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The map label for this gene is gatA

Identifier: 170757862

GI number: 170757862

Start: 3531748

End: 3533205

Strand: Reverse

Name: gatA

Synonym: CLD_1256

Alternate gene names: 170757862

Gene position: 3533205-3531748 (Counterclockwise)

Preceding gene: 170756377

Following gene: 170754279

Centisome position: 89.26

GC content: 31.28

Gene sequence:

>1458_bases
ATGGATTTAACTAAATTAACAGCACACGAATTAAAAGATATGCTTTCAAATAAAGAAGTTAAAGCAGAAGAAATAACAAA
AGCTTTTTTAGATAGAATAAATTTAGTAGACAATAAGCTAGGAGCATACTTATATGTATCAGAGGAAGAAGCTATAAAGA
AAGCTAAAGAAATTGATGTAAAAATAGAAAAAAATGAAGAGTTAAAAGCCTTATCAGGTATACCAATAGGTATAAAAGAT
AATATAAATGTAAAAGGAATGCAAAATACTTGTGCATCTAAAATATTAGAAGGTTATACTTCACCTTATGATGCTCATGT
AACAGAAAAAATAAAACAAGAAGAAGGAATAATCCTTGGCAAATTAAACATGGATGAGTTTGCTATGGGATCATCTACAG
AAAATAGTGCATTTAAATTAGCCAAAAATCCATGGGATTTAGAAAGAGTACCAGGTGGATCTTCAGGTGGATCTGCAGTG
GCGGTAGCAGGATCTGAAGCTACTTTATCATTAGGAACAGATACTGGTGGTTCTGTAAGACAACCTGCATCCTTTTGTGG
AGTAGTTGGTTTAAAACCTACATATGGAAGAATATCAAGATCAGGGGTTGTAGCTTTTGGTTCTACATTAGATCAAGTAG
GTCCAATGGGAAAAGATGTAGAGGATTGTGCTTTGTTAACATCAGTTATAGCAGGATTAGATAAAAAGGATTTTACAACA
GTAGATAAGGAAGTTCCTGATTATAAAAAAAGTTTAACTAAAGATATAAAGGGTAAAAGAATTGGTATTCCAAAGGAGTT
TTTTGGAGATGGATTAGATAAGAATATAAGAAAATCTGTAGAAGAAGCTATAAAGGTATTAGAGGCAAATGGAGCAGAAG
TTAAGCCATGTTCTCTACCATTAATGGATTATGCCCTATCAGCTTATTACATAATATCTAGTGCAGAAGCTTCATCAAAT
TTAGCTAGATTTGACGGTATAAGATATGGTTATAGATCAAAAAACTTTAAAGATGCTCAGGATATATATTTAAAATCTAG
AAGTGAAGGCTTTGGAGATGAAGTTAAGAGAAGAATAATGCTTGGAACTTATGTATTATCAGCAGGATACTATGATGCTT
ATTATAAAAAAGCATTAAAGGTAAGAAAACTTATAAAGGATGATTTCCAAAGAGTATTTAAAGACTTTGATGCTATAGTA
TCACCAACATCTCCAACTACAGCATTTAAAGTAGGAGAAAAGAAAGACGATGTAATGTCAATGTATCTTTCAGATATATA
TACTGTTCCAATTAGTGTAGCAGGAGTACCAGCTATATCATTACCATGTGGTATGGTAGATGGACTTCCAGTAGGACTTC
AAATTATATCAGATTATTTTAAAGAAGATGTATTATTTAATTTAGCATATAGCTATGAACAATCAGTAGATTTTTATAAA
ATGAGAGCGGATTTTTAA

Upstream 100 bases:

>100_bases
AGTTATAGATAATGCACCAGAAAGCTTAGAAGAATATGTAATTGTTCCTAAAGTTATAGATTAATAATTCTTTTGTTTTC
TGGAAAAGGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
GTATATAGTTAAAAACAAAGGAGTGAAAAACATAGATGGATTTTGAAGCAGTTATAGGATTGGAAGTTCATGCTGAACTT
TCAACAAATACTAAAATATA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPIGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNIRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMVDGLPVGLQIISDYFKEDVLFNLAYSYEQSVDFYK
MRADF

Sequences:

>Translated_485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPIGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNIRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMVDGLPVGLQIISDYFKEDVLFNLAYSYEQSVDFYK
MRADF
>Mature_485_residues
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDVKIEKNEELKALSGIPIGIKD
NINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILGKLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAV
AVAGSEATLSLGTDTGGSVRQPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNIRKSVEEAIKVLEANGAEVKPCSLPLMDYALSAYYIISSAEASSN
LARFDGIRYGYRSKNFKDAQDIYLKSRSEGFGDEVKRRIMLGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIV
SPTSPTTAFKVGEKKDDVMSMYLSDIYTVPISVAGVPAISLPCGMVDGLPVGLQIISDYFKEDVLFNLAYSYEQSVDFYK
MRADF

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=465, Percent_Identity=36.1290322580645, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI195972892, Length=507, Percent_Identity=23.8658777120316, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI166795287, Length=291, Percent_Identity=27.1477663230241, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=435, Percent_Identity=37.7011494252874, Blast_Score=294, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17537465, Length=506, Percent_Identity=24.901185770751, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17556264, Length=293, Percent_Identity=28.3276450511945, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=318, Percent_Identity=28.9308176100629, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=313, Percent_Identity=27.1565495207668, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=304, Percent_Identity=27.6315789473684, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=304, Percent_Identity=28.2894736842105, Blast_Score=81, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323950, Length=415, Percent_Identity=37.5903614457831, Blast_Score=232, Evalue=8e-62,
Organism=Saccharomyces cerevisiae, GI6319685, Length=456, Percent_Identity=28.5087719298246, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320448, Length=539, Percent_Identity=24.6753246753247, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648113, Length=498, Percent_Identity=37.5502008032129, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24648435, Length=497, Percent_Identity=24.9496981891348, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24648437, Length=497, Percent_Identity=24.9496981891348, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550774, Length=496, Percent_Identity=25.2016129032258, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=24.2362525458248, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=24.2362525458248, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=24.2362525458248, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=24.2362525458248, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=24.7058823529412, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356731, Length=489, Percent_Identity=25.5623721881391, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648441, Length=430, Percent_Identity=24.8837209302326, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=430, Percent_Identity=24.8837209302326, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161078093, Length=512, Percent_Identity=24.21875, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53224; Mature: 53224

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCE
KIEKNEELKALSGIPIGIKDNINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILG
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGSEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNIRKSVEEAIKVLEANGAEVKPCSLP
HHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGYRSKNFKDAQDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMVDGLPVGLQIISDYFKEDVLFNLAYSYEQSVDFYK
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
MRADF
EECCC
>Mature Secondary Structure
MDLTKLTAHELKDMLSNKEVKAEEITKAFLDRINLVDNKLGAYLYVSEEEAIKKAKEIDV
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCE
KIEKNEELKALSGIPIGIKDNINVKGMQNTCASKILEGYTSPYDAHVTEKIKQEEGIILG
EEECCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEE
KLNMDEFAMGSSTENSAFKLAKNPWDLERVPGGSSGGSAVAVAGSEATLSLGTDTGGSVR
ECCCHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCC
QPASFCGVVGLKPTYGRISRSGVVAFGSTLDQVGPMGKDVEDCALLTSVIAGLDKKDFTT
CCHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
VDKEVPDYKKSLTKDIKGKRIGIPKEFFGDGLDKNIRKSVEEAIKVLEANGAEVKPCSLP
HHHCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCH
LMDYALSAYYIISSAEASSNLARFDGIRYGYRSKNFKDAQDIYLKSRSEGFGDEVKRRIM
HHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHH
LGTYVLSAGYYDAYYKKALKVRKLIKDDFQRVFKDFDAIVSPTSPTTAFKVGEKKDDVMS
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHH
MYLSDIYTVPISVAGVPAISLPCGMVDGLPVGLQIISDYFKEDVLFNLAYSYEQSVDFYK
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
MRADF
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA