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The map label for this gene is uvrB

Identifier: 170025148

GI number: 170025148

Start: 3222614

End: 3224629

Strand: Reverse

Name: uvrB

Synonym: YPK_2926

Alternate gene names: 170025148

Gene position: 3224629-3222614 (Counterclockwise)

Preceding gene: 170025150

Following gene: 170025147

Centisome position: 68.76

GC content: 49.85

Gene sequence:

>2016_bases
ATGAGCAAATCATTCAAACTGCATTCTGTGTTTAAGCCAGCTGGCGATCAGCCTGAGGCTATCCGTAAATTGGAGGAGGG
GCTGGAAAATGGTCTGGCACACCAGACCCTGCTAGGCGTAACAGGATCAGGCAAAACCTTTACGGTTGCTAATGTTATTG
CCGATCTGAACCGGCCAACCATGATCCTGGCCCCGAATAAAACACTGGCAGCGCAACTTTACGGCGAGATGAAAGAGTTC
TTCCCGGATAACGCGGTAGAGTACTTTGTCTCCTACTACGATTATTATCAACCTGAAGCTTACGTGCCCAGTTCTGATAC
TTTTATTGAGAAGGATGCGTCGGTCAATGAACACATCGAGCAGATGCGGCTATCCGCCACCAAGGCCTTGTTAGAGCGGC
GAGATGTGGTGGTCGTGGCGTCGGTTTCCGCCATTTATGGTCTGGGCGATCCAGATCTGTATCTGAAAATGATGTTGCAC
CTAACCCGTGGCATGATCATTGATCAACGTTCCATTTTACGTCGCCTGTCAGAATTGCAATACAGCCGCAACGATCAGGT
ATTTCAACGCGGTACTTTTCGGGTTCGGGGTGAAGTGATCGATATCTTCCCGGCAGAATCTGATGAGTGGGCGCTGCGGG
TAGAATTATTTGATGAGGAAGTCGAGCGATTATCCATTTTCGATCCACTCACGGGTCAGCTACAGCATGAAGTCCCTCGC
TTTACTGTTTATCCTAAAACACATTACGTCACCCCACGGGAACGCATTCTACAGGCGATGGAAGAGATAAAAGTCGAGCT
GGCGGAGCGGCGTCAGGTGTTGTTAGCCAATAACAAATTATTGGAAGAGCAACGTCTCTCCCAACGTACTCAGTTTGATC
TTGAAATGATGAATGAGCTGGGCTATTGCTCGGGTATCGAAAACTATTCTCGCTATCTCTCTGGCCGTGGGCCGGGTGAA
GCGCCTCCCACGTTATTTGACTATTTACCGGCTGATGGTTTGTTGATTGTCGATGAGTCCCACGTCACTATTCCGCAAAT
TGGTGGCATGTACAAAGGCGACCGCTCACGTAAAGAAACCCTGGTAGAGTACGGTTTCCGTTTGCCATCGGCACTGGATA
ACCGGCCAATGCGCTTTGAAGAGTTCGAAGCGTTGGCACCGCAAACGATCTATGTTTCGGCCACACCGGGCAAATACGAG
CTGGAGAAATCTGGCGGTGACATTATTGAGCAAGTGGTGCGGCCAACGGGCCTACTCGATCCGTTGATCGAGGTCCGCCC
GGTAGCCACACAAGTGGATGACTTGCTGTCAGAGATCCGCATCCGTGCTGCGATTAACGAGCGGGTACTGGTGACCACGC
TAACGAAACGAATGGCTGAGGACTTAACCGACTATCTCAGCGAGCATGGCGCCAAAGTACGTTACCTGCATTCAGATATC
GATACTGTGGAGCGGGTGGAGATTATTCGCGATTTACGCTTGGGTGAATTTGATGTGCTGGTGGGCATCAACTTGCTGCG
GGAAGGGTTGGATATGCCAGAGGTCTCATTGGTTGCCATTCTTGATGCAGATAAAGAGGGCTTCTTGCGTTCTGAACGCT
CCTTGATTCAGACTATTGGTCGTGCTGCCCGTAACCTCAACGGCAAGGCGATTTTATACGGCGATAGAATTACCGCCTCG
ATGGAAAAAGCCATTGGCGAAACGGAACGGCGGCGCGCTAAACAGCAGGCGTATAATGAAGAACGCGGGATTATTCCTCA
GGGGCTAAACAAAAAGATTGGCGATATCCTGCAATTGGGCCAGCCTTCAATGCGCGGCAAAGGAAAAGGGCGCGGCAGTC
ATAAAATGGCCGATACCACCCAATATCAATCTTTGTCGCCGAAAGCACTTGATCAGAAAATCCGTGAACTGGAAGCAAAA
ATGTATACATATGCGCAAAACCTGGAGTTTGAACAGGCGGCTGAATTACGTGATCAGGTTCATCAACTGCGGCAACAGTT
TATTGCAATATCTTAA

Upstream 100 bases:

>100_bases
GAAGCTGGTTTTATATCCAGTATCATAAGGTTGGCAAAAATCTCGGGGGCTTTCATAATGAGTCCCCTGACAGTGTGTTT
CTCCCGCGGGTAGCGAATTC

Downstream 100 bases:

>100_bases
TTTTTATTGCGACATCTTAAGTTTATCTCGCTGCTTTAAGGTTTGCTTCAGCGCCTTAAGGTTTATCGAGATGCCTTAAA
ACTTATTTCAATACCTTAAG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MSKSFKLHSVFKPAGDQPEAIRKLEEGLENGLAHQTLLGVTGSGKTFTVANVIADLNRPTMILAPNKTLAAQLYGEMKEF
FPDNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIEQMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLH
LTRGMIIDQRSILRRLSELQYSRNDQVFQRGTFRVRGEVIDIFPAESDEWALRVELFDEEVERLSIFDPLTGQLQHEVPR
FTVYPKTHYVTPRERILQAMEEIKVELAERRQVLLANNKLLEEQRLSQRTQFDLEMMNELGYCSGIENYSRYLSGRGPGE
APPTLFDYLPADGLLIVDESHVTIPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPMRFEEFEALAPQTIYVSATPGKYE
LEKSGGDIIEQVVRPTGLLDPLIEVRPVATQVDDLLSEIRIRAAINERVLVTTLTKRMAEDLTDYLSEHGAKVRYLHSDI
DTVERVEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITAS
MEKAIGETERRRAKQQAYNEERGIIPQGLNKKIGDILQLGQPSMRGKGKGRGSHKMADTTQYQSLSPKALDQKIRELEAK
MYTYAQNLEFEQAAELRDQVHQLRQQFIAIS

Sequences:

>Translated_671_residues
MSKSFKLHSVFKPAGDQPEAIRKLEEGLENGLAHQTLLGVTGSGKTFTVANVIADLNRPTMILAPNKTLAAQLYGEMKEF
FPDNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIEQMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLH
LTRGMIIDQRSILRRLSELQYSRNDQVFQRGTFRVRGEVIDIFPAESDEWALRVELFDEEVERLSIFDPLTGQLQHEVPR
FTVYPKTHYVTPRERILQAMEEIKVELAERRQVLLANNKLLEEQRLSQRTQFDLEMMNELGYCSGIENYSRYLSGRGPGE
APPTLFDYLPADGLLIVDESHVTIPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPMRFEEFEALAPQTIYVSATPGKYE
LEKSGGDIIEQVVRPTGLLDPLIEVRPVATQVDDLLSEIRIRAAINERVLVTTLTKRMAEDLTDYLSEHGAKVRYLHSDI
DTVERVEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITAS
MEKAIGETERRRAKQQAYNEERGIIPQGLNKKIGDILQLGQPSMRGKGKGRGSHKMADTTQYQSLSPKALDQKIRELEAK
MYTYAQNLEFEQAAELRDQVHQLRQQFIAIS
>Mature_670_residues
SKSFKLHSVFKPAGDQPEAIRKLEEGLENGLAHQTLLGVTGSGKTFTVANVIADLNRPTMILAPNKTLAAQLYGEMKEFF
PDNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIEQMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLHL
TRGMIIDQRSILRRLSELQYSRNDQVFQRGTFRVRGEVIDIFPAESDEWALRVELFDEEVERLSIFDPLTGQLQHEVPRF
TVYPKTHYVTPRERILQAMEEIKVELAERRQVLLANNKLLEEQRLSQRTQFDLEMMNELGYCSGIENYSRYLSGRGPGEA
PPTLFDYLPADGLLIVDESHVTIPQIGGMYKGDRSRKETLVEYGFRLPSALDNRPMRFEEFEALAPQTIYVSATPGKYEL
EKSGGDIIEQVVRPTGLLDPLIEVRPVATQVDDLLSEIRIRAAINERVLVTTLTKRMAEDLTDYLSEHGAKVRYLHSDID
TVERVEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITASM
EKAIGETERRRAKQQAYNEERGIIPQGLNKKIGDILQLGQPSMRGKGKGRGSHKMADTTQYQSLSPKALDQKIRELEAKM
YTYAQNLEFEQAAELRDQVHQLRQQFIAIS

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=673, Percent_Identity=85.8841010401189, Blast_Score=1166, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=307, Percent_Identity=23.7785016286645, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_YERP3 (A7FKM4)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001401802.1
- ProteinModelPortal:   A7FKM4
- STRING:   A7FKM4
- GeneID:   5387735
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_2839
- eggNOG:   COG0556
- HOGENOM:   HBG703949
- OMA:   AAQLCNE
- ProtClustDB:   PRK05298
- BioCyc:   YPSE349747:YPSIP31758_2839-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 76247; Mature: 76116

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSFKLHSVFKPAGDQPEAIRKLEEGLENGLAHQTLLGVTGSGKTFTVANVIADLNRPT
CCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCCCCEEEHHHHHHHCCCCE
MILAPNKTLAAQLYGEMKEFFPDNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIE
EEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLHLTRGMIIDQRSILRRLSELQ
HHHHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHH
YSRNDQVFQRGTFRVRGEVIDIFPAESDEWALRVELFDEEVERLSIFDPLTGQLQHEVPR
HCCCCHHHHCCCEEEECEEEEEECCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHCCCC
FTVYPKTHYVTPRERILQAMEEIKVELAERRQVLLANNKLLEEQRLSQRTQFDLEMMNEL
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GYCSGIENYSRYLSGRGPGEAPPTLFDYLPADGLLIVDESHVTIPQIGGMYKGDRSRKET
CCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHH
LVEYGFRLPSALDNRPMRFEEFEALAPQTIYVSATPGKYELEKSGGDIIEQVVRPTGLLD
HHHHHCCCCHHHCCCCCCHHHHHHCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCHHH
PLIEVRPVATQVDDLLSEIRIRAAINERVLVTTLTKRMAEDLTDYLSEHGAKVRYLHSDI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
DTVERVEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIG
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
RAARNLNGKAILYGDRITASMEKAIGETERRRAKQQAYNEERGIIPQGLNKKIGDILQLG
HHHHCCCCCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
QPSMRGKGKGRGSHKMADTTQYQSLSPKALDQKIRELEAKMYTYAQNLEFEQAAELRDQV
CCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HQLRQQFIAIS
HHHHHHHHCCC
>Mature Secondary Structure 
SKSFKLHSVFKPAGDQPEAIRKLEEGLENGLAHQTLLGVTGSGKTFTVANVIADLNRPT
CCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHEEECCCCCEEEHHHHHHHCCCCE
MILAPNKTLAAQLYGEMKEFFPDNAVEYFVSYYDYYQPEAYVPSSDTFIEKDASVNEHIE
EEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
QMRLSATKALLERRDVVVVASVSAIYGLGDPDLYLKMMLHLTRGMIIDQRSILRRLSELQ
HHHHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHHHHHHHHHHHCCEECHHHHHHHHHHHH
YSRNDQVFQRGTFRVRGEVIDIFPAESDEWALRVELFDEEVERLSIFDPLTGQLQHEVPR
HCCCCHHHHCCCEEEECEEEEEECCCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHCCCC
FTVYPKTHYVTPRERILQAMEEIKVELAERRQVLLANNKLLEEQRLSQRTQFDLEMMNEL
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHC
GYCSGIENYSRYLSGRGPGEAPPTLFDYLPADGLLIVDESHVTIPQIGGMYKGDRSRKET
CCCCCHHHHHHHHCCCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHH
LVEYGFRLPSALDNRPMRFEEFEALAPQTIYVSATPGKYELEKSGGDIIEQVVRPTGLLD
HHHHHCCCCHHHCCCCCCHHHHHHCCCCEEEEEECCCCEEECCCCHHHHHHHHCCCCHHH
PLIEVRPVATQVDDLLSEIRIRAAINERVLVTTLTKRMAEDLTDYLSEHGAKVRYLHSDI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHH
DTVERVEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIG
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHH
RAARNLNGKAILYGDRITASMEKAIGETERRRAKQQAYNEERGIIPQGLNKKIGDILQLG
HHHHCCCCCEEEECCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
QPSMRGKGKGRGSHKMADTTQYQSLSPKALDQKIRELEAKMYTYAQNLEFEQAAELRDQV
CCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
HQLRQQFIAIS
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA