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The map label for this gene is gadB [C]

Identifier: 169630819

GI number: 169630819

Start: 3793144

End: 3794574

Strand: Reverse

Name: gadB [C]

Synonym: MAB_3740c

Alternate gene names: 169630819

Gene position: 3794574-3793144 (Counterclockwise)

Preceding gene: 169630820

Following gene: 169630818

Centisome position: 74.89

GC content: 63.1

Gene sequence:

>1431_bases
ATGTCGAAATCCCGTGATCAGTTCCGTCTCTCGGCGCATGCCGGAAAGATCAATGCCTCCGCCTTCAGCCCCGCGTACAC
CGGCAGGCTGTCCACGGCACCCGTCCCGGCGCTGCGACTGCCCGACGAGCCGATGGATCCGCAGGCCGCCTATCGGTTCA
TTCACGACGAGCTGATGCTCGACGGCAGCTCGCGGCTGAACTTGGCGACCTTCGTCACGACGTGGATGGATCCCGAGGCC
GAAAAGCTGATGGCCGAGACGTTCGACAAGAACATGATCGACAAGGACGAGTATCCGGCGACGGCCGCGATCGAGTCGCG
ATGTGTGGCCATGGTGGCCGACCTGTTCCATGCCGACAACTTGTCCACGGAGGATCCGTCCTCGGCGGTAGGGGTGTCGA
CCATCGGATCGAGTGAGGCGGTCATGCTGGCCGGGCTGGCCCTCAAGTGGCGTTGGCGCACGAAAATCGGCGGTACCGAC
GGCACCGGCTGGAAGGGGCGCACACCCAACCTGGTGATGGGTTCCAACGTGCAGGTGGTCTGGGAAAAATTCTGCCGGTA
CTTCGACGTCGAACCGCGATACCTGCCGATGGCCAAGGGCCGGTACGTGATCACTCCCGAGCAGGTGCTTGAGGTGGTCG
ACGAGGACACCATCGGCGTCGTCGGGATCCTGGGCACCACGTTCACCGGAGAGCTGGAGCCGATCGCCGCGATCTGCGCG
GCGCTGGACAAGCTCGCTGCTACACCCGGCAAGCCCGATGTGCCGGTACACGTCGATGCCGCCAGCGGTGGTTTCGTGGT
GCCTTTCCTGCATCCCGAACTGCACTGGGACTTCCGGTTGCCGCGGGTGGTGTCGATCAATGTCAGCGGACACAAGTACG
GGCTGACCTATCCCGGGATCGGCTTTGTGGTGTGGCGCGGCAAGGAGCATCTGCCCGAAGACCTGGTGTTCCGGGTCAAC
TACCTGGGTGGCGACATGCCGACCTTCACCCTGAACTTTTCCCGCCCGGGCAACCAGGTGGTGGGGCAGTACTACAACTT
CTTGCGGCTGGGCCGGGCCGGATATGCACAGGTGATGCGCTGTCTGTCGGAGACGGCACGCTGGTTCGGCGACGAACTCG
GCAAGAGTGAACATTTCGAGGTGATCACCGACGGGTCGGCGATCCCGGTGGTGTCGTTCCGGCTCAAAGGCAAGCGGCCG
TACACCGAGTTCGACATCTCGCATTCGTTGCGGGCATTTGGCTGGCAGGTGCCTGCGTACACCATGCCCGATGACGTCAC
CGATATCGCCGTACTGCGAGTGGTTGTGCGCGAGGGCTTCTCGGGAGATCTGGCCAGAGCGCTGCGCGATGATCTCAACA
CCGTGCTCAAGGGACTCGACGAGCTGAAACCGAACGGACACTTCGACGAGGTGCAGCCGTTCGCTCATTAG

Upstream 100 bases:

>100_bases
GCTGCCGCCGACCCGGGTCCGGGTAAAGCGCCCATTTCGGCTTCTCGTCTTCTTGGACATCTGCGGTGGGCGATCGCCGA
AATAGGGTGAGAATCGGTTT

Downstream 100 bases:

>100_bases
CGTGCGGATGGGACAATGGGGCCGGTGAGCACGACTTCGACGACAACCGACGGCCTTCCGCCGAGCACCGGTGCGACGAC
CGCGCAAGCGGTCATCGACC

Product: glutamate decarboxylase GadB

Products: NA

Alternate protein names: GAD-gamma [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKSRDQFRLSAHAGKINASAFSPAYTGRLSTAPVPALRLPDEPMDPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEA
EKLMAETFDKNMIDKDEYPATAAIESRCVAMVADLFHADNLSTEDPSSAVGVSTIGSSEAVMLAGLALKWRWRTKIGGTD
GTGWKGRTPNLVMGSNVQVVWEKFCRYFDVEPRYLPMAKGRYVITPEQVLEVVDEDTIGVVGILGTTFTGELEPIAAICA
ALDKLAATPGKPDVPVHVDAASGGFVVPFLHPELHWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRGKEHLPEDLVFRVN
YLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRAGYAQVMRCLSETARWFGDELGKSEHFEVITDGSAIPVVSFRLKGKRP
YTEFDISHSLRAFGWQVPAYTMPDDVTDIAVLRVVVREGFSGDLARALRDDLNTVLKGLDELKPNGHFDEVQPFAH

Sequences:

>Translated_476_residues
MSKSRDQFRLSAHAGKINASAFSPAYTGRLSTAPVPALRLPDEPMDPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEA
EKLMAETFDKNMIDKDEYPATAAIESRCVAMVADLFHADNLSTEDPSSAVGVSTIGSSEAVMLAGLALKWRWRTKIGGTD
GTGWKGRTPNLVMGSNVQVVWEKFCRYFDVEPRYLPMAKGRYVITPEQVLEVVDEDTIGVVGILGTTFTGELEPIAAICA
ALDKLAATPGKPDVPVHVDAASGGFVVPFLHPELHWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRGKEHLPEDLVFRVN
YLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRAGYAQVMRCLSETARWFGDELGKSEHFEVITDGSAIPVVSFRLKGKRP
YTEFDISHSLRAFGWQVPAYTMPDDVTDIAVLRVVVREGFSGDLARALRDDLNTVLKGLDELKPNGHFDEVQPFAH
>Mature_475_residues
SKSRDQFRLSAHAGKINASAFSPAYTGRLSTAPVPALRLPDEPMDPQAAYRFIHDELMLDGSSRLNLATFVTTWMDPEAE
KLMAETFDKNMIDKDEYPATAAIESRCVAMVADLFHADNLSTEDPSSAVGVSTIGSSEAVMLAGLALKWRWRTKIGGTDG
TGWKGRTPNLVMGSNVQVVWEKFCRYFDVEPRYLPMAKGRYVITPEQVLEVVDEDTIGVVGILGTTFTGELEPIAAICAA
LDKLAATPGKPDVPVHVDAASGGFVVPFLHPELHWDFRLPRVVSINVSGHKYGLTYPGIGFVVWRGKEHLPEDLVFRVNY
LGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRAGYAQVMRCLSETARWFGDELGKSEHFEVITDGSAIPVVSFRLKGKRPY
TEFDISHSLRAFGWQVPAYTMPDDVTDIAVLRVVVREGFSGDLARALRDDLNTVLKGLDELKPNGHFDEVQPFAH

Specific function: Catalyzes The Production Of Gaba. [C]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=374, Percent_Identity=23.5294117647059, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1787769, Length=428, Percent_Identity=49.2990654205608, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1789934, Length=428, Percent_Identity=49.2990654205608, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17557272, Length=296, Percent_Identity=25.6756756756757, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17543922, Length=360, Percent_Identity=24.4444444444444, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25148342, Length=348, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323905, Length=499, Percent_Identity=39.6793587174349, Blast_Score=320, Evalue=4e-88,
Organism=Saccharomyces cerevisiae, GI6320500, Length=369, Percent_Identity=26.0162601626016, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21355963, Length=358, Percent_Identity=25.9776536312849, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24654344, Length=358, Percent_Identity=25.9776536312849, Blast_Score=112, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52637; Mature: 52506

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSRDQFRLSAHAGKINASAFSPAYTGRLSTAPVPALRLPDEPMDPQAAYRFIHDELML
CCCCCCCEEEEECCCEEECCCCCCHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHEE
DGSSRLNLATFVTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIESRCVAMVADLFHADN
CCCCCCEEEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LSTEDPSSAVGVSTIGSSEAVMLAGLALKWRWRTKIGGTDGTGWKGRTPNLVMGSNVQVV
CCCCCCCCCCCEEECCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEECCCHHHH
WEKFCRYFDVEPRYLPMAKGRYVITPEQVLEVVDEDTIGVVGILGTTFTGELEPIAAICA
HHHHHHHCCCCCCEEECCCCCEEECHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ALDKLAATPGKPDVPVHVDAASGGFVVPFLHPELHWDFRLPRVVSINVSGHKYGLTYPGI
HHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEECCCCCEEEEEEECCCEECCCCCCC
GFVVWRGKEHLPEDLVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRAGYAQVMR
EEEEECCCCCCCHHHEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
CLSETARWFGDELGKSEHFEVITDGSAIPVVSFRLKGKRPYTEFDISHSLRAFGWQVPAY
HHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCEECHHHHHHHHCEECCCC
TMPDDVTDIAVLRVVVREGFSGDLARALRDDLNTVLKGLDELKPNGHFDEVQPFAH
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKSRDQFRLSAHAGKINASAFSPAYTGRLSTAPVPALRLPDEPMDPQAAYRFIHDELML
CCCCCCEEEEECCCEEECCCCCCHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHEE
DGSSRLNLATFVTTWMDPEAEKLMAETFDKNMIDKDEYPATAAIESRCVAMVADLFHADN
CCCCCCEEEEEEEHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
LSTEDPSSAVGVSTIGSSEAVMLAGLALKWRWRTKIGGTDGTGWKGRTPNLVMGSNVQVV
CCCCCCCCCCCEEECCCCCEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEECCCHHHH
WEKFCRYFDVEPRYLPMAKGRYVITPEQVLEVVDEDTIGVVGILGTTFTGELEPIAAICA
HHHHHHHCCCCCCEEECCCCCEEECHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ALDKLAATPGKPDVPVHVDAASGGFVVPFLHPELHWDFRLPRVVSINVSGHKYGLTYPGI
HHHHHHCCCCCCCCCEEEECCCCCEEEEEECCCEEECCCCCEEEEEEECCCEECCCCCCC
GFVVWRGKEHLPEDLVFRVNYLGGDMPTFTLNFSRPGNQVVGQYYNFLRLGRAGYAQVMR
EEEEECCCCCCCHHHEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
CLSETARWFGDELGKSEHFEVITDGSAIPVVSFRLKGKRPYTEFDISHSLRAFGWQVPAY
HHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCCCEECHHHHHHHHCEECCCC
TMPDDVTDIAVLRVVVREGFSGDLARALRDDLNTVLKGLDELKPNGHFDEVQPFAH
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]