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The map label for this gene is hmp [H]

Identifier: 170749006

GI number: 170749006

Start: 2754671

End: 2755915

Strand: Reverse

Name: hmp [H]

Synonym: Mrad2831_2596

Alternate gene names: 170749006

Gene position: 2755915-2754671 (Counterclockwise)

Preceding gene: 170749008

Following gene: 170749004

Centisome position: 45.34

GC content: 71.65

Gene sequence:

>1245_bases
ATGCCCGCCCCGCTATCGCCCGCCACCGTCGCGCTCATCAAGGCCACCGTGCCGGCGCTTGAGGCGCACGGCCTGACCAT
CACGAAGCGCATGTACGAGCGCCTGTTCCAGAACGCGGAGATCCGTGACCTCTTCAACCAGTCGCACCACGGCGAGACTG
GCTCGCAACCGAAGGCGCTCGCCCAGGCCGTGCTGGCCTACGCCCGCAACATCGACAACCTGGGCGTCCTCGGCAGCGCG
GTCGAGCGGATCGCGCAGAAGCATGTCGCGCTGAACATCCTACCCGAGCACTATCCCCACGTGGCCGACGCGCTGCTCGG
CGCCATCGGCGACGTGCTCGGCGCCGCGGCGACGCCCGAGATCTGCGCGGCCTGGGGCGAGGCGTACTGGTTCCTGGCCG
AGATCCTGATCGGCCGCGAGGCCGCGATCTACCGCGACCTCGCGGCAAAGCCCGGCGGCTGGAACGGTTGGCGCGACTTC
CACGTCGAGAACGTCGTGCAGGAGAGCGAGACCATCCGCTCCTTCGTGCTGGTGCCCGTCGACGGCGCCCCGGTGCTGCG
CCATGAGCCGGGCCAGTACCTGGGCTTCCTGTTCGACCTGCCCGGCCACGGCGTGCTCAAGCGCAACTACTCGATCTCGT
GCGCGCCGAACGACCGCGCCTACCGCATCACCGTCAAGCGCGAGGCCCATCCGGGCGCCCCCGCCGGCATCGTATCGAAT
TGGCTGCACGACCACGCCCGCTACGGCACGGTGCTGCGCGCGGCGCCCCCGGCCGGTGACTTCTTCCTCGACCGCGCGTC
CGAGGCGCCGGTGGTGCTGGTGAGCGGCGGCGTCGGCCTGACCCCCATGCTCAGCATGCTCGCGGCCATCGAGGCGGAGG
CCCCCGGCCGGCCGACGTGGTACGTGCACGGGGCCCTCAACGGCCGGGTGCACGCGATGCGCGGCGCCGCGTCCGCCGCG
GCCTCGGGCAACGCGAACATCCGCGTGCGGACGTTCTACGCCCAGCCCGAAGCACAGGACCGGCAGGGCGAGCACTACGA
CGAGGCTGGGCTGATCACGGCCGGCTGGCTCGTCGCGAACACGCCCCAGGCCGAGGCGACCTACTACCTGTGCGGGCCAA
AGCCGTTCCTGGCCTCGCTCGTGAACGGCCTGCGGCGGACCGGCGTCCCCGACGAGCGCGTGCGCTTCGAGTTCTTCGGG
CCTGCTGACGAGTTGGCCGAGGACGACGCCCGGCTCGCGGCGTGA

Upstream 100 bases:

>100_bases
CGCATGTCTAGTCCGTAACACCCCTTAAGGTATATACAGATTGCACCTTAAAACGAGCCTGGCCATAAGATGCATCGTCG
CTATTCCTTGAGGCCCGTCC

Downstream 100 bases:

>100_bases
CACAGCGGCCGGCGCCGCCCTCGACGGGGCGGTGCCGGCAACAGGGACGTCGGTCCCTTGGCGCGGTTCGCGTCCCTATC
CCCTCAGCCGCCCGATCAGC

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MPAPLSPATVALIKATVPALEAHGLTITKRMYERLFQNAEIRDLFNQSHHGETGSQPKALAQAVLAYARNIDNLGVLGSA
VERIAQKHVALNILPEHYPHVADALLGAIGDVLGAAATPEICAAWGEAYWFLAEILIGREAAIYRDLAAKPGGWNGWRDF
HVENVVQESETIRSFVLVPVDGAPVLRHEPGQYLGFLFDLPGHGVLKRNYSISCAPNDRAYRITVKREAHPGAPAGIVSN
WLHDHARYGTVLRAAPPAGDFFLDRASEAPVVLVSGGVGLTPMLSMLAAIEAEAPGRPTWYVHGALNGRVHAMRGAASAA
ASGNANIRVRTFYAQPEAQDRQGEHYDEAGLITAGWLVANTPQAEATYYLCGPKPFLASLVNGLRRTGVPDERVRFEFFG
PADELAEDDARLAA

Sequences:

>Translated_414_residues
MPAPLSPATVALIKATVPALEAHGLTITKRMYERLFQNAEIRDLFNQSHHGETGSQPKALAQAVLAYARNIDNLGVLGSA
VERIAQKHVALNILPEHYPHVADALLGAIGDVLGAAATPEICAAWGEAYWFLAEILIGREAAIYRDLAAKPGGWNGWRDF
HVENVVQESETIRSFVLVPVDGAPVLRHEPGQYLGFLFDLPGHGVLKRNYSISCAPNDRAYRITVKREAHPGAPAGIVSN
WLHDHARYGTVLRAAPPAGDFFLDRASEAPVVLVSGGVGLTPMLSMLAAIEAEAPGRPTWYVHGALNGRVHAMRGAASAA
ASGNANIRVRTFYAQPEAQDRQGEHYDEAGLITAGWLVANTPQAEATYYLCGPKPFLASLVNGLRRTGVPDERVRFEFFG
PADELAEDDARLAA
>Mature_413_residues
PAPLSPATVALIKATVPALEAHGLTITKRMYERLFQNAEIRDLFNQSHHGETGSQPKALAQAVLAYARNIDNLGVLGSAV
ERIAQKHVALNILPEHYPHVADALLGAIGDVLGAAATPEICAAWGEAYWFLAEILIGREAAIYRDLAAKPGGWNGWRDFH
VENVVQESETIRSFVLVPVDGAPVLRHEPGQYLGFLFDLPGHGVLKRNYSISCAPNDRAYRITVKREAHPGAPAGIVSNW
LHDHARYGTVLRAAPPAGDFFLDRASEAPVVLVSGGVGLTPMLSMLAAIEAEAPGRPTWYVHGALNGRVHAMRGAASAAA
SGNANIRVRTFYAQPEAQDRQGEHYDEAGLITAGWLVANTPQAEATYYLCGPKPFLASLVNGLRRTGVPDERVRFEFFGP
ADELAEDDARLAA

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=401, Percent_Identity=40.3990024937656, Blast_Score=276, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6321673, Length=414, Percent_Identity=35.2657004830918, Blast_Score=221, Evalue=2e-58,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR000951
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44620; Mature: 44489

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPLSPATVALIKATVPALEAHGLTITKRMYERLFQNAEIRDLFNQSHHGETGSQPKAL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
AQAVLAYARNIDNLGVLGSAVERIAQKHVALNILPEHYPHVADALLGAIGDVLGAAATPE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHCHHHHHHHHHHHHHHHHCCCCHH
ICAAWGEAYWFLAEILIGREAAIYRDLAAKPGGWNGWRDFHVENVVQESETIRSFVLVPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEC
DGAPVLRHEPGQYLGFLFDLPGHGVLKRNYSISCAPNDRAYRITVKREAHPGAPAGIVSN
CCCCCCCCCCCHHEEEEEECCCCCEEECCCEEEECCCCCEEEEEEEECCCCCCCHHHHHH
WLHDHARYGTVLRAAPPAGDFFLDRASEAPVVLVSGGVGLTPMLSMLAAIEAEAPGRPTW
HHHHHHHHCCEEEECCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
YVHGALNGRVHAMRGAASAAASGNANIRVRTFYAQPEAQDRQGEHYDEAGLITAGWLVAN
EEEECCCCEEEEECCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEECEEEEC
TPQAEATYYLCGPKPFLASLVNGLRRTGVPDERVRFEFFGPADELAEDDARLAA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHCCCHHCCC
>Mature Secondary Structure 
PAPLSPATVALIKATVPALEAHGLTITKRMYERLFQNAEIRDLFNQSHHGETGSQPKAL
CCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHH
AQAVLAYARNIDNLGVLGSAVERIAQKHVALNILPEHYPHVADALLGAIGDVLGAAATPE
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCHHCHHHHHHHHHHHHHHHHCCCCHH
ICAAWGEAYWFLAEILIGREAAIYRDLAAKPGGWNGWRDFHVENVVQESETIRSFVLVPV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEC
DGAPVLRHEPGQYLGFLFDLPGHGVLKRNYSISCAPNDRAYRITVKREAHPGAPAGIVSN
CCCCCCCCCCCHHEEEEEECCCCCEEECCCEEEECCCCCEEEEEEEECCCCCCCHHHHHH
WLHDHARYGTVLRAAPPAGDFFLDRASEAPVVLVSGGVGLTPMLSMLAAIEAEAPGRPTW
HHHHHHHHCCEEEECCCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
YVHGALNGRVHAMRGAASAAASGNANIRVRTFYAQPEAQDRQGEHYDEAGLITAGWLVAN
EEEECCCCEEEEECCCHHHCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEECEEEEC
TPQAEATYYLCGPKPFLASLVNGLRRTGVPDERVRFEFFGPADELAEDDARLAA
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCHHEEEEEECCHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]