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The map label for this gene is rlmL [H]

Identifier: 170683350

GI number: 170683350

Start: 2177642

End: 2179750

Strand: Reverse

Name: rlmL [H]

Synonym: EcSMS35_2171

Alternate gene names: 170683350

Gene position: 2179750-2177642 (Counterclockwise)

Preceding gene: 170683889

Following gene: 170681436

Centisome position: 43.01

GC content: 54.53

Gene sequence:

>2109_bases
ATGAATTCTCTGTTTGCCAGTACGGCCCGTGGGCTGGAAGAGCTGTTAAAAACTGAACTGGAAAACCTGGGGGCCGTTGA
ATGCCAGGTGGTTCAGGGTGGGGTCCATTTCAAGGGCGACACACGGCTTGTTTACCAGAGCCTGATGTGGAGCCGCCTGG
CCTCGCGTATTATGTTGCCGCTGGGCGAGTGTAAGGTTTACAGCGATTTAGACCTCTATCTCGGTGTTCAGGCGATCAAC
TGGACAGAGATGTTTAATCCTGGCGCGACCTTCGCTGTCCACTTCAGTGGTTTGAATGACACCATACGCAACAGTCAGTA
CGGTGCGATGAAAGTGAAAGACGCGATCGTCGATGCTTTCACGCGGAAAAATCTGCCGCGTCCAAATGTTGATCGCGATG
CGCCGGATATCCGCGTTAACGTCTGGCTGCATAAAGAAACTGCCAGTATCGCCCTTGATCTCAGTGGTGATGGTTTACAT
CTGCGTGGCTATCGCGATCGTGCTGGTATTGCGCCGATCAAAGAAACCCTGGCAGCCGCGATTGTTATGCGATCCGGCTG
GCAGCCAGGAACACCGCTGCTCGATCCGATGTGTGGTTCCGGTACGTTGCTGATTGAAGCGGCGATGCTGGCGACCGATC
GCGCACCAGGCTTGCACCGTGGACGTTGGGGCTTTAGCGGCTGGGCACAGCATGATGAAGCCATCTGGCAGGAAGTGAAA
GCAGAAGCGCAAACTCGCGCCCGTAAAGGCCTGGCGGAGTACAGCTCCCATTTCTACGGTTCGGACAGCGACGCACGGGT
GATTCAACGTGCGCGCACTAACGCCCGTCTTGCGGGGATTGGTGAACTGATCACCTTTGAGGTGAAAGATGTCGCGCAAC
TGGCCAATCCGCTGCCGAAAGGGCCGTACGGTACAGTGTTGAGCAACCCGCCATACGGTGAACGTCTGGACAGCGAACCG
GCGCTGATTGCGCTGCATAGTCTGCTGGGCCGAATCATGAAAAACCAGTTCGGTGGCTGGAATCTCTCTTTGTTTAGTGC
CTCGCCGGATCTGCTCAGCTGCCTGCAGCTGCGTGCAGACAAACAGTACAAGGCGAAAAACGGCCCGCTGGACTGCGTAC
AGAAAAACTACCATGTTGCCGAAAGCACACCAGACAGCAAACCGGCGATGGCAGCGGAGGACTACGCCAACCGTCTGCGT
AAGAACCTCAAAAAATTCGAGAAGTGGGCTCGTCAGGAAGGAATTGAATGTTACCGCCTGTATGACGCCGATCTGCCGGA
ATATAACGTTGCCGTTGACCGTTATGCCGACTGGGTGGTGGTGCAGGAGTATGCGCCGCCAAAAACTATTGATGCGCACA
AAGCGCGTCAGCGTCTGTTCGATATTATCGCTGCAACCATTTCGGTACTGGGGATTGCGCCAAACAAACTGGTACTGAAA
ACCCGTGAACGCCAGAAGGGCAAAAATCAGTACCAGAAACTGGGCGAGAAGGGCGAGTTTCTCGAAGTCACCGAATATAA
CGCTCACTTGTGGGTGAACCTGACGGATTACCTCGATACCGGTCTGTTCCTCGATCACCGTATCGCCCGTCGTATGCTCG
GTCAGATGAGCAAAGGCAAAGATTTCCTCAACCTGTTCTCTTATACCGGCAGCGCCACCGTGCACGCGGGATTAGGCGGT
GCACGCAGCACCACCACCGTGGATATGTCGCGTACTTATCTGGAGTGGGCAGAACGCAACCTGCGTCTGAATGGCTTAAC
CGGGCGTGCGCATCGCCTGATTCAGGCCGATTGCCTGGCGTGGTTGCGTGAGGCAAATGAACAGTTCGATCTGATCTTTA
TCGATCCGCCAACCTTTTCTAACTCAAAACGAATGGAAGATGCGTTTGATGTTCAGCGCGATCATCTGGTGCTGATGAAA
GATTTGAAACGTCTGCTGCGTGCAGGTGGGACGATCATGTTCTCGAACAACAAACGCGGCTTCCGTATGGATCTCGACGG
CCTGGCGAAACTGGGACTGAAAGCACAAGAAATTACGCAAAAAACGCTCTCCCAGGATTTCGCCCGTAACCGCCAGATCC
ACAACTGCTGGCTGATTACCGCAGCCTGA

Upstream 100 bases:

>100_bases
CACGGTGCGGTCCTCAAATGAAAATAAGCCCTCAACTTTATGACATGACGCGCTTATTAGCTATAATGCGCAACAATTTT
CTTAGAGTAAAAGTGACGAT

Downstream 100 bases:

>100_bases
AAGGAATAGTAATGTCATTAATCAGTATGCATGGCGCATGGCTGTCGTTCAGCGACGCGCCGCTTCTCGATAACGCAGAA
CTGCATATCGAAGATAACGA

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 702; Mature: 702

Protein sequence:

>702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLANPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLVLMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Sequences:

>Translated_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLANPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLVLMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
>Mature_702_residues
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAIN
WTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLANPLPKGPYGTVLSNPPYGERLDSEP
ALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAAEDYANRLR
KNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGG
ARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLVLMK
DLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=702, Percent_Identity=99.4301994301994, Blast_Score=1451, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=241, Percent_Identity=29.045643153527, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 78795; Mature: 78795

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLANPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMAAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCCEEHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
HHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLVLMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure
MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLVYQSLMWSRLASRIMLP
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEECCCHHHHHHHHHHHHHHHHHCCC
LGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTIRNSQYGAMKVKDAIVDAF
CCCCEEECCCCEEEEEEEECHHHHCCCCCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHH
TRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLHLRGYRDRAGIAPIKETLAAA
HHCCCCCCCCCCCCCCEEEEEEEEECCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHH
IVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGLHRGRWGFSGWAQHDEAIWQEVK
HHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
AEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNARLAGIGELITFEVKDVAQLANPLPK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHCCCCHHEEEEHHHHHHHHCCCCC
GPYGTVLSNPPYGERLDSEPALIALHSLLGRIMKNQFGGWNLSLFSASPDLLSCLQLRAD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCC
KQYKAKNGPLDCVQKNYHVAESTPDSKPAMAAEDYANRLRKNLKKFEKWARQEGIECYRL
CCCCCCCCCHHHHHCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
YDADLPEYNVAVDRYADWVVVQEYAPPKTIDAHKARQRLFDIIAATISVLGIAPNKLVLK
ECCCCCCCCEEHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TRERQKGKNQYQKLGEKGEFLEVTEYNAHLWVNLTDYLDTGLFLDHRIARRMLGQMSKGK
CHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEEHHHHCCCHHHHHHHHHHHHHHHHCCH
DFLNLFSYTGSATVHAGLGGARSTTTVDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLA
HHHHHHHCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHH
WLREANEQFDLIFIDPPTFSNSKRMEDAFDVQRDHLVLMKDLKRLLRAGGTIMFSNNKRG
HHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
FRMDLDGLAKLGLKAQEITQKTLSQDFARNRQIHNCWLITAA
EEECHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA