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The map label for this gene is narK [H]

Identifier: 170749004

GI number: 170749004

Start: 2752268

End: 2753650

Strand: Reverse

Name: narK [H]

Synonym: Mrad2831_2594

Alternate gene names: 170749004

Gene position: 2753650-2752268 (Counterclockwise)

Preceding gene: 170749006

Following gene: 170749003

Centisome position: 45.31

GC content: 68.55

Gene sequence:

>1383_bases
ATGCACGATCCCATCCCCGTCCCGAAGCGCGACCAGCGTCGCGCACTGTGGCTCTCGACCCTCGCCTTCACCACCTGCTT
TGCGGCCTGGACGATCTTCTCGATCATCGGCGTGCAGATAAAGAAGGACCTGGGACTCAGCGACGCCCAGTTCGGCCTCC
TGGTCGGGACACCGATCCTGACCGGCTCCCTGATACGCCTCATCCTCGGCATCTGGGCCGACCAGTATGGCGGGCGTCTG
GTCACACTCGCGACCATGCTGCTGGCGGCCCTCGCCACCTACCTCCTCACCTATGCCTACGACTACCCGACCTTCCTGCT
GGCCGCGCTCGGCGTCGGCCTCGCGGGCGGGGTCTTCTCTGTCGGCGTCACTTACGTGTCCAAGTGGTTCCCGAAGGAGA
GGCAGGGCACCGCCCTCGGCATCTTCGGGGCCGGCAACGTCGGCGCGGCGGTGACGAAGTTCCTGGCGCCGCTCGTCATG
GTGGCTCTCGGCTGGAAGGCGGTCGCCCAGGTCTGGTCGCTCGGCCTCGTCGTGATGGCACTGGTCTTCTATCTCGGGAC
CAAGGACGACCCGGACCTCGCCGCCCGCCGCCGTTCGGGTGCAAAGCCCGAGCCGCTCTCGGCGATGCTGGCGCCGCTCG
CCGACCTGCGGGTCTGGCGCTTCGCCCTGTACTACTTCTTCGTCTTCGGCGGGTTCGTCGCCCTCGCGCTCTGGCTGCCG
CGCTACTACACGGGCGTCTACGGGCTGGACATCGTCACTGCCGGCTGGCTCGCCGCCGCCTACTCGATCCCGGGCTCGCT
CTTCCGCATCCTCGGCGGCAGCCTGTCGGACCGCCACGGCGCCCGCAAGGTCATGTACTGGACCTTCCTGGGCTCCTGCG
CCTGCACCTTCCTGCTGGCGTACCCGGCCACCCACTACGTGGTCAGCGGCATCCGTGGGCCGATCTCGTTCGACCTCGCG
CTCGGGCCAATCGCCTTCACGGTGCTGACCTTCGCCCTCGGCTTCTTCATGAGCCTGGGCAAGGCGGCCGTGTTCAAGCA
CATCCCGGTCTACTACCCCGACCGGGTCGGGGCCGTGGGCGGCGTGGTCGGGTTAATCGGCGGCCTCGGCGGCTTCGTCC
TGCCCATCGCCTTCGGGTTCCTGAACGACCTGATCGGCGTCTGGACCTCGTGCTTCATGCTGCTCTTCGCCATCGTGGCG
GCGAGCACCCTCTGGATGCACGCCGCCGTGCGCGCCCAGGAGAAGCGGGCCCATCCCGAGCTCCGCCAGCCGAAGGACCT
GCCCGAGATCGAGGCCCTCAGGGCCGAGGTCGAGCGTCTGCGCGCGCGTCGGAACGACAACGTCGACGGTCTCCGGAATC
CCATCGGCGTCCCGGCCGAGTGA

Upstream 100 bases:

>100_bases
TCCCGAGCTCAACCCTGACTTTGCGCTGGATCAATTACGGCACGAGCCGACATCACGGACAGTGGTGGCACAAGAACGGC
CTGAACTGGATGCCGGTGCC

Downstream 100 bases:

>100_bases
ATGCCCCTGCCGGGGCACGGCCCCGGTCCCACTCCCCCACTGAAAGGCAACGTCCATGACCGCCGGCACGCAAACCCTCG
CGCCTGGCCGCCCCGCGGGC

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHDPIPVPKRDQRRALWLSTLAFTTCFAAWTIFSIIGVQIKKDLGLSDAQFGLLVGTPILTGSLIRLILGIWADQYGGRL
VTLATMLLAALATYLLTYAYDYPTFLLAALGVGLAGGVFSVGVTYVSKWFPKERQGTALGIFGAGNVGAAVTKFLAPLVM
VALGWKAVAQVWSLGLVVMALVFYLGTKDDPDLAARRRSGAKPEPLSAMLAPLADLRVWRFALYYFFVFGGFVALALWLP
RYYTGVYGLDIVTAGWLAAAYSIPGSLFRILGGSLSDRHGARKVMYWTFLGSCACTFLLAYPATHYVVSGIRGPISFDLA
LGPIAFTVLTFALGFFMSLGKAAVFKHIPVYYPDRVGAVGGVVGLIGGLGGFVLPIAFGFLNDLIGVWTSCFMLLFAIVA
ASTLWMHAAVRAQEKRAHPELRQPKDLPEIEALRAEVERLRARRNDNVDGLRNPIGVPAE

Sequences:

>Translated_460_residues
MHDPIPVPKRDQRRALWLSTLAFTTCFAAWTIFSIIGVQIKKDLGLSDAQFGLLVGTPILTGSLIRLILGIWADQYGGRL
VTLATMLLAALATYLLTYAYDYPTFLLAALGVGLAGGVFSVGVTYVSKWFPKERQGTALGIFGAGNVGAAVTKFLAPLVM
VALGWKAVAQVWSLGLVVMALVFYLGTKDDPDLAARRRSGAKPEPLSAMLAPLADLRVWRFALYYFFVFGGFVALALWLP
RYYTGVYGLDIVTAGWLAAAYSIPGSLFRILGGSLSDRHGARKVMYWTFLGSCACTFLLAYPATHYVVSGIRGPISFDLA
LGPIAFTVLTFALGFFMSLGKAAVFKHIPVYYPDRVGAVGGVVGLIGGLGGFVLPIAFGFLNDLIGVWTSCFMLLFAIVA
ASTLWMHAAVRAQEKRAHPELRQPKDLPEIEALRAEVERLRARRNDNVDGLRNPIGVPAE
>Mature_460_residues
MHDPIPVPKRDQRRALWLSTLAFTTCFAAWTIFSIIGVQIKKDLGLSDAQFGLLVGTPILTGSLIRLILGIWADQYGGRL
VTLATMLLAALATYLLTYAYDYPTFLLAALGVGLAGGVFSVGVTYVSKWFPKERQGTALGIFGAGNVGAAVTKFLAPLVM
VALGWKAVAQVWSLGLVVMALVFYLGTKDDPDLAARRRSGAKPEPLSAMLAPLADLRVWRFALYYFFVFGGFVALALWLP
RYYTGVYGLDIVTAGWLAAAYSIPGSLFRILGGSLSDRHGARKVMYWTFLGSCACTFLLAYPATHYVVSGIRGPISFDLA
LGPIAFTVLTFALGFFMSLGKAAVFKHIPVYYPDRVGAVGGVVGLIGGLGGFVLPIAFGFLNDLIGVWTSCFMLLFAIVA
ASTLWMHAAVRAQEKRAHPELRQPKDLPEIEALRAEVERLRARRNDNVDGLRNPIGVPAE

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=434, Percent_Identity=25.5760368663594, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=25.5458515283843, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367379, Length=298, Percent_Identity=23.489932885906, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49812; Mature: 49812

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51139 GTF2I ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCC
>Mature Secondary Structure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CCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]