGGTTTTTCAATCGATTAAAATTATTAAAATTGAGAAATTTTTTTCTGATTCGCAAATA
TAGATAAAGGGATAAAAGAA

Downstream 100 bases:

>100_bases
AGAGGTTTTTATGAAAAACAAATATAGCCATTACAATAACGAATTAACACTTCAAAACAAAGGAGAATCAGTTTTTTTAA
AAGGTTTTCTTTTTCGCAAG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MFYKIKGTHDLLPTQTTSWQKVENSFHSFFKKHQFQEIRTPIMEYAEVFHRAAQHSEMVSKETYAFPDKKNRLITLRPEG
TAGIVRSYIENKLDQTNKLQKFYYYGPYFRYERPQFGRYRQFHQLGVEVLGKTTPFLDIEVITLIYQFLKSLGLDDITIQ
INSLGSKEDHLDYVDIFKQYLKIYSNYLCGLCHERLNKNPLRIWDCKTCHLQDFLKQAPKIFDSLSPKSKKRFQTVLNGL
EAMKVNFKTCHDLVRGLDYYTHTVFEITFNKMVLGGGGCYDHLVAALGGNQLSGIGFALGMERLMLTLQENNNFFVSPQP
SLKLFLFILDPVFFHHGLTLVHILRNEGFCVEFNYSFTTFTKGLKEALKCLPNYLLILGHQEFAKKQITIKNIKTHTQTT
IDQKDLIFYFQQQRV

Sequences:

>Translated_415_residues
MFYKIKGTHDLLPTQTTSWQKVENSFHSFFKKHQFQEIRTPIMEYAEVFHRAAQHSEMVSKETYAFPDKKNRLITLRPEG
TAGIVRSYIENKLDQTNKLQKFYYYGPYFRYERPQFGRYRQFHQLGVEVLGKTTPFLDIEVITLIYQFLKSLGLDDITIQ
INSLGSKEDHLDYVDIFKQYLKIYSNYLCGLCHERLNKNPLRIWDCKTCHLQDFLKQAPKIFDSLSPKSKKRFQTVLNGL
EAMKVNFKTCHDLVRGLDYYTHTVFEITFNKMVLGGGGCYDHLVAALGGNQLSGIGFALGMERLMLTLQENNNFFVSPQP
SLKLFLFILDPVFFHHGLTLVHILRNEGFCVEFNYSFTTFTKGLKEALKCLPNYLLILGHQEFAKKQITIKNIKTHTQTT
IDQKDLIFYFQQQRV
>Mature_415_residues
MFYKIKGTHDLLPTQTTSWQKVENSFHSFFKKHQFQEIRTPIMEYAEVFHRAAQHSEMVSKETYAFPDKKNRLITLRPEG
TAGIVRSYIENKLDQTNKLQKFYYYGPYFRYERPQFGRYRQFHQLGVEVLGKTTPFLDIEVITLIYQFLKSLGLDDITIQ
INSLGSKEDHLDYVDIFKQYLKIYSNYLCGLCHERLNKNPLRIWDCKTCHLQDFLKQAPKIFDSLSPKSKKRFQTVLNGL
EAMKVNFKTCHDLVRGLDYYTHTVFEITFNKMVLGGGGCYDHLVAALGGNQLSGIGFALGMERLMLTLQENNNFFVSPQP
SLKLFLFILDPVFFHHGLTLVHILRNEGFCVEFNYSFTTFTKGLKEALKCLPNYLLILGHQEFAKKQITIKNIKTHTQTT
IDQKDLIFYFQQQRV

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI6996014, Length=457, Percent_Identity=23.6323851203501, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1788861, Length=415, Percent_Identity=36.3855421686747, Blast_Score=275, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=22.4789915966387, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PHYAS (B1VA46)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799071.1
- ProteinModelPortal:   B1VA46
- SMR:   B1VA46
- GeneID:   6798876
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 48586; Mature: 48586

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYKIKGTHDLLPTQTTSWQKVENSFHSFFKKHQFQEIRTPIMEYAEVFHRAAQHSEMVS
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETYAFPDKKNRLITLRPEGTAGIVRSYIENKLDQTNKLQKFYYYGPYFRYERPQFGRYR
HHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCHHHHH
QFHQLGVEVLGKTTPFLDIEVITLIYQFLKSLGLDDITIQINSLGSKEDHLDYVDIFKQY
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKIYSNYLCGLCHERLNKNPLRIWDCKTCHLQDFLKQAPKIFDSLSPKSKKRFQTVLNGL
HHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EAMKVNFKTCHDLVRGLDYYTHTVFEITFNKMVLGGGGCYDHLVAALGGNQLSGIGFALG
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECEEEECCCCHHHHHHHHHCCCCCCHHHHHHH
MERLMLTLQENNNFFVSPQPSLKLFLFILDPVFFHHGLTLVHILRNEGFCVEFNYSFTTF
HHHHHEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCHHHH
TKGLKEALKCLPNYLLILGHQEFAKKQITIKNIKTHTQTTIDQKDLIFYFQQQRV
HHHHHHHHHHCCHHEEEECCHHHHHHCEEHHHCCHHHHHCCCCHHHHHHEECCCC
>Mature Secondary Structure
MFYKIKGTHDLLPTQTTSWQKVENSFHSFFKKHQFQEIRTPIMEYAEVFHRAAQHSEMVS
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETYAFPDKKNRLITLRPEGTAGIVRSYIENKLDQTNKLQKFYYYGPYFRYERPQFGRYR
HHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEECCEEECCCCCHHHHH
QFHQLGVEVLGKTTPFLDIEVITLIYQFLKSLGLDDITIQINSLGSKEDHLDYVDIFKQY
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHH
LKIYSNYLCGLCHERLNKNPLRIWDCKTCHLQDFLKQAPKIFDSLSPKSKKRFQTVLNGL
HHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EAMKVNFKTCHDLVRGLDYYTHTVFEITFNKMVLGGGGCYDHLVAALGGNQLSGIGFALG
HHHHCCHHHHHHHHHHHHHHHHHHEEEEECEEEECCCCHHHHHHHHHCCCCCCHHHHHHH
MERLMLTLQENNNFFVSPQPSLKLFLFILDPVFFHHGLTLVHILRNEGFCVEFNYSFTTF
HHHHHEEEECCCCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEECCHHHH
TKGLKEALKCLPNYLLILGHQEFAKKQITIKNIKTHTQTTIDQKDLIFYFQQQRV
HHHHHHHHHHCCHHEEEECCHHHHHHCEEHHHCCHHHHHCCCCHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA