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The map label for this gene is gatB

Identifier: 170754279

GI number: 170754279

Start: 3530282

End: 3531712

Strand: Reverse

Name: gatB

Synonym: CLD_1257

Alternate gene names: 170754279

Gene position: 3531712-3530282 (Counterclockwise)

Preceding gene: 170757862

Following gene: 170754890

Centisome position: 89.22

GC content: 29.77

Gene sequence:

>1431_bases
ATGGATTTTGAAGCAGTTATAGGATTGGAAGTTCATGCTGAACTTTCAACAAATACTAAAATATATTGTGGATGCACTAC
AGAATTTGGTGGTCAACCTAATACTCATGTTTGTCCAATATGTTTAGGATTACCAGGTTCTCTACCTCAATTAAATAAAA
GGGTAGTAGAGTATGGAATTAAGGCAGGATTAGCTTTAAATTGTTCTATAAACAAAGTTTGTAGAATGGATAGAAAAAAT
TATTTTTATCCAGATTGCCCTAAAAATTATCAAATAACTCAAGATGAAGTTCCAATATGTAGAGATGGATATATTGAAAT
AGAGTTAGAAAACGGTGAAAAGAAAAAAATAGGCATAGAAAGAATACATATGGAAGAAGATGCTGGAAAATTACTTCATA
CTAATGCGGGAACATTGGTAGATTACAATAGAGCAGGAGTACCATTAATAGAGATAGTATCAAGACCAGATATAAGAACT
CCAGAAGAAGCTACAAAATACTTAGAGAAATTAAAGAGTATACTATCTTCAATAGAAGTATCAGATTGTAAAATGGAGCA
AGGTTCCTTAAGATGTGATGGTAATATATCTGTAATGCCTAAAGGTAGCGAAAAATTTGGAGTAAGATCAGAAATAAAAA
ATATGAATTCTTTTAAAGCTTTGGAAAAAGCTTTATCATATGAATATGATAGACATGTAGAAGCTGTAACAAAGGGAGAA
ATTTTAGAGCAAGAAACAAGAAGATGGGACGAAGCTAATTCTGTTACAGTGCTTATGCGAAGCAAAGAAAAAGCTAATGA
TTATAGATACTTTCCAGAAGGAGATTTAGTTACATTAAATATTTCAGATGAATGGATAGAAGAAGTAAGAAAAACTATAC
CAGAGTTACCACATGAAAAAGCTGAAAGATTTGTAAATGAATTTGGAATACCTAAATATGATGCTATGGTATTAACTTTA
ACAATGGATATGGCTAAATTTTTTGAAGAAACTGCCCTAAAGAGTGAAGATGCAAAAGTTGCTTCTAACTGGTTAATGGG
TGATATTTCAAGACTTATGAATGAAAAAACTATAGAAGTTAAAGATTTGAAATTTAATCCTGAACAATTAGCAGAGTTAA
TTAAATTAATAAACGCTGGAACTATATCTAATAATATAGGTAAAAAAGTTTTAGATGATATGTTTAAGTCAGGTAAAAAT
CCTAAAGATATAGTTGAAGAAAAAGGTTTGGTTCAAAATAATGATGAAGGTGCTATATTAGAAGTAGTTAAAAATATTAT
AGAGAATAATCCTCAATCTATAGAAGACTTCAAAAATGGAAAGAAAAGAGCCTTAGGCTTCCTTGTAGGCCTTGTAATGA
AAGAAACAAAAGGAAAAGCTAATCCTCAAATAGTAAATAAATTAGTAAGTGAAGAAGCAAATAAAATGTAA

Upstream 100 bases:

>100_bases
TATTTAATTTAGCATATAGCTATGAACAATCAGTAGATTTTTATAAAATGAGAGCGGATTTTTAAGTATATAGTTAAAAA
CAAAGGAGTGAAAAACATAG

Downstream 100 bases:

>100_bases
ATATTAGAATAAAAGATCCTCACATAAAAATGTGGGGATTTTTTTTATCCGATGACTACCCGCTCTAATACTCCCATCTT
CTTCAAAGTGGGAGTAAAGA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKVASNWLMGDISRLMNEKTIEVKDLKFNPEQLAELIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM

Sequences:

>Translated_476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKVASNWLMGDISRLMNEKTIEVKDLKFNPEQLAELIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
>Mature_476_residues
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGIKAGLALNCSINKVCRMDRKN
YFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIERIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRT
PEEATKYLEKLKSILSSIEVSDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEKAERFVNEFGIPKYDAMVLTL
TMDMAKFFEETALKSEDAKVASNWLMGDISRLMNEKTIEVKDLKFNPEQLAELIKLINAGTISNNIGKKVLDDMFKSGKN
PKDIVEEKGLVQNNDEGAILEVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=491, Percent_Identity=37.0672097759674, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI115534094, Length=298, Percent_Identity=37.248322147651, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6319391, Length=500, Percent_Identity=29.4, Blast_Score=221, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550808, Length=487, Percent_Identity=34.0862422997947, Blast_Score=283, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53716; Mature: 53716

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGI
CCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KAGLALNCSINKVCRMDRKNYFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIE
HCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRTPEEATKYLEKLKSILSSIEV
HEEEHHCCCCEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
CCCCCCCCCEEECCCEEEECCCCHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEK
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
AERFVNEFGIPKYDAMVLTLTMDMAKFFEETALKSEDAKVASNWLMGDISRLMNEKTIEV
HHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEE
KDLKFNPEQLAELIKLINAGTISNNIGKKVLDDMFKSGKNPKDIVEEKGLVQNNDEGAIL
EECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHH
EVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
HHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFEAVIGLEVHAELSTNTKIYCGCTTEFGGQPNTHVCPICLGLPGSLPQLNKRVVEYGI
CCCCEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHH
KAGLALNCSINKVCRMDRKNYFYPDCPKNYQITQDEVPICRDGYIEIELENGEKKKIGIE
HCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHH
RIHMEEDAGKLLHTNAGTLVDYNRAGVPLIEIVSRPDIRTPEEATKYLEKLKSILSSIEV
HEEEHHCCCCEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
SDCKMEQGSLRCDGNISVMPKGSEKFGVRSEIKNMNSFKALEKALSYEYDRHVEAVTKGE
CCCCCCCCCEEECCCEEEECCCCHHCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHH
ILEQETRRWDEANSVTVLMRSKEKANDYRYFPEGDLVTLNISDEWIEEVRKTIPELPHEK
HHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHH
AERFVNEFGIPKYDAMVLTLTMDMAKFFEETALKSEDAKVASNWLMGDISRLMNEKTIEV
HHHHHHHCCCCCHHHEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEE
KDLKFNPEQLAELIKLINAGTISNNIGKKVLDDMFKSGKNPKDIVEEKGLVQNNDEGAIL
EECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHH
EVVKNIIENNPQSIEDFKNGKKRALGFLVGLVMKETKGKANPQIVNKLVSEEANKM
HHHHHHHCCCCCHHHHHHCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA