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The map label for this gene is malY [H]

Identifier: 170682367

GI number: 170682367

Start: 3032519

End: 3033694

Strand: Reverse

Name: malY [H]

Synonym: EcSMS35_2960

Alternate gene names: 170682367

Gene position: 3033694-3032519 (Counterclockwise)

Preceding gene: 170683282

Following gene: 170680141

Centisome position: 59.86

GC content: 37.93

Gene sequence:

>1176_bases
ATGGATGTTTTTAATACTCCTATTTCCCGCAAAGGAACATATTGTACCCAATGGGACTTTTGCGAGGACAGATTTGGTGT
AAAAGATGTATTACCATTCTCTATTTCAGATATGGATCTTCCGATACCAGACGCTATCACCAGAGCATTAAAAAAACGTC
TGGAACACCCGATTTTAGGGTACAGCCGTTGGCAGCATGATGACTATCTGAATGCGATAGTCAATTGGTATCATCAACAA
TATAAAACTGAAATCAAACCAGAATGGATCACCTATAGCCCAAGCGTGATGTACTCCATTGCTAAAGCAATAGAGTTATT
GACATCGCATGGTGATAATATTCTCGTTTTTACACCTGTCTATAATGCATTTTTTGATATAATTAAACATAGCGAGAGAA
ATATTCTGACAGCCTCTTTGATTATAAAAAATGAGGGGGGCTACGCTATCAATTGGCAAGATTTTGATCTTAAGATTCAA
AGCGCAAAGATGGTGTTATTATGTAATCCTCATAACCCGACAGGAACAGTCTGGTCGGAAGAAGAACTGCATAAAATCGC
TGCAAGCTGTACAAGGCATAATGTCTGGTTATGTTCAGATGAAATCCATAGTGATTTTGTTTTTAATCGTAGCTTTACAT
CCGCGCTTAAAATAAAAAAAGAAAAAGTAGTAGTATTTAACTCGATATCGAAAACATTTAATGTTCCTGCACTAACAGGA
TCGTATATGATTTCAACTGACGATAATTTTAATCATAAGTTCAGAACGATATCGAGGTATCGCGATTTTGTAAACTCCCC
ATCAGTACTGAATATAATTGCAACTATTATTGCCTATAACGAATGTGAGGGATGGTTAAAGTCATTAAAGGCACATATTG
CCTCTAACATTCAATTCACCCAGCAGTATCTGAACGAAAACACTCCAGAATTGATCGTAAGGCCAGCTGATGGTTGCTAT
TTTTCGTGGATAGATTGCTCTGCTATTGGTTATCCCTTTGATGAATTCTACAGTCGATTAATTCATGAAGGCAAAGTGGG
CATTATGGCGGGGAATGTCTATGGAACGGAGGGGGAGGGCTACCTTCGTCTGAACCTGGCCTGCGGTCGAGAAAAATTAT
ATATGGGGCTTACGCGTCTTGCGAGTGTAATTAAAAATATTAATCAAGGAAAATAA

Upstream 100 bases:

>100_bases
TTGCGGTTGTTAAACTTGATCAAAATACCCTACAAGTCATTATCGGTACTAAAGTCATCGCCTTGCGTCGGGATATGGAT
AACTACATGGGGATATGCTG

Downstream 100 bases:

>100_bases
AAATGAACAGCACGGATCTTATCCATCTCATTAAACATTTTATGCGTAACGAACATAAAGCAGTTGAAGAAGTTATTGAT
TCCCCCCTTTCGGAGGTCGC

Product: aminotransferase, classes I and II

Products: NA

Alternate protein names: Cystathionine beta-lyase MalY; CBL; Beta-cystathionase; Cysteine lyase; Maltose regulon modulator [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPDAITRALKKRLEHPILGYSRWQHDDYLNAIVNWYHQQ
YKTEIKPEWITYSPSVMYSIAKAIELLTSHGDNILVFTPVYNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQ
SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNVPALTG
SYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCY
FSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK

Sequences:

>Translated_391_residues
MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPDAITRALKKRLEHPILGYSRWQHDDYLNAIVNWYHQQ
YKTEIKPEWITYSPSVMYSIAKAIELLTSHGDNILVFTPVYNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQ
SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNVPALTG
SYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCY
FSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK
>Mature_391_residues
MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPDAITRALKKRLEHPILGYSRWQHDDYLNAIVNWYHQQ
YKTEIKPEWITYSPSVMYSIAKAIELLTSHGDNILVFTPVYNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQ
SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNVPALTG
SYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWLKSLKAHIASNIQFTQQYLNENTPELIVRPADGCY
FSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGNVYGTEGEGYLRLNLACGREKLYMGLTRLASVIKNINQGK

Specific function: Acts as a beta-cystathionase and as a repressor of the maltose regulon [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787909, Length=386, Percent_Identity=36.0103626943005, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1786816, Length=299, Percent_Identity=24.4147157190635, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994472, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71994476, Length=212, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17567663, Length=313, Percent_Identity=23.3226837060703, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322401, Length=319, Percent_Identity=23.8244514106583, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44844; Mature: 44844

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPDAITRALKKRLEHPILG
CCCCCCCCCCCCCEEECCCCHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
YSRWQHDDYLNAIVNWYHQQYKTEIKPEWITYSPSVMYSIAKAIELLTSHGDNILVFTPV
CHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCH
YNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQSAKMVLLCNPHNPTGTVWSE
HHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEECCEEEEECCEEEEEECCCCCCCCCCCH
EELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNVPALTG
HHHHHHHHHHHHCCEEEECCHHCCCEEECCCHHHHHHCCCCEEEEEECCHHHCCCCEECC
SYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWLKSLKAHIASNIQFT
CEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QQYLNENTPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGNVYGTEGEG
HHHHCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCEECCCCCC
YLRLNLACGREKLYMGLTRLASVIKNINQGK
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDVFNTPISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPDAITRALKKRLEHPILG
CCCCCCCCCCCCCEEECCCCHHHCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
YSRWQHDDYLNAIVNWYHQQYKTEIKPEWITYSPSVMYSIAKAIELLTSHGDNILVFTPV
CHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCEEEECCH
YNAFFDIIKHSERNILTASLIIKNEGGYAINWQDFDLKIQSAKMVLLCNPHNPTGTVWSE
HHHHHHHHHCCCCCEEEEEEEEECCCCEEEEEECCEEEEECCEEEEEECCCCCCCCCCCH
EELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKIKKEKVVVFNSISKTFNVPALTG
HHHHHHHHHHHHCCEEEECCHHCCCEEECCCHHHHHHCCCCEEEEEECCHHHCCCCEECC
SYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWLKSLKAHIASNIQFT
CEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
QQYLNENTPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEGKVGIMAGNVYGTEGEG
HHHHCCCCCEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCEECCCCCC
YLRLNLACGREKLYMGLTRLASVIKNINQGK
EEEEEEECCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1856179; 9097039; 9278503; 7665481; 10698925 [H]