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The map label for this gene is murE [H]

Identifier: 170748780

GI number: 170748780

Start: 2500963

End: 2502420

Strand: Reverse

Name: murE [H]

Synonym: Mrad2831_2362

Alternate gene names: 170748780

Gene position: 2502420-2500963 (Counterclockwise)

Preceding gene: 170748781

Following gene: 170748779

Centisome position: 41.17

GC content: 76.95

Gene sequence:

>1458_bases
ATGAGCGCGCGGCTCGCCGACCTCTTCCCCGAGGCCGCCGGCCCCGCCGCGGACCTGACCGTCTCGGACCTCACGGCCGA
CAGCCGGCAGGTCGCGACCGGCACGGTGTTCGTCGCCGTGCCGGGGACGAAGGCCGACGGCCGCCGCTTCGCCGCGCAGG
CCGCCCAGCGCGGCGCCGCCGCGGTGGCCGGTGAGGGGACCCGTCCCGCCGACCTGCCGGACGCGGTCGCCTGGATCCCC
GTCGCCGACGCGCGCCGGGCGCTCGCCCTGGCCGCGGCCCGTCTCGCGGGTCCGCAGCCCGAGACCGTCGTGGCGGTGAC
CGGCACCGCCGGGAAGAGCTCGGTGGCGGACTTCGTCCGCCAGATCCTGGCGCGCCTCGGCCGCGACGCCGCGAGCCTCG
GCACCGTCGGTATCGTCACCGGTCGCGGCGCGCAGTACGGCTCCCTCACCACCCCGGACCCGGTGACGCTGCACCGGACG
CTGGCGCGGCTCGCCGCGGAGGGCGTCACCGACCTCGCCATGGAGGCGTCCTCCCACGGGATCGAGCAGCGCCGGCTCGA
CGGCGTGCGGCTGGACGCGGCCGGCTTCACCAATCTCGGCCGCGATCATCTCGACTATCACGCGAGCATCGAGGAGTACC
TCGCGGCCAAGCTGCGCCTGTTCACCGACCTGCTGCCGCCGGGACGCCCCGCCATCGTCAACGCCGACGGCGCCTATGCC
GAGCGGGTGATCGCCGCGGCCGAGGCCGCCGGCCGGCCGGTCCGCACGGTCGGGCGCGGCGGCCGGGACCTGCGCGTCGA
GGCGGTCCGCACGGAGGGGTTCGCGCAGGCCGCCCGCGTCCTGTTCGCGGGCCGGGCCCGGGACCTGCGCCTGCCGCTCG
TCGGCGAGTTCCAGGTCGAGAACGCCCTGGTGGCGGCCGGCCTCGCGCTGGCCACCCCGGCGGGGGCGGCCGATCCCGAC
GGGGTCTTCGCGGCCCTCGACCACCTCACGGGCGTCCCCGGCCGCATGGAGCGGATCGGCGAGGCGCGGGGCGGACTCTG
TCTCGTCGACTACGCGCACAAGCCCGAGGCCCTGGAGAGCGTCCTGACCGCCCTGCGGCCCTTCGCGACCGGCCGGCTGG
TCGCGGTGTTCGGCTGCGGCGGCGACCGCGACCGGGGCAAGCGGCCCCTGATGGGCGCCATCGCGCAGCGGCTCGCCGAC
GTCGTGATCGTCACGGACGACAACCCGCGCACAGAGGACGCGGCCGCCATCCGCCGGGCCATCCTGGACGCGGCGCCGGG
CGCGGAGGAGATCGGCGACCGGTCGGAGGCGATTCACAGCGCCGTCAGCCGGCTCGGACCGGGCGACGTGCTGGTCGTGG
CCGGCAAGGGTCATGAAACCGGCCAGATCGTGGGAGATCGCACGCTGCCGTTCTCGGACCACGCGGTCCTGCGCGCCGCG
ATCGCCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
CGAGCCGCCGGTGAGGCCGTTCCCGCAGATGGTCAAGCTCAATGCCTACCACGTCAACCAGCTCGGCGGCCCGTGAGATT
GGATCGATGAGGGTGGATCG

Downstream 100 bases:

>100_bases
GGACGACGGGGGACCGACGATGACCGCACTCTGGACGCGGGAGGAGCTGGAATCCGCGACGGGCGGCTGCCTGACCGGCG
CCGAGCGCGCCATCTCGGGC

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSARLADLFPEAAGPAADLTVSDLTADSRQVATGTVFVAVPGTKADGRRFAAQAAQRGAAAVAGEGTRPADLPDAVAWIP
VADARRALALAAARLAGPQPETVVAVTGTAGKSSVADFVRQILARLGRDAASLGTVGIVTGRGAQYGSLTTPDPVTLHRT
LARLAAEGVTDLAMEASSHGIEQRRLDGVRLDAAGFTNLGRDHLDYHASIEEYLAAKLRLFTDLLPPGRPAIVNADGAYA
ERVIAAAEAAGRPVRTVGRGGRDLRVEAVRTEGFAQAARVLFAGRARDLRLPLVGEFQVENALVAAGLALATPAGAADPD
GVFAALDHLTGVPGRMERIGEARGGLCLVDYAHKPEALESVLTALRPFATGRLVAVFGCGGDRDRGKRPLMGAIAQRLAD
VVIVTDDNPRTEDAAAIRRAILDAAPGAEEIGDRSEAIHSAVSRLGPGDVLVVAGKGHETGQIVGDRTLPFSDHAVLRAA
IAAAA

Sequences:

>Translated_485_residues
MSARLADLFPEAAGPAADLTVSDLTADSRQVATGTVFVAVPGTKADGRRFAAQAAQRGAAAVAGEGTRPADLPDAVAWIP
VADARRALALAAARLAGPQPETVVAVTGTAGKSSVADFVRQILARLGRDAASLGTVGIVTGRGAQYGSLTTPDPVTLHRT
LARLAAEGVTDLAMEASSHGIEQRRLDGVRLDAAGFTNLGRDHLDYHASIEEYLAAKLRLFTDLLPPGRPAIVNADGAYA
ERVIAAAEAAGRPVRTVGRGGRDLRVEAVRTEGFAQAARVLFAGRARDLRLPLVGEFQVENALVAAGLALATPAGAADPD
GVFAALDHLTGVPGRMERIGEARGGLCLVDYAHKPEALESVLTALRPFATGRLVAVFGCGGDRDRGKRPLMGAIAQRLAD
VVIVTDDNPRTEDAAAIRRAILDAAPGAEEIGDRSEAIHSAVSRLGPGDVLVVAGKGHETGQIVGDRTLPFSDHAVLRAA
IAAAA
>Mature_484_residues
SARLADLFPEAAGPAADLTVSDLTADSRQVATGTVFVAVPGTKADGRRFAAQAAQRGAAAVAGEGTRPADLPDAVAWIPV
ADARRALALAAARLAGPQPETVVAVTGTAGKSSVADFVRQILARLGRDAASLGTVGIVTGRGAQYGSLTTPDPVTLHRTL
ARLAAEGVTDLAMEASSHGIEQRRLDGVRLDAAGFTNLGRDHLDYHASIEEYLAAKLRLFTDLLPPGRPAIVNADGAYAE
RVIAAAEAAGRPVRTVGRGGRDLRVEAVRTEGFAQAARVLFAGRARDLRLPLVGEFQVENALVAAGLALATPAGAADPDG
VFAALDHLTGVPGRMERIGEARGGLCLVDYAHKPEALESVLTALRPFATGRLVAVFGCGGDRDRGKRPLMGAIAQRLADV
VIVTDDNPRTEDAAAIRRAILDAAPGAEEIGDRSEAIHSAVSRLGPGDVLVVAGKGHETGQIVGDRTLPFSDHAVLRAAI
AAAA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=484, Percent_Identity=38.0165289256198, Blast_Score=227, Evalue=2e-60,
Organism=Escherichia coli, GI1786274, Length=345, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 49960; Mature: 49829

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARLADLFPEAAGPAADLTVSDLTADSRQVATGTVFVAVPGTKADGRRFAAQAAQRGAA
CCCCHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHCCCE
AVAGEGTRPADLPDAVAWIPVADARRALALAAARLAGPQPETVVAVTGTAGKSSVADFVR
EECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
QILARLGRDAASLGTVGIVTGRGAQYGSLTTPDPVTLHRTLARLAAEGVTDLAMEASSHG
HHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCC
IEQRRLDGVRLDAAGFTNLGRDHLDYHASIEEYLAAKLRLFTDLLPPGRPAIVNADGAYA
CHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
ERVIAAAEAAGRPVRTVGRGGRDLRVEAVRTEGFAQAARVLFAGRARDLRLPLVGEFQVE
HHHHHHHHHCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCEECCCCCCHHHH
NALVAAGLALATPAGAADPDGVFAALDHLTGVPGRMERIGEARGGLCLVDYAHKPEALES
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCHHHHH
VLTALRPFATGRLVAVFGCGGDRDRGKRPLMGAIAQRLADVVIVTDDNPRTEDAAAIRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
ILDAAPGAEEIGDRSEAIHSAVSRLGPGDVLVVAGKGHETGQIVGDRTLPFSDHAVLRAA
HHHCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCHHHHHHHH
IAAAA
HHHCC
>Mature Secondary Structure 
SARLADLFPEAAGPAADLTVSDLTADSRQVATGTVFVAVPGTKADGRRFAAQAAQRGAA
CCCHHHHCCCCCCCCCCCCHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHCCCE
AVAGEGTRPADLPDAVAWIPVADARRALALAAARLAGPQPETVVAVTGTAGKSSVADFVR
EECCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
QILARLGRDAASLGTVGIVTGRGAQYGSLTTPDPVTLHRTLARLAAEGVTDLAMEASSHG
HHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCC
IEQRRLDGVRLDAAGFTNLGRDHLDYHASIEEYLAAKLRLFTDLLPPGRPAIVNADGAYA
CHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHH
ERVIAAAEAAGRPVRTVGRGGRDLRVEAVRTEGFAQAARVLFAGRARDLRLPLVGEFQVE
HHHHHHHHHCCCCHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCEECCCCCCHHHH
NALVAAGLALATPAGAADPDGVFAALDHLTGVPGRMERIGEARGGLCLVDYAHKPEALES
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCHHHHH
VLTALRPFATGRLVAVFGCGGDRDRGKRPLMGAIAQRLADVVIVTDDNPRTEDAAAIRRA
HHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
ILDAAPGAEEIGDRSEAIHSAVSRLGPGDVLVVAGKGHETGQIVGDRTLPFSDHAVLRAA
HHHCCCCHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCHHHHHHHH
IAAAA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]