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The map label for this gene is prfB

Identifier: 169630559

GI number: 169630559

Start: 3518196

End: 3519299

Strand: Reverse

Name: prfB

Synonym: MAB_3478c

Alternate gene names: 169630559

Gene position: 3519299-3518196 (Counterclockwise)

Preceding gene: 169630560

Following gene: 169630558

Centisome position: 69.45

GC content: 66.21

Gene sequence:

>1104_bases
GTGGATCTGGACTGCCAAGCCGACATCGCTGACCTCGACACGACCCTCACCACGGTGGAGCGGGTGCTCGACGTCGACGG
CCTGCGTGCCCGGATCAAGACGCTGGAGGAGGCCGCCGCCGACCCCAACCTGTGGGACGACCAGGCCCGCGGCCAGCAGG
TGACGAGTCAGCTCTCCCACGCGCAGGGTGAGCTGCGCCGGGTCGAGGAGCTGCGGAGTCGCCTCGACGATCTGCCGGTG
CTTTACGAGCTTGCCGAAGAAAGCGAAGACAACTCGGTGGTGGCCGAGGCGGATGCCGAACGCGTCAAGCTGCGCGAGGA
CATCGAAGCCATGGAGGTCCGGACGCTGCTGTCCGGGGAGTACGACGAGCGCGAGGCCGTGGTCACCATCCGCTCGGGCG
CCGGCGGTGTCGACGCCGCCGACTGGGCCGAAATGCTCATGCGGATGTACATCCGCTGGGCCGAGGCGCACAAGTACCCG
GTCGAGGTCTTCGACACCTCCTATGCCGAAGAGGCGGGCATCAAGAGCGCGACCTTCGCCGTGCACGCTCCCTTTGCCTA
CGGCACACTGTCGGTGGAGCAGGGCACGCACCGCCTGGTACGCATCAGCCCGTTCGACAACCAAAGCCGGCGGCAGACCT
CGTTTGCCGATGTCGAGGTGCTTCCCGTGGTGGAGACGACCGACCACATCGACATTCCCGAAGGCGATCTGCGCGTTGAC
GTCTATCGATCGAGTGGGCCGGGCGGCCAGTCGGTGAACACCACCGACTCGGCGGTTCGTCTTACGCACATCCCGACCGG
CATCGTCGTCACCTGCCAGAACGAGAAGTCGCAGTTGCAGAACAAGGTGGCCGCGATGCGCGTGCTGCAGGCCCGGCTGC
TGGAACGCAAGCGGCAGGAGGAGCGCGCCGAGATGGATGCGCTGAAGGGGGACGGCGGTAGCTCATGGGGCACGCAGATG
CGCTCCTATGTGTTGCAGCCGTACCAGATGGTCAAGGACCTTCGCACCGAGTACGAGGTGGGTAATCCCGCGGCGGTGCT
CGACGGGGACATCGACGGATTCATCGAGGCGGGGATCCGGTGGCGCAACAGGCGCGATGATTAG

Upstream 100 bases:

>100_bases
GCCGATCACATCACCTCGATCACCCATGGCAGGCAGGGCTCCGGCACATAGCCGTCAGGTGGACATGGGACCGGGCGTAT
GACCGGTAACCTGAACTGTT

Downstream 100 bases:

>100_bases
AGCGGCCGAATTCAGCGCGGGCGGGCTGCGGCTGGCCGAACGCTGGAACTCCTTCTGGTCCAGCCAGATTGGTCGTTGGC
TGCTCGATAACGGTTTGCAG

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MDLDCQADIADLDTTLTTVERVLDVDGLRARIKTLEEAAADPNLWDDQARGQQVTSQLSHAQGELRRVEELRSRLDDLPV
LYELAEESEDNSVVAEADAERVKLREDIEAMEVRTLLSGEYDEREAVVTIRSGAGGVDAADWAEMLMRMYIRWAEAHKYP
VEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFADVEVLPVVETTDHIDIPEGDLRVD
VYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKVAAMRVLQARLLERKRQEERAEMDALKGDGGSSWGTQM
RSYVLQPYQMVKDLRTEYEVGNPAAVLDGDIDGFIEAGIRWRNRRDD

Sequences:

>Translated_367_residues
MDLDCQADIADLDTTLTTVERVLDVDGLRARIKTLEEAAADPNLWDDQARGQQVTSQLSHAQGELRRVEELRSRLDDLPV
LYELAEESEDNSVVAEADAERVKLREDIEAMEVRTLLSGEYDEREAVVTIRSGAGGVDAADWAEMLMRMYIRWAEAHKYP
VEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFADVEVLPVVETTDHIDIPEGDLRVD
VYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKVAAMRVLQARLLERKRQEERAEMDALKGDGGSSWGTQM
RSYVLQPYQMVKDLRTEYEVGNPAAVLDGDIDGFIEAGIRWRNRRDD
>Mature_367_residues
MDLDCQADIADLDTTLTTVERVLDVDGLRARIKTLEEAAADPNLWDDQARGQQVTSQLSHAQGELRRVEELRSRLDDLPV
LYELAEESEDNSVVAEADAERVKLREDIEAMEVRTLLSGEYDEREAVVTIRSGAGGVDAADWAEMLMRMYIRWAEAHKYP
VEVFDTSYAEEAGIKSATFAVHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFADVEVLPVVETTDHIDIPEGDLRVD
VYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKVAAMRVLQARLLERKRQEERAEMDALKGDGGSSWGTQM
RSYVLQPYQMVKDLRTEYEVGNPAAVLDGDIDGFIEAGIRWRNRRDD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=303, Percent_Identity=32.013201320132, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI34577120, Length=240, Percent_Identity=34.1666666666667, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI166795305, Length=218, Percent_Identity=31.651376146789, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI2367172, Length=355, Percent_Identity=43.6619718309859, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=40.7407407407407, Blast_Score=169, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=277, Percent_Identity=28.158844765343, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=34.5276872964169, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI19921226, Length=232, Percent_Identity=32.7586206896552, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_MYCA9 (B1MEU8)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001704208.1
- ProteinModelPortal:   B1MEU8
- SMR:   B1MEU8
- EnsemblBacteria:   EBMYCT00000008125
- GeneID:   5965978
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3478c
- GeneTree:   EBGT00050000015865
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   MABS561007:MAB_3478C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41088; Mature: 41088

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDCQADIADLDTTLTTVERVLDVDGLRARIKTLEEAAADPNLWDDQARGQQVTSQLSH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AQGELRRVEELRSRLDDLPVLYELAEESEDNSVVAEADAERVKLREDIEAMEVRTLLSGE
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
YDEREAVVTIRSGAGGVDAADWAEMLMRMYIRWAEAHKYPVEVFDTSYAEEAGIKSATFA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEE
VHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFADVEVLPVVETTDHIDIPEGDLRVD
EECCCEECEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEE
VYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKVAAMRVLQARLLERKRQE
EEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAEMDALKGDGGSSWGTQMRSYVLQPYQMVKDLRTEYEVGNPAAVLDGDIDGFIEAGIR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCC
WRNRRDD
CCCCCCC
>Mature Secondary Structure
MDLDCQADIADLDTTLTTVERVLDVDGLRARIKTLEEAAADPNLWDDQARGQQVTSQLSH
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
AQGELRRVEELRSRLDDLPVLYELAEESEDNSVVAEADAERVKLREDIEAMEVRTLLSGE
HHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCC
YDEREAVVTIRSGAGGVDAADWAEMLMRMYIRWAEAHKYPVEVFDTSYAEEAGIKSATFA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHCCCCCEEEE
VHAPFAYGTLSVEQGTHRLVRISPFDNQSRRQTSFADVEVLPVVETTDHIDIPEGDLRVD
EECCCEECEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCEEEE
VYRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQLQNKVAAMRVLQARLLERKRQE
EEECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ERAEMDALKGDGGSSWGTQMRSYVLQPYQMVKDLRTEYEVGNPAAVLDGDIDGFIEAGIR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCC
WRNRRDD
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA