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The map label for this gene is thiD [H]

Identifier: 170748376

GI number: 170748376

Start: 2088714

End: 2089529

Strand: Reverse

Name: thiD [H]

Synonym: Mrad2831_1958

Alternate gene names: 170748376

Gene position: 2089529-2088714 (Counterclockwise)

Preceding gene: 170748377

Following gene: 170748375

Centisome position: 34.38

GC content: 75.37

Gene sequence:

>816_bases
ATGAGCGGCCCGGCGCCCATCGCGGTCACGATCGCGGGCTCGGATTCCGGCGGCGGTGCCGGCATCCAGGCGGACCTCAA
GACCTTCTCGGCGCTGGGCGTCTACGGCGCCAGCGTGATCACGGCCCTGACCGCGCAGAACACGATCGGCGTCCAGGGCA
TCCACGACGTGCCGGCGGATTTCGTCGCCCGGCAGATCGACAGCGTGTTCGCCGACCTCGCCGTGAAGGCCGTGAAGATC
GGCATGCTGTCGCAGACCGCGGTGATCGCGGCGGTCGCCGAAGGCCTGGCGCGGCGCGCCGGAGCGATCCCGGTGGTCCT
CGATCCGGTGATGGTCGCCACCAGCGGCGACCGGCTGATCACCGACGCCGCCGTGGCGACGCTGCGCGAGCGGCTGCTGC
CCCGGGCCGACCTGATCACCCCCAACCTGCCGGAGGCGGCGGTGCTCCTCGGCGAGCCCGTCGCCGCGGACGCGGCGGCG
ATCGTGGCCCAGGCGCGCCGGCTGGTCGCCCTCGGCGCCAGGGCGGTGCTGATCAAGGGCGGCCACGGCACGGGGCGGGA
GAGCATCGACCACCTCGTGACGGCGGACGGCGCCCTGCGGACCCTGTCGGCGCCGCGGATCGCGACGCGCAACACCCACG
GCACCGGCTGCACGCTCTCGGCCGCGGTCGCGGCGGGCCTCGCCCAGGGCCTGGCGCTGCCGGAGGCGGTGGCGGAGGCC
AAGGCCTACCTCACGGCCGCCCTCGCGGCGGCGGACAGCCTCGCGGTCGGAACGGGGCACGGGCCGGTCCACCACTTCCA
CGCCGTCTGGTCCTAG

Upstream 100 bases:

>100_bases
CGGTCATCACCGCCCTGTTCGGCGCCGACTCGGTGCGCCAGCGCGCCCACGACCTGCGCGCGCGGATCGACGGCGCCCTG
GCGGAGCGGGCCGGCGCCCG

Downstream 100 bases:

>100_bases
GCCGGTCCTGGCCCGGTCCTGGGCCGGCGCTTTCCGGTGCGCACGCCACTGCGCGCGCCGGGCGCCGCCTTGCAACCGGG
CGTCGATTGCGTTTGAAGAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSGPAPIAVTIAGSDSGGGAGIQADLKTFSALGVYGASVITALTAQNTIGVQGIHDVPADFVARQIDSVFADLAVKAVKI
GMLSQTAVIAAVAEGLARRAGAIPVVLDPVMVATSGDRLITDAAVATLRERLLPRADLITPNLPEAAVLLGEPVAADAAA
IVAQARRLVALGARAVLIKGGHGTGRESIDHLVTADGALRTLSAPRIATRNTHGTGCTLSAAVAAGLAQGLALPEAVAEA
KAYLTAALAAADSLAVGTGHGPVHHFHAVWS

Sequences:

>Translated_271_residues
MSGPAPIAVTIAGSDSGGGAGIQADLKTFSALGVYGASVITALTAQNTIGVQGIHDVPADFVARQIDSVFADLAVKAVKI
GMLSQTAVIAAVAEGLARRAGAIPVVLDPVMVATSGDRLITDAAVATLRERLLPRADLITPNLPEAAVLLGEPVAADAAA
IVAQARRLVALGARAVLIKGGHGTGRESIDHLVTADGALRTLSAPRIATRNTHGTGCTLSAAVAAGLAQGLALPEAVAEA
KAYLTAALAAADSLAVGTGHGPVHHFHAVWS
>Mature_270_residues
SGPAPIAVTIAGSDSGGGAGIQADLKTFSALGVYGASVITALTAQNTIGVQGIHDVPADFVARQIDSVFADLAVKAVKIG
MLSQTAVIAAVAEGLARRAGAIPVVLDPVMVATSGDRLITDAAVATLRERLLPRADLITPNLPEAAVLLGEPVAADAAAI
VAQARRLVALGARAVLIKGGHGTGRESIDHLVTADGALRTLSAPRIATRNTHGTGCTLSAAVAAGLAQGLALPEAVAEAK
AYLTAALAAADSLAVGTGHGPVHHFHAVWS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=263, Percent_Identity=58.5551330798479, Blast_Score=288, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6325378, Length=276, Percent_Identity=33.695652173913, Blast_Score=145, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=35.2727272727273, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=275, Percent_Identity=34.9090909090909, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 26960; Mature: 26829

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPAPIAVTIAGSDSGGGAGIQADLKTFSALGVYGASVITALTAQNTIGVQGIHDVPAD
CCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FVARQIDSVFADLAVKAVKIGMLSQTAVIAAVAEGLARRAGAIPVVLDPVMVATSGDRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCHH
TDAAVATLRERLLPRADLITPNLPEAAVLLGEPVAADAAAIVAQARRLVALGARAVLIKG
HHHHHHHHHHHHCCCHHCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEC
GHGTGRESIDHLVTADGALRTLSAPRIATRNTHGTGCTLSAAVAAGLAQGLALPEAVAEA
CCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
KAYLTAALAAADSLAVGTGHGPVHHFHAVWS
HHHHHHHHHHHHHHEEECCCCCHHHHHEECC
>Mature Secondary Structure 
SGPAPIAVTIAGSDSGGGAGIQADLKTFSALGVYGASVITALTAQNTIGVQGIHDVPAD
CCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
FVARQIDSVFADLAVKAVKIGMLSQTAVIAAVAEGLARRAGAIPVVLDPVMVATSGDRLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCHH
TDAAVATLRERLLPRADLITPNLPEAAVLLGEPVAADAAAIVAQARRLVALGARAVLIKG
HHHHHHHHHHHHCCCHHCCCCCCCCHHHEECCCCHHHHHHHHHHHHHHHHHCCEEEEEEC
GHGTGRESIDHLVTADGALRTLSAPRIATRNTHGTGCTLSAAVAAGLAQGLALPEAVAEA
CCCCCHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
KAYLTAALAAADSLAVGTGHGPVHHFHAVWS
HHHHHHHHHHHHHHEEECCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]