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The map label for this gene is gsiB [H]

Identifier: 170749624

GI number: 170749624

Start: 3408032

End: 3409585

Strand: Reverse

Name: gsiB [H]

Synonym: Mrad2831_3221

Alternate gene names: 170749624

Gene position: 3409585-3408032 (Counterclockwise)

Preceding gene: 170749626

Following gene: 170749623

Centisome position: 56.1

GC content: 63.45

Gene sequence:

>1554_bases
ATGTCGAACATTCTTCGAGCCTTGCTCACGATATCGGTTCTCGCCCTGCCATCTGTCGGACAGGCGCAGCCCGTCTCGGG
CGGTCGCGCCAACCTGATCGTTCAGCCGGAACCGCCCAGCCTGATGCTCGGTCTCGTGACCAACGCGCCGACGCAGCTCG
TAGCCGGGCAGATCTACGAAGGATTGCTGCGCTACGACGACAAGCTCAAGCCGCTGCCGTCCCTCGCGAAGAGCTGGGAT
ATCTCCCCTGACGGACTGCGTTACACGTTCCACCTCAATCAAGGCGTGACGTGGCACGATGGAAAGCCATTCAGCTCCGC
GGATGTCCTGTTCTCGGTCAACGAATTCCTGATGAAGACGCAGCCGCGTCACAGGAACACGATGACGCGTGTGGCCGATG
TCGGTGCGCCGGACGATCACACGGTGGTCTTCACCCTCAAGGAGCCCTTCGAGCCCTTCATCCGCAGCTTCGCCTTCTGG
GTAATGCCGATGGTGCCCAAACATATCTACGCGGGTACCGACTATGCCTCCAACCCGGCGAACGACAGGCCGATCGGCAC
CGGGCCGTTCAAGTTCGAGAGCTGGCGGAAGGGGAGCGCGATCCACCTCGTGAAGAACGAGGCTTACTATCTCGCGGGCA
AGCCGTATCTTCAGGACATCTACTATCACGTCATCCCGGACGGCGCCTCGCGCGCCGTTGCCTACGAGACCGGCAAGGTC
GACGTGCTGCCGGGCGGGTCGGTCGAGAATTTCGACGTGCCGCGGCTGACGGCCCTGAAGAACACCTGCGTCTCGCAGAA
GGGTCAGGAGTTCGACTCGCCCCTGTCGTTTCTCTGGCTCAACAACCGGAAGCCGCCGATGAACGACGTGCGGTTCCGCC
AGGCGGTCATGTACGCCATGGACCGGCAGTTCGCCCTCGACGTCCTGTGGAACGGGATCGGCAAGGTCGCCACCGGTCCG
ATCGCCGGGACGACGCCGTTCTACAAGTCCGTCCAGCCGCAGTATCCGCACGATCCGAAGAAGGCGAAGGCGCTGCTCGC
CGAGATGCACTACGACGGCAAGCCGCTGAAGCTGCTGCCGCTTCCCTATGGCGAGACGTGGCAGCGCTGGGCGGAGGCGG
TGAAGCAGAATCTCGGCGAGGTCGGCATCCCCGTCGAGATCCAGGCCACCGACGTGGCGGGCTGGAACCAGCGCGTGTCC
CAATGGGACTACGACATGGCCTTCACCTTCCTGTTCCAGAACGGCGACCCGGCCCTCGGCGTCGAGCGAAACTACATCAC
CTCGCAGATCTCCAAGGGCAATCCCTGGAACAACGTCGAGGGTTACTCGAACCCGACCGTGGACGCGCTGTTCGCTAAGG
CGGCGCTGGCGACGCCGGCTGAGAAGCGCCAGGATCTCTACGACGAGGTCCAGAAGGCGGTTCAGGCCGACGTGCCCGTG
GCGTGGCTGCTCGATCTGCGCTTCCCGGTCATCTACCGGTGCAACGTCCAGGACCTGATCACCACCGCCAACGGTCTCAA
CGACTCGCTGCGCGACACGTGGATCAAGCGCTGA

Upstream 100 bases:

>100_bases
GGCGGCGAAGAATAGTTCTCGAGTGCGACAAGATCAGAGCGAATAGGGTTTTCACGGGTTCGATTTTTCCGCGCATGTCG
TGGCAGTGTACGGAGCTTCC

Downstream 100 bases:

>100_bases
CGGCGCCGGACCGGCCCGCACGACGCCTCATCCAGCGGCCCCGATCGTGGGGCCCCGTCCGACCCCGAGCCGCCGGCCGT
TCCGGCGGGGCTCCCGCGCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MSNILRALLTISVLALPSVGQAQPVSGGRANLIVQPEPPSLMLGLVTNAPTQLVAGQIYEGLLRYDDKLKPLPSLAKSWD
ISPDGLRYTFHLNQGVTWHDGKPFSSADVLFSVNEFLMKTQPRHRNTMTRVADVGAPDDHTVVFTLKEPFEPFIRSFAFW
VMPMVPKHIYAGTDYASNPANDRPIGTGPFKFESWRKGSAIHLVKNEAYYLAGKPYLQDIYYHVIPDGASRAVAYETGKV
DVLPGGSVENFDVPRLTALKNTCVSQKGQEFDSPLSFLWLNNRKPPMNDVRFRQAVMYAMDRQFALDVLWNGIGKVATGP
IAGTTPFYKSVQPQYPHDPKKAKALLAEMHYDGKPLKLLPLPYGETWQRWAEAVKQNLGEVGIPVEIQATDVAGWNQRVS
QWDYDMAFTFLFQNGDPALGVERNYITSQISKGNPWNNVEGYSNPTVDALFAKAALATPAEKRQDLYDEVQKAVQADVPV
AWLLDLRFPVIYRCNVQDLITTANGLNDSLRDTWIKR

Sequences:

>Translated_517_residues
MSNILRALLTISVLALPSVGQAQPVSGGRANLIVQPEPPSLMLGLVTNAPTQLVAGQIYEGLLRYDDKLKPLPSLAKSWD
ISPDGLRYTFHLNQGVTWHDGKPFSSADVLFSVNEFLMKTQPRHRNTMTRVADVGAPDDHTVVFTLKEPFEPFIRSFAFW
VMPMVPKHIYAGTDYASNPANDRPIGTGPFKFESWRKGSAIHLVKNEAYYLAGKPYLQDIYYHVIPDGASRAVAYETGKV
DVLPGGSVENFDVPRLTALKNTCVSQKGQEFDSPLSFLWLNNRKPPMNDVRFRQAVMYAMDRQFALDVLWNGIGKVATGP
IAGTTPFYKSVQPQYPHDPKKAKALLAEMHYDGKPLKLLPLPYGETWQRWAEAVKQNLGEVGIPVEIQATDVAGWNQRVS
QWDYDMAFTFLFQNGDPALGVERNYITSQISKGNPWNNVEGYSNPTVDALFAKAALATPAEKRQDLYDEVQKAVQADVPV
AWLLDLRFPVIYRCNVQDLITTANGLNDSLRDTWIKR
>Mature_516_residues
SNILRALLTISVLALPSVGQAQPVSGGRANLIVQPEPPSLMLGLVTNAPTQLVAGQIYEGLLRYDDKLKPLPSLAKSWDI
SPDGLRYTFHLNQGVTWHDGKPFSSADVLFSVNEFLMKTQPRHRNTMTRVADVGAPDDHTVVFTLKEPFEPFIRSFAFWV
MPMVPKHIYAGTDYASNPANDRPIGTGPFKFESWRKGSAIHLVKNEAYYLAGKPYLQDIYYHVIPDGASRAVAYETGKVD
VLPGGSVENFDVPRLTALKNTCVSQKGQEFDSPLSFLWLNNRKPPMNDVRFRQAVMYAMDRQFALDVLWNGIGKVATGPI
AGTTPFYKSVQPQYPHDPKKAKALLAEMHYDGKPLKLLPLPYGETWQRWAEAVKQNLGEVGIPVEIQATDVAGWNQRVSQ
WDYDMAFTFLFQNGDPALGVERNYITSQISKGNPWNNVEGYSNPTVDALFAKAALATPAEKRQDLYDEVQKAVQADVPVA
WLLDLRFPVIYRCNVQDLITTANGLNDSLRDTWIKR

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787762, Length=431, Percent_Identity=29.4663573085847, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789966, Length=437, Percent_Identity=26.0869565217391, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1787052, Length=442, Percent_Identity=26.6968325791855, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1789887, Length=383, Percent_Identity=27.6762402088773, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787495, Length=550, Percent_Identity=24.1818181818182, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787551, Length=481, Percent_Identity=22.2453222453222, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1789397, Length=475, Percent_Identity=25.2631578947368, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081878, Length=500, Percent_Identity=23.6, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57832; Mature: 57700

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNILRALLTISVLALPSVGQAQPVSGGRANLIVQPEPPSLMLGLVTNAPTQLVAGQIYE
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHH
GLLRYDDKLKPLPSLAKSWDISPDGLRYTFHLNQGVTWHDGKPFSSADVLFSVNEFLMKT
HHHHHCCCCCCCHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHC
QPRHRNTMTRVADVGAPDDHTVVFTLKEPFEPFIRSFAFWVMPMVPKHIYAGTDYASNPA
CCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NDRPIGTGPFKFESWRKGSAIHLVKNEAYYLAGKPYLQDIYYHVIPDGASRAVAYETGKV
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHEEEECCCCCCCEEEEECCEE
DVLPGGSVENFDVPRLTALKNTCVSQKGQEFDSPLSFLWLNNRKPPMNDVRFRQAVMYAM
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
DRQFALDVLWNGIGKVATGPIAGTTPFYKSVQPQYPHDPKKAKALLAEMHYDGKPLKLLP
HHHHHHHHHHHCCCHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEE
LPYGETWQRWAEAVKQNLGEVGIPVEIQATDVAGWNQRVSQWDYDMAFTFLFQNGDPALG
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEECCCCCCC
VERNYITSQISKGNPWNNVEGYSNPTVDALFAKAALATPAEKRQDLYDEVQKAVQADVPV
CCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
AWLLDLRFPVIYRCNVQDLITTANGLNDSLRDTWIKR
EEEEECCCCEEEECCHHHHHHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
SNILRALLTISVLALPSVGQAQPVSGGRANLIVQPEPPSLMLGLVTNAPTQLVAGQIYE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCEEEEEECCCCHHHHHHHHHH
GLLRYDDKLKPLPSLAKSWDISPDGLRYTFHLNQGVTWHDGKPFSSADVLFSVNEFLMKT
HHHHHCCCCCCCHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHC
QPRHRNTMTRVADVGAPDDHTVVFTLKEPFEPFIRSFAFWVMPMVPKHIYAGTDYASNPA
CCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
NDRPIGTGPFKFESWRKGSAIHLVKNEAYYLAGKPYLQDIYYHVIPDGASRAVAYETGKV
CCCCCCCCCCCCCCCCCCCEEEEEECCEEEEECCHHHHHHEEEECCCCCCCEEEEECCEE
DVLPGGSVENFDVPRLTALKNTCVSQKGQEFDSPLSFLWLNNRKPPMNDVRFRQAVMYAM
EECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
DRQFALDVLWNGIGKVATGPIAGTTPFYKSVQPQYPHDPKKAKALLAEMHYDGKPLKLLP
HHHHHHHHHHHCCCHHCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEE
LPYGETWQRWAEAVKQNLGEVGIPVEIQATDVAGWNQRVSQWDYDMAFTFLFQNGDPALG
CCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEECCCCCCC
VERNYITSQISKGNPWNNVEGYSNPTVDALFAKAALATPAEKRQDLYDEVQKAVQADVPV
CCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCE
AWLLDLRFPVIYRCNVQDLITTANGLNDSLRDTWIKR
EEEEECCCCEEEECCHHHHHHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA