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The map label for this gene is aspS [H]

Identifier: 197294531

GI number: 197294531

Start: 482445

End: 484130

Strand: Direct

Name: aspS [H]

Synonym: PAa_0486

Alternate gene names: 197294531

Gene position: 482445-484130 (Clockwise)

Preceding gene: 197294530

Following gene: 197294532

Centisome position: 54.83

GC content: 30.6

Gene sequence:

>1686_bases
ATGAAAAACAAATATAGCCATTACAATAACGAATTAACACTTCAAAACAAAGGAGAATCAGTTTTTTTAAAAGGTTTTCT
TTTTCGCAAGAGAAATTTAGGCAAAAATATTTTTTTAGATTTGCGTGATGTTTCAGGAGTCATTCAATTATTAGTTAAAG
AAAACAACCCTCAATATCAGCAAATTACTACTTTAAAACCAGAAACAGTGATGGAAGTTAAAGGTGTTGTTGTTGAAAGA
ATTAATAAAAACAGCAAATTAAAAACAGGAGAAATTGAAATTTTAGTTTCTTTTGTAAATGTTTTGAGCGAACCCCAAAC
GCTTCCTTTAAACGTTTTTCAAAGTCAAGACAGTCTTGAAGAAACGAGGCTTAAATATCGTTATTTAGATTTGCGCAATC
CTGACGTCAAACATTTTTTAATTCAACGACACCATATTACTCAAAGTATTCGTCAAACTCTTTTAAAAAATCATTTTTTA
GAACTTGAAACCCCTATTTTATCTAAATCAACCCCAGAAGGAGCGAGGGATTATTTGGTTCCTTCAAGAATTTATCCAGG
TAATTTCTATGCTTTACCTCAATCGCCTCAAATTTATAAGCAATTATATATGATTGCTGGTTTTGAAAAATATTTTCAAG
TAGCTCGTTGTTTCCGTGATGAGGATTTAAGAGCTGATCGCCAACCTGAATTTACTCAAATAGATATTGAAACCTCTTTT
TGCACCCAAGCAGAAATTATGACCTTAACTGAAGAAATTATTATTGATTTATTTAAAAATATTTGGCAACAAAACCTTTC
CAAACCGTTTGCAACTTTAACTTACAACCAAGCTTTAGAATTGTATGGAACTGACAAACCCGATTTAAGAAACCCTTTAA
AAATTTTTGATTTTACTCCTTTTTTTACTTCTGATTTTGGAAAAGATAACCTTAAAGGCTTTAAAGTTTCCAAGACTCCT
TTTTTAACGCGACGTCAAGTAGAAAAATACCAATTGTTTTTTGAAAATAATTTTTCTTTAAAACTTTTAACTTTTGAGAA
AAAAAATGATAAAATTACTGGTGGAATATCAAAATTTATTAAAGATAATTCTTTTTTGCAAAATGATGAAATTTGCTTTG
TGGTTTCAGGCGTAAAAGAAAAAGTCCATAAATATTTGGGTATCTTCAGAAATAAATTAGCCGCTGATTTATCTTTAATC
AATGAAAATCAAGAATCTTTGTTATGGATAGTTGATTTCCCTTTGTTGGAATTTGATGCTTCTAATAATAGGTTTTACTC
TTTGCATCATCCCTTTACGGCCCCTTGTAATCTTGATTCTTTAAAAAAAGACCCTCAAAAAGCTTTGGCTTTGGCATATG
ATTTAGTGTGGAATGGTTATGAAGTTGGTGGCGGTTCTTTTAGGATTAACAACCCCGAAACCCAAGGAATTATTTTTAAG
CTTTTAGGTTTTAGTCAACAAGAAATTCAAAAAAAATTTGGTTTTTTAATTGAAGCCCTTCAATACGGCACCCCTCCTCA
TGGTGGTTTGGCTTTGGGTCTTGATCGTTTAGTGATGCTTTTTACCAAAACTCAAAATATCAAAGATGTTATTGCTTTTC
CTAAAACGCAAAGTGCTAAAGATTTGATGCTAGATTCTCCAGGAGAAGTTGATCAAGAACAATTAAAAATTTTAAAACTT
AAATGA

Upstream 100 bases:

>100_bases
CAAATTACTATCAAAAATATCAAAACCCATACCCAAACAACAATCGATCAAAAAGATCTTATTTTTTATTTCCAACAGCA
AAGAGTTTAAAGAGGTTTTT

Downstream 100 bases:

>100_bases
GAAAAAACATGGTTCAATGAAACTAGCTTTAAAATTAGATTTTCAAAAAACTTATATTATAGCTGTTAGCGGCGGCGTTG
ATTCGATGGTTTTATTAGAT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MKNKYSHYNNELTLQNKGESVFLKGFLFRKRNLGKNIFLDLRDVSGVIQLLVKENNPQYQQITTLKPETVMEVKGVVVER
INKNSKLKTGEIEILVSFVNVLSEPQTLPLNVFQSQDSLEETRLKYRYLDLRNPDVKHFLIQRHHITQSIRQTLLKNHFL
ELETPILSKSTPEGARDYLVPSRIYPGNFYALPQSPQIYKQLYMIAGFEKYFQVARCFRDEDLRADRQPEFTQIDIETSF
CTQAEIMTLTEEIIIDLFKNIWQQNLSKPFATLTYNQALELYGTDKPDLRNPLKIFDFTPFFTSDFGKDNLKGFKVSKTP
FLTRRQVEKYQLFFENNFSLKLLTFEKKNDKITGGISKFIKDNSFLQNDEICFVVSGVKEKVHKYLGIFRNKLAADLSLI
NENQESLLWIVDFPLLEFDASNNRFYSLHHPFTAPCNLDSLKKDPQKALALAYDLVWNGYEVGGGSFRINNPETQGIIFK
LLGFSQQEIQKKFGFLIEALQYGTPPHGGLALGLDRLVMLFTKTQNIKDVIAFPKTQSAKDLMLDSPGEVDQEQLKILKL
K

Sequences:

>Translated_561_residues
MKNKYSHYNNELTLQNKGESVFLKGFLFRKRNLGKNIFLDLRDVSGVIQLLVKENNPQYQQITTLKPETVMEVKGVVVER
INKNSKLKTGEIEILVSFVNVLSEPQTLPLNVFQSQDSLEETRLKYRYLDLRNPDVKHFLIQRHHITQSIRQTLLKNHFL
ELETPILSKSTPEGARDYLVPSRIYPGNFYALPQSPQIYKQLYMIAGFEKYFQVARCFRDEDLRADRQPEFTQIDIETSF
CTQAEIMTLTEEIIIDLFKNIWQQNLSKPFATLTYNQALELYGTDKPDLRNPLKIFDFTPFFTSDFGKDNLKGFKVSKTP
FLTRRQVEKYQLFFENNFSLKLLTFEKKNDKITGGISKFIKDNSFLQNDEICFVVSGVKEKVHKYLGIFRNKLAADLSLI
NENQESLLWIVDFPLLEFDASNNRFYSLHHPFTAPCNLDSLKKDPQKALALAYDLVWNGYEVGGGSFRINNPETQGIIFK
LLGFSQQEIQKKFGFLIEALQYGTPPHGGLALGLDRLVMLFTKTQNIKDVIAFPKTQSAKDLMLDSPGEVDQEQLKILKL
K
>Mature_561_residues
MKNKYSHYNNELTLQNKGESVFLKGFLFRKRNLGKNIFLDLRDVSGVIQLLVKENNPQYQQITTLKPETVMEVKGVVVER
INKNSKLKTGEIEILVSFVNVLSEPQTLPLNVFQSQDSLEETRLKYRYLDLRNPDVKHFLIQRHHITQSIRQTLLKNHFL
ELETPILSKSTPEGARDYLVPSRIYPGNFYALPQSPQIYKQLYMIAGFEKYFQVARCFRDEDLRADRQPEFTQIDIETSF
CTQAEIMTLTEEIIIDLFKNIWQQNLSKPFATLTYNQALELYGTDKPDLRNPLKIFDFTPFFTSDFGKDNLKGFKVSKTP
FLTRRQVEKYQLFFENNFSLKLLTFEKKNDKITGGISKFIKDNSFLQNDEICFVVSGVKEKVHKYLGIFRNKLAADLSLI
NENQESLLWIVDFPLLEFDASNNRFYSLHHPFTAPCNLDSLKKDPQKALALAYDLVWNGYEVGGGSFRINNPETQGIIFK
LLGFSQQEIQKKFGFLIEALQYGTPPHGGLALGLDRLVMLFTKTQNIKDVIAFPKTQSAKDLMLDSPGEVDQEQLKILKL
K

