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The map label for this gene is fixK [H]

Identifier: 170749679

GI number: 170749679

Start: 3474083

End: 3474763

Strand: Direct

Name: fixK [H]

Synonym: Mrad2831_3277

Alternate gene names: 170749679

Gene position: 3474083-3474763 (Clockwise)

Preceding gene: 170749678

Following gene: 170749683

Centisome position: 57.16

GC content: 72.39

Gene sequence:

>681_bases
ATGCAGACCGCCCTCGCCGCCGCCGGCCTCGCCCCCGTCTTCGGACGCGGCGCGCCGGCAGCCCTCCCCGCGTCGCTCGC
GTCCGACGCGCGGACCGTGACGCGCAGGACCCTCGCGGCCAACGAGGAGATCTACGCCGAGGGCGACCGGGCGATGTCCT
TCTACAAGATCGTCGTCGGGGTGGTGCGCACCTACAAGCTGCTGTCGGATGGTCGGCGGCAGATCGGCGCCTTCTACCTG
CCGGGCGACATCTTCGGTCTGGAATGCGGTGAGGAACACCGGTTCGGCGCCGACGCCGTCACCGAGACGCGGCTCGTCGT
CCATCGCCGCGAGCCGCGGGCGTTGACGGGCGACGACGGCAACCTCGCCCGCGAGGTCGTGGCCGCGATGATGCGCGACC
TGGAGCGGGCGCAGGAGCACATGATGCTGCTCGGCCGCCAATCCGCTCGGGAACGGATCGCGAGCTTCCTGCTCGCCCTG
TCGGAGCGGACGGCGGACGCGGGCGGGGCGGTCTGCCTGCCCATGTCGCGCAACGACATGGCCGACCATCTGGGCCTGAC
GATCGAGAGCGTCTCCCGCGCCGTCACCCAGCTCGAGCGGGCGGGGCTGATCGAGCTGCCTCCGAACCGCCGGACCGTCA
TCCTGCGCAACGCCGCCGCCCTGCGGCACCTCGCCGACTGA

Upstream 100 bases:

>100_bases
CTGGATCGGCCTCCGTAAAACCCCTTAAGAGAAGCCCCCGAATGTCGCCGCGCCGGGGAAGGGCGTAGCCAAGAGCCACG
TTCCGCTCCCGGAGGCTCCC

Downstream 100 bases:

>100_bases
CGGGCGCGCGGTCCGGTCGGATTGGGACGGTTCGGGAGGCAGGCCATCCCGGTCGATGCCACAGGCCCTCACGTAACCGC
GCGCCCGTCGCGGCAGAGGT

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MQTALAAAGLAPVFGRGAPAALPASLASDARTVTRRTLAANEEIYAEGDRAMSFYKIVVGVVRTYKLLSDGRRQIGAFYL
PGDIFGLECGEEHRFGADAVTETRLVVHRREPRALTGDDGNLAREVVAAMMRDLERAQEHMMLLGRQSARERIASFLLAL
SERTADAGGAVCLPMSRNDMADHLGLTIESVSRAVTQLERAGLIELPPNRRTVILRNAAALRHLAD

Sequences:

>Translated_226_residues
MQTALAAAGLAPVFGRGAPAALPASLASDARTVTRRTLAANEEIYAEGDRAMSFYKIVVGVVRTYKLLSDGRRQIGAFYL
PGDIFGLECGEEHRFGADAVTETRLVVHRREPRALTGDDGNLAREVVAAMMRDLERAQEHMMLLGRQSARERIASFLLAL
SERTADAGGAVCLPMSRNDMADHLGLTIESVSRAVTQLERAGLIELPPNRRTVILRNAAALRHLAD
>Mature_226_residues
MQTALAAAGLAPVFGRGAPAALPASLASDARTVTRRTLAANEEIYAEGDRAMSFYKIVVGVVRTYKLLSDGRRQIGAFYL
PGDIFGLECGEEHRFGADAVTETRLVVHRREPRALTGDDGNLAREVVAAMMRDLERAQEHMMLLGRQSARERIASFLLAL
SERTADAGGAVCLPMSRNDMADHLGLTIESVSRAVTQLERAGLIELPPNRRTVILRNAAALRHLAD

Specific function: FixK is a protein that regulates nitrogen fixation genes both positively and negatively. FixK is a positive regulator of nifA expression. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=197, Percent_Identity=28.4263959390863, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24521; Mature: 24521

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTALAAAGLAPVFGRGAPAALPASLASDARTVTRRTLAANEEIYAEGDRAMSFYKIVVG
CCCHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHH
VVRTYKLLSDGRRQIGAFYLPGDIFGLECGEEHRFGADAVTETRLVVHRREPRALTGDDG
HHHHHHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCCHHHHHHHEEECCCCCCCCCCCH
NLAREVVAAMMRDLERAQEHMMLLGRQSARERIASFLLALSERTADAGGAVCLPMSRNDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
ADHLGLTIESVSRAVTQLERAGLIELPPNRRTVILRNAAALRHLAD
HHHHCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECHHHHHHHCC
>Mature Secondary Structure
MQTALAAAGLAPVFGRGAPAALPASLASDARTVTRRTLAANEEIYAEGDRAMSFYKIVVG
CCCHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHH
VVRTYKLLSDGRRQIGAFYLPGDIFGLECGEEHRFGADAVTETRLVVHRREPRALTGDDG
HHHHHHHHHHHHHHHCEEECCCCEEECCCCCCCCCCCCHHHHHHHEEECCCCCCCCCCCH
NLAREVVAAMMRDLERAQEHMMLLGRQSARERIASFLLALSERTADAGGAVCLPMSRNDM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCH
ADHLGLTIESVSRAVTQLERAGLIELPPNRRTVILRNAAALRHLAD
HHHHCCHHHHHHHHHHHHHHCCCEECCCCCCEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1766374 [H]