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The map label for this gene is cysJ [H]

Identifier: 170025657

GI number: 170025657

Start: 3774927

End: 3776729

Strand: Reverse

Name: cysJ [H]

Synonym: YPK_3442

Alternate gene names: 170025657

Gene position: 3776729-3774927 (Counterclockwise)

Preceding gene: 170025659

Following gene: 170025656

Centisome position: 80.54

GC content: 52.58

Gene sequence:

>1803_bases
ATGACGACTCAGGCTCCTCCAACTTCGTTACTTCCGCTATCGCCAGAGCAGTTGGCCCGCTTGCAAGCGGCGGTGGGTGA
ATTCTCCCCAACCCAAATGGCATGGTTGTCGGGTTACTTCTGGGGGATGGTTAACCAGCAGCCCGGCGCTGTTGCTTCCC
CTGCGGTAGCGGCACCCCCACCAGTGACAGTTACCCTGATTTCCGCGTCTCAAACCGGCAACGCCCGGCGTTTAGCTGAA
CAACTGCGCGATGATCTTCTGGCTGCCCAACTTAGTGTCAATCTGGTCAATGCGGGTGATTATAAGTTTAAACAAATTGC
GCAGGAGCGTTTACTGGTGGTGGTCGCATCGACCCAAGGAGAGGGGGAGCCAGCAGAAGAAGCCGTCGCACTGCATAAAT
TCCTGTTTTCTAAAAAAGCCCCGAAATTACCGGAGACCGCATTTGCTGTCTTTGGCTTAGGTGACACCTCTTACGAACAC
TTCTGTCAGGCTGGGAAAGATTTTGATAGCAAGCTGGCAGAACTTGGTGCTCAACGGTTACTGGATCGTGTCGATGCCGA
TGTTGAATATCAGGTACAAGCACAGCAGTGGCGTCAGCAAGTGGTTGCCACGTTGCAGGCTAAGGTTCCAGCACAATCTA
CAACTCCAGCAGCAGCGGCGATAACGTCTGGTGGTGCCACTACCGTTAGTCCGTACAGTAAAACCGCGCCGTTGACCGCA
CAGTTATCCGTACAGCAAAAAGTGACTGGGCGTAATTCAGAAAAAGATGTTCGTCATATTGAAATCGATTTAGGTGATTC
AGGACTGCGTTACCAACCAGGCGATGCTTTGGGTGTCTGGTTTGATAATGATCCTGCTTTGGTCGAAGAACTGCTCGCGC
TGTTGTGGCTCAAAGGTGATGAGCCGGTCAGTATTGACGGACAAAATATGCCACTGGCACAGGCGCTGCTCAGTCACCTT
GAACTGACACAAAATACGACGCTGATCGTCGATAAATACGCCGTGCTATCACGCGATGAAACCTTGATTGCACTGCTGGC
AGATAAACCCGCGTTGCAACTGTACGCTAAGAATACGCCATTCGTTGATATGGTGCGGCAGGCACCGTCTGATCTTAATG
CCGATCAACTGGTGGGGTTATTGCGCCCCTTAACGCCACGGTTGTACTCCATCGCCTCTTCGCAAGCTGAGACCGAAAAT
GAGGTGCATATCACCGTGGGTGTGGTGCGCTATGACATTGATGGCCGAGCGCGCAGTGGCGGTGCTTCCGGCTATCTGGC
GGACCGGCTGGAAGTCGATGGCGATATCCGTGTCTTTATTGAACACAATGATAATTTCCGTTTGCCTGCTAACCCGGAAA
CACCCGTCATCATGATCGGGCCAGGCACCGGTATCGCGCCGTTCCGCGCCTTTATGCAACAGCGTGAAGTTGATGGGGCC
AGTGGCAAGAACTGGTTATTCTTCGGTAATCCTCATTTCACCGAGGATTTTCTGTATCAGGTTGAATGGCAACGCTATGT
CAAAGAAGGCGTGTTGACGCGCATTGATCTGGCCTGGTCCCGTGATCAGGCGCATAAAATATACGTACAAGACAAACTGC
GCGAACAGGGCGCGGAACTGTGGAACTGGATACAACAAGGTGCCCACATTTACGTCTGTGGCGATGCTAACCGCATGGCG
AAAGACGTTGAGCAGGTTTTACTAGACGTGGTGGCTCTGCACGGTGCAATGGGTGCCGAGCAGGCTGATGAATATTTAAG
TGAGTTGCGCCAAGCGCGCCGCTATCAGCGAGATGTGTACTGA

Upstream 100 bases:

>100_bases
ACCTTACAAACTATCAAGACTTTGTGGCACGAATTGTCCTGCCTATATTGACAAATATTTGTGCCAACCACGGCTAAAGC
ATAAGGGAATTCGTACTGCA

Downstream 100 bases:

>100_bases
TGAATGAAAAACACCCAGGGCCACTCGTCGTCAGTGGAAAACTTTCTGATGGCGAGCGCATGAAGTCGGAAAGCAATTTC
TTGCGTGGGACTATTGCGGA

Product: sulfite reductase subunit alpha

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLPETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTTPAAAAITSGGATTVSPYSKTAPLTA
QLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQNMPLAQALLSHL
ELTQNTTLIVDKYAVLSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETEN
EVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQREVDGA
SGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGAHIYVCGDANRMA
KDVEQVLLDVVALHGAMGAEQADEYLSELRQARRYQRDVY

Sequences:

>Translated_600_residues
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAE
QLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLPETAFAVFGLGDTSYEH
FCQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTTPAAAAITSGGATTVSPYSKTAPLTA
QLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQNMPLAQALLSHL
ELTQNTTLIVDKYAVLSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETEN
EVHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQREVDGA
SGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGAHIYVCGDANRMA
KDVEQVLLDVVALHGAMGAEQADEYLSELRQARRYQRDVY
>Mature_599_residues
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPPPVTVTLISASQTGNARRLAEQ
LRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQGEGEPAEEAVALHKFLFSKKAPKLPETAFAVFGLGDTSYEHF
CQAGKDFDSKLAELGAQRLLDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTTPAAAAITSGGATTVSPYSKTAPLTAQ
LSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGDEPVSIDGQNMPLAQALLSHLE
LTQNTTLIVDKYAVLSRDETLIALLADKPALQLYAKNTPFVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENE
VHITVGVVRYDIDGRARSGGASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQREVDGAS
GKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAELWNWIQQGAHIYVCGDANRMAK
DVEQVLLDVVALHGAMGAEQADEYLSELRQARRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=602, Percent_Identity=31.5614617940199, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI24041029, Length=598, Percent_Identity=31.9397993311037, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI7657393, Length=598, Percent_Identity=29.2642140468227, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI221316709, Length=598, Percent_Identity=29.2642140468227, Blast_Score=206, Evalue=7e-53,
Organism=Homo sapiens, GI221316705, Length=607, Percent_Identity=28.830313014827, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI10835173, Length=652, Percent_Identity=28.3742331288344, Blast_Score=189, Evalue=9e-48,
Organism=Homo sapiens, GI221316707, Length=528, Percent_Identity=30.1136363636364, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI40254422, Length=650, Percent_Identity=28.3076923076923, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI169790956, Length=446, Percent_Identity=27.5784753363229, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI169790958, Length=446, Percent_Identity=27.5784753363229, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI1789123, Length=602, Percent_Identity=73.7541528239203, Blast_Score=917, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554134, Length=639, Percent_Identity=29.4209702660407, Blast_Score=247, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17566446, Length=573, Percent_Identity=24.956369982548, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531441, Length=443, Percent_Identity=25.5079006772009, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321832, Length=639, Percent_Identity=27.2300469483568, Blast_Score=196, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=31.2820512820513, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325305, Length=627, Percent_Identity=23.1259968102073, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137192, Length=600, Percent_Identity=29, Blast_Score=218, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24582192, Length=510, Percent_Identity=30.3921568627451, Blast_Score=199, Evalue=6e-51,
Organism=Drosophila melanogaster, GI78706872, Length=529, Percent_Identity=30.2457466918715, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24583543, Length=529, Percent_Identity=30.2457466918715, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI78706876, Length=529, Percent_Identity=30.2457466918715, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24660907, Length=593, Percent_Identity=26.1382799325464, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24660903, Length=593, Percent_Identity=26.1382799325464, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 65990; Mature: 65859

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLPETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTTPAAAAITSGGATTVSPYSKTAPLTA
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCEECCCCCCCCCEE
QLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGD
EEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHEECCC
EPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAVLSRDETLIALLADKPALQLYAKNTP
CCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHHCCCCEEEEEEECCCCEEEEECCCC
FVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEVHITVGVVRYDIDGRARSG
HHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCC
GASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQREVDGA
CCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCC
SGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAEL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHHHHH
WNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMGAEQADEYLSELRQARRYQRDVY
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTQAPPTSLLPLSPEQLARLQAAVGEFSPTQMAWLSGYFWGMVNQQPGAVASPAVAAPP
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCC
PVTVTLISASQTGNARRLAEQLRDDLLAAQLSVNLVNAGDYKFKQIAQERLLVVVASTQG
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHCCEEEEEEECCC
EGEPAEEAVALHKFLFSKKAPKLPETAFAVFGLGDTSYEHFCQAGKDFDSKLAELGAQRL
CCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHH
LDRVDADVEYQVQAQQWRQQVVATLQAKVPAQSTTPAAAAITSGGATTVSPYSKTAPLTA
HHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHEEECCCCCEECCCCCCCCCEE
QLSVQQKVTGRNSEKDVRHIEIDLGDSGLRYQPGDALGVWFDNDPALVEELLALLWLKGD
EEEHHHHHCCCCCCCCCEEEEEEECCCCCEECCCCEEEEEECCCHHHHHHHHHHHEECCC
EPVSIDGQNMPLAQALLSHLELTQNTTLIVDKYAVLSRDETLIALLADKPALQLYAKNTP
CCEEECCCCCCHHHHHHHHHHHCCCCEEEEECHHHHCCCCEEEEEEECCCCEEEEECCCC
FVDMVRQAPSDLNADQLVGLLRPLTPRLYSIASSQAETENEVHITVGVVRYDIDGRARSG
HHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCC
GASGYLADRLEVDGDIRVFIEHNDNFRLPANPETPVIMIGPGTGIAPFRAFMQQREVDGA
CCCCEEEHHEECCCCEEEEEEECCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCC
SGKNWLFFGNPHFTEDFLYQVEWQRYVKEGVLTRIDLAWSRDQAHKIYVQDKLREQGAEL
CCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHHHHH
WNWIQQGAHIYVCGDANRMAKDVEQVLLDVVALHGAMGAEQADEYLSELRQARRYQRDVY
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA