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The map label for this gene is rfbD

Identifier: 170756261

GI number: 170756261

Start: 2879062

End: 2879958

Strand: Reverse

Name: rfbD

Synonym: CLD_1862

Alternate gene names: 170756261

Gene position: 2879958-2879062 (Counterclockwise)

Preceding gene: 170757122

Following gene: 170757117

Centisome position: 72.76

GC content: 28.43

Gene sequence:

>897_bases
ATGGTGAAAATACTTATAACCGGAGCTAAAGGACAGCTAGGAAGTCAAATTGTAAATATTTTAAAAGCTAGTAAATCTGA
ATTAGGAGTAATTGATAATATATATATAAATACTGAAATAATTGCAACAGACTCTAAAGAACTAGATATAACTAACTTAA
ATAGTGCTAAGAGATTTATGGAGAAATGTTTGCCAAATATTGTAATTAATTGTGCGGCATATACTAATGTAGATAGTTGT
GAAGATAATTATGAATTAGCCTTTAAAGTTAATTCATTAGGTGCAAGAAATTTAGCTATAGCCTGTGAAAAAATAAAAGC
TAAGCTAATTCATATATCTACAGACTATGTTTTTGAAGGAAATGGACAAGCTCCCTATAAAGAGTATGATTTAAATAATC
CAGTAAGTGTATACGGGAAATCAAAATTATTAGGTGAACGATATGTTAAAGAGTTTTGTAGTAGATATTTTATAGTTAGG
ACATCATGGCTTTACGGATATAGTGGGAATAATTTTGTAAAAACTATAATAAAAGCTGCAAAGGGAAAAAGACAATTACA
AGTTGTAGATGATCAGATAGGAAATCCGACTAATGCAGAAGATTTAGCATACCATATCTTAAAATTGCCTTTAACTGAAG
AATATGGCATATACCATTGTACAGGAAGTGGGGAATGCAGTTGGTATGATTTCGCTTGTAAGATAGTTGAGTTTTATAAT
ATAGATTGTAAAGTAGATAGAATGAAGTCAGATAAATTAAATAGAAAAGCTAAAAGACCATATTTTTCATCGTTAGATAA
TATGATGTTAAGATGTACTATAGGAGATGAAATGAGAAATTGGCAAGACGCATTAAAAGTATTTATAAATACATTAAGAG
AAAACAATTTATTATAA

Upstream 100 bases:

>100_bases
AGAACAGATTATGGGCAATATCTTATAAAATTAATAAATGAAGATTTTATGATAGATTTTAATACTTTAACAGAAGTAGC
AATAACTACAAGTAGGTGAT

Downstream 100 bases:

>100_bases
AGAAAGAGGGTGACTAATTTGAAAACTTATTTAGTTACTGGAGGGGCGGGATTTATTGGGTCAAATTTTATACTTTATAT
GCTAGATAAGTATAAAAATA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MVKILITGAKGQLGSQIVNILKASKSELGVIDNIYINTEIIATDSKELDITNLNSAKRFMEKCLPNIVINCAAYTNVDSC
EDNYELAFKVNSLGARNLAIACEKIKAKLIHISTDYVFEGNGQAPYKEYDLNNPVSVYGKSKLLGERYVKEFCSRYFIVR
TSWLYGYSGNNFVKTIIKAAKGKRQLQVVDDQIGNPTNAEDLAYHILKLPLTEEYGIYHCTGSGECSWYDFACKIVEFYN
IDCKVDRMKSDKLNRKAKRPYFSSLDNMMLRCTIGDEMRNWQDALKVFINTLRENNLL

Sequences:

>Translated_298_residues
MVKILITGAKGQLGSQIVNILKASKSELGVIDNIYINTEIIATDSKELDITNLNSAKRFMEKCLPNIVINCAAYTNVDSC
EDNYELAFKVNSLGARNLAIACEKIKAKLIHISTDYVFEGNGQAPYKEYDLNNPVSVYGKSKLLGERYVKEFCSRYFIVR
TSWLYGYSGNNFVKTIIKAAKGKRQLQVVDDQIGNPTNAEDLAYHILKLPLTEEYGIYHCTGSGECSWYDFACKIVEFYN
IDCKVDRMKSDKLNRKAKRPYFSSLDNMMLRCTIGDEMRNWQDALKVFINTLRENNLL
>Mature_298_residues
MVKILITGAKGQLGSQIVNILKASKSELGVIDNIYINTEIIATDSKELDITNLNSAKRFMEKCLPNIVINCAAYTNVDSC
EDNYELAFKVNSLGARNLAIACEKIKAKLIHISTDYVFEGNGQAPYKEYDLNNPVSVYGKSKLLGERYVKEFCSRYFIVR
TSWLYGYSGNNFVKTIIKAAKGKRQLQVVDDQIGNPTNAEDLAYHILKLPLTEEYGIYHCTGSGECSWYDFACKIVEFYN
IDCKVDRMKSDKLNRKAKRPYFSSLDNMMLRCTIGDEMRNWQDALKVFINTLRENNLL

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=273, Percent_Identity=25.6410256410256, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI11034825, Length=273, Percent_Identity=25.6410256410256, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788352, Length=302, Percent_Identity=33.112582781457, Blast_Score=177, Evalue=5e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 33885; Mature: 33885

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKILITGAKGQLGSQIVNILKASKSELGVIDNIYINTEIIATDSKELDITNLNSAKRFM
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCEEECCHHHHHHH
EKCLPNIVINCAAYTNVDSCEDNYELAFKVNSLGARNLAIACEKIKAKLIHISTDYVFEG
HHHCHHHEEEEHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEEEEEEEECEEEEC
NGQAPYKEYDLNNPVSVYGKSKLLGERYVKEFCSRYFIVRTSWLYGYSGNNFVKTIIKAA
CCCCCCHHCCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHHHHHHHHH
KGKRQLQVVDDQIGNPTNAEDLAYHILKLPLTEEYGIYHCTGSGECSWYDFACKIVEFYN
CCCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHEEEEHHCC
IDCKVDRMKSDKLNRKAKRPYFSSLDNMMLRCTIGDEMRNWQDALKVFINTLRENNLL
CCEEEECCCHHHHHHHHCCCHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKILITGAKGQLGSQIVNILKASKSELGVIDNIYINTEIIATDSKELDITNLNSAKRFM
CEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEECCCCCEEECCHHHHHHH
EKCLPNIVINCAAYTNVDSCEDNYELAFKVNSLGARNLAIACEKIKAKLIHISTDYVFEG
HHHCHHHEEEEHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHEEEEEEEEECEEEEC
NGQAPYKEYDLNNPVSVYGKSKLLGERYVKEFCSRYFIVRTSWLYGYSGNNFVKTIIKAA
CCCCCCHHCCCCCCEEECCCHHHHHHHHHHHHHHHEEEEEEEEEEECCCCHHHHHHHHHH
KGKRQLQVVDDQIGNPTNAEDLAYHILKLPLTEEYGIYHCTGSGECSWYDFACKIVEFYN
CCCCEEEEEHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHEEEEHHCC
IDCKVDRMKSDKLNRKAKRPYFSSLDNMMLRCTIGDEMRNWQDALKVFINTLRENNLL
CCEEEECCCHHHHHHHHCCCHHHHHCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]