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The map label for this gene is efpA [H]

Identifier: 169630224

GI number: 169630224

Start: 3180588

End: 3182105

Strand: Reverse

Name: efpA [H]

Synonym: MAB_3142c

Alternate gene names: 169630224

Gene position: 3182105-3180588 (Counterclockwise)

Preceding gene: 169630225

Following gene: 169630222

Centisome position: 62.8

GC content: 64.3

Gene sequence:

>1518_bases
ATGTCTGGCCTCAGCGTCGAGCAGCTCACTCAAAAGGCGCGCGAATTTCTGCCATCCCGGCACTACCTGTATGCCGTCAT
CGCGATCGCCGGTATGCAGTTCCTGGCCACCATGGACGGCACCATCGCGGTGGTCACGCTGCCCAAGATTCAGGCCGAGT
TGCACCTCAATGACGCCACCCGCAGCTGGGTGATCACCGCGTACATGTTGTCCTTCGGCGGGCTGATGCTGCTCGGCGGC
CGCCTGGGTGACACCTTCGGCCGCAAGCGGGTCTTCATCGGCGGCATTACGTTGTTCACCCTCGCCTCCATCGGGGTGGG
CCTCGCGCAAGAGGACATCGGTCTGGCGATTTTCCGCCTCATCCAGGGTGTGGGTGCGGCCGTCGCCTCGCCCACGGCGC
TGGCCCTGATTGCGACCACCTTTCCCAAGGGTCCGCTGCGTACTGCCGCCGTGGGAGTTTTCGGGGCCATGACGGGGGTG
GGCTCGATCGCCGGGCTGATTGTCGGCGGTGCACTGGCCGAGGTGTCCTGGCGCCTGGCCTTCCTCATCAACGTGCCGGC
GGGTGCCCTGATGATCTATCTGGCCGTGCGGTCACTGACCGAGACCGAACGCGAGCCGATGAAGCTGGATGTGGCGGGTT
CGGTGCTTGCCACGCTGGGCTGTACGGCGGCGGTGTTCGGATTCACCCAGGGGCCCGACCGCGGTTGGGATTCGCCGTAC
ACGATCGTGTCGCTGATCGCTGCCGCCGCGCTGCTGATCGCCTTCCTATTTGTGGAACGCACCGCAGAGAACCCGGTGTT
GCCGTTCAGTCTGTTTACCGATCGCAACCGGGTCGCGACATTTGCGGCGATCTTCATGGCGGGCGGGGTGCTCTTCACTC
TCACCATCACCATCGGCCTGTACATGCAGGATCTGATGAAGTACAGCCCGCTGCGGACTGGAATCGCGGCCATTCCTTTC
GTGGTCGGCATGGGTATCGGCCTGGGCTTATCGTCACAACTCGTCACGCGGCTGGCGCCGCGGGTGCTGATCCTGTGTGG
TGGCGTGGTCGTGTTCGGCGCCATGCTCTACGGCTCGACCATCGACCGGACCATCGCGTACTTCCCGGATTTCGCGACGG
CCATCTTCGTCGGTGGAATCGGTATCGGGACCATCGTGGTGCCGGTTATCTTGTCGGCGATCACCGGCGTCGATGCCGAC
CGCATCGGCCCGCTATCCGCCATTTCGCTCATGCTGCAAAACCTGGGCGGGCCAATCGTTCTGGTGATCATCCAAGCGAT
CATCACGTCGCGGACGCTCTATCTCGGTGGCGCCACGGGCCCGGTGCAGAACATGAACCGGGACCAGCTGCACGCCCTTG
ACCACGGCTACACCTACGGCATGCTGTGGATTGCCGGAACGGCCGTCCTGGTGGGTGTGGCGGCGTTGTTCATCACCTAC
AGCGCGGCCGACATCGCCCACGCTCAGAAGGCGCAGGCCGCGCACGACCAGGGTCTGGACGAGGACGAGCTCGAATAG

Upstream 100 bases:

>100_bases
GGGCATCAGACCGCCGGCAATCATCGTCATCGGGCCTGTCGCTGCTTACGGCGCCTTCTGATCTCTAAAGCGACGTTAAG
AGGACGGTAAGGTGTCCTCC

Downstream 100 bases:

>100_bases
GCCCTAGCAGTCCTGCGGGGTGTAATCGCCGGCGCGAAAGAGCAGTCCATTGGTGACGGGTATGCCGTCATTCACGGTGA
TGTGGCAGGACATTCGAAAG

Product: putative transmembrane efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux protein A [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSGLSVEQLTQKAREFLPSRHYLYAVIAIAGMQFLATMDGTIAVVTLPKIQAELHLNDATRSWVITAYMLSFGGLMLLGG
RLGDTFGRKRVFIGGITLFTLASIGVGLAQEDIGLAIFRLIQGVGAAVASPTALALIATTFPKGPLRTAAVGVFGAMTGV
GSIAGLIVGGALAEVSWRLAFLINVPAGALMIYLAVRSLTETEREPMKLDVAGSVLATLGCTAAVFGFTQGPDRGWDSPY
TIVSLIAAAALLIAFLFVERTAENPVLPFSLFTDRNRVATFAAIFMAGGVLFTLTITIGLYMQDLMKYSPLRTGIAAIPF
VVGMGIGLGLSSQLVTRLAPRVLILCGGVVVFGAMLYGSTIDRTIAYFPDFATAIFVGGIGIGTIVVPVILSAITGVDAD
RIGPLSAISLMLQNLGGPIVLVIIQAIITSRTLYLGGATGPVQNMNRDQLHALDHGYTYGMLWIAGTAVLVGVAALFITY
SAADIAHAQKAQAAHDQGLDEDELE

Sequences:

>Translated_505_residues
MSGLSVEQLTQKAREFLPSRHYLYAVIAIAGMQFLATMDGTIAVVTLPKIQAELHLNDATRSWVITAYMLSFGGLMLLGG
RLGDTFGRKRVFIGGITLFTLASIGVGLAQEDIGLAIFRLIQGVGAAVASPTALALIATTFPKGPLRTAAVGVFGAMTGV
GSIAGLIVGGALAEVSWRLAFLINVPAGALMIYLAVRSLTETEREPMKLDVAGSVLATLGCTAAVFGFTQGPDRGWDSPY
TIVSLIAAAALLIAFLFVERTAENPVLPFSLFTDRNRVATFAAIFMAGGVLFTLTITIGLYMQDLMKYSPLRTGIAAIPF
VVGMGIGLGLSSQLVTRLAPRVLILCGGVVVFGAMLYGSTIDRTIAYFPDFATAIFVGGIGIGTIVVPVILSAITGVDAD
RIGPLSAISLMLQNLGGPIVLVIIQAIITSRTLYLGGATGPVQNMNRDQLHALDHGYTYGMLWIAGTAVLVGVAALFITY
SAADIAHAQKAQAAHDQGLDEDELE
>Mature_504_residues
SGLSVEQLTQKAREFLPSRHYLYAVIAIAGMQFLATMDGTIAVVTLPKIQAELHLNDATRSWVITAYMLSFGGLMLLGGR
LGDTFGRKRVFIGGITLFTLASIGVGLAQEDIGLAIFRLIQGVGAAVASPTALALIATTFPKGPLRTAAVGVFGAMTGVG
SIAGLIVGGALAEVSWRLAFLINVPAGALMIYLAVRSLTETEREPMKLDVAGSVLATLGCTAAVFGFTQGPDRGWDSPYT
IVSLIAAAALLIAFLFVERTAENPVLPFSLFTDRNRVATFAAIFMAGGVLFTLTITIGLYMQDLMKYSPLRTGIAAIPFV
VGMGIGLGLSSQLVTRLAPRVLILCGGVVVFGAMLYGSTIDRTIAYFPDFATAIFVGGIGIGTIVVPVILSAITGVDADR
IGPLSAISLMLQNLGGPIVLVIIQAIITSRTLYLGGATGPVQNMNRDQLHALDHGYTYGMLWIAGTAVLVGVAALFITYS
AADIAHAQKAQAAHDQGLDEDELE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1788710, Length=440, Percent_Identity=27.5, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789042, Length=433, Percent_Identity=26.0969976905312, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87081983, Length=339, Percent_Identity=27.7286135693215, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790195, Length=329, Percent_Identity=27.9635258358663, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788392, Length=313, Percent_Identity=24.6006389776358, Blast_Score=63, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323935, Length=333, Percent_Identity=29.1291291291291, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324954, Length=272, Percent_Identity=29.4117647058824, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323520, Length=298, Percent_Identity=28.1879194630872, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323735, Length=340, Percent_Identity=26.1764705882353, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52916; Mature: 52785

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLSVEQLTQKAREFLPSRHYLYAVIAIAGMQFLATMDGTIAVVTLPKIQAELHLNDAT
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCH
RSWVITAYMLSFGGLMLLGGRLGDTFGRKRVFIGGITLFTLASIGVGLAQEDIGLAIFRL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHH
IQGVGAAVASPTALALIATTFPKGPLRTAAVGVFGAMTGVGSIAGLIVGGALAEVSWRLA
HHHHHHHHCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
FLINVPAGALMIYLAVRSLTETEREPMKLDVAGSVLATLGCTAAVFGFTQGPDRGWDSPY
EEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
TIVSLIAAAALLIAFLFVERTAENPVLPFSLFTDRNRVATFAAIFMAGGVLFTLTITIGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHH
YMQDLMKYSPLRTGIAAIPFVVGMGIGLGLSSQLVTRLAPRVLILCGGVVVFGAMLYGST
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
IDRTIAYFPDFATAIFVGGIGIGTIVVPVILSAITGVDADRIGPLSAISLMLQNLGGPIV
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHCCCHHH
LVIIQAIITSRTLYLGGATGPVQNMNRDQLHALDHGYTYGMLWIAGTAVLVGVAALFITY
HHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SAADIAHAQKAQAAHDQGLDEDELE
HHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure 
SGLSVEQLTQKAREFLPSRHYLYAVIAIAGMQFLATMDGTIAVVTLPKIQAELHLNDAT
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCH
RSWVITAYMLSFGGLMLLGGRLGDTFGRKRVFIGGITLFTLASIGVGLAQEDIGLAIFRL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHHHHH
IQGVGAAVASPTALALIATTFPKGPLRTAAVGVFGAMTGVGSIAGLIVGGALAEVSWRLA
HHHHHHHHCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
FLINVPAGALMIYLAVRSLTETEREPMKLDVAGSVLATLGCTAAVFGFTQGPDRGWDSPY
EEEECCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
TIVSLIAAAALLIAFLFVERTAENPVLPFSLFTDRNRVATFAAIFMAGGVLFTLTITIGL
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHH
YMQDLMKYSPLRTGIAAIPFVVGMGIGLGLSSQLVTRLAPRVLILCGGVVVFGAMLYGST
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
IDRTIAYFPDFATAIFVGGIGIGTIVVPVILSAITGVDADRIGPLSAISLMLQNLGGPIV
HHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHCCCHHH
LVIIQAIITSRTLYLGGATGPVQNMNRDQLHALDHGYTYGMLWIAGTAVLVGVAALFITY
HHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SAADIAHAQKAQAAHDQGLDEDELE
HHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]