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The map label for this gene is oppA [H]

Identifier: 170754890

GI number: 170754890

Start: 3528435

End: 3529997

Strand: Reverse

Name: oppA [H]

Synonym: CLD_1258

Alternate gene names: 170754890

Gene position: 3529997-3528435 (Counterclockwise)

Preceding gene: 170754279

Following gene: 170756402

Centisome position: 89.18

GC content: 24.7

Gene sequence:

>1563_bases
ATGAAAAAGATTATATGTTTTTTATTAAGTTTTACATTAATTTTTTTTAGCGGTTGTGTAGAAAAAAACAAAGAAGTCAC
CTCAGATGAGGGAATAAGAGATTACTTAATATATAACATAGAAACTATGCCTAAGGATTTAATTATGCTAGAAGACGGTT
CACAAAGAGAGGAGGACTTATTATTAAGTCTATTTGATGGATTGATAGGATTAGATAAAAGTAATAACATTGTTCCAGAG
TTGGCAGAAAAATGGGAAGTTAGTAAAGATGGTATAGATTATAAATTTTATATTAAGGATAATTTATTTTGGAGCAATGG
GAAGGAAATAGCAGCTAGGGATTTTGAAAAATTTTTTAAAGATATTCTTATATATTGTAAGGAAAATAATATAAGATTCA
ATGACCTAAATTCTGTATATGGAGTTAAGGACTACTTAGCTAATGGAAATCTAGATAACATAGCTATAAAAGCTAAAGAA
GATAATATATTAGAAATAAGACTAAATAATAAAGATCCATATTTTTTAAGTACTCTTTCTAAGCCTAAATATAAATTAAG
AAAAATAGATAAGAAAAACAAAAATTATAAAAAAACTTTTAATGAAATACCATATACAGGAGCATTTTGTATAAATAAAA
TAGAAAAAGACCAAATAACAATTAACAAAAATAATAGATATTGGGATAAAGAAAATATAAAGATTAATAGGATAGTACTT
AAATCTATACAAAACAGTGAAGAAGCATTGGCTCATTTAAAGACTTCCAAATTAGACTTGTTTATGAATCCTCCTATAGC
TGAAATTAATAATTTAAATAAAGAAGGATTAATATCTAAAAAGTTATCTTTAGAATCAGGGAATATAATATTCAATAAAA
AAGATTATATAGATCTAAACTTAAGAAGGGCAATAGATAGCTGTATTAGTAGGGAGGATATGTGTTCGAAGTTACTATAT
GGGAAATCAGTGCCAGCTATTGCTTATGTACCAAATAATATAGCAGAAAATGATGATATAAGTTATGGTAAAAATTACTT
TAGTTTAAATAAAAATTTACAAAGGGCAAAAGAATTTTTAGATAAAAGTGAATATAAAAAAGAAAAGAAGGATTTAAAAA
TTATATCAATAGAAGATCCTATTTCTAATAAAATAGCTAATAGTATAATAGGGGAATTAAAAGAGAATTTAGATATAAAT
CTTAATTTGGTTAATTGCAAGGATGAGAATGAATTAGAACAGAAAATTAAAAATGGGGATTATGACTTAGCTTTTGTTAG
AAATAAAGCTAAAAATAACGATCCTTTATTTTTCCTCTTAAACTTTACTAGTGATTCTTTGCAATATCAAGATTTTTTAA
ATAAAATTAAATTAGAAGATAATATGTGGAAAAAGAGAGAATATATCAATAAAGCACAAGATGTTCTGGCTGAGGATTTG
TATACAATACCTGTTTGTTTTTTTTATGATATGTTATGTAAAAGAAGTAGGGTACAAGGGCAATATATTACCATAGATGG
TAATATTATTATAAAGAAAATAACCTTAGCACCCTATAATTAA

Upstream 100 bases:

>100_bases
TAGTTTGAATTATATGTAAAGTTTAAAAATATTGTTTATTTTAACCTATAAGTGTAAATAATTAAATATAGTATATGATT
CTTCAAAGCGGGGGGATAAA

Downstream 100 bases:

>100_bases
TATTATTTTATAGTGGTTTTATTTTTAGATGATTTAATAGGAAGAAAATAAGCACAATTAGCATTTTCTTCGCGAATACT
TAAAAAGGGACTGATATAAT

Product: solute-binding family 5 protein

Products: NA

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREEDLLLSLFDGLIGLDKSNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIAARDFEKFFKDILIYCKENNIRFNDLNSVYGVKDYLANGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKTFNEIPYTGAFCINKIEKDQITINKNNRYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLESGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKENLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAEDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNIIIKKITLAPYN

Sequences:

>Translated_520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREEDLLLSLFDGLIGLDKSNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIAARDFEKFFKDILIYCKENNIRFNDLNSVYGVKDYLANGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKTFNEIPYTGAFCINKIEKDQITINKNNRYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLESGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKENLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAEDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNIIIKKITLAPYN
>Mature_520_residues
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREEDLLLSLFDGLIGLDKSNNIVPE
LAEKWEVSKDGIDYKFYIKDNLFWSNGKEIAARDFEKFFKDILIYCKENNIRFNDLNSVYGVKDYLANGNLDNIAIKAKE
DNILEIRLNNKDPYFLSTLSKPKYKLRKIDKKNKNYKKTFNEIPYTGAFCINKIEKDQITINKNNRYWDKENIKINRIVL
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLESGNIIFNKKDYIDLNLRRAIDSCISREDMCSKLLY
GKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFLDKSEYKKEKKDLKIISIEDPISNKIANSIIGELKENLDIN
LNLVNCKDENELEQKIKNGDYDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAEDL
YTIPVCFFYDMLCKRSRVQGQYITIDGNIIIKKITLAPYN

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789397, Length=515, Percent_Identity=23.3009708737864, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787495, Length=480, Percent_Identity=21.875, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081878, Length=506, Percent_Identity=21.3438735177866, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789966, Length=437, Percent_Identity=21.2814645308924, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60642; Mature: 60642

Theoretical pI: Translated: 8.07; Mature: 8.07

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREEDL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHH
LLSLFDGLIGLDKSNNIVPELAEKWEVSKDGIDYKFYIKDNLFWSNGKEIAARDFEKFFK
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHH
DILIYCKENNIRFNDLNSVYGVKDYLANGNLDNIAIKAKEDNILEIRLNNKDPYFLSTLS
HHEEEECCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCC
KPKYKLRKIDKKNKNYKKTFNEIPYTGAFCINKIEKDQITINKNNRYWDKENIKINRIVL
CCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCEECCCCCEEHHHHH
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLESGNIIFNKKDYIDLN
HHHCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCCEEHEEECCCCCEEECCCCEEEHH
LRRAIDSCISREDMCSKLLYGKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFL
HHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHH
DKSEYKKEKKDLKIISIEDPISNKIANSIIGELKENLDINLNLVNCKDENELEQKIKNGD
HHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCC
YDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAEDL
CEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTIPVCFFYDMLCKRSRVQGQYITIDGNIIIKKITLAPYN
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCC
>Mature Secondary Structure
MKKIICFLLSFTLIFFSGCVEKNKEVTSDEGIRDYLIYNIETMPKDLIMLEDGSQREEDL
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCEEEEECCCCCHHHHH
LLSLFDGLIGLDKSNNIVPELAEKWEVSKDGIDYKFYIKDNLFWSNGKEIAARDFEKFFK
HHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCEEECCCCHHHHHHHHHHHH
DILIYCKENNIRFNDLNSVYGVKDYLANGNLDNIAIKAKEDNILEIRLNNKDPYFLSTLS
HHEEEECCCCEEEECCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCC
KPKYKLRKIDKKNKNYKKTFNEIPYTGAFCINKIEKDQITINKNNRYWDKENIKINRIVL
CCHHHHHHHHCCCCCHHHHHHCCCCCHHHHHCCCCCCEEEECCCCCEECCCCCEEHHHHH
KSIQNSEEALAHLKTSKLDLFMNPPIAEINNLNKEGLISKKLSLESGNIIFNKKDYIDLN
HHHCCHHHHHHHHHHCCEEEEECCCHHHHHCCCCCCCEEHEEECCCCCEEECCCCEEEHH
LRRAIDSCISREDMCSKLLYGKSVPAIAYVPNNIAENDDISYGKNYFSLNKNLQRAKEFL
HHHHHHHHHCHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCHHHHCHHHHHHHHHH
DKSEYKKEKKDLKIISIEDPISNKIANSIIGELKENLDINLNLVNCKDENELEQKIKNGD
HHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHCCCC
YDLAFVRNKAKNNDPLFFLLNFTSDSLQYQDFLNKIKLEDNMWKKREYINKAQDVLAEDL
CEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YTIPVCFFYDMLCKRSRVQGQYITIDGNIIIKKITLAPYN
HHHHHHHHHHHHHHHHHCCCCEEEEECCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]