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The map label for this gene is narK [H]

Identifier: 170749003

GI number: 170749003

Start: 2750845

End: 2752212

Strand: Reverse

Name: narK [H]

Synonym: Mrad2831_2593

Alternate gene names: 170749003

Gene position: 2752212-2750845 (Counterclockwise)

Preceding gene: 170749004

Following gene: 170749002

Centisome position: 45.28

GC content: 69.23

Gene sequence:

>1368_bases
ATGACCGCCGGCACGCAAACCCTCGCGCCTGGCCGCCCCGCGGGCGGCACCTGGCTCTCGCGCTGGGAACCCGAAAACCC
GGCCTTCTGGGCCGAGAGCGGCAGCCGCCTCGCCTGGCGCACCCTCGGCGTCACCACCGCCAACCTGACCATGTCGTTCA
TCGTGTGGTTCATGGTCTCCGCCTTGGTCGTCGCGCTCCCGGCGGTCGGGTTCAAGCTCACCACCGGACAGCTCTTCTGG
CTCGCCGCCATGCCGGGGCTCGCCGGCGGTACGCTGCGCCTCGTGCACATGTTCCTGACGCCGATGTTCGGCACCCGGCA
CGTCGTGACCCTGTCGAGCCTGTTGCTGCTGGTGCCGGCCATCGGCTGGTTCTACGCCGTGCAGGACCCGGCGACGCCCT
ACTGGGCGCTCCTCGTCCTGGCGTTCCTGGCCGGCCTCGGCGGTGGCAACTTCTCGTCCTTCATGCCCTCGACCAGCCTG
TTCTTTCCCAAGCGGCTGCAGGGCACGGCGCTCGCCGTCCAGGCCGGCGTCGGAAACTTCGGCGTGTCGGTCGTCCAGTT
CCTCACGCCCTGGGTCATCGGCTTCGCCCTCGCGGGCGGCACGCTGCTGGGAGAGTCGCAGACCATGACCAAGGGCGCCA
TCGAACGGCAGGTCTGGCTGCACAACGCCGCCGCGGTCTACATCCCCTTCATCGTGCTCTTCGCCCTGGCCGCGTGGGTC
GCGCTGCGCAGCGTGCCGGTGAAGGCCAACATGCGCGAGCAGATGGACATCTTCCGCTCGCGCCACACCTGGGCGATGAC
CTCGCTCTACATCATGACCTTCGGCGCGTTCTCCGGGCTTGCCGGCACGTTCCCGCTGCTGATCAAGCAGTGCTACGGGG
CCCTGCCGGGCGCCCCGGAGCCGCTGACCTACGCCTTCCTCGGACCGCTGGTCGGCTCGCTCCTGCGCGTGATGGCGGGG
CCGGTCTCGGACCGTCTCGGGGGCGCCAAGGTCACCCTGTTCGCAGGCATCGGCATGGCGGTCTGCGCCGCGCTCGTTCC
GCTCTACACCGCTCCGGCCTCGATGGAGAGCTTCCCGTACTTCGTCGGCGCCATGCTCGGCCTCTTTGCCTTCGCGGGCA
TCGGCAACGCGTCGACCTTCAAGCAGATGCCGATGATCTTCCCGCCCCGGCAGGCCGGCGGTGTCATCGGGTTCACCGGC
GCGGTCGCCGCCTACGGCCCGTTCGCCTTCGGCATGCTGTTCGCCTGGGCCTTCGGGACCTTCGGCGGCCCGAACGTCAT
CTTCTACGGTCTCGCACTGTTCTTCGCCCTCAACGTCGTGATGAACTGGTGGATGTACGCGCGCCGTGGGGCAGCGACCC
CCTGCTGA

Upstream 100 bases:

>100_bases
AACGTCGACGGTCTCCGGAATCCCATCGGCGTCCCGGCCGAGTGAATGCCCCTGCCGGGGCACGGCCCCGGTCCCACTCC
CCCACTGAAAGGCAACGTCC

Downstream 100 bases:

>100_bases
CAGGAAGGGGGCGGGCGGCACCTGACACACCATCAGAGCATATGCGGCCGGCGGAGGGGGCCCCTCCGCCGGCCGTTCGC
GTCAAGCGGGCCGAGCCGAC

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTAGTQTLAPGRPAGGTWLSRWEPENPAFWAESGSRLAWRTLGVTTANLTMSFIVWFMVSALVVALPAVGFKLTTGQLFW
LAAMPGLAGGTLRLVHMFLTPMFGTRHVVTLSSLLLLVPAIGWFYAVQDPATPYWALLVLAFLAGLGGGNFSSFMPSTSL
FFPKRLQGTALAVQAGVGNFGVSVVQFLTPWVIGFALAGGTLLGESQTMTKGAIERQVWLHNAAAVYIPFIVLFALAAWV
ALRSVPVKANMREQMDIFRSRHTWAMTSLYIMTFGAFSGLAGTFPLLIKQCYGALPGAPEPLTYAFLGPLVGSLLRVMAG
PVSDRLGGAKVTLFAGIGMAVCAALVPLYTAPASMESFPYFVGAMLGLFAFAGIGNASTFKQMPMIFPPRQAGGVIGFTG
AVAAYGPFAFGMLFAWAFGTFGGPNVIFYGLALFFALNVVMNWWMYARRGAATPC

Sequences:

>Translated_455_residues
MTAGTQTLAPGRPAGGTWLSRWEPENPAFWAESGSRLAWRTLGVTTANLTMSFIVWFMVSALVVALPAVGFKLTTGQLFW
LAAMPGLAGGTLRLVHMFLTPMFGTRHVVTLSSLLLLVPAIGWFYAVQDPATPYWALLVLAFLAGLGGGNFSSFMPSTSL
FFPKRLQGTALAVQAGVGNFGVSVVQFLTPWVIGFALAGGTLLGESQTMTKGAIERQVWLHNAAAVYIPFIVLFALAAWV
ALRSVPVKANMREQMDIFRSRHTWAMTSLYIMTFGAFSGLAGTFPLLIKQCYGALPGAPEPLTYAFLGPLVGSLLRVMAG
PVSDRLGGAKVTLFAGIGMAVCAALVPLYTAPASMESFPYFVGAMLGLFAFAGIGNASTFKQMPMIFPPRQAGGVIGFTG
AVAAYGPFAFGMLFAWAFGTFGGPNVIFYGLALFFALNVVMNWWMYARRGAATPC
>Mature_454_residues
TAGTQTLAPGRPAGGTWLSRWEPENPAFWAESGSRLAWRTLGVTTANLTMSFIVWFMVSALVVALPAVGFKLTTGQLFWL
AAMPGLAGGTLRLVHMFLTPMFGTRHVVTLSSLLLLVPAIGWFYAVQDPATPYWALLVLAFLAGLGGGNFSSFMPSTSLF
FPKRLQGTALAVQAGVGNFGVSVVQFLTPWVIGFALAGGTLLGESQTMTKGAIERQVWLHNAAAVYIPFIVLFALAAWVA
LRSVPVKANMREQMDIFRSRHTWAMTSLYIMTFGAFSGLAGTFPLLIKQCYGALPGAPEPLTYAFLGPLVGSLLRVMAGP
VSDRLGGAKVTLFAGIGMAVCAALVPLYTAPASMESFPYFVGAMLGLFAFAGIGNASTFKQMPMIFPPRQAGGVIGFTGA
VAAYGPFAFGMLFAWAFGTFGGPNVIFYGLALFFALNVVMNWWMYARRGAATPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=469, Percent_Identity=35.181236673774, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1787743, Length=458, Percent_Identity=33.4061135371179, Blast_Score=217, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48512; Mature: 48381

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGTQTLAPGRPAGGTWLSRWEPENPAFWAESGSRLAWRTLGVTTANLTMSFIVWFMVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHH
ALVVALPAVGFKLTTGQLFWLAAMPGLAGGTLRLVHMFLTPMFGTRHVVTLSSLLLLVPA
HHHHHHHHCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IGWFYAVQDPATPYWALLVLAFLAGLGGGNFSSFMPSTSLFFPKRLQGTALAVQAGVGNF
HHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHCCCCEEEEECCCCHH
GVSVVQFLTPWVIGFALAGGTLLGESQTMTKGAIERQVWLHNAAAVYIPFIVLFALAAWV
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALRSVPVKANMREQMDIFRSRHTWAMTSLYIMTFGAFSGLAGTFPLLIKQCYGALPGAPE
HHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLTYAFLGPLVGSLLRVMAGPVSDRLGGAKVTLFAGIGMAVCAALVPLYTAPASMESFPY
CHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHH
FVGAMLGLFAFAGIGNASTFKQMPMIFPPRQAGGVIGFTGAVAAYGPFAFGMLFAWAFGT
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
FGGPNVIFYGLALFFALNVVMNWWMYARRGAATPC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TAGTQTLAPGRPAGGTWLSRWEPENPAFWAESGSRLAWRTLGVTTANLTMSFIVWFMVS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCHHHHHHHHHHHHHHH
ALVVALPAVGFKLTTGQLFWLAAMPGLAGGTLRLVHMFLTPMFGTRHVVTLSSLLLLVPA
HHHHHHHHCCEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
IGWFYAVQDPATPYWALLVLAFLAGLGGGNFSSFMPSTSLFFPKRLQGTALAVQAGVGNF
HHHHEEECCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCHHCCCCEEEEECCCCHH
GVSVVQFLTPWVIGFALAGGTLLGESQTMTKGAIERQVWLHNAAAVYIPFIVLFALAAWV
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ALRSVPVKANMREQMDIFRSRHTWAMTSLYIMTFGAFSGLAGTFPLLIKQCYGALPGAPE
HHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PLTYAFLGPLVGSLLRVMAGPVSDRLGGAKVTLFAGIGMAVCAALVPLYTAPASMESFPY
CHHHHHHHHHHHHHHHHHHCCHHHCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCHHHHHH
FVGAMLGLFAFAGIGNASTFKQMPMIFPPRQAGGVIGFTGAVAAYGPFAFGMLFAWAFGT
HHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHC
FGGPNVIFYGLALFFALNVVMNWWMYARRGAATPC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]