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The map label for this gene is bioD [H]

Identifier: 170025150

GI number: 170025150

Start: 3226620

End: 3227357

Strand: Reverse

Name: bioD [H]

Synonym: YPK_2928

Alternate gene names: 170025150

Gene position: 3227357-3226620 (Counterclockwise)

Preceding gene: 170025151

Following gene: 170025148

Centisome position: 68.82

GC content: 53.79

Gene sequence:

>738_bases
ATGGGGTTATCTATCGTGACTAAACGTTGGTTTATCACCGGTACCGATACTGATGTCGGTAAAACCGTCGCCAGTTGTGC
ATTACTGCAAGCGGCCACCGCTCAGGGGTACCATACTGCTGGCTATAAACCGGTAGCATCCGGTAGCCAGATGACCGCTG
ACGGCTTGCGCAATAGTGATGCCCTGGCGTTGCAGGCGAATAGCAGCCAGAGGCTCGGTTACTCACAAGTGAATCCGTTC
ACTTTTTTGGAAGCGACATCACCGCATATTGCCAGTGAAAGTGAAGGGAGGGCGATCCCTTTGACGGCGTTATCACAGGG
TTTACGTCAACTGGAACCCTCCGCTGACTGGATATTGATAGAAGGGGCCGGCGGCTGGTTTACCCCGCTTTCTCCGCAGG
CCACATTTGCCGATTGGGTACAACAGGAACAATTGCCGGTCATTATGGTGGTTGGGGTGAAACTGGGGTGTATCAACCAT
GCGTTGCTCACGGCACAGGCGATTCAGCACGCCGGGCTGACACTGGCCGGTTGGGTTGCCAATGAGGTGACACCGGCAGG
GAGACGGCAGGCCGAATATCAGGCCACATTGACCCGGATGATAACCGCGCCATTGCTGGGAATTATTCCCTATCTTTCTG
ATATAGAAGAAAATCCGGTGACAACACGCCGTGATCTTGGGCACTACTTGGATTTGACGGTATTACGTGCAGCAGAACGG
GAGGCTGTCAACATGTGA

Upstream 100 bases:

>100_bases
GGGCGTCAGGCTGGGTTACAGGGGCGTCAACGTCTGGCGGCGCTACAACGGGCCTATGTCATGCAATCTGGCGGCTATCC
ACTGAGTTATCATATGGTTT

Downstream 100 bases:

>100_bases
TACATATATGGACGCCGCTACAAGCATGTGATGTGGCTTGCAATCAGAGGCCATCAGGTGCGATTAACCTTATGTAGTTA
ATCTACCCGAGTATAGTTAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYHTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPF
TFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINH
ALLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
EAVNM

Sequences:

>Translated_245_residues
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYHTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPF
TFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINH
ALLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
EAVNM
>Mature_244_residues
GLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYHTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPFT
FLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHA
LLTAQAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAERE
AVNM

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=230, Percent_Identity=70.4347826086957, Blast_Score=327, Evalue=4e-91,
Organism=Escherichia coli, GI87081944, Length=230, Percent_Identity=49.5652173913044, Blast_Score=209, Evalue=9e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 26271; Mature: 26140

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYHTAGYKPVASGSQMTADGLRNSD
CCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALALQANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILI
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEE
EGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVA
ECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHH
NEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAVNM
HHCCC
>Mature Secondary Structure 
GLSIVTKRWFITGTDTDVGKTVASCALLQAATAQGYHTAGYKPVASGSQMTADGLRNSD
CCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
ALALQANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILI
EEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEE
EGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVA
ECCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHH
NEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAER
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EAVNM
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]