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The map label for this gene is rfbA

Identifier: 170757122

GI number: 170757122

Start: 2879960

End: 2880880

Strand: Reverse

Name: rfbA

Synonym: CLD_1861

Alternate gene names: 170757122

Gene position: 2880880-2879960 (Counterclockwise)

Preceding gene: 170756450

Following gene: 170756261

Centisome position: 72.78

GC content: 29.97

Gene sequence:

>921_bases
ATGAAAGGAATAATATTAGCAGGTGGAGCTGGGACAAGATTATATCCAATAACTAAGTCCATTTCAAAACAAATATTACC
TATATATGATAAACCAATGATATATTATCCAATGTCTGTACTTATGCTAGCTGGTATAAGAGAAATACTTATTATTTCTA
CTCCTAGAGACATACATGATTTTAAAGAATTATTTAATGATGGAAGTAAATTGGGATTAAATATACAATATGCAATCCAA
GAAGAACCAAGAGGACTTGCAGAAGCTTTCATAATAGGGGAAAGGTTTATAGGAAACGATAATGTATGCTTAATACTTGG
TGATAATATATTCTTTGGATACGGATTTACAGAAAGATTAGAAAGAGCAGCTTATAGAAGCGAAGGAGCAACTATATTTG
GATACCATGTAAGTAATCCAAAAGATTTTGGTGTAGTAGAATTTGATAAAAATAATGATGTAATATCTATAGAAGAAAAA
CCGGAGATTCCAAAGTCAAATTATGCAGTTCCAGGACTTTATTTTTATGATAATGATGTAATAGATATTGCTAAAATTAT
AAAGCCATCTAATAGAGGAGAATTAGAAATAACCTCTGTAAATAATGAATATTTAAAAAAAGGGAAGTTAAAGGTAGAGC
TTTTTGGAAGAGGGATGGCTTGGTTTGATACAGGGACTCATAGAGGATTATTAGATGCAGCTAACTTTGTTGAGGCAATA
CAAACAAGACAAGGGCTATATGTAGCTTGTCTTGAAGAAATAGCATTTAAAAAGGGATATATAGATGAAGAAGAATTATT
GAAATTAGCACAGCCATTATCTAGAACAGATTATGGGCAATATCTTATAAAATTAATAAATGAAGATTTTATGATAGATT
TTAATACTTTAACAGAAGTAGCAATAACTACAAGTAGGTGA

Upstream 100 bases:

>100_bases
GAAACAAGTTATCTATATAAAAATATTGAAAATAAAAGGGTTAAAGCAAAAGTTACGAAACAACTTTTGACAATACCCAT
TGTAAAGGGGTGTGAATAAT

Downstream 100 bases:

>100_bases
TATGGTGAAAATACTTATAACCGGAGCTAAAGGACAGCTAGGAAGTCAAATTGTAAATATTTTAAAAGCTAGTAAATCTG
AATTAGGAGTAATTGATAAT

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: dTDP-glucose pyrophosphorylase; dTDP-glucose synthase [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKGIILAGGAGTRLYPITKSISKQILPIYDKPMIYYPMSVLMLAGIREILIISTPRDIHDFKELFNDGSKLGLNIQYAIQ
EEPRGLAEAFIIGERFIGNDNVCLILGDNIFFGYGFTERLERAAYRSEGATIFGYHVSNPKDFGVVEFDKNNDVISIEEK
PEIPKSNYAVPGLYFYDNDVIDIAKIIKPSNRGELEITSVNNEYLKKGKLKVELFGRGMAWFDTGTHRGLLDAANFVEAI
QTRQGLYVACLEEIAFKKGYIDEEELLKLAQPLSRTDYGQYLIKLINEDFMIDFNTLTEVAITTSR

Sequences:

>Translated_306_residues
MKGIILAGGAGTRLYPITKSISKQILPIYDKPMIYYPMSVLMLAGIREILIISTPRDIHDFKELFNDGSKLGLNIQYAIQ
EEPRGLAEAFIIGERFIGNDNVCLILGDNIFFGYGFTERLERAAYRSEGATIFGYHVSNPKDFGVVEFDKNNDVISIEEK
PEIPKSNYAVPGLYFYDNDVIDIAKIIKPSNRGELEITSVNNEYLKKGKLKVELFGRGMAWFDTGTHRGLLDAANFVEAI
QTRQGLYVACLEEIAFKKGYIDEEELLKLAQPLSRTDYGQYLIKLINEDFMIDFNTLTEVAITTSR
>Mature_306_residues
MKGIILAGGAGTRLYPITKSISKQILPIYDKPMIYYPMSVLMLAGIREILIISTPRDIHDFKELFNDGSKLGLNIQYAIQ
EEPRGLAEAFIIGERFIGNDNVCLILGDNIFFGYGFTERLERAAYRSEGATIFGYHVSNPKDFGVVEFDKNNDVISIEEK
PEIPKSNYAVPGLYFYDNDVIDIAKIIKPSNRGELEITSVNNEYLKKGKLKVELFGRGMAWFDTGTHRGLLDAANFVEAI
QTRQGLYVACLEEIAFKKGYIDEEELLKLAQPLSRTDYGQYLIKLINEDFMIDFNTLTEVAITTSR

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=252, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI11761619, Length=252, Percent_Identity=25, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788351, Length=287, Percent_Identity=62.3693379790941, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790224, Length=287, Percent_Identity=61.3240418118467, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787488, Length=275, Percent_Identity=24.7272727272727, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788355, Length=291, Percent_Identity=21.9931271477663, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=239, Percent_Identity=25.1046025104602, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320148, Length=250, Percent_Identity=26.4, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355443, Length=247, Percent_Identity=24.6963562753036, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24644084, Length=247, Percent_Identity=24.6963562753036, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 34572; Mature: 34572

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIILAGGAGTRLYPITKSISKQILPIYDKPMIYYPMSVLMLAGIREILIISTPRDIHD
CCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCHHHH
FKELFNDGSKLGLNIQYAIQEEPRGLAEAFIIGERFIGNDNVCLILGDNIFFGYGFTERL
HHHHHCCCCEEEEEEEEEECCCCCCHHHHHEEHHEEECCCCEEEEEECCEEEECCHHHHH
ERAAYRSEGATIFGYHVSNPKDFGVVEFDKNNDVISIEEKPEIPKSNYAVPGLYFYDNDV
HHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCH
IDIAKIIKPSNRGELEITSVNNEYLKKGKLKVELFGRGMAWFDTGTHRGLLDAANFVEAI
HHHHHHHCCCCCCCEEEEECCHHHHHCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHH
QTRQGLYVACLEEIAFKKGYIDEEELLKLAQPLSRTDYGQYLIKLINEDFMIDFNTLTEV
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEE
AITTSR
EEEECC
>Mature Secondary Structure
MKGIILAGGAGTRLYPITKSISKQILPIYDKPMIYYPMSVLMLAGIREILIISTPRDIHD
CCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCEEEEEECCCHHHH
FKELFNDGSKLGLNIQYAIQEEPRGLAEAFIIGERFIGNDNVCLILGDNIFFGYGFTERL
HHHHHCCCCEEEEEEEEEECCCCCCHHHHHEEHHEEECCCCEEEEEECCEEEECCHHHHH
ERAAYRSEGATIFGYHVSNPKDFGVVEFDKNNDVISIEEKPEIPKSNYAVPGLYFYDNDV
HHHHHHCCCCEEEEEECCCCCCCCEEEECCCCCEEEECCCCCCCCCCCCCCEEEEECCCH
IDIAKIIKPSNRGELEITSVNNEYLKKGKLKVELFGRGMAWFDTGTHRGLLDAANFVEAI
HHHHHHHCCCCCCCEEEEECCHHHHHCCCEEEEEEECCEEEEECCCCCCHHHHHHHHHHH
QTRQGLYVACLEEIAFKKGYIDEEELLKLAQPLSRTDYGQYLIKLINEDFMIDFNTLTEV
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEECCCEEE
AITTSR
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]