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The map label for this gene is miaA

Identifier: 170077812

GI number: 170077812

Start: 1239527

End: 1240423

Strand: Reverse

Name: miaA

Synonym: SYNPCC7002_A1195

Alternate gene names: 170077812

Gene position: 1240423-1239527 (Counterclockwise)

Preceding gene: 170077813

Following gene: 170077809

Centisome position: 41.24

GC content: 52.62

Gene sequence:

>897_bases
ATGACTGCCCCCCTCATCGTCATCTGTGGTGCCACCGCGACGGGTAAATCCGGTTTGGCCCTCAAACTTGCCGAAAAACT
GGACGCGGTAATTCTTAGCGCCGATTCGCGTCAAATTTACAAAGAATTCGACATCGGTACAGCGAAACCAACCCCTGCAG
AGCAGCAAAGCATTCCCCATTACCTGATCGATATCTGCGAACCGACAGAAACGCTCACCTTGGCGGAATATCAAGCCCAG
GCCCAAGCACTAATCGCGCAATTTCACGCCGAACAGAAACCCGTTTTACTCGTCGGCGGCACTGGCCTTTACATTAAAGC
GATTACCAAAGGTTTAAAAATTCCCCGGGTTGCGCCTCAACCGGATCTGCGCCAACAATTCACCGACCTGGGCCAAGCCT
ATAGCTACCAACTGCTCCGGCAGGTTGACCCAGAAGTTTGTCAGAAAATTCACCCCAATGACCAAGTGCGTACCCTCCGG
GCCCTAGAGGTTTTTTATGTCACTGGGGAGCCGATTAGCAGCCAACAGGGAGAAAATTCGCCGACTTATTCGATCCTCGA
AATTGGTCTCGATTGCGCTCCCGACCACCTCGATCAACGGATCCGTCAACGCACCCAGCAGATGGTTGACCAAGGCTTTG
TTGCTGAGGTTGAACGCTTGGGTAAAAAATACGGTTGGGATCTGCCCCTCCTGAACACCCTCGGTTATGCGGAATTTCGG
GATTATGTACGCGGTCAAGGGACATTAGAAGAGGCGATCGCCAACACCGTGCTCCACACCCGCCAATTTGCCAAACGCCA
ACGCACTTGGTTCCGCGCCAATCCAGATATTCACTGGCTCGATAACACCGCCCCAGATCTCCTTGAGCAAGCCCTCAATC
TCATTACTCAACAATAG

Upstream 100 bases:

>100_bases
GGCACGATCGTCGAGCGCATCGATAAGTTCCAAAACCGCGATCGCCTTAATGCCCAGCTCGAACAACTCCTCCAGGGCGT
GGTGATCCCCTAAATTTTTT

Downstream 100 bases:

>100_bases
AGATTTGAAAGATTTTCCACATAAAAAAGCACCCTTTCGCGAGAAAGAATGCTTCAACGAATTTAAGGTTGAAAAATCAG
ACAAACATTAAGGACGTTCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTAPLIVICGATATGKSGLALKLAEKLDAVILSADSRQIYKEFDIGTAKPTPAEQQSIPHYLIDICEPTETLTLAEYQAQ
AQALIAQFHAEQKPVLLVGGTGLYIKAITKGLKIPRVAPQPDLRQQFTDLGQAYSYQLLRQVDPEVCQKIHPNDQVRTLR
ALEVFYVTGEPISSQQGENSPTYSILEIGLDCAPDHLDQRIRQRTQQMVDQGFVAEVERLGKKYGWDLPLLNTLGYAEFR
DYVRGQGTLEEAIANTVLHTRQFAKRQRTWFRANPDIHWLDNTAPDLLEQALNLITQQ

Sequences:

>Translated_298_residues
MTAPLIVICGATATGKSGLALKLAEKLDAVILSADSRQIYKEFDIGTAKPTPAEQQSIPHYLIDICEPTETLTLAEYQAQ
AQALIAQFHAEQKPVLLVGGTGLYIKAITKGLKIPRVAPQPDLRQQFTDLGQAYSYQLLRQVDPEVCQKIHPNDQVRTLR
ALEVFYVTGEPISSQQGENSPTYSILEIGLDCAPDHLDQRIRQRTQQMVDQGFVAEVERLGKKYGWDLPLLNTLGYAEFR
DYVRGQGTLEEAIANTVLHTRQFAKRQRTWFRANPDIHWLDNTAPDLLEQALNLITQQ
>Mature_297_residues
TAPLIVICGATATGKSGLALKLAEKLDAVILSADSRQIYKEFDIGTAKPTPAEQQSIPHYLIDICEPTETLTLAEYQAQA
QALIAQFHAEQKPVLLVGGTGLYIKAITKGLKIPRVAPQPDLRQQFTDLGQAYSYQLLRQVDPEVCQKIHPNDQVRTLRA
LEVFYVTGEPISSQQGENSPTYSILEIGLDCAPDHLDQRIRQRTQQMVDQGFVAEVERLGKKYGWDLPLLNTLGYAEFRD
YVRGQGTLEEAIANTVLHTRQFAKRQRTWFRANPDIHWLDNTAPDLLEQALNLITQQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=29.4871794871795, Blast_Score=124, Evalue=7e-29,
Organism=Escherichia coli, GI1790613, Length=301, Percent_Identity=38.2059800664452, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=25.0814332247557, Blast_Score=103, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6324848, Length=297, Percent_Identity=28.956228956229, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=30.7116104868914, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SYNP2 (B1XKE8)

Other databases:

- EMBL:   CP000951
- RefSeq:   YP_001734450.1
- ProteinModelPortal:   B1XKE8
- SMR:   B1XKE8
- GeneID:   6056131
- GenomeReviews:   CP000951_GR
- KEGG:   syp:SYNPCC7002_A1195
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33394; Mature: 33263

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPLIVICGATATGKSGLALKLAEKLDAVILSADSRQIYKEFDIGTAKPTPAEQQSIPH
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHCCCCCCCCCCHHHCCCH
YLIDICEPTETLTLAEYQAQAQALIAQFHAEQKPVLLVGGTGLYIKAITKGLKIPRVAPQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCCC
PDLRQQFTDLGQAYSYQLLRQVDPEVCQKIHPNDQVRTLRALEVFYVTGEPISSQQGENS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCCCC
PTYSILEIGLDCAPDHLDQRIRQRTQQMVDQGFVAEVERLGKKYGWDLPLLNTLGYAEFR
CCHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCHHHHH
DYVRGQGTLEEAIANTVLHTRQFAKRQRTWFRANPDIHWLDNTAPDLLEQALNLITQQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAPLIVICGATATGKSGLALKLAEKLDAVILSADSRQIYKEFDIGTAKPTPAEQQSIPH
CCCEEEEECCCCCCCCCCHHHHHHHHHHHEECCCHHHHHHHHCCCCCCCCCCHHHCCCH
YLIDICEPTETLTLAEYQAQAQALIAQFHAEQKPVLLVGGTGLYIKAITKGLKIPRVAPQ
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCCCCCCC
PDLRQQFTDLGQAYSYQLLRQVDPEVCQKIHPNDQVRTLRALEVFYVTGEPISSQQGENS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHEEEEEEECCCCCCCCCCCC
PTYSILEIGLDCAPDHLDQRIRQRTQQMVDQGFVAEVERLGKKYGWDLPLLNTLGYAEFR
CCHHHEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHCCHHHHH
DYVRGQGTLEEAIANTVLHTRQFAKRQRTWFRANPDIHWLDNTAPDLLEQALNLITQQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA