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The map label for this gene is murF [H]

Identifier: 170748779

GI number: 170748779

Start: 2499495

End: 2500943

Strand: Reverse

Name: murF [H]

Synonym: Mrad2831_2361

Alternate gene names: 170748779

Gene position: 2500943-2499495 (Counterclockwise)

Preceding gene: 170748780

Following gene: 170748778

Centisome position: 41.15

GC content: 75.5

Gene sequence:

>1449_bases
ATGACCGCACTCTGGACGCGGGAGGAGCTGGAATCCGCGACGGGCGGCTGCCTGACCGGCGCCGAGCGCGCCATCTCGGG
CGCCTCGATCGACACCCGCACGCTCCAGCCCGGCGACCTGTTCTTCGCGATCCGCGGCGATGCCCGCGACGGCCACGACT
TCGTCCCCGACGCCCTGGGGCGCGGGGCCGGTGCCGCGGTGGTGAGCGCCGCGCGCGCCGAGGCGCTCGCCGCCCACGGC
CCCGTCCTGGCGGTGCCCGAGGGGGGCGCCGACCCGGTCCTGACCGCCATGGTGCGGCTCGGCGCCGCCGCCCGGGCCCG
GACGTCCGCGCAGGTCGTGGCGGTCACCGGCTCGGTCGGCAAGACCGGGACCAAGGAAGCGTTGCGCCACGTCCTGTCCG
AGCAGGGCGAGACTCACGCCTCGGCGGCCTCCTACAACAACCACTGGGGCGTGCCCCTGACCCTGACGCGGATGCCGCGC
TCCGCGCGCTACGGCGTGTTCGAGATCGGCATGAACCACGGCGCCGAGATCGTGCCGCTGACCGCCCAGGTCCGGCCGGA
CATCGCGCTGATCACCACGATCGCGCCCGCCCATATCGAGCACTTCGCCTCGCTCGCCGCGATCGCGGACGCCAAGGGCG
AGATCTTCTCCGGCCTCCGGCCCGGCGGGGTGGCGATCCTGAACCGCGACGCCCCGCATTTCGACCGGCTGGCCGCCCAC
GCCCAGGCCTCCCGGGCGGGCCGGGTGGTGACCTTCGGCGAGCACCCGGATGCCGATGTCCGCGCCCTGAAGATCGTGCT
CCGGCCCGACCTGTCGGTGATCGACGCGGCGGTGATGGGCCAGCCCGTCACCTACAAGCTCGGCACGGCCGGCCGGCACG
CGGCGCTGAACTCGCTGGGCGTCATGGCGGTGGTCCACAGCCTCGGGGCCGACCTCGCCCGCGCGGCCCTGTCGCTCGGC
GGGCTGACGCCTCCGGCGGGGCGCGGCGAGCGCACCGCCCTCGAGATCGGCGGCGGCACCGCCTACCTCGTGGACGAGAG
CTACAACGCCAATCCCGTCGCGGTCCGCGCGGCGCTGGCGACCCTGGCGGGGATCGAGACCGGGCCCCGCGGCCGCCGGA
TCGCGGTGCTGGGCGACATGCTGGAGCTCGGCGCCGCCGCGCCCGACCTGCATCGCGGCCTGGCGGAGGCGATCGAGGCG
GCCCGGGTCGACCTGGTCTTCACGGCCGGTCCGCTCATGCGCAACCTGTTCGAGGCCCTTCCGGTGAGCCGCCGCGGCGC
GGTGGCCGACACCGCGGCCGACCTGCTCGAGCCGCTCGCCCGGACCCTGCGGTCCGGCGACGCCGTCATGGTGAAGGGAT
CGAACGGCAGCCGCATGGGCCGGATTGTCGAGGCGCTGAAAGCCCGCTACGCGGTCGATCCGGCCCGGCGGACCCAGGCC
GCCCCGTGA

Upstream 100 bases:

>100_bases
ACCGGCCAGATCGTGGGAGATCGCACGCTGCCGTTCTCGGACCACGCGGTCCTGCGCGCCGCGATCGCCGCGGCGGCCTG
AGGACGACGGGGGACCGACG

Downstream 100 bases:

>100_bases
CCGGCACGCCTGCGCGTTCGGCGCCGTCCCCCGAAACCTGAGCCTGAACAAGGGGGCCGAGCGCCCGGATGCTGTACCTT
CTCTCGGACCTGAGCAGCAG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTALWTREELESATGGCLTGAERAISGASIDTRTLQPGDLFFAIRGDARDGHDFVPDALGRGAGAAVVSAARAEALAAHG
PVLAVPEGGADPVLTAMVRLGAAARARTSAQVVAVTGSVGKTGTKEALRHVLSEQGETHASAASYNNHWGVPLTLTRMPR
SARYGVFEIGMNHGAEIVPLTAQVRPDIALITTIAPAHIEHFASLAAIADAKGEIFSGLRPGGVAILNRDAPHFDRLAAH
AQASRAGRVVTFGEHPDADVRALKIVLRPDLSVIDAAVMGQPVTYKLGTAGRHAALNSLGVMAVVHSLGADLARAALSLG
GLTPPAGRGERTALEIGGGTAYLVDESYNANPVAVRAALATLAGIETGPRGRRIAVLGDMLELGAAAPDLHRGLAEAIEA
ARVDLVFTAGPLMRNLFEALPVSRRGAVADTAADLLEPLARTLRSGDAVMVKGSNGSRMGRIVEALKARYAVDPARRTQA
AP

Sequences:

>Translated_482_residues
MTALWTREELESATGGCLTGAERAISGASIDTRTLQPGDLFFAIRGDARDGHDFVPDALGRGAGAAVVSAARAEALAAHG
PVLAVPEGGADPVLTAMVRLGAAARARTSAQVVAVTGSVGKTGTKEALRHVLSEQGETHASAASYNNHWGVPLTLTRMPR
SARYGVFEIGMNHGAEIVPLTAQVRPDIALITTIAPAHIEHFASLAAIADAKGEIFSGLRPGGVAILNRDAPHFDRLAAH
AQASRAGRVVTFGEHPDADVRALKIVLRPDLSVIDAAVMGQPVTYKLGTAGRHAALNSLGVMAVVHSLGADLARAALSLG
GLTPPAGRGERTALEIGGGTAYLVDESYNANPVAVRAALATLAGIETGPRGRRIAVLGDMLELGAAAPDLHRGLAEAIEA
ARVDLVFTAGPLMRNLFEALPVSRRGAVADTAADLLEPLARTLRSGDAVMVKGSNGSRMGRIVEALKARYAVDPARRTQA
AP
>Mature_481_residues
TALWTREELESATGGCLTGAERAISGASIDTRTLQPGDLFFAIRGDARDGHDFVPDALGRGAGAAVVSAARAEALAAHGP
VLAVPEGGADPVLTAMVRLGAAARARTSAQVVAVTGSVGKTGTKEALRHVLSEQGETHASAASYNNHWGVPLTLTRMPRS
ARYGVFEIGMNHGAEIVPLTAQVRPDIALITTIAPAHIEHFASLAAIADAKGEIFSGLRPGGVAILNRDAPHFDRLAAHA
QASRAGRVVTFGEHPDADVRALKIVLRPDLSVIDAAVMGQPVTYKLGTAGRHAALNSLGVMAVVHSLGADLARAALSLGG
LTPPAGRGERTALEIGGGTAYLVDESYNANPVAVRAALATLAGIETGPRGRRIAVLGDMLELGAAAPDLHRGLAEAIEAA
RVDLVFTAGPLMRNLFEALPVSRRGAVADTAADLLEPLARTLRSGDAVMVKGSNGSRMGRIVEALKARYAVDPARRTQAA
P

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=468, Percent_Identity=33.974358974359, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 49690; Mature: 49559

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALWTREELESATGGCLTGAERAISGASIDTRTLQPGDLFFAIRGDARDGHDFVPDALG
CCCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHC
RGAGAAVVSAARAEALAAHGPVLAVPEGGADPVLTAMVRLGAAARARTSAQVVAVTGSVG
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KTGTKEALRHVLSEQGETHASAASYNNHWGVPLTLTRMPRSARYGVFEIGMNHGAEIVPL
CCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCEEEEE
TAQVRPDIALITTIAPAHIEHFASLAAIADAKGEIFSGLRPGGVAILNRDAPHFDRLAAH
EECCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCHHHHHHHH
AQASRAGRVVTFGEHPDADVRALKIVLRPDLSVIDAAVMGQPVTYKLGTAGRHAALNSLG
HHHHCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
VMAVVHSLGADLARAALSLGGLTPPAGRGERTALEIGGGTAYLVDESYNANPVAVRAALA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
TLAGIETGPRGRRIAVLGDMLELGAAAPDLHRGLAEAIEAARVDLVFTAGPLMRNLFEAL
HHHCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHC
PVSRRGAVADTAADLLEPLARTLRSGDAVMVKGSNGSRMGRIVEALKARYAVDPARRTQA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCC
AP
CC
>Mature Secondary Structure 
TALWTREELESATGGCLTGAERAISGASIDTRTLQPGDLFFAIRGDARDGHDFVPDALG
CCCCCHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHC
RGAGAAVVSAARAEALAAHGPVLAVPEGGADPVLTAMVRLGAAARARTSAQVVAVTGSVG
CCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCC
KTGTKEALRHVLSEQGETHASAASYNNHWGVPLTLTRMPRSARYGVFEIGMNHGAEIVPL
CCCHHHHHHHHHHHCCCCHHHHCCCCCCCCCCEEEECCCCCCCCCEEEECCCCCCEEEEE
TAQVRPDIALITTIAPAHIEHFASLAAIADAKGEIFSGLRPGGVAILNRDAPHFDRLAAH
EECCCCCEEEEEEECHHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEECCCCCHHHHHHHH
AQASRAGRVVTFGEHPDADVRALKIVLRPDLSVIDAAVMGQPVTYKLGTAGRHAALNSLG
HHHHCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCHHHHHHHH
VMAVVHSLGADLARAALSLGGLTPPAGRGERTALEIGGGTAYLVDESYNANPVAVRAALA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHH
TLAGIETGPRGRRIAVLGDMLELGAAAPDLHRGLAEAIEAARVDLVFTAGPLMRNLFEAL
HHHCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHC
PVSRRGAVADTAADLLEPLARTLRSGDAVMVKGSNGSRMGRIVEALKARYAVDPARRTQA
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHHCCC
AP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]