Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 170078806

GI number: 170078806

Start: 2298265

End: 2299863

Strand: Direct

Name: amtB [C]

Synonym: SYNPCC7002_A2208

Alternate gene names: 170078806

Gene position: 2298265-2299863 (Clockwise)

Preceding gene: 170078805

Following gene: 170078807

Centisome position: 76.4

GC content: 50.16

Gene sequence:

>1599_bases
ATGCCTAAACAACATCTCAAACTAATGCCTAAGTCAACTTTTCAACAACGTGGATGGGCACGGGAAGACATGCGGTCTCC
CCACACAACAGATAACAGTGTGGTGCTTCGCTTTGCCCGTCAGCTCTCCCGTCACTTCTCCCCTGCCTGGCTTGCCTGTA
TTCCCTTAGCGGCGATTATCACCGTCGTCTGGGGAACTGCAGCAATGGCACAATCCGACGCCGTCGATGCCGCAGGTCAA
GTCCAAATTATCCTAGACACGATCTTCCTGTTGATTGCGTCCGTCCTTGTTATTTTCATGAATGCAGGGTTCGGAATGCT
TGAAGCTGGTTTTTGTCGTCAAAAAAATGCGGTCAATATCCTCTCCAAGAACTTGATTGTCTTCGCCGTTGCCACCATTG
CTTATTGGGCGGTTGGTTATGCCTTTATGTATGGCGATGGAAATACTTTTATCGGTCTGAAGGGCTTTTTCTTCAACGGT
AGTAGTATTCCCTACAGTGGAGATGGTAGTGAATGGTACATCGAAGAAGTTATTGATGGTGAAACAGTTCAAATTCTTCA
GTTCGTACCGGAAAGTGTCTCCTTCCTGTTCCAGGTTGCCTTCGCCGCCACCGCTGCCACCATCGTCTCTGGGGCTGTCG
CTGAGAGGATTAAATTCGACGCTTTCTTGATTTTCAGCTTCCTTTTAGTGGCGATTTCCTATCCCATTACAGGTCACTGG
CAGTGGGATGGCGAATGGCTGTCTGCACTTCCTTTCCTCGGTGTGGATGAAGAAGGGGCTGCTTTATTTGGTTTCCATGA
TTTTGCTGGTTCAACCATTGTTCACTCCGTTGGTGGTTGGGCTGCCCTTGTGGGTGCTGCAGTAGTCGGGCCTCGCCTCG
GCAAATACCGTGATGGTCAAGTCCACGCCATTCCTGGTCACAACATGAGTATTGCGACCTTAGGCTGTCTAATTCTTTGG
ATTGGCTGGTTTGGTTTTAACCCCGGTTCTCAATTGGCAGCAGATGCTGCGGTGCCTTACATCGCAATCACTACAAACCT
TTCGGCTGCAGCTGGGGGAATCACCGCAACCGCAACCTCTTGGATCAAAGATGGGAAGCCAGACCTGTCTATGATTATTA
ACGGTATTTTGGCTGGTCTCGTTGGGATTACAGCCGGTTGTGATGGCGTCAGTTTCTTTTCTGCTGTGATCATCGGGGCG
ATCGCCGGTGTACTCGTCGTCTTCTCTGTGGCCTTCTTCGATGCTATTAAAATCGATGACCCCGTTGGTGCGACCTCTGT
ACACCTCGTCTGCGGTATCTGGGGAACTCTTGCCGTTGGTCTGTTCAAGATGGATGGGGGTTTATTCACTGGCGGTGGCA
TCCAACAGCTGATTGCCCAAATCGTCGGAATTCTTTCCATTGGTGGCTTTACCGTCGCCTTTAGCTTTATTGTTTGGTAT
GCCCTATCGGCAGTCCTTGGTGGAATTCGCGTCGAAAAAGACGAGGAACTCCGGGGTCTCGACATTGGTGAGCACGGCAT
GGAAGCTTACAGCGGCTTTGTTAAAGAGTCCGATGTTATCTTCCGAGGGACTGCCACTGGTTCCGAAACCGAAGGATAA

Upstream 100 bases:

>100_bases
CAGACCATAGATGATTTTTCGGCAACTTTCGTTGATGGCTTTTCAAAAGGTTACTTCTGCTACCATTAGCGAATTTTGCC
TGTGTTATTCCATTGAGTGA

Downstream 100 bases:

>100_bases
ATCCTCCCAGGAAATCCTTAAAACAATCTAAAGAAATTTTTCCTAACCTTCCTTACCCAAGGGAGGTTTTTTATGTGAGT
TCACATTTTGTTACGTTACC

Product: ammonium/methylammonium permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MPKQHLKLMPKSTFQQRGWAREDMRSPHTTDNSVVLRFARQLSRHFSPAWLACIPLAAIITVVWGTAAMAQSDAVDAAGQ
VQIILDTIFLLIASVLVIFMNAGFGMLEAGFCRQKNAVNILSKNLIVFAVATIAYWAVGYAFMYGDGNTFIGLKGFFFNG
SSIPYSGDGSEWYIEEVIDGETVQILQFVPESVSFLFQVAFAATAATIVSGAVAERIKFDAFLIFSFLLVAISYPITGHW
QWDGEWLSALPFLGVDEEGAALFGFHDFAGSTIVHSVGGWAALVGAAVVGPRLGKYRDGQVHAIPGHNMSIATLGCLILW
IGWFGFNPGSQLAADAAVPYIAITTNLSAAAGGITATATSWIKDGKPDLSMIINGILAGLVGITAGCDGVSFFSAVIIGA
IAGVLVVFSVAFFDAIKIDDPVGATSVHLVCGIWGTLAVGLFKMDGGLFTGGGIQQLIAQIVGILSIGGFTVAFSFIVWY
ALSAVLGGIRVEKDEELRGLDIGEHGMEAYSGFVKESDVIFRGTATGSETEG

Sequences:

>Translated_532_residues
MPKQHLKLMPKSTFQQRGWAREDMRSPHTTDNSVVLRFARQLSRHFSPAWLACIPLAAIITVVWGTAAMAQSDAVDAAGQ
VQIILDTIFLLIASVLVIFMNAGFGMLEAGFCRQKNAVNILSKNLIVFAVATIAYWAVGYAFMYGDGNTFIGLKGFFFNG
SSIPYSGDGSEWYIEEVIDGETVQILQFVPESVSFLFQVAFAATAATIVSGAVAERIKFDAFLIFSFLLVAISYPITGHW
QWDGEWLSALPFLGVDEEGAALFGFHDFAGSTIVHSVGGWAALVGAAVVGPRLGKYRDGQVHAIPGHNMSIATLGCLILW
IGWFGFNPGSQLAADAAVPYIAITTNLSAAAGGITATATSWIKDGKPDLSMIINGILAGLVGITAGCDGVSFFSAVIIGA
IAGVLVVFSVAFFDAIKIDDPVGATSVHLVCGIWGTLAVGLFKMDGGLFTGGGIQQLIAQIVGILSIGGFTVAFSFIVWY
ALSAVLGGIRVEKDEELRGLDIGEHGMEAYSGFVKESDVIFRGTATGSETEG
>Mature_531_residues
PKQHLKLMPKSTFQQRGWAREDMRSPHTTDNSVVLRFARQLSRHFSPAWLACIPLAAIITVVWGTAAMAQSDAVDAAGQV
QIILDTIFLLIASVLVIFMNAGFGMLEAGFCRQKNAVNILSKNLIVFAVATIAYWAVGYAFMYGDGNTFIGLKGFFFNGS
SIPYSGDGSEWYIEEVIDGETVQILQFVPESVSFLFQVAFAATAATIVSGAVAERIKFDAFLIFSFLLVAISYPITGHWQ
WDGEWLSALPFLGVDEEGAALFGFHDFAGSTIVHSVGGWAALVGAAVVGPRLGKYRDGQVHAIPGHNMSIATLGCLILWI
GWFGFNPGSQLAADAAVPYIAITTNLSAAAGGITATATSWIKDGKPDLSMIINGILAGLVGITAGCDGVSFFSAVIIGAI
AGVLVVFSVAFFDAIKIDDPVGATSVHLVCGIWGTLAVGLFKMDGGLFTGGGIQQLIAQIVGILSIGGFTVAFSFIVWYA
LSAVLGGIRVEKDEELRGLDIGEHGMEAYSGFVKESDVIFRGTATGSETEG

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=463, Percent_Identity=31.5334773218143, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32564042, Length=446, Percent_Identity=32.2869955156951, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17550182, Length=458, Percent_Identity=34.2794759825328, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI133931100, Length=453, Percent_Identity=29.8013245033113, Blast_Score=191, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17550180, Length=454, Percent_Identity=30.8370044052863, Blast_Score=191, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324187, Length=449, Percent_Identity=28.5077951002227, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6325396, Length=440, Percent_Identity=27.9545454545455, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321559, Length=459, Percent_Identity=27.2331154684096, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI161078314, Length=440, Percent_Identity=37.7272727272727, Blast_Score=225, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 56391; Mature: 56259

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKQHLKLMPKSTFQQRGWAREDMRSPHTTDNSVVLRFARQLSRHFSPAWLACIPLAAII
CCCCHHHCCCCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVVWGTAAMAQSDAVDAAGQVQIILDTIFLLIASVLVIFMNAGFGMLEAGFCRQKNAVNI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHCCCCHHHH
LSKNLIVFAVATIAYWAVGYAFMYGDGNTFIGLKGFFFNGSSIPYSGDGSEWYIEEVIDG
HHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCC
ETVQILQFVPESVSFLFQVAFAATAATIVSGAVAERIKFDAFLIFSFLLVAISYPITGHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
QWDGEWLSALPFLGVDEEGAALFGFHDFAGSTIVHSVGGWAALVGAAVVGPRLGKYRDGQ
EECCHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCE
VHAIPGHNMSIATLGCLILWIGWFGFNPGSQLAADAAVPYIAITTNLSAAAGGITATATS
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCHHCCCCHHHHHH
WIKDGKPDLSMIINGILAGLVGITAGCDGVSFFSAVIIGAIAGVLVVFSVAFFDAIKIDD
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVGATSVHLVCGIWGTLAVGLFKMDGGLFTGGGIQQLIAQIVGILSIGGFTVAFSFIVWY
CCCCCHHHHHHHHHHHHHHHHHEECCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALSAVLGGIRVEKDEELRGLDIGEHGMEAYSGFVKESDVIFRGTATGSETEG
HHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCC
>Mature Secondary Structure 
PKQHLKLMPKSTFQQRGWAREDMRSPHTTDNSVVLRFARQLSRHFSPAWLACIPLAAII
CCCHHHCCCCHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TVVWGTAAMAQSDAVDAAGQVQIILDTIFLLIASVLVIFMNAGFGMLEAGFCRQKNAVNI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCHHCCCCHHHH
LSKNLIVFAVATIAYWAVGYAFMYGDGNTFIGLKGFFFNGSSIPYSGDGSEWYIEEVIDG
HHHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCHHHHHHCC
ETVQILQFVPESVSFLFQVAFAATAATIVSGAVAERIKFDAFLIFSFLLVAISYPITGHW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
QWDGEWLSALPFLGVDEEGAALFGFHDFAGSTIVHSVGGWAALVGAAVVGPRLGKYRDGQ
EECCHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCE
VHAIPGHNMSIATLGCLILWIGWFGFNPGSQLAADAAVPYIAITTNLSAAAGGITATATS
EEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCHHCCCCHHHHHH
WIKDGKPDLSMIINGILAGLVGITAGCDGVSFFSAVIIGAIAGVLVVFSVAFFDAIKIDD
HHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PVGATSVHLVCGIWGTLAVGLFKMDGGLFTGGGIQQLIAQIVGILSIGGFTVAFSFIVWY
CCCCCHHHHHHHHHHHHHHHHHEECCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
ALSAVLGGIRVEKDEELRGLDIGEHGMEAYSGFVKESDVIFRGTATGSETEG
HHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]