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The map label for this gene is fixJ [H]

Identifier: 170749677

GI number: 170749677

Start: 3472794

End: 3473411

Strand: Direct

Name: fixJ [H]

Synonym: Mrad2831_3275

Alternate gene names: 170749677

Gene position: 3472794-3473411 (Clockwise)

Preceding gene: 170749676

Following gene: 170749678

Centisome position: 57.14

GC content: 71.68

Gene sequence:

>618_bases
ATGTCGAGTGACGCGCCCGTGCACATCGTGGACGACGATCTCGCGGTGCGCCAGGCGCTGGCCTTCCTGCTGGCGACCGA
CGGGCTCGCCGTCCGCGTCCACGACTCCGCGACCGCCTTCCTGGCCGCGGAGACCGAGCGCGTCGGGTGCGTCGTCACCG
ACGTGCGGATGCCCGGCATCGACGGCGTCGAGCTCCTGCACCGTCTACGCCAACGGGGCGGCCTGCCGCCGGTCATCGTC
ATGACGGGCCACGCGGACGTCGCCCTCGCGGTCGAGGCCATGAAGGCCGGCGCGGTCGACTTCATCGAGAAGCCGTTCGA
CGACGAGGTGCTCCTCGCCAGCATCCGCTCGGCCCTGGCGCGGGCGGACCGGACCAGCAAGCGCGCGTCCGAGCGCGACG
CGGTGCGGGCGCGCCTGGCCGAGCTGACCGAGCGCGAGCGGCAGGTGCTGGAAGGCTTGGTCGCCGGCAAGGCCAACAAG
GTCATCGGCCTCGACCTCGGCATCAGCCCGCGGACGGTGGAGATCTACCGCGCGAACGTCATGTCCAAACTTCAGGCGGG
CAGCCTCTCCGAGCTCGTCCGCATGGCTCTGATCGCGGGACAGGCGGACGGCGACTGA

Upstream 100 bases:

>100_bases
GTCGAGGCCCACGGCGGCAAGATCCAGGCCGGCCCCGGCCCGGACGGCGGGACCACCTTCCGCTTCACCCTGCGCACCCT
CGACCGCGAGGAAATGGCTC

Downstream 100 bases:

>100_bases
GCGCGGCGGCGACCCGGCATGTGTCCGGCCAGTCTTCCGGACCTCCGGGGCACCGTGTCGGGGCAGCGTCGGGCGCGAAG
CGGGAACCGCGTTCGCGGGC

Product: response regulator FixJ

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSSDAPVHIVDDDLAVRQALAFLLATDGLAVRVHDSATAFLAAETERVGCVVTDVRMPGIDGVELLHRLRQRGGLPPVIV
MTGHADVALAVEAMKAGAVDFIEKPFDDEVLLASIRSALARADRTSKRASERDAVRARLAELTERERQVLEGLVAGKANK
VIGLDLGISPRTVEIYRANVMSKLQAGSLSELVRMALIAGQADGD

Sequences:

>Translated_205_residues
MSSDAPVHIVDDDLAVRQALAFLLATDGLAVRVHDSATAFLAAETERVGCVVTDVRMPGIDGVELLHRLRQRGGLPPVIV
MTGHADVALAVEAMKAGAVDFIEKPFDDEVLLASIRSALARADRTSKRASERDAVRARLAELTERERQVLEGLVAGKANK
VIGLDLGISPRTVEIYRANVMSKLQAGSLSELVRMALIAGQADGD
>Mature_204_residues
SSDAPVHIVDDDLAVRQALAFLLATDGLAVRVHDSATAFLAAETERVGCVVTDVRMPGIDGVELLHRLRQRGGLPPVIVM
TGHADVALAVEAMKAGAVDFIEKPFDDEVLLASIRSALARADRTSKRASERDAVRARLAELTERERQVLEGLVAGKANKV
IGLDLGISPRTVEIYRANVMSKLQAGSLSELVRMALIAGQADGD

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=38.2608695652174, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790102, Length=196, Percent_Identity=36.2244897959184, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1788550, Length=120, Percent_Identity=35, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=36.8421052631579, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786747, Length=203, Percent_Identity=24.6305418719212, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788521, Length=192, Percent_Identity=30.7291666666667, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789809, Length=137, Percent_Identity=32.1167883211679, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1788712, Length=194, Percent_Identity=25.2577319587629, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=34.9593495934959, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI1788905, Length=215, Percent_Identity=29.3023255813954, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 21872; Mature: 21741

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDAPVHIVDDDLAVRQALAFLLATDGLAVRVHDSATAFLAAETERVGCVVTDVRMPGI
CCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCEEEEECCCCCC
DGVELLHRLRQRGGLPPVIVMTGHADVALAVEAMKAGAVDFIEKPFDDEVLLASIRSALA
CHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
RADRTSKRASERDAVRARLAELTERERQVLEGLVAGKANKVIGLDLGISPRTVEIYRANV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHHH
MSKLQAGSLSELVRMALIAGQADGD
HHHHCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSDAPVHIVDDDLAVRQALAFLLATDGLAVRVHDSATAFLAAETERVGCVVTDVRMPGI
CCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCEEEEECCCCCC
DGVELLHRLRQRGGLPPVIVMTGHADVALAVEAMKAGAVDFIEKPFDDEVLLASIRSALA
CHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHH
RADRTSKRASERDAVRARLAELTERERQVLEGLVAGKANKVIGLDLGISPRTVEIYRANV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEHHHHHHH
MSKLQAGSLSELVRMALIAGQADGD
HHHHCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]