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=584, Percent_Identity=39.554794520548, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI45439306, Length=277, Percent_Identity=29.2418772563177, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI194272210, Length=553, Percent_Identity=24.2314647377938, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI5031815, Length=273, Percent_Identity=27.4725274725275, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1788173, Length=571, Percent_Identity=42.2066549912434, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1789256, Length=288, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790571, Length=295, Percent_Identity=28.135593220339, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32566633, Length=580, Percent_Identity=37.5862068965517, Blast_Score=330, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17551876, Length=289, Percent_Identity=26.643598615917, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=302, Percent_Identity=22.5165562913907, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=251, Percent_Identity=25.8964143426295, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=251, Percent_Identity=25.8964143426295, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17535927, Length=251, Percent_Identity=25.8964143426295, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=639, Percent_Identity=34.1158059467919, Blast_Score=273, Evalue=6e-74,
Organism=Saccharomyces cerevisiae, GI6320242, Length=267, Percent_Identity=25.8426966292135, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323011, Length=270, Percent_Identity=26.2962962962963, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=262, Percent_Identity=23.6641221374046, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24584738, Length=598, Percent_Identity=33.9464882943144, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24640849, Length=304, Percent_Identity=25.9868421052632, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24640851, Length=304, Percent_Identity=25.9868421052632, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=27.4809160305344, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 64934; Mature: 64934

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKYSHYNNELTLQNKGESVFLKGFLFRKRNLGKNIFLDLRDVSGVIQLLVKENNPQYQ
CCCCCCCCCCEEEEECCCCEEEEHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCEE
QITTLKPETVMEVKGVVVERINKNSKLKTGEIEILVSFVNVLSEPQTLPLNVFQSQDSLE
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
ETRLKYRYLDLRNPDVKHFLIQRHHITQSIRQTLLKNHFLELETPILSKSTPEGARDYLV
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHCCC
PSRIYPGNFYALPQSPQIYKQLYMIAGFEKYFQVARCFRDEDLRADRQPEFTQIDIETSF
CCEECCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
CTQAEIMTLTEEIIIDLFKNIWQQNLSKPFATLTYNQALELYGTDKPDLRNPLKIFDFTP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCEECCCCCCCCCCCCEEEECCC
FFTSDFGKDNLKGFKVSKTPFLTRRQVEKYQLFFENNFSLKLLTFEKKNDKITGGISKFI
CHHCCCCCCCCCCEEECCCCCHHHHHHHHEEEEEECCCEEEEEEEECCCCCCHHHHHHHH
KDNSFLQNDEICFVVSGVKEKVHKYLGIFRNKLAADLSLINENQESLLWIVDFPLLEFDA
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEC
SNNRFYSLHHPFTAPCNLDSLKKDPQKALALAYDLVWNGYEVGGGSFRINNPETQGIIFK
CCCEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCHHHH
LLGFSQQEIQKKFGFLIEALQYGTPPHGGLALGLDRLVMLFTKTQNIKDVIAFPKTQSAK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
DLMLDSPGEVDQEQLKILKLK
HHCCCCCCCCCHHHHEEEECC
>Mature Secondary Structure
MKNKYSHYNNELTLQNKGESVFLKGFLFRKRNLGKNIFLDLRDVSGVIQLLVKENNPQYQ
CCCCCCCCCCEEEEECCCCEEEEHHHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCEE
QITTLKPETVMEVKGVVVERINKNSKLKTGEIEILVSFVNVLSEPQTLPLNVFQSQDSLE
EEEECCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
ETRLKYRYLDLRNPDVKHFLIQRHHITQSIRQTLLKNHFLELETPILSKSTPEGARDYLV
HHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCHHCCC
PSRIYPGNFYALPQSPQIYKQLYMIAGFEKYFQVARCFRDEDLRADRQPEFTQIDIETSF
CCEECCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCC
CTQAEIMTLTEEIIIDLFKNIWQQNLSKPFATLTYNQALELYGTDKPDLRNPLKIFDFTP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCEECCCCCCCCCCCCEEEECCC
FFTSDFGKDNLKGFKVSKTPFLTRRQVEKYQLFFENNFSLKLLTFEKKNDKITGGISKFI
CHHCCCCCCCCCCEEECCCCCHHHHHHHHEEEEEECCCEEEEEEEECCCCCCHHHHHHHH
KDNSFLQNDEICFVVSGVKEKVHKYLGIFRNKLAADLSLINENQESLLWIVDFPLLEFDA
CCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEC
SNNRFYSLHHPFTAPCNLDSLKKDPQKALALAYDLVWNGYEVGGGSFRINNPETQGIIFK
CCCEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCHHHH
LLGFSQQEIQKKFGFLIEALQYGTPPHGGLALGLDRLVMLFTKTQNIKDVIAFPKTQSAK
HHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCC
DLMLDSPGEVDQEQLKILKLK
HHCCCCCCCCCHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA