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The map label for this gene is proS

Identifier: 169630222

GI number: 169630222

Start: 3178367

End: 3180109

Strand: Reverse

Name: proS

Synonym: MAB_3140c

Alternate gene names: 169630222

Gene position: 3180109-3178367 (Counterclockwise)

Preceding gene: 169630224

Following gene: 169630221

Centisome position: 62.76

GC content: 65.46

Gene sequence:

>1743_bases
ATGATTACCCGGCTCTCCGAGCTTTTCGTACGTACGCTGCGCGACGATCCGGCCGACGCCGAGGTTCCCAGCCACAAGCT
GTTGATCCGCGCGGGATACGTGCGCCCCGTCGCACCCGGCGTGTACAGCTGGCTGCCGCTCGGGCTGCGGGTGCTGCGCA
AGATCGAGAACATCGTTCGCGAAGAGATGAATGCCATTGGTGGCCAAGAGATTCTGCTGCCTGCCCTGCTGCCGCGCGCG
CCCTACGAGACCACGAACCGGTGGACCGAGTACGGGGATTCGCTGTTCCGGCTCAAGGACCGCCGCGACAACGACATGAT
GTTGGGCCCAACCCACGAGGAGCTGTTCGCGCTCACGGTCAAAGGGGAGTACAGCTCCTACAAGGATTTTCCGGTCATCC
TCTACCAAGTACAGACCAAGTACCGGGACGAGGCGCGGCCGCGGGCGGGCATCCTGCGTGGCCGAGAATTCGTGATGAAG
GACTCGTATTCCTTTGACACCTCCGATGACGGGCTCAAGGCGGCTTACCACGCCCACCGCGATGCCTACCAACGGATCTT
CGGTCGGCTCGGCCTGGACTACGTGATCGTGGCAGCCACCTCCGGTGCCATGGGTGGTAGCGCTTCTGAGGAATTCCTCG
CCGAAAGCCCCACCGGAGAAGACACTTTCGTGCGGTGCGTGGAGTCGGGGTACGCGGCCAACGTCGAGGCGGTTATCACG
CCCGCGCCGCCGGCCCGGCCCATCGAAGGACTGGCCGAGGCCGTCGTACACGAAACCGGTGATACCCCAACCATCGCCAC
CCTGGTGGATTGGGCCAACTCCGCCGGTCTCGGCAGAACCGTGACCGCCGCCGACACCCTCAAGAACATTCTGCTCAAGG
TGCGTCAGCCGGGCGGAGACTGGGAGCTGCTGGCCGTCGGCGTGCCCGGCGACCGTGAGGTCGACGACAAGCGTCTCGGA
GCGGCGCTCGAGCCCGCGGAGTATGAGCTGCTCGGCGATGCGGATTTTGCCAAGTATCCATTTTTGGTGCGCGGTTACAT
CGGGCCGAAGGCCTTGTTGGCCAACGGTGTCCGATACCTCGTCGATCCGCGGGTGGTGGAGGGCACCAGCTGGATCACCG
GCGCCGACGAGCCGGGCAAGCATGTGGTTGACCTCGTGGCCGGTCGCGACTTCACCGCGGACGGCACCATCGAGGCCGCC
GAGGTGCGTGACGGCGACCCCTCGCCCGACGGTGCTGGGCAGCTGGTGTCGGCCCGCGGCATCGAGATCGGCCATATCTT
CCAATTGGGCCGCAAGTACACGGATGCGTTCACTGTCGACGTGCTCGGTGAGAATGGCAAGCCGGTGCGGCTGACCCAGG
GCTCCTACGGTATCGGGGTGTCCCGGTTGGTGGCCGTGGTCGCCGAACAGCAGCATGACGAGCTGGGGCTGCGGTGGCCC
TCGGCGGTGTCACCATTCGACGTGCACGTGGTGATCGCCAACAAGGACGACGCCGCACGTGCGGGGGCCGAGGAGCTGGC
CGCCGAACTGGACCGGTTGGGCCACGAGGTGCTGCTCGATGATCGCACCGCCTCGCCAGGAGTGAAGTTCAAGGACGCCG
AGCTGCTGGGTGTGCCTTGGATTGTGGTGATCGGTCGGGGCTGGGCAGACGGAACCATCGAACTGCGCAACCGATTCACC
GGGGAGACGCAGCCCATCGCGGTGACCGACGCCGTGGCGTCCGTCACACAAGCGATAGGGTAA

Upstream 100 bases:

>100_bases
CGGAGGCCACGGCGGCACGCCGATATGCTGTGCAACCGTAACTGACGGTCTTCGCCCGAGAGGCTCAACGAAACATGGTC
TTCGCCCGAGAGGCTCATCA

Downstream 100 bases:

>100_bases
TGGTCATGCCGTCTTTCAAGCTCGAGCCGCTGGTCGAGAGTGATTTCAACACAAGCGATTTCGTGTATACGTTCTCCACG
CCGCTGCCCAAGGACGCGGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MITRLSELFVRTLRDDPADAEVPSHKLLIRAGYVRPVAPGVYSWLPLGLRVLRKIENIVREEMNAIGGQEILLPALLPRA
PYETTNRWTEYGDSLFRLKDRRDNDMMLGPTHEELFALTVKGEYSSYKDFPVILYQVQTKYRDEARPRAGILRGREFVMK
DSYSFDTSDDGLKAAYHAHRDAYQRIFGRLGLDYVIVAATSGAMGGSASEEFLAESPTGEDTFVRCVESGYAANVEAVIT
PAPPARPIEGLAEAVVHETGDTPTIATLVDWANSAGLGRTVTAADTLKNILLKVRQPGGDWELLAVGVPGDREVDDKRLG
AALEPAEYELLGDADFAKYPFLVRGYIGPKALLANGVRYLVDPRVVEGTSWITGADEPGKHVVDLVAGRDFTADGTIEAA
EVRDGDPSPDGAGQLVSARGIEIGHIFQLGRKYTDAFTVDVLGENGKPVRLTQGSYGIGVSRLVAVVAEQQHDELGLRWP
SAVSPFDVHVVIANKDDAARAGAEELAAELDRLGHEVLLDDRTASPGVKFKDAELLGVPWIVVIGRGWADGTIELRNRFT
GETQPIAVTDAVASVTQAIG

Sequences:

>Translated_580_residues
MITRLSELFVRTLRDDPADAEVPSHKLLIRAGYVRPVAPGVYSWLPLGLRVLRKIENIVREEMNAIGGQEILLPALLPRA
PYETTNRWTEYGDSLFRLKDRRDNDMMLGPTHEELFALTVKGEYSSYKDFPVILYQVQTKYRDEARPRAGILRGREFVMK
DSYSFDTSDDGLKAAYHAHRDAYQRIFGRLGLDYVIVAATSGAMGGSASEEFLAESPTGEDTFVRCVESGYAANVEAVIT
PAPPARPIEGLAEAVVHETGDTPTIATLVDWANSAGLGRTVTAADTLKNILLKVRQPGGDWELLAVGVPGDREVDDKRLG
AALEPAEYELLGDADFAKYPFLVRGYIGPKALLANGVRYLVDPRVVEGTSWITGADEPGKHVVDLVAGRDFTADGTIEAA
EVRDGDPSPDGAGQLVSARGIEIGHIFQLGRKYTDAFTVDVLGENGKPVRLTQGSYGIGVSRLVAVVAEQQHDELGLRWP
SAVSPFDVHVVIANKDDAARAGAEELAAELDRLGHEVLLDDRTASPGVKFKDAELLGVPWIVVIGRGWADGTIELRNRFT
GETQPIAVTDAVASVTQAIG
>Mature_580_residues
MITRLSELFVRTLRDDPADAEVPSHKLLIRAGYVRPVAPGVYSWLPLGLRVLRKIENIVREEMNAIGGQEILLPALLPRA
PYETTNRWTEYGDSLFRLKDRRDNDMMLGPTHEELFALTVKGEYSSYKDFPVILYQVQTKYRDEARPRAGILRGREFVMK
DSYSFDTSDDGLKAAYHAHRDAYQRIFGRLGLDYVIVAATSGAMGGSASEEFLAESPTGEDTFVRCVESGYAANVEAVIT
PAPPARPIEGLAEAVVHETGDTPTIATLVDWANSAGLGRTVTAADTLKNILLKVRQPGGDWELLAVGVPGDREVDDKRLG
AALEPAEYELLGDADFAKYPFLVRGYIGPKALLANGVRYLVDPRVVEGTSWITGADEPGKHVVDLVAGRDFTADGTIEAA
EVRDGDPSPDGAGQLVSARGIEIGHIFQLGRKYTDAFTVDVLGENGKPVRLTQGSYGIGVSRLVAVVAEQQHDELGLRWP
SAVSPFDVHVVIANKDDAARAGAEELAAELDRLGHEVLLDDRTASPGVKFKDAELLGVPWIVVIGRGWADGTIELRNRFT
GETQPIAVTDAVASVTQAIG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=42.1276595744681, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1786392, Length=585, Percent_Identity=41.7094017094017, Blast_Score=422, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI115532348, Length=237, Percent_Identity=37.1308016877637, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320931, Length=573, Percent_