Click here to switch to the map view.

The map label for this gene is appB [H]

Identifier: 170749623

GI number: 170749623

Start: 3406942

End: 3407913

Strand: Reverse

Name: appB [H]

Synonym: Mrad2831_3220

Alternate gene names: 170749623

Gene position: 3407913-3406942 (Counterclockwise)

Preceding gene: 170749624

Following gene: 170749622

Centisome position: 56.07

GC content: 69.34

Gene sequence:

>972_bases
ATGCCCCGCTTCATCGCCTCGCGCCTGGGCAAGATGGCGCTCATCCTGGTCGGGATCGTCATCCTCAACTTCGCGCTCAT
CCACGCGGCGCCGGGGGATCCGGCCTCGGTGATGGCGGGCGAGGCCGGCGATGCCGACGCCGCCTTCCTGCAGCAGTTGC
GCGAGCGCTTCGGCCTCGATCGGCCGCTCCCGATCCAGCTCCTGACCTACCTCGCGAACGTCCTGCGGCTCGACTTCGGC
TATTCCTACCGCCAGGGCATGCCGGTGATCGACCTGATCGCCGACCGGCTGCCCGCCACCCTGCTCCTGTCGGGCTCGGC
CTTCCTGATCTCCCTCGTCCTCGGCGTGGCGGCCGGGGCGCTGGCGGCCTCGCGCCAGGGCAAGGTCTCCGACTCCGTCA
TCTCGACCCTCGCCCTTCTCTTCTACGCCACGCCGCTGTTCTGGCTGGCGCTGATGGCGGTGCTGCTGTTCTCGGTGCAG
CTCGGGTGGTTTCCCGGCTTCGGCTACGAGAGCGTCGGCTCCGGCTATAGCGGCCTCGAACGGGCGGCCGATATCCTCCA
TCACCTCGTGCTGCCGGCCCTGACGCTCGGCCTGTTCTTCATGGCGGTCTACGCCCGCATGACCCGGGCCTCCGTGCTGG
AGGTGAGCCGGCTGGACTTCGTGCGGACCGCCCGCGCGAAGGGACTGCGCCCCCGCGTCGTGCTGGTCCGCCACATCCTG
CGCAACGCCATCCTCCCGGTCGTGACACTGGCGGGCCTCCAGGCGGGGCAACTCGTCGGCGGCGCCGTGCTGATCGAGAC
CGTCTTCGCGTGGCCGGGCATCGGCCGGCTGATGTTCGAGGCCCTCGAGCAGCGCGACTACAACCTCCTCCTCGGCATCT
TCGTCGTCTCCGCCGCGATGGTGCTGGCCTTCAACCTCATCACCGACATCCTCTACCGGCTGGTGGATCCCCGTATCGGA
GCTGCGGCATGA

Upstream 100 bases:

>100_bases
CACGACGCCTCATCCAGCGGCCCCGATCGTGGGGCCCCGTCCGACCCCGAGCCGCCGGCCGTTCCGGCGGGGCTCCCGCG
CGCGGAGGACCATACCCGTC

Downstream 100 bases:

>100_bases
CGGCGTTCTGGGCCCGATACGCCCGCAACCGCGGCGCGGTGGTGGGAATCGTCATCCTCGTCCTGGTGCTCGTCCTGGCG
ATCGCGGCGCCGGCGCTCTA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MPRFIASRLGKMALILVGIVILNFALIHAAPGDPASVMAGEAGDADAAFLQQLRERFGLDRPLPIQLLTYLANVLRLDFG
YSYRQGMPVIDLIADRLPATLLLSGSAFLISLVLGVAAGALAASRQGKVSDSVISTLALLFYATPLFWLALMAVLLFSVQ
LGWFPGFGYESVGSGYSGLERAADILHHLVLPALTLGLFFMAVYARMTRASVLEVSRLDFVRTARAKGLRPRVVLVRHIL
RNAILPVVTLAGLQAGQLVGGAVLIETVFAWPGIGRLMFEALEQRDYNLLLGIFVVSAAMVLAFNLITDILYRLVDPRIG
AAA

Sequences:

>Translated_323_residues
MPRFIASRLGKMALILVGIVILNFALIHAAPGDPASVMAGEAGDADAAFLQQLRERFGLDRPLPIQLLTYLANVLRLDFG
YSYRQGMPVIDLIADRLPATLLLSGSAFLISLVLGVAAGALAASRQGKVSDSVISTLALLFYATPLFWLALMAVLLFSVQ
LGWFPGFGYESVGSGYSGLERAADILHHLVLPALTLGLFFMAVYARMTRASVLEVSRLDFVRTARAKGLRPRVVLVRHIL
RNAILPVVTLAGLQAGQLVGGAVLIETVFAWPGIGRLMFEALEQRDYNLLLGIFVVSAAMVLAFNLITDILYRLVDPRIG
AAA
>Mature_322_residues
PRFIASRLGKMALILVGIVILNFALIHAAPGDPASVMAGEAGDADAAFLQQLRERFGLDRPLPIQLLTYLANVLRLDFGY
SYRQGMPVIDLIADRLPATLLLSGSAFLISLVLGVAAGALAASRQGKVSDSVISTLALLFYATPLFWLALMAVLLFSVQL
GWFPGFGYESVGSGYSGLERAADILHHLVLPALTLGLFFMAVYARMTRASVLEVSRLDFVRTARAKGLRPRVVLVRHILR
NAILPVVTLAGLQAGQLVGGAVLIETVFAWPGIGRLMFEALEQRDYNLLLGIFVVSAAMVLAFNLITDILYRLVDPRIGA
AA

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=336, Percent_Identity=40.4761904761905, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1787053, Length=319, Percent_Identity=38.2445141065831, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1789888, Length=320, Percent_Identity=35, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787497, Length=320, Percent_Identity=32.8125, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1787761, Length=329, Percent_Identity=35.258358662614, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1787550, Length=265, Percent_Identity=32.0754716981132, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1788504, Length=346, Percent_Identity=28.9017341040462, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34818; Mature: 34687

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRFIASRLGKMALILVGIVILNFALIHAAPGDPASVMAGEAGDADAAFLQQLRERFGLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCC
RPLPIQLLTYLANVLRLDFGYSYRQGMPVIDLIADRLPATLLLSGSAFLISLVLGVAAGA
CCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
LAASRQGKVSDSVISTLALLFYATPLFWLALMAVLLFSVQLGWFPGFGYESVGSGYSGLE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHH
RAADILHHLVLPALTLGLFFMAVYARMTRASVLEVSRLDFVRTARAKGLRPRVVLVRHIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RNAILPVVTLAGLQAGQLVGGAVLIETVFAWPGIGRLMFEALEQRDYNLLLGIFVVSAAM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLAFNLITDILYRLVDPRIGAAA
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PRFIASRLGKMALILVGIVILNFALIHAAPGDPASVMAGEAGDADAAFLQQLRERFGLD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCC
RPLPIQLLTYLANVLRLDFGYSYRQGMPVIDLIADRLPATLLLSGSAFLISLVLGVAAGA
CCCHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHH
LAASRQGKVSDSVISTLALLFYATPLFWLALMAVLLFSVQLGWFPGFGYESVGSGYSGLE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHH
RAADILHHLVLPALTLGLFFMAVYARMTRASVLEVSRLDFVRTARAKGLRPRVVLVRHIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RNAILPVVTLAGLQAGQLVGGAVLIETVFAWPGIGRLMFEALEQRDYNLLLGIFVVSAAM
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHH
VLAFNLITDILYRLVDPRIGAAA
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]