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The map label for this gene is malX [H]

Identifier: 170683282

GI number: 170683282

Start: 3033694

End: 3035274

Strand: Reverse

Name: malX [H]

Synonym: EcSMS35_2961

Alternate gene names: 170683282

Gene position: 3035274-3033694 (Counterclockwise)

Preceding gene: 170680940

Following gene: 170682367

Centisome position: 59.89

GC content: 44.34

Gene sequence:

>1581_bases
ATGAAACAAAAGAAAGCCTGGAGTTTTTTTCAGAGCCTGGGAAAGGCATTCATGTATCCCATTGCTCTGCTAAGTGTATG
TGGCATGATGCTAGGGCTGGGAAGTGGTTTAGCCAGTGATGATATGGCAAAGTTAATTCCATTTCTGGCTATTCCAATAA
TTAAAACCATACTTGATTTCATTGTTAGTCTTGGTTTGTTTGCCTTTGTTAATTTACCTGTATTGTTTGCGATAGCGATT
CCCTTAGGATTATTAAAAGATAAAGAGGATAAAGCCTATGGTGCTTTTTCTGGCTTAATTGGTTTTATGGCGATGCATCT
GGGAACGAACTTTTATCTTAAACAGCACGACTTATTGGTCGCTGCTGACCAAATGTCGACACATGGGCAAACCATCATTC
TGGGGATCCAGTCCTACAATACCAGCGTGTTAGGGGGAATTGTTGCTGGGTTATTAGTCGCCAGCATGTATAAAAAGATC
GTTAATTTACGCATTCCTGAATCATTAGGTTTTTATAGCGGCCCACGTCTGGTGCCTATCATTACACTGATTGTGATGAG
TGGATTTGGTCTGATCATCCCTTTTATCTGGCCGCCGTTTTTCAATCTTTTCATGCTCATTGGCCACTGGATTTCAACTT
CCGGTCCTGTTGGCTATTTTTTCTATGCAGTCGCCGAACGCGTGACGATACCTTTTGGTTTAAATCACCTGGTGACGTCA
GTTTTCCGCTTTACCCCCATCGGCGGATCGGCAGTGATCGGCGGCGAAGAGTATTATGGCACCCTGAACATGTTTATGGC
GTACGTCAAAGAGAATGCGGTCATTCCGCTGGATTTGGCGGGGAAAATGGAACAGGGCAAACTGATGATTCAGTATGGTC
TGGCTGGTGCCGCGCTGGCGATGTATCGCACTGCTCATGCTCAAAACAGAAAAGCTATCAAAGCATTGCTTATTTCCGGG
GTGCTTACAGTCATTATTGGCGGCGTCAGCGAACCGATTGAGTTTCTGTTCTTATTTGTCAGTCCACTGCTGTTTGTCTT
CCATGCATTTATGAATGGATTCGCCAACATGGTCTTGCCATATATGGGCGTGAAGATGGGATTTACTGGCGATCTGATTC
AATTTATTAGCTTTGGCGTATTGCGTGGCACAAGAACAGGTTGGCCAATAGCGGTGTGTGTCGAAGTGGCCTATTTCTTT
ATTTATTACTTTGTGTTCCGTTGGACTATTCTCAAATTTAACCTGATGACGGTAGGCCGTGAAGAGTCCAGTCCTGTCAC
GCTGAACGCTCATGAGGATACGGCTATAGCGGATATCCCAACTCCTGATAAATCAGAGCTGCAAGCGGCGGAGCAGATGG
TTAAGGCACTTGGTGGTAAAGAGAATATTAAGTCACTGGATAATTGCGTAACTCGTTTACGTTTAACAATCGCAGATATG
GGATTGCTTGACGAGGCTGCAATAAAAAGAGCCGGCGGAATTGCGGTTGTTAAACTTGATCAAAATACCCTACAAGTCAT
TATCGGTACTAAAGTCATCGCCTTGCGTCGGGATATGGATAACTACATGGGGATATGCTGA

Upstream 100 bases:

>100_bases
TTTAAATATGAATTAATTTTAGAAGAAAAACTATATCTCTCAATTCGTACCAAAGTATTAATGGACTAATTATATATAAC
ATTTATATGAGATAACCCAC

Downstream 100 bases:

>100_bases
TGGATGTTTTTAATACTCCTATTTCCCGCAAAGGAACATATTGTACCCAATGGGACTTTTGCGAGGACAGATTTGGTGTA
AAAGATGTATTACCATTCTC

Product: PTS system, maltose and glucose-specific IIBC component family protein

Products: NA

Alternate protein names: Maltose and glucose permease IIC component; PTS system maltose- and glucose-specific EIIC component; Maltose- and glucose-specific phosphotransferase enzyme IIB component; PTS system maltose- and glucose-specific EIIB component [H]

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAI
PLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLNHLVTS
VFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISG
VLTVIIGGVSEPIEFLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFF
IYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLDNCVTRLRLTIADM
GLLDEAAIKRAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGIC

Sequences:

>Translated_526_residues
MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAI
PLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLNHLVTS
VFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISG
VLTVIIGGVSEPIEFLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFF
IYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLDNCVTRLRLTIADM
GLLDEAAIKRAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGIC
>Mature_526_residues
MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAKLIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAI
PLGLLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLNHLVTS
VFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISG
VLTVIIGGVSEPIEFLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAVCVEVAYFF
IYYFVFRWTILKFNLMTVGREESSPVTLNAHEDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLDNCVTRLRLTIADM
GLLDEAAIKRAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGIC

Specific function: MalX encodes a phosphotransferase system enzyme II that can recognize glucose and maltose as substrates even though these sugars may not represent the natural substrates of the system [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787908, Length=523, Percent_Identity=41.6826003824092, Blast_Score=410, Evalue=1e-116,
Organism=Escherichia coli, GI1787343, Length=523, Percent_Identity=32.6959847036329, Blast_Score=251, Evalue=9e-68,
Organism=Escherichia coli, GI1786894, Length=509, Percent_Identity=30.8447937131631, Blast_Score=192, Evalue=6e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011301 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 57592; Mature: 57592

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CHHHHHHHHHCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1856179; 9097039; 9278503; 2670898 [H]