Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 170079140

GI number: 170079140

Start: 2652094

End: 2654736

Strand: Reverse

Name: topA [H]

Synonym: SYNPCC7002_A2546

Alternate gene names: 170079140

Gene position: 2654736-2652094 (Counterclockwise)

Preceding gene: 170079146

Following gene: 170079139

Centisome position: 88.25

GC content: 51.57

Gene sequence:

>2643_bases
ATGTCTACCCTTGTCATCGTTGAATCCCCGACCAAAGCCCGTACCATTCGGAACTATCTACCGAAGGGATATCATGTGAC
GGCATCCATGGGCCATATCCGCGATTTGCCCTCCTCTGCCGAGGAAATCCCTAAGGAACAAAAAGGGCAGCCCTGGTCTC
AGCTTGGGGTCAATGTGGAAAATAACTTCGCGCCCCTCTACATCGTGCCGAAGAAAAAGAAGAAAGTTGTCACCGAACTC
AAGCAAGCCCTCAAAGAAGCCGATGAGTTAATCCTGGCGACGGACGAAGACCGCGAAGGGGAAAGTATTAGCTGGCATTT
GCTCGAAGTGCTCAAGCCCAAGGTGCCGATTAAACGGATGGTCTTCCATGAGATTACCCAAGAGGCGATCCAAAAAGCTT
TAACCAATTGCCGGGAAATTGACGAAAACCTCGTCCATGCCCAGGAAACCCGCCGGATTTTAGACCGTTTAGTGGGCTAC
ACTCTCTCGCCTTTACTCTGGCGCAAAATTGCCAAGGGTCTCTCGGCGGGACGGGTACAATCGGTGTCTGTACGCTTGTT
GGTGCAACGGGAACGGGAACGGCAGGCCTTTCAATCGGCTAACTATTGGGACCTCAAGGCAGAATTACTCCAGAAGAAAT
CAAAATTTGAGGCCAAGCTGATCACCCTCGGTGGCAAGAAATTAGCGTCTGGGAGTGACTTTGATGCGGCCACGGGGAAA
CTCAGCAAGGGTAAAAAAGTTGTCGTTCTCTCCCAAGCAGAGGCGATCGCCCTCAAGGATCGCCTAAAAGATCAACCTTG
GACGGTGAGCAAAACCGAGGAAAAACCGAGCACCCGCCGCCCCTCGCCCCCCTTTACCACTTCCACTCTGCAACAGGAAG
CGAACCGCAAACTCGGTATTTCGGCGCGGGATACCATGCGCACTGCCCAGAAGCTCTACGAAGAGGGCTATATCACCTAC
ATGCGGACGGACTCGGTGCACCTCTCGGAACAGGCGATCGCCGCAGCCCGTAGTTGCGTTGCAGAAAAATACGGCACAGA
ATATCTCAGTCCCAAGGTGCGCCAATATAAAACGAAAAGTAAAGGTGCCCAGGAAGCCCACGAAGCGATCCGGCCTGCGG
GCAATACCTTTCGCACGCCCCAGGAAACCGGCCTCAGTGGTCGAGAGTTAGCCCTTTATGACCTTATTTGGAAACGCACT
GTTGCCTGTCAGATGGCCGATGCTCGTCTCACTCAACTGTCGGTCATTCTCAAAGTCGATGATGCTGAGTTCCGCTCTGC
TGGTAAACGCATTGATTTTCCAGGTTTTTTCCGGGCCTATGTGGAAGGCTCTGATGATCCCGATGCTGCCCTCGAAAACC
AAGAAGTGGTGCTCCCCGATCTCAAGGAAGGAGACCAGCCCACCTGCAAAAAATTAGAAGAAGTCGATCACAATACTCAA
CCCCCGGCCCGTTATACCGAGGCCAGTTTGGTCAAAGCCCTTGAAAGCAAAGGCATTGGTCGCCCCAGCACCTACGCCAC
AATTATCGGCACCATCGTTGATCGCGGTTATGTGCAAATTCGCGATAAAGCCTTAACTCCCACCTTTACGGCCTTTGCGG
TGACGGCCCTTCTGGAGGAACATTTCCCCAATCTGGTGGATACAGATTTCACCTCCCAAATGGAGCAGACCCTCGATGAG
ATTGCCACCGGCAAAGCCCAATGGTTGCCCTATCTAAAAAATTTCTACCTCGGAGATGATGGCCTGGGTACCCAAGTAAA
AGTGCGCGAGGAAAATATTGATCCGGCCACCGCGAAGGCGATCGCCATCGAAAACCTCCCCGTGAAAGTCAAAATTGGTC
GCTTTGGCCCCTACATTGAAGCTGCTAACGGCGAAGAAACAATTACCGCTTCCCTGCCCGCTGATTTAACCCCCGCTGAC
CTCGATCCGGAGCAGGTTGAGACAATTCTCAAACAAAAAACCGAAGGCCCCGAGCAATTGGGGACGCACCCTGAAACAGG
CGATCCCATCTATTTACTTGTGGGTAGCTATGGCCCCTACGTTCAACTGGGCGAAGTCACCGAAGAGAAGAAAAAGCCCA
AGCGTGCTTCCCTCCCCAAAACCGTTAAACCAGAAGAGCTCACCCTACAGATGGCTGTGGATCTGTTAGCGTTGCCGCGC
CTATTGGGAGAACATCCCGAAACCAACAAACCCGTTAAAGTGGGTCTCGGTCGCTTCGGCCCCTATGTTGTCCATGACCA
AGGGAAAGAAGGCAAAGATTACCGGTCTCTTAAAAAAGAAGACAATCTTTTTACGATCGATTTTGAACGGGCCATGGAAC
TATTAGCGCAACCAAAGCGTTCCCGTGGTCGGGGCAAAACAAAGACGCCCCTGAAGGAACTGGGGAAACATCCCGCGGAT
AACGAACCAGTCAATGTTTATGAAGGCCCCTATGGTATCTACGTGAATCACCTAAAAGTGAATGCAGGTTTGCCAGAAGG
AGAAACCGTAGAAACAATTACCCTCGAAAAGGCCCTCGAACTAATCGCCGAAAAAGCGGGTAGCAAAGCAACCAAGAAAC
GTTCGACGACAAAAAAGAAAACGACGACGAAAAAAACAACAGCAAAAAAAACCACCAGCCGTAAAACAAAAACTTCTTCA
TGA

Upstream 100 bases:

>100_bases
AATTTAATCCTTGACGAAACAGGGGCTTATGGGCCATTGATTAGATATGTAAGGTATATCAGAAGCCTAAACACCTAAAC
CCTCACTTTTTTGACAGCCC

Downstream 100 bases:

>100_bases
GCCACAGCAAGCGTCGGTGGGAAGATTGGGGCCAAACCCTTGGCGATCGCCAAATTATCTTAGGTTTAAGTCTAGCGGCC
CTTTTTTTGTTTTGTCTGAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MSTLVIVESPTKARTIRNYLPKGYHVTASMGHIRDLPSSAEEIPKEQKGQPWSQLGVNVENNFAPLYIVPKKKKKVVTEL
KQALKEADELILATDEDREGESISWHLLEVLKPKVPIKRMVFHEITQEAIQKALTNCREIDENLVHAQETRRILDRLVGY
TLSPLLWRKIAKGLSAGRVQSVSVRLLVQRERERQAFQSANYWDLKAELLQKKSKFEAKLITLGGKKLASGSDFDAATGK
LSKGKKVVVLSQAEAIALKDRLKDQPWTVSKTEEKPSTRRPSPPFTTSTLQQEANRKLGISARDTMRTAQKLYEEGYITY
MRTDSVHLSEQAIAAARSCVAEKYGTEYLSPKVRQYKTKSKGAQEAHEAIRPAGNTFRTPQETGLSGRELALYDLIWKRT
VACQMADARLTQLSVILKVDDAEFRSAGKRIDFPGFFRAYVEGSDDPDAALENQEVVLPDLKEGDQPTCKKLEEVDHNTQ
PPARYTEASLVKALESKGIGRPSTYATIIGTIVDRGYVQIRDKALTPTFTAFAVTALLEEHFPNLVDTDFTSQMEQTLDE
IATGKAQWLPYLKNFYLGDDGLGTQVKVREENIDPATAKAIAIENLPVKVKIGRFGPYIEAANGEETITASLPADLTPAD
LDPEQVETILKQKTEGPEQLGTHPETGDPIYLLVGSYGPYVQLGEVTEEKKKPKRASLPKTVKPEELTLQMAVDLLALPR
LLGEHPETNKPVKVGLGRFGPYVVHDQGKEGKDYRSLKKEDNLFTIDFERAMELLAQPKRSRGRGKTKTPLKELGKHPAD
NEPVNVYEGPYGIYVNHLKVNAGLPEGETVETITLEKALELIAEKAGSKATKKRSTTKKKTTTKKTTAKKTTSRKTKTSS

Sequences:

>Translated_880_residues
MSTLVIVESPTKARTIRNYLPKGYHVTASMGHIRDLPSSAEEIPKEQKGQPWSQLGVNVENNFAPLYIVPKKKKKVVTEL
KQALKEADELILATDEDREGESISWHLLEVLKPKVPIKRMVFHEITQEAIQKALTNCREIDENLVHAQETRRILDRLVGY
TLSPLLWRKIAKGLSAGRVQSVSVRLLVQRERERQAFQSANYWDLKAELLQKKSKFEAKLITLGGKKLASGSDFDAATGK
LSKGKKVVVLSQAEAIALKDRLKDQPWTVSKTEEKPSTRRPSPPFTTSTLQQEANRKLGISARDTMRTAQKLYEEGYITY
MRTDSVHLSEQAIAAARSCVAEKYGTEYLSPKVRQYKTKSKGAQEAHEAIRPAGNTFRTPQETGLSGRELALYDLIWKRT
VACQMADARLTQLSVILKVDDAEFRSAGKRIDFPGFFRAYVEGSDDPDAALENQEVVLPDLKEGDQPTCKKLEEVDHNTQ
PPARYTEASLVKALESKGIGRPSTYATIIGTIVDRGYVQIRDKALTPTFTAFAVTALLEEHFPNLVDTDFTSQMEQTLDE
IATGKAQWLPYLKNFYLGDDGLGTQVKVREENIDPATAKAIAIENLPVKVKIGRFGPYIEAANGEETITASLPADLTPAD
LDPEQVETILKQKTEGPEQLGTHPETGDPIYLLVGSYGPYVQLGEVTEEKKKPKRASLPKTVKPEELTLQMAVDLLALPR
LLGEHPETNKPVKVGLGRFGPYVVHDQGKEGKDYRSLKKEDNLFTIDFERAMELLAQPKRSRGRGKTKTPLKELGKHPAD
NEPVNVYEGPYGIYVNHLKVNAGLPEGETVETITLEKALELIAEKAGSKATKKRSTTKKKTTTKKTTAKKTTSRKTKTSS
>Mature_879_residues
STLVIVESPTKARTIRNYLPKGYHVTASMGHIRDLPSSAEEIPKEQKGQPWSQLGVNVENNFAPLYIVPKKKKKVVTELK
QALKEADELILATDEDREGESISWHLLEVLKPKVPIKRMVFHEITQEAIQKALTNCREIDENLVHAQETRRILDRLVGYT
LSPLLWRKIAKGLSAGRVQSVSVRLLVQRERERQAFQSANYWDLKAELLQKKSKFEAKLITLGGKKLASGSDFDAATGKL
SKGKKVVVLSQAEAIALKDRLKDQPWTVSKTEEKPSTRRPSPPFTTSTLQQEANRKLGISARDTMRTAQKLYEEGYITYM
RTDSVHLSEQAIAAARSCVAEKYGTEYLSPKVRQYKTKSKGAQEAHEAIRPAGNTFRTPQETGLSGRELALYDLIWKRTV
ACQMADARLTQLSVILKVDDAEFRSAGKRIDFPGFFRAYVEGSDDPDAALENQEVVLPDLKEGDQPTCKKLEEVDHNTQP
PARYTEASLVKALESKGIGRPSTYATIIGTIVDRGYVQIRDKALTPTFTAFAVTALLEEHFPNLVDTDFTSQMEQTLDEI
ATGKAQWLPYLKNFYLGDDGLGTQVKVREENIDPATAKAIAIENLPVKVKIGRFGPYIEAANGEETITASLPADLTPADL
DPEQVETILKQKTEGPEQLGTHPETGDPIYLLVGSYGPYVQLGEVTEEKKKPKRASLPKTVKPEELTLQMAVDLLALPRL
LGEHPETNKPVKVGLGRFGPYVVHDQGKEGKDYRSLKKEDNLFTIDFERAMELLAQPKRSRGRGKTKTPLKELGKHPADN
EPVNVYEGPYGIYVNHLKVNAGLPEGETVETITLEKALELIAEKAGSKATKKRSTTKKKTTTKKTTAKKTTSRKTKTSS

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=701, Percent_Identity=25.2496433666191, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI4507635, Length=578, Percent_Identity=23.1833910034602, Blast_Score=120, Evalue=8e-27,
Organism=Escherichia coli, GI1787529, Length=590, Percent_Identity=41.3559322033898, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1788061, Length=628, Percent_Identity=24.6815286624204, Blast_Score=136, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=567, Percent_Identity=26.1022927689594, Blast_Score=137, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32563869, Length=636, Percent_Identity=24.2138364779874, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=564, Percent_Identity=24.468085106383, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24585251, Length=467, Percent_Identity=26.5524625267666, Blast_Score=120, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24640096, Length=530, Percent_Identity=21.8867924528302, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98020; Mature: 97889

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVIVESPTKARTIRNYLPKGYHVTASMGHIRDLPSSAEEIPKEQKGQPWSQLGVNVE
CCEEEEEECCHHHHHHHHHCCCCEEEEECCHHHHCCCCCHHHCCHHHCCCCHHHHCCCCC
NNFAPLYIVPKKKKKVVTELKQALKEADELILATDEDREGESISWHLLEVLKPKVPIKRM
CCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITQEAIQKALTNCREIDENLVHAQETRRILDRLVGYTLSPLLWRKIAKGLSAGRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SVSVRLLVQRERERQAFQSANYWDLKAELLQKKSKFEAKLITLGGKKLASGSDFDAATGK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHCCC
LSKGKKVVVLSQAEAIALKDRLKDQPWTVSKTEEKPSTRRPSPPFTTSTLQQEANRKLGI
CCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
SARDTMRTAQKLYEEGYITYMRTDSVHLSEQAIAAARSCVAEKYGTEYLSPKVRQYKTKS
CHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHC
KGAQEAHEAIRPAGNTFRTPQETGLSGRELALYDLIWKRTVACQMADARLTQLSVILKVD
CCHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
DAEFRSAGKRIDFPGFFRAYVEGSDDPDAALENQEVVLPDLKEGDQPTCKKLEEVDHNTQ
CHHHHHCCCCCCCCHHHHHHCCCCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCC
PPARYTEASLVKALESKGIGRPSTYATIIGTIVDRGYVQIRDKALTPTFTAFAVTALLEE
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
HFPNLVDTDFTSQMEQTLDEIATGKAQWLPYLKNFYLGDDGLGTQVKVREENIDPATAKA
HCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCEEEEECCCCCCHHHHE
IAIENLPVKVKIGRFGPYIEAANGEETITASLPADLTPADLDPEQVETILKQKTEGPEQL
EEEECCCEEEEECCCCCEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
GTHPETGDPIYLLVGSYGPYVQLGEVTEEKKKPKRASLPKTVKPEELTLQMAVDLLALPR
CCCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLGEHPETNKPVKVGLGRFGPYVVHDQGKEGKDYRSLKKEDNLFTIDFERAMELLAQPKR
HHCCCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCCHH
SRGRGKTKTPLKELGKHPADNEPVNVYEGPYGIYVNHLKVNAGLPEGETVETITLEKALE
HCCCCCCCCHHHHHCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCEEHHHHHHHHH
LIAEKAGSKATKKRSTTKKKTTTKKTTAKKTTSRKTKTSS
HHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STLVIVESPTKARTIRNYLPKGYHVTASMGHIRDLPSSAEEIPKEQKGQPWSQLGVNVE
CEEEEEECCHHHHHHHHHCCCCEEEEECCHHHHCCCCCHHHCCHHHCCCCHHHHCCCCC
NNFAPLYIVPKKKKKVVTELKQALKEADELILATDEDREGESISWHLLEVLKPKVPIKRM
CCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHHH
VFHEITQEAIQKALTNCREIDENLVHAQETRRILDRLVGYTLSPLLWRKIAKGLSAGRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
SVSVRLLVQRERERQAFQSANYWDLKAELLQKKSKFEAKLITLGGKKLASGSDFDAATGK
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHCCC
LSKGKKVVVLSQAEAIALKDRLKDQPWTVSKTEEKPSTRRPSPPFTTSTLQQEANRKLGI
CCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
SARDTMRTAQKLYEEGYITYMRTDSVHLSEQAIAAARSCVAEKYGTEYLSPKVRQYKTKS
CHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHC
KGAQEAHEAIRPAGNTFRTPQETGLSGRELALYDLIWKRTVACQMADARLTQLSVILKVD
CCHHHHHHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEC
DAEFRSAGKRIDFPGFFRAYVEGSDDPDAALENQEVVLPDLKEGDQPTCKKLEEVDHNTQ
CHHHHHCCCCCCCCHHHHHHCCCCCCCCHHCCCCCEECCCCCCCCCHHHHHHHHHCCCCC
PPARYTEASLVKALESKGIGRPSTYATIIGTIVDRGYVQIRDKALTPTFTAFAVTALLEE
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
HFPNLVDTDFTSQMEQTLDEIATGKAQWLPYLKNFYLGDDGLGTQVKVREENIDPATAKA
HCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCCCCEEEEECCCCCCHHHHE
IAIENLPVKVKIGRFGPYIEAANGEETITASLPADLTPADLDPEQVETILKQKTEGPEQL
EEEECCCEEEEECCCCCEEECCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHH
GTHPETGDPIYLLVGSYGPYVQLGEVTEEKKKPKRASLPKTVKPEELTLQMAVDLLALPR
CCCCCCCCEEEEEEECCCCCEEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LLGEHPETNKPVKVGLGRFGPYVVHDQGKEGKDYRSLKKEDNLFTIDFERAMELLAQPKR
HHCCCCCCCCCEEEECCCCCCEEEECCCCCCCHHHHHHCCCCEEEEEHHHHHHHHHCCHH
SRGRGKTKTPLKELGKHPADNEPVNVYEGPYGIYVNHLKVNAGLPEGETVETITLEKALE
HCCCCCCCCHHHHHCCCCCCCCCCEEECCCCEEEEEEEEEECCCCCCCCEEHHHHHHHHH
LIAEKAGSKATKKRSTTKKKTTTKKTTAKKTTSRKTKTSS
HHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]