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The map label for this gene is rfbB

Identifier: 170757117

GI number: 170757117

Start: 2877988

End: 2879043

Strand: Reverse

Name: rfbB

Synonym: CLD_1863

Alternate gene names: 170757117

Gene position: 2879043-2877988 (Counterclockwise)

Preceding gene: 170756261

Following gene: 170755117

Centisome position: 72.74

GC content: 28.79

Gene sequence:

>1056_bases
TTGAAAACTTATTTAGTTACTGGAGGGGCGGGATTTATTGGGTCAAATTTTATACTTTATATGCTAGATAAGTATAAAAA
TATTAAAATAATAAATCTAGATAAATTAACTTATGCAGGGAACTTAGAAAATTTAAAAGAAGTAGAAAGCAATATTAACT
ACTCATTCGTAAATGGTGATATATGTGATAGAGAACTAGTAGAAAGAGTATTTAAAGAAAATAATATAGATTATGTTGTC
AATTTTGCGGCAGAATCACATGTTGACAGAAGTATAAAAGATCCCGAAATTTTTGCAAAAACTAATGTTTTAGGTACAGT
AAATCTTTTAAATGTTGCTAAGATTTCTTGGCAAACTGAAGAAGCATGGGTAGAGAATAAAAAATTCCTTCAAGTTTCTA
CGGATGAGGTTTATGGTTCTTTAGGAGACACAGGATACTTTATGGAAGATACACCACTAGATCCTCATAGTCCATATTCA
TCAAGTAAAGCCAGTGGAGATTTAATGGTAAAGGCATACTTTGATACATATAAAATGCCTGTAAATATTACTAGATGTTC
TAATAATTATGGGCCATATCAATTCCCAGAAAAGTTAATACCACTGATAATAAATAATTGCTTAAATAATAACCCAATTC
CTATATATGGTGACGGTTTAAATGTAAGAGATTGGCTATATGTAGAAGATCATTGCAAAGCAATAGATATGGTAATAAAT
AAGGGCAGTTTAGGTGAAGTGTACAATATAGGTGGGCACAATGAAAAAACAAATATATTTATTGTAAAAGCAATAATAGA
ATACATAAATGAAAATGTTGATAAAACAGTTACAGAAAAGCTGATTAAATATGTAGAAGACAGGAAAGGTCATGATAGAA
GATATGGTATAGATCCAACTAAAATTAAAGAAGATTTAGGTTGGGAACCTAAAACCAAATTTGAAGATGGTATAATTAAG
ACAATAGAATGGTATTTAAGTAATAAAGATTGGATGGAAAACATTATAACAGATAACTATCAGAGTTATTATAAAAAAAT
GTATGGAGAGAATTAG

Upstream 100 bases:

>100_bases
TACTATAGGAGATGAAATGAGAAATTGGCAAGACGCATTAAAAGTATTTATAAATACATTAAGAGAAAACAATTTATTAT
AAAGAAAGAGGGTGACTAAT

Downstream 100 bases:

>100_bases
GAGGAGATTTTATGGGACAATTTAAGTTTACGAAAACTCCTATAGAAGACTTATATGTAATTGAACCAAAAGTTTTTAGT
GATCCTAGAGGATATTTTAT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKTYLVTGGAGFIGSNFILYMLDKYKNIKIINLDKLTYAGNLENLKEVESNINYSFVNGDICDRELVERVFKENNIDYVV
NFAAESHVDRSIKDPEIFAKTNVLGTVNLLNVAKISWQTEEAWVENKKFLQVSTDEVYGSLGDTGYFMEDTPLDPHSPYS
SSKASGDLMVKAYFDTYKMPVNITRCSNNYGPYQFPEKLIPLIINNCLNNNPIPIYGDGLNVRDWLYVEDHCKAIDMVIN
KGSLGEVYNIGGHNEKTNIFIVKAIIEYINENVDKTVTEKLIKYVEDRKGHDRRYGIDPTKIKEDLGWEPKTKFEDGIIK
TIEWYLSNKDWMENIITDNYQSYYKKMYGEN

Sequences:

>Translated_351_residues
MKTYLVTGGAGFIGSNFILYMLDKYKNIKIINLDKLTYAGNLENLKEVESNINYSFVNGDICDRELVERVFKENNIDYVV
NFAAESHVDRSIKDPEIFAKTNVLGTVNLLNVAKISWQTEEAWVENKKFLQVSTDEVYGSLGDTGYFMEDTPLDPHSPYS
SSKASGDLMVKAYFDTYKMPVNITRCSNNYGPYQFPEKLIPLIINNCLNNNPIPIYGDGLNVRDWLYVEDHCKAIDMVIN
KGSLGEVYNIGGHNEKTNIFIVKAIIEYINENVDKTVTEKLIKYVEDRKGHDRRYGIDPTKIKEDLGWEPKTKFEDGIIK
TIEWYLSNKDWMENIITDNYQSYYKKMYGEN
>Mature_351_residues
MKTYLVTGGAGFIGSNFILYMLDKYKNIKIINLDKLTYAGNLENLKEVESNINYSFVNGDICDRELVERVFKENNIDYVV
NFAAESHVDRSIKDPEIFAKTNVLGTVNLLNVAKISWQTEEAWVENKKFLQVSTDEVYGSLGDTGYFMEDTPLDPHSPYS
SSKASGDLMVKAYFDTYKMPVNITRCSNNYGPYQFPEKLIPLIINNCLNNNPIPIYGDGLNVRDWLYVEDHCKAIDMVIN
KGSLGEVYNIGGHNEKTNIFIVKAIIEYINENVDKTVTEKLIKYVEDRKGHDRRYGIDPTKIKEDLGWEPKTKFEDGIIK
TIEWYLSNKDWMENIITDNYQSYYKKMYGEN

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=44.6483180428135, Blast_Score=262, Evalue=3e-70,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=24.1176470588235, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI56237023, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI56118217, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI189083684, Length=330, Percent_Identity=24.2424242424242, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504031, Length=336, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=49.002849002849, Blast_Score=343, Evalue=7e-96,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=48.7323943661972, Blast_Score=343, Evalue=8e-96,
Organism=Escherichia coli, GI1786974, Length=348, Percent_Identity=26.1494252873563, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788366, Length=367, Percent_Identity=23.9782016348774, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=325, Percent_Identity=39.6923076923077, Blast_Score=235, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=34.2424242424242, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17539532, Length=336, Percent_Identity=26.1904761904762, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71982035, Length=357, Percent_Identity=26.3305322128852, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=359, Percent_Identity=26.1838440111421, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17507723, Length=338, Percent_Identity=26.6272189349112, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=251, Percent_Identity=25.8964143426295, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=251, Percent_Identity=25.8964143426295, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=25.6338028169014, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=26.5861027190332, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19923002, Length=352, Percent_Identity=30.9659090909091, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40461; Mature: 40461

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTYLVTGGAGFIGSNFILYMLDKYKNIKIINLDKLTYAGNLENLKEVESNINYSFVNGD
CCEEEEECCCCCHHCCEEEEEEECCCCEEEEEECCEEECCCHHHHHHHHHCCCEEEECCC
ICDRELVERVFKENNIDYVVNFAAESHVDRSIKDPEIFAKTNVLGTVNLLNVAKISWQTE
CCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCHHEEECCCEEEEEHEEEEEEECCCH
EAWVENKKFLQVSTDEVYGSLGDTGYFMEDTPLDPHSPYSSSKASGDLMVKAYFDTYKMP
HHHHCCCEEEEEEHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VNITRCSNNYGPYQFPEKLIPLIINNCLNNNPIPIYGDGLNVRDWLYVEDHCKAIDMVIN
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHEEHHHCC
KGSLGEVYNIGGHNEKTNIFIVKAIIEYINENVDKTVTEKLIKYVEDRKGHDRRYGIDPT
CCCCCCEEECCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
KIKEDLGWEPKTKFEDGIIKTIEWYLSNKDWMENIITDNYQSYYKKMYGEN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKTYLVTGGAGFIGSNFILYMLDKYKNIKIINLDKLTYAGNLENLKEVESNINYSFVNGD
CCEEEEECCCCCHHCCEEEEEEECCCCEEEEEECCEEECCCHHHHHHHHHCCCEEEECCC
ICDRELVERVFKENNIDYVVNFAAESHVDRSIKDPEIFAKTNVLGTVNLLNVAKISWQTE
CCCHHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCHHEEECCCEEEEEHEEEEEEECCCH
EAWVENKKFLQVSTDEVYGSLGDTGYFMEDTPLDPHSPYSSSKASGDLMVKAYFDTYKMP
HHHHCCCEEEEEEHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
VNITRCSNNYGPYQFPEKLIPLIINNCLNNNPIPIYGDGLNVRDWLYVEDHCKAIDMVIN
EEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEHHHHHEEHHHCC
KGSLGEVYNIGGHNEKTNIFIVKAIIEYINENVDKTVTEKLIKYVEDRKGHDRRYGIDPT
CCCCCCEEECCCCCCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCHH
KIKEDLGWEPKTKFEDGIIKTIEWYLSNKDWMENIITDNYQSYYKKMYGEN
HHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]