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The map label for this gene is fixJ [H]

Identifier: 170749678

GI number: 170749678

Start: 3473603

End: 3474010

Strand: Direct

Name: fixJ [H]

Synonym: Mrad2831_3276

Alternate gene names: 170749678

Gene position: 3473603-3474010 (Clockwise)

Preceding gene: 170749677

Following gene: 170749679

Centisome position: 57.15

GC content: 68.38

Gene sequence:

>408_bases
ATGCCGAAGCTCACGGGGCCGGTCCTCGTCGTCGACGACGACGAGGCCGTCCGCGACTCCCTGAAATTCGTCCTGGAGCT
CGAAGGATTGCAGGTCCGCCTGTACGCGGGCGCCGCGCAGCTTCTGCGCGACGTCGCCTTGCCGACGGACGGTTGTCTCC
TGGTCGATTACCGGATGCCCGACATGGACGGGCTCGAACTCGTGGCCCGGCTGCGCGCGCGCAACGTCGCTCTGCCGGCG
ATCCTGATCACCGGCCGCCTGTCGGGCGACCTGGGCCGCCTCGCGGCGGATGCCGGCTTCAGACGCGTGGTCGAGAAGCC
TTTCGAGGACGGCTCGCTCCTGGAAGGGATCCATGAGGCCCTGGCCCTCCACCCCGACGACTGGATCGGCCTCCGTAAAA
CCCCTTAA

Upstream 100 bases:

>100_bases
TTCGCGGGCCACGTGACACGCGGGGGACCTGCTTGAGGCAGATCAAGGCGCCCGGCCCGAGGCGGGCCTACGCCGGCCCG
ACGAAGGATCGGGCACCGCA

Downstream 100 bases:

>100_bases
GAGAAGCCCCCGAATGTCGCCGCGCCGGGGAAGGGCGTAGCCAAGAGCCACGTTCCGCTCCCGGAGGCTCCCATGCAGAC
CGCCCTCGCCGCCGCCGGCC

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 134

Protein sequence:

>135_residues
MPKLTGPVLVVDDDEAVRDSLKFVLELEGLQVRLYAGAAQLLRDVALPTDGCLLVDYRMPDMDGLELVARLRARNVALPA
ILITGRLSGDLGRLAADAGFRRVVEKPFEDGSLLEGIHEALALHPDDWIGLRKTP

Sequences:

>Translated_135_residues
MPKLTGPVLVVDDDEAVRDSLKFVLELEGLQVRLYAGAAQLLRDVALPTDGCLLVDYRMPDMDGLELVARLRARNVALPA
ILITGRLSGDLGRLAADAGFRRVVEKPFEDGSLLEGIHEALALHPDDWIGLRKTP
>Mature_134_residues
PKLTGPVLVVDDDEAVRDSLKFVLELEGLQVRLYAGAAQLLRDVALPTDGCLLVDYRMPDMDGLELVARLRARNVALPAI
LITGRLSGDLGRLAADAGFRRVVEKPFEDGSLLEGIHEALALHPDDWIGLRKTP

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=36.4406779661017, Blast_Score=61, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 14738; Mature: 14607

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLTGPVLVVDDDEAVRDSLKFVLELEGLQVRLYAGAAQLLRDVALPTDGCLLVDYRMP
CCCCCCCEEEECCCHHHHHHHHHHEEECCCEEEEECCHHHHHHHHCCCCCCEEEEEECCC
DMDGLELVARLRARNVALPAILITGRLSGDLGRLAADAGFRRVVEKPFEDGSLLEGIHEA
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LALHPDDWIGLRKTP
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
PKLTGPVLVVDDDEAVRDSLKFVLELEGLQVRLYAGAAQLLRDVALPTDGCLLVDYRMP
CCCCCCEEEECCCHHHHHHHHHHEEECCCEEEEECCHHHHHHHHCCCCCCEEEEEECCC
DMDGLELVARLRARNVALPAILITGRLSGDLGRLAADAGFRRVVEKPFEDGSLLEGIHEA
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LALHPDDWIGLRKTP
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]