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The map label for this gene is thiE [H]

Identifier: 170748377

GI number: 170748377

Start: 2089526

End: 2090272

Strand: Reverse

Name: thiE [H]

Synonym: Mrad2831_1959

Alternate gene names: 170748377

Gene position: 2090272-2089526 (Counterclockwise)

Preceding gene: 170748379

Following gene: 170748376

Centisome position: 34.39

GC content: 76.71

Gene sequence:

>747_bases
ATGCCAGCGAGCGCGACTTCCCCCGCGAATTCCCCTGCGAATTCCCCCGCGCCGTCCTCCGCGGCCGGCAGCCGGGCCGT
CGACCTGCGCCTCTACGGGCTCCTCGATGTCGGCGTCTGCGGCGCCGACGGGGCGCGGCTGGCGGAACTCGGCGCGGAGG
CGGTGGCGGGAGGCTGCACCCTGCTCCAGTACCGCGAGAAGGACATCGCCGATGCCCGCGCCGCCCTGGCGCGGATCCGG
GCGATCCTGGCAGCGGTGGGCGGCCGCGTCCCGGTCCTCGTCAACGACCGCGTCGACCTCGCCCTGGCGGCCGGAGCCGA
CGGGGTGCATCTCGGCCAGAGCGACCTCCACCCGGCCGACGCGCGCCGGCTCCTGGGGGAGGGCGCGATCATCGGCCTCA
CCCTGAAGAAGCCCGCCCAGGCCGACGAGCTGTACCGGCTGCCCGTCGACTACGCCTGCATCGGCGGCGTGTTCGCCACC
ACCAGCAAGGACAACCCCGACCCGCCGGTGGGCCTCGACGGCTTCGCCAGCATCGCCTTCCGCGCCCGCCTCGCCCGTGG
GGCCGGCCTGCCGCTCGGCGCCATCGCGGGGATCGGCCTCGACAACGCCGCCGCGGTCGTCGCCGCGGGGGCGGACGGCG
TCGCGGTCATCACCGCCCTGTTCGGCGCCGACTCGGTGCGCCAGCGCGCCCACGACCTGCGCGCGCGGATCGACGGCGCC
CTGGCGGAGCGGGCCGGCGCCCGATGA

Upstream 100 bases:

>100_bases
GCGCGCCGACGGCCGCTTGCCAACGCGGCGAGGCCTGCCCAAACAGGGGCCGGGGCGGGCCGGCACGGGGCCGGCCGAGG
CGGATCGAGCGACGACAGCG

Downstream 100 bases:

>100_bases
GCGGCCCGGCGCCCATCGCGGTCACGATCGCGGGCTCGGATTCCGGCGGCGGTGCCGGCATCCAGGCGGACCTCAAGACC
TTCTCGGCGCTGGGCGTCTA

Product: thiamine-phosphate diphosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MPASATSPANSPANSPAPSSAAGSRAVDLRLYGLLDVGVCGADGARLAELGAEAVAGGCTLLQYREKDIADARAALARIR
AILAAVGGRVPVLVNDRVDLALAAGADGVHLGQSDLHPADARRLLGEGAIIGLTLKKPAQADELYRLPVDYACIGGVFAT
TSKDNPDPPVGLDGFASIAFRARLARGAGLPLGAIAGIGLDNAAAVVAAGADGVAVITALFGADSVRQRAHDLRARIDGA
LAERAGAR

Sequences:

>Translated_248_residues
MPASATSPANSPANSPAPSSAAGSRAVDLRLYGLLDVGVCGADGARLAELGAEAVAGGCTLLQYREKDIADARAALARIR
AILAAVGGRVPVLVNDRVDLALAAGADGVHLGQSDLHPADARRLLGEGAIIGLTLKKPAQADELYRLPVDYACIGGVFAT
TSKDNPDPPVGLDGFASIAFRARLARGAGLPLGAIAGIGLDNAAAVVAAGADGVAVITALFGADSVRQRAHDLRARIDGA
LAERAGAR
>Mature_247_residues
PASATSPANSPANSPAPSSAAGSRAVDLRLYGLLDVGVCGADGARLAELGAEAVAGGCTLLQYREKDIADARAALARIRA
ILAAVGGRVPVLVNDRVDLALAAGADGVHLGQSDLHPADARRLLGEGAIIGLTLKKPAQADELYRLPVDYACIGGVFATT
SKDNPDPPVGLDGFASIAFRARLARGAGLPLGAIAGIGLDNAAAVVAAGADGVAVITALFGADSVRQRAHDLRARIDGAL
AERAGAR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=172, Percent_Identity=33.7209302325581, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325042, Length=231, Percent_Identity=29.8701298701299, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24822; Mature: 24691

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASATSPANSPANSPAPSSAAGSRAVDLRLYGLLDVGVCGADGARLAELGAEAVAGGCT
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHH
LLQYREKDIADARAALARIRAILAAVGGRVPVLVNDRVDLALAAGADGVHLGQSDLHPAD
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHH
ARRLLGEGAIIGLTLKKPAQADELYRLPVDYACIGGVFATTSKDNPDPPVGLDGFASIAF
HHHHHCCCCEEEEEECCCCCCCCEEECCCCHHHHCCEEEECCCCCCCCCCCCHHHHHHHH
RARLARGAGLPLGAIAGIGLDNAAAVVAAGADGVAVITALFGADSVRQRAHDLRARIDGA
HHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAERAGAR
HHHHCCCC
>Mature Secondary Structure 
PASATSPANSPANSPAPSSAAGSRAVDLRLYGLLDVGVCGADGARLAELGAEAVAGGCT
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHH
LLQYREKDIADARAALARIRAILAAVGGRVPVLVNDRVDLALAAGADGVHLGQSDLHPAD
EEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCHHH
ARRLLGEGAIIGLTLKKPAQADELYRLPVDYACIGGVFATTSKDNPDPPVGLDGFASIAF
HHHHHCCCCEEEEEECCCCCCCCEEECCCCHHHHCCEEEECCCCCCCCCCCCHHHHHHHH
RARLARGAGLPLGAIAGIGLDNAAAVVAAGADGVAVITALFGADSVRQRAHDLRARIDGA
HHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
LAERAGAR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]