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The map label for this gene is cysI [H]

Identifier: 170025656

GI number: 170025656

Start: 3773197

End: 3774927

Strand: Reverse

Name: cysI [H]

Synonym: YPK_3441

Alternate gene names: 170025656

Gene position: 3774927-3773197 (Counterclockwise)

Preceding gene: 170025657

Following gene: 170025655

Centisome position: 80.5

GC content: 50.55

Gene sequence:

>1731_bases
ATGAATGAAAAACACCCAGGGCCACTCGTCGTCAGTGGAAAACTTTCTGATGGCGAGCGCATGAAGTCGGAAAGCAATTT
CTTGCGTGGGACTATTGCGGAAGATTTGAACAATGGACTAACTGGCGGTTTCAGCGGCGATAATTTTCTGTTGATCCGTT
TTCACGGCATGTACCAGCAGGATGACCGTGATATCCGCGCTGAACGTGCTGAGCAGAAGCTAGAGCCGCGCCATGCCATG
ATGCTGCGTTGCCGCTTGCCGGGTGGGATTATTACCCCGCAACAGTGGTTAGGTATTGACAAGTTTGCCGCTGATAACAC
CCTATATGGCAGTATTCGCATCACTAACCGCCAGACCTTTCAGTTCCATGGCATTTTGAAAGGCAATGTGAAACCCGCAC
ATCAATTGCTGAATGAGTTGGGATTAGATGCACTGGCGACGGCGAACGATGTAAACCGTAATGTGCTGTGTACTTCAAAT
CCGGTGGAATCGGCCTTGCATCAGGAAGCCTACGAATGGGCGAAGAAGATTTCGGAACATTTATTACCACGTACCCGCGC
ATATGCCGAAATCTGGCTGGATGCGGAAAAAGTCGCGACCACGGATGAAGAGCCGATCCTCGGTGCAACGTATTTACCTC
GTAAATTTAAAACGACAGTTGTTATTCCACCGCAGAATGACGTTGATTTGCATGCCAACGATTTGAACTTTGTTGCGGTG
GCAGATAAAGGCAAGTTGATTGGATTTAACGTGTTGGTGGGGGGGGGATTATCTATTGCCCATGGCGATAAGAATACCTA
TCCGCGCAAAGCCAGTGAATTTGGTTACATCCCGCTGAAACACACGTTGGCGATAGCGGAAGCGGTCGTGACGACCCAGC
GTGATTGGGGCAATCGCACGGATCGTAAAAATGCCAAGACCAAATATACGCTGGAGCGGGTGGGGGTAGAGACCTTCAAA
GCAGAAGTTGAGAAACGAGCTGGCGTTAGCTTTAGTGCGATCAAACCGTATCAATTTACCGGGCGCGGTGATCGTATCGG
CTGGGTGAAAGGTGTTGACAAAAAGTGGCATCTGACATTATTTATTGAGAATGGTCGTCTGTTGGATTACCCCGGTCGTA
GTCTGAAAACCGGAGTGGCTGAAATTGCTAAAATTCATCAGGGCGATTTCCGTCTGACGGCTAACCAGAACCTGATTGTG
GCAGGCGTTCCGGAAAAAGATAAAGCCCGGATTGAGGCACTGGCCCGTGAACATGGCTTGATGGATGACAATGTCACGTC
ACAGCGTGAAAACTCGATGGCGTGCGTCTCTTTCCCAACCTGTCCGCTGGCTATGGCAGAGGCTGAACGCTTCCTACCCG
AGTTTGTCACCCGCGTTGAGGGTATTTTGCAGCAACATGGCCTGGCGGATGAACATATTGTTCTGCGGGTGACCGGTTGC
CCAAATGGTTGTGGCCGCGCATTACTGGCCGAGGTGGGGTTGGTGGGCAAAGCCGTTGGCCGCTATAACCTGCATTTGGG
CGGTAACCGCGAAGGTACCCGTATTCCAAGGATGTACCGTGAGAATATTACCGCAGATGAAATTCTGCTTATCACTGACC
AACTGGTTGGGCGCTGGGCTAAAGAGCGTCATGTCGATGAAGGTTTTGGTGATTTTGTTATTCGTGCAGGGGTCATTGCA
CCAGTGATTGACTCTGCCCGTGATTTTTACGACGTACAGGAGGCGATGTGA

Upstream 100 bases:

>100_bases
TAGACGTGGTGGCTCTGCACGGTGCAATGGGTGCCGAGCAGGCTGATGAATATTTAAGTGAGTTGCGCCAAGCGCGCCGC
TATCAGCGAGATGTGTACTG

Downstream 100 bases:

>100_bases
GTCAATTTAATCTGAGCGAACTGAATGCGTTGCCGAAAGCCAAGCAAGCGGCAGCGCTGGTGCTGGTGAATGGTCAACTC
GAGCATTTGACCGCGCAGGA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP [H]

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MNEKHPGPLVVSGKLSDGERMKSESNFLRGTIAEDLNNGLTGGFSGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAM
MLRCRLPGGIITPQQWLGIDKFAADNTLYGSIRITNRQTFQFHGILKGNVKPAHQLLNELGLDALATANDVNRNVLCTSN
PVESALHQEAYEWAKKISEHLLPRTRAYAEIWLDAEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDLNFVAV
ADKGKLIGFNVLVGGGLSIAHGDKNTYPRKASEFGYIPLKHTLAIAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFK
AEVEKRAGVSFSAIKPYQFTGRGDRIGWVKGVDKKWHLTLFIENGRLLDYPGRSLKTGVAEIAKIHQGDFRLTANQNLIV
AGVPEKDKARIEALAREHGLMDDNVTSQRENSMACVSFPTCPLAMAEAERFLPEFVTRVEGILQQHGLADEHIVLRVTGC
PNGCGRALLAEVGLVGKAVGRYNLHLGGNREGTRIPRMYRENITADEILLITDQLVGRWAKERHVDEGFGDFVIRAGVIA
PVIDSARDFYDVQEAM

Sequences:

>Translated_576_residues
MNEKHPGPLVVSGKLSDGERMKSESNFLRGTIAEDLNNGLTGGFSGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAM
MLRCRLPGGIITPQQWLGIDKFAADNTLYGSIRITNRQTFQFHGILKGNVKPAHQLLNELGLDALATANDVNRNVLCTSN
PVESALHQEAYEWAKKISEHLLPRTRAYAEIWLDAEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDLNFVAV
ADKGKLIGFNVLVGGGLSIAHGDKNTYPRKASEFGYIPLKHTLAIAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFK
AEVEKRAGVSFSAIKPYQFTGRGDRIGWVKGVDKKWHLTLFIENGRLLDYPGRSLKTGVAEIAKIHQGDFRLTANQNLIV
AGVPEKDKARIEALAREHGLMDDNVTSQRENSMACVSFPTCPLAMAEAERFLPEFVTRVEGILQQHGLADEHIVLRVTGC
PNGCGRALLAEVGLVGKAVGRYNLHLGGNREGTRIPRMYRENITADEILLITDQLVGRWAKERHVDEGFGDFVIRAGVIA
PVIDSARDFYDVQEAM
>Mature_576_residues
MNEKHPGPLVVSGKLSDGERMKSESNFLRGTIAEDLNNGLTGGFSGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAM
MLRCRLPGGIITPQQWLGIDKFAADNTLYGSIRITNRQTFQFHGILKGNVKPAHQLLNELGLDALATANDVNRNVLCTSN
PVESALHQEAYEWAKKISEHLLPRTRAYAEIWLDAEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDLNFVAV
ADKGKLIGFNVLVGGGLSIAHGDKNTYPRKASEFGYIPLKHTLAIAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFK
AEVEKRAGVSFSAIKPYQFTGRGDRIGWVKGVDKKWHLTLFIENGRLLDYPGRSLKTGVAEIAKIHQGDFRLTANQNLIV
AGVPEKDKARIEALAREHGLMDDNVTSQRENSMACVSFPTCPLAMAEAERFLPEFVTRVEGILQQHGLADEHIVLRVTGC
PNGCGRALLAEVGLVGKAVGRYNLHLGGNREGTRIPRMYRENITADEILLITDQLVGRWAKERHVDEGFGDFVIRAGVIA
PVIDSARDFYDVQEAM

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate [H]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=571, Percent_Identity=80.7355516637478, Blast_Score=995, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=590, Percent_Identity=42.0338983050847, Blast_Score=486, Evalue=1e-138,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 64079; Mature: 64079

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKHPGPLVVSGKLSDGERMKSESNFLRGTIAEDLNNGLTGGFSGDNFLLIRFHGMYQQ
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
DDRDIRAERAEQKLEPRHAMMLRCRLPGGIITPQQWLGIDKFAADNTLYGSIRITNRQTF
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCHHHCCCCEEEEEEEECCCEEE
QFHGILKGNVKPAHQLLNELGLDALATANDVNRNVLCTSNPVESALHQEAYEWAKKISEH
EEEEEEECCCCHHHHHHHHHCCHHEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LLPRTRAYAEIWLDAEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDLNFVAV
HCCHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEEE
ADKGKLIGFNVLVGGGLSIAHGDKNTYPRKASEFGYIPLKHTLAIAEAVVTTQRDWGNRT
ECCCCEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
DRKNAKTKYTLERVGVETFKAEVEKRAGVSFSAIKPYQFTGRGDRIGWVKGVDKKWHLTL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCEEEEEECCCCEEEEEE
FIENGRLLDYPGRSLKTGVAEIAKIHQGDFRLTANQNLIVAGVPEKDKARIEALAREHGL
EEECCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
MDDNVTSQRENSMACVSFPTCPLAMAEAERFLPEFVTRVEGILQQHGLADEHIVLRVTGC
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PNGCGRALLAEVGLVGKAVGRYNLHLGGNREGTRIPRMYRENITADEILLITDQLVGRWA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEHHHHHHHH
KERHVDEGFGDFVIRAGVIAPVIDSARDFYDVQEAM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEKHPGPLVVSGKLSDGERMKSESNFLRGTIAEDLNNGLTGGFSGDNFLLIRFHGMYQQ
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCC
DDRDIRAERAEQKLEPRHAMMLRCRLPGGIITPQQWLGIDKFAADNTLYGSIRITNRQTF
CCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHCCCHHHCCCCEEEEEEEECCCEEE
QFHGILKGNVKPAHQLLNELGLDALATANDVNRNVLCTSNPVESALHQEAYEWAKKISEH
EEEEEEECCCCHHHHHHHHHCCHHEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
LLPRTRAYAEIWLDAEKVATTDEEPILGATYLPRKFKTTVVIPPQNDVDLHANDLNFVAV
HCCHHHHHHHHEECHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCCEEEEE
ADKGKLIGFNVLVGGGLSIAHGDKNTYPRKASEFGYIPLKHTLAIAEAVVTTQRDWGNRT
ECCCCEEEEEEEEECCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
DRKNAKTKYTLERVGVETFKAEVEKRAGVSFSAIKPYQFTGRGDRIGWVKGVDKKWHLTL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCCCEEEEEECCCCEEEEEE
FIENGRLLDYPGRSLKTGVAEIAKIHQGDFRLTANQNLIVAGVPEKDKARIEALAREHGL
EEECCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
MDDNVTSQRENSMACVSFPTCPLAMAEAERFLPEFVTRVEGILQQHGLADEHIVLRVTGC
CCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
PNGCGRALLAEVGLVGKAVGRYNLHLGGNREGTRIPRMYRENITADEILLITDQLVGRWA
CCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHHHCCCCCCEEEEEHHHHHHHH
KERHVDEGFGDFVIRAGVIAPVIDSARDFYDVQEAM
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA