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The map label for this gene is gcvH

Identifier: 170754700

GI number: 170754700

Start: 818935

End: 819327

Strand: Direct

Name: gcvH

Synonym: CLD_0069

Alternate gene names: 170754700

Gene position: 818935-819327 (Clockwise)

Preceding gene: 170754781

Following gene: 170757782

Centisome position: 20.69

GC content: 33.59

Gene sequence:

>393_bases
ATGAAGGTTTTAAATAATTTACTTTACACTGGTGATCATGAATGGGTAAGGGTAGAAGATAACAAAGCATATATAGGAAT
CAGCGATTGTGCACAACGTATGTTAAGTGATATAGTTTTTGTTGAATTACCAGAGGTTGATGATGAAATAGCAAAAGGTG
AAACTTTTGCAACTATAGAATCTGTTAAAGCAGCATCAGACAGCTACATGCCTGTTAGTGGAACAATTGTAGAAATTAAT
GAAGAACTAGAGGATAATCCAGCAGCATTAAACGAAGATCCATATGGAAGCTGGATTGCTGCAATAGAGATGTCAGATAA
AAATGAATTAGAAGAATTAATAAAACCAGAAGTTTATGAGAAAATATGTGAAGAGTTAGACAAGGAGGCATAA

Upstream 100 bases:

>100_bases
CATAACCGCTTTTTACTACCGCTTTAAAAAAAGATGGGAGTATTAGAGTGGATAGCAATGAAATAAATATATAATGATTG
AAAAATTGGGGGTTAGTAGA

Downstream 100 bases:

>100_bases
TTATGTTTCCATATATTCCAAACACAGAAAAAGAAACAAAAGAAATTTTAGATTTTCTTTCAATAAAATCTGTAGATGAA
TTATTCAGTGATATACCAGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 130; Mature: 130

Protein sequence:

>130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKNELEELIKPEVYEKICEELDKEA

Sequences:

>Translated_130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKNELEELIKPEVYEKICEELDKEA
>Mature_130_residues
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIESVKAASDSYMPVSGTIVEIN
EELEDNPAALNEDPYGSWIAAIEMSDKNELEELIKPEVYEKICEELDKEA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=114, Percent_Identity=43.859649122807, Blast_Score=101, Evalue=2e-22,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=42.9824561403509, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=47.9338842975207, Blast_Score=112, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=45.0819672131148, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=43.1034482758621, Blast_Score=105, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=120, Percent_Identity=44.1666666666667, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=42.1052631578947, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14632; Mature: 14632

Theoretical pI: Translated: 3.77; Mature: 3.77

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHHEEEECCCCCHHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIAAIEMSDKNELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC
>Mature Secondary Structure
MKVLNNLLYTGDHEWVRVEDNKAYIGISDCAQRMLSDIVFVELPEVDDEIAKGETFATIE
CCHHHCCEECCCCCEEEEECCCEEEEHHHHHHHHHHHHEEEECCCCCHHHHCCCHHHHHH
SVKAASDSYMPVSGTIVEINEELEDNPAALNEDPYGSWIAAIEMSDKNELEELIKPEVYE
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHCHHHHH
KICEELDKEA
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA