| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is hypF
Identifier: 170754300
GI number: 170754300
Start: 1957519
End: 1959957
Strand: Reverse
Name: hypF
Synonym: CLD_2804
Alternate gene names: 170754300
Gene position: 1959957-1957519 (Counterclockwise)
Preceding gene: 170755019
Following gene: 170756113
Centisome position: 49.52
GC content: 26.45
Gene sequence:
>2439_bases ATGTATCTGATTGCTAAAGACAAATTCAATAATAAAAAGCGATATTTAATAAAAATAGAAGGTATAGTACAAGGGGTAGG ATTTAGACCTTTTGTATATAATAGAGCAACAGAATTTAATTTAAAAGGATATGTAAAAAATAAAGGTGGCATGGTAGAAG TAGATCTAGAAGGATATAAAGAAAATATTAAAAGCTTTATACTAGAGTTAATTAAGAGACCTCCTATTATGGCTAAAATA GAAGATTTAAAATGTAAATTTTTAAAACCTATCGATTATAAAGATTTTACTATTAAAAAAAGTTCTAATGAAAAAAATTC AGTAAAATTTATAAGCCCAGATGTAGCCCTTTGCAAAAACTGTATAAAAGAATTAAATTTAAGTACTAACAAAAGATATG AATATGGCTTTATTAATTGTACAGATTGTGGCCCTAGATACTCTATTATAAAATCATTACCTTATGATAGAGAAAATACA ACTATGAAAAAATTTACTATGTGTAATAGTTGTAAAGAAGAATATAATATTCCAGACAATAGACGATTTCATGCAGAGCC TAATTGTTGTAGTTTATGTGGCCCTTCATTAATATTATTGGACAATAAGAAACAAGTTATATGTATTAATAGCAAAAAGG AATATATAAATAATACACCTAATAATAATGATTATAATGAAAACAAAATTCATGAAAGTTATATAAATGTAGCTAATTTA AGTTATAAAAAACATCCCATTTATACTGCTGTAGAACTAATAAAAAAAGGATACATTGTAGCTGTAAAAGGAATAGGAGG ATTTCATTTAGTTTGTAATGGTAAAGATAAAAAAGCTATAGAAAATTTAAGAATAAGAAAAGAAAGACCTCATAAACCTT TAGCTATAATGTGTAAAGATTTAAAAATCGTAAAAGAATTGTGCTATGTATCTAAATTAGAGGAACAGATTTTAGAGGGT ACTAAAAAGCCTATTTTGATTTTAAAGAAAAAAGCTATAGATATATTGCCAGATAATATATCACCTTTCAATAAACATTT AGGAGTGATGCTACCTTATAGCCCCCTACATTATTTACTATTTAAAGAAGGTTTAGATATAATAATAGCTACCAGTGGTA ATGTAAGTGGTAGACCTATGGAATACAAAAATTGTGAAGCTATAGAAAATTTGAAAACTATAGCAGATTATTTTTTAATC CATAATAGAGATATTCATACTCCCATTGACGATTCTGTAGTAAAAGTTATAAATAAAAAAGAAAGTTTAATTAGAATAGG TCGTGGATATGCCCCTTATACTTTAAATATAAAAACTAAAAATGAATTATTAGCAAAGGGTGCAGAAGAAAAAAACACCT TTTGCCTGTCTCAAAAGGGATATGTGTGCATGAGTCAATATATAGGAGATCTAAAAAATATAGATTGCTACAATAGACAT TTAAATACTATAGAACACCTACAAACTTTATTAGATACAAATCCTAAAATATCTATAGAAGATTTGCACCCAAATTTTAA TACTAATTATGAAAATAAAAACTATGATAGAACCATTAAAGTTCAACATCATCATGCCCATATGGTAAGTTGTATGGTAG AGCATTCGTTATTTGATAGGGTTATTGCTGTGGTATTTGATGGTACAGGCTTTGGCAAAGACAATAGTATATGGGGGGGA GAGTTTTTAATAGGTGACAGAACCTATCTTGAAAGAGTTGGACATTTAAAAAAAGTTAAAATCCAGGGAGGAGATAAATC AATAGAAGAACCATGGAGATGTGCCTTAAGCTATTTGTATTCTATAAATTATGAAGAGAGCTCTATAATTAAAAATGTAG AAGAAGAAAAAATTAAGATGGTAAAGAAAGCTTTATACAATAATATAAATTGCTATACATCTTCTAGTATGGGAAGGTTT TTTGATGCAGTAACTTCATTAATAGGCATTAGAAATATAATAAGTTATGATGGTCAAGCTCCTATAGAGTTAGAAAATAT CATAGATGAACCTATATCTCTTTATTATAATTATAGTATTAATAAAATAGATAATGTTTTTCAAATAGATTATAAAGATA TAATTTTAGGAATAATTAAAGATTTAAAAGAAAATAAAGATTTATCTACCATATCATCAAAGTTTCATAATACAATTATT GATTTTACTTGTAACTTGGTGTGTAAAATAAAAAAAACTTATGAAATGAAATGTAAATTAGATAACGTTGTATTAAGTGG AGGAGTTTTTCAAAATAATTACTTATTAAAAGGTGTATACAGAAACTTAAAAGATAATGGATTTAAAGTGTTTTTCAATG AAAAAGTTCCTATAAATGATGAAGGAATAAGCCTTGGTCAATTAGCTATTGGTGACTCTATTATAGAAAGGGAGAAAAGG AATGTGCATTGCAATACCGGGGAAAATTGTAAGTATTGA
Upstream 100 bases:
>100_bases ACAAAATACAATAATAATTTAGTAGATGAAAATATAGAAATTGTTGTAGAAAGACAACCTATAGATGAGCAAACAGCTAT TATTAAATTATTAGAAGGTG
Downstream 100 bases:
>100_bases TGGCTACTATGCTATAACAAATACTATGGATATAGAAAGTGAAATAAATATATCACTTATAGAAAATCCATCTATCAATG ACTATGTTCTAATTCATGCA
Product: [NiFe] hydrogenase maturation protein HypF
Products: NA
Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]
Number of amino acids: Translated: 812; Mature: 812
Protein sequence:
>812_residues MYLIAKDKFNNKKRYLIKIEGIVQGVGFRPFVYNRATEFNLKGYVKNKGGMVEVDLEGYKENIKSFILELIKRPPIMAKI EDLKCKFLKPIDYKDFTIKKSSNEKNSVKFISPDVALCKNCIKELNLSTNKRYEYGFINCTDCGPRYSIIKSLPYDRENT TMKKFTMCNSCKEEYNIPDNRRFHAEPNCCSLCGPSLILLDNKKQVICINSKKEYINNTPNNNDYNENKIHESYINVANL SYKKHPIYTAVELIKKGYIVAVKGIGGFHLVCNGKDKKAIENLRIRKERPHKPLAIMCKDLKIVKELCYVSKLEEQILEG TKKPILILKKKAIDILPDNISPFNKHLGVMLPYSPLHYLLFKEGLDIIIATSGNVSGRPMEYKNCEAIENLKTIADYFLI HNRDIHTPIDDSVVKVINKKESLIRIGRGYAPYTLNIKTKNELLAKGAEEKNTFCLSQKGYVCMSQYIGDLKNIDCYNRH LNTIEHLQTLLDTNPKISIEDLHPNFNTNYENKNYDRTIKVQHHHAHMVSCMVEHSLFDRVIAVVFDGTGFGKDNSIWGG EFLIGDRTYLERVGHLKKVKIQGGDKSIEEPWRCALSYLYSINYEESSIIKNVEEEKIKMVKKALYNNINCYTSSSMGRF FDAVTSLIGIRNIISYDGQAPIELENIIDEPISLYYNYSINKIDNVFQIDYKDIILGIIKDLKENKDLSTISSKFHNTII DFTCNLVCKIKKTYEMKCKLDNVVLSGGVFQNNYLLKGVYRNLKDNGFKVFFNEKVPINDEGISLGQLAIGDSIIEREKR NVHCNTGENCKY
Sequences:
>Translated_812_residues MYLIAKDKFNNKKRYLIKIEGIVQGVGFRPFVYNRATEFNLKGYVKNKGGMVEVDLEGYKENIKSFILELIKRPPIMAKI EDLKCKFLKPIDYKDFTIKKSSNEKNSVKFISPDVALCKNCIKELNLSTNKRYEYGFINCTDCGPRYSIIKSLPYDRENT TMKKFTMCNSCKEEYNIPDNRRFHAEPNCCSLCGPSLILLDNKKQVICINSKKEYINNTPNNNDYNENKIHESYINVANL SYKKHPIYTAVELIKKGYIVAVKGIGGFHLVCNGKDKKAIENLRIRKERPHKPLAIMCKDLKIVKELCYVSKLEEQILEG TKKPILILKKKAIDILPDNISPFNKHLGVMLPYSPLHYLLFKEGLDIIIATSGNVSGRPMEYKNCEAIENLKTIADYFLI HNRDIHTPIDDSVVKVINKKESLIRIGRGYAPYTLNIKTKNELLAKGAEEKNTFCLSQKGYVCMSQYIGDLKNIDCYNRH LNTIEHLQTLLDTNPKISIEDLHPNFNTNYENKNYDRTIKVQHHHAHMVSCMVEHSLFDRVIAVVFDGTGFGKDNSIWGG EFLIGDRTYLERVGHLKKVKIQGGDKSIEEPWRCALSYLYSINYEESSIIKNVEEEKIKMVKKALYNNINCYTSSSMGRF FDAVTSLIGIRNIISYDGQAPIELENIIDEPISLYYNYSINKIDNVFQIDYKDIILGIIKDLKENKDLSTISSKFHNTII DFTCNLVCKIKKTYEMKCKLDNVVLSGGVFQNNYLLKGVYRNLKDNGFKVFFNEKVPINDEGISLGQLAIGDSIIEREKR NVHCNTGENCKY >Mature_812_residues MYLIAKDKFNNKKRYLIKIEGIVQGVGFRPFVYNRATEFNLKGYVKNKGGMVEVDLEGYKENIKSFILELIKRPPIMAKI EDLKCKFLKPIDYKDFTIKKSSNEKNSVKFISPDVALCKNCIKELNLSTNKRYEYGFINCTDCGPRYSIIKSLPYDRENT TMKKFTMCNSCKEEYNIPDNRRFHAEPNCCSLCGPSLILLDNKKQVICINSKKEYINNTPNNNDYNENKIHESYINVANL SYKKHPIYTAVELIKKGYIVAVKGIGGFHLVCNGKDKKAIENLRIRKERPHKPLAIMCKDLKIVKELCYVSKLEEQILEG TKKPILILKKKAIDILPDNISPFNKHLGVMLPYSPLHYLLFKEGLDIIIATSGNVSGRPMEYKNCEAIENLKTIADYFLI HNRDIHTPIDDSVVKVINKKESLIRIGRGYAPYTLNIKTKNELLAKGAEEKNTFCLSQKGYVCMSQYIGDLKNIDCYNRH LNTIEHLQTLLDTNPKISIEDLHPNFNTNYENKNYDRTIKVQHHHAHMVSCMVEHSLFDRVIAVVFDGTGFGKDNSIWGG EFLIGDRTYLERVGHLKKVKIQGGDKSIEEPWRCALSYLYSINYEESSIIKNVEEEKIKMVKKALYNNINCYTSSSMGRF FDAVTSLIGIRNIISYDGQAPIELENIIDEPISLYYNYSINKIDNVFQIDYKDIILGIIKDLKENKDLSTISSKFHNTII DFTCNLVCKIKKTYEMKCKLDNVVLSGGVFQNNYLLKGVYRNLKDNGFKVFFNEKVPINDEGISLGQLAIGDSIIEREKR NVHCNTGENCKY
Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb
COG id: COG0068
COG function: function code O; Hydrogenase maturation factor
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 YrdC-like domain [H]
Homologues:
Organism=Escherichia coli, GI2367152, Length=789, Percent_Identity=33.0798479087452, Blast_Score=410, Evalue=1e-115,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001792 - InterPro: IPR017968 - InterPro: IPR004421 - InterPro: IPR017945 - InterPro: IPR006070 - InterPro: IPR011125 [H]
Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]
EC number: NA
Molecular weight: Translated: 93361; Mature: 93361
Theoretical pI: Translated: 8.90; Mature: 8.90
Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
3.2 %Cys (Translated Protein) 1.7 %Met (Translated Protein) 4.9 %Cys+Met (Translated Protein) 3.2 %Cys (Mature Protein) 1.7 %Met (Mature Protein) 4.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYLIAKDKFNNKKRYLIKIEGIVQGVGFRPFVYNRATEFNLKGYVKNKGGMVEVDLEGYK CEEEEECCCCCCCEEEEEEEEECCCCCCCCEEECCCEEEEEEEEEECCCCEEEEEHHHHH ENIKSFILELIKRPPIMAKIEDLKCKFLKPIDYKDFTIKKSSNEKNSVKFISPDVALCKN HHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCCCEEEEECCCCCCCEEEECCCHHHHHH CIKELNLSTNKRYEYGFINCTDCGPRYSIIKSLPYDRENTTMKKFTMCNSCKEEYNIPDN HHHHCCCCCCCEEEECEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC RRFHAEPNCCSLCGPSLILLDNKKQVICINSKKEYINNTPNNNDYNENKIHESYINVANL CEEECCCCHHHHCCCEEEEEECCCEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHC SYKKHPIYTAVELIKKGYIVAVKGIGGFHLVCNGKDKKAIENLRIRKERPHKPLAIMCKD CCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHH LKIVKELCYVSKLEEQILEGTKKPILILKKKAIDILPDNISPFNKHLGVMLPYSPLHYLL HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEECCCCCCCHHHCCCEEECCCHHHHHH FKEGLDIIIATSGNVSGRPMEYKNCEAIENLKTIADYFLIHNRDIHTPIDDSVVKVINKK HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCH ESLIRIGRGYAPYTLNIKTKNELLAKGAEEKNTFCLSQKGYVCMSQYIGDLKNIDCYNRH HHHHHHCCCCCCEEEEEECHHHHHHCCCCCCCCEEECCCCCCHHHHHHCCCCCCCHHHHH LNTIEHLQTLLDTNPKISIEDLHPNFNTNYENKNYDRTIKVQHHHAHMVSCMVEHSLFDR HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH VIAVVFDGTGFGKDNSIWGGEFLIGDRTYLERVGHLKKVKIQGGDKSIEEPWRCALSYLY EEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHH SINYEESSIIKNVEEEKIKMVKKALYNNINCYTSSSMGRFFDAVTSLIGIRNIISYDGQA HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCC PIELENIIDEPISLYYNYSINKIDNVFQIDYKDIILGIIKDLKENKDLSTISSKFHNTII CEEHHHHHCCCEEEEEECCHHCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH DFTCNLVCKIKKTYEMKCKLDNVVLSGGVFQNNYLLKGVYRNLKDNGFKVFFNEKVPIND EEEEEEEEEEECCEEEEEEECCEEEECCEEECCCHHHHHHHCCCCCCEEEEECCCCCCCC EGISLGQLAIGDSIIEREKRNVHCNTGENCKY CCCEECHHHHCHHHHHHCCCCEECCCCCCCCC >Mature Secondary Structure MYLIAKDKFNNKKRYLIKIEGIVQGVGFRPFVYNRATEFNLKGYVKNKGGMVEVDLEGYK CEEEEECCCCCCCEEEEEEEEECCCCCCCCEEECCCEEEEEEEEEECCCCEEEEEHHHHH ENIKSFILELIKRPPIMAKIEDLKCKFLKPIDYKDFTIKKSSNEKNSVKFISPDVALCKN HHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCCCEEEEECCCCCCCEEEECCCHHHHHH CIKELNLSTNKRYEYGFINCTDCGPRYSIIKSLPYDRENTTMKKFTMCNSCKEEYNIPDN HHHHCCCCCCCEEEECEEEECCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCC RRFHAEPNCCSLCGPSLILLDNKKQVICINSKKEYINNTPNNNDYNENKIHESYINVANL CEEECCCCHHHHCCCEEEEEECCCEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHC SYKKHPIYTAVELIKKGYIVAVKGIGGFHLVCNGKDKKAIENLRIRKERPHKPLAIMCKD CCCCCCHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHH LKIVKELCYVSKLEEQILEGTKKPILILKKKAIDILPDNISPFNKHLGVMLPYSPLHYLL HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECHHEECCCCCCCHHHCCCEEECCCHHHHHH FKEGLDIIIATSGNVSGRPMEYKNCEAIENLKTIADYFLIHNRDIHTPIDDSVVKVINKK HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCH ESLIRIGRGYAPYTLNIKTKNELLAKGAEEKNTFCLSQKGYVCMSQYIGDLKNIDCYNRH HHHHHHCCCCCCEEEEEECHHHHHHCCCCCCCCEEECCCCCCHHHHHHCCCCCCCHHHHH LNTIEHLQTLLDTNPKISIEDLHPNFNTNYENKNYDRTIKVQHHHAHMVSCMVEHSLFDR HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHH VIAVVFDGTGFGKDNSIWGGEFLIGDRTYLERVGHLKKVKIQGGDKSIEEPWRCALSYLY EEEEEECCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHH SINYEESSIIKNVEEEKIKMVKKALYNNINCYTSSSMGRFFDAVTSLIGIRNIISYDGQA HCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCC PIELENIIDEPISLYYNYSINKIDNVFQIDYKDIILGIIKDLKENKDLSTISSKFHNTII CEEHHHHHCCCEEEEEECCHHCCCCEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH DFTCNLVCKIKKTYEMKCKLDNVVLSGGVFQNNYLLKGVYRNLKDNGFKVFFNEKVPIND EEEEEEEEEEECCEEEEEEECCEEEECCEEECCCHHHHHHHCCCCCCEEEEECCCCCCCC EGISLGQLAIGDSIIEREKRNVHCNTGENCKY CCCEECHHHHCHHHHHHCCCCEECCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8590279; 8905231 [H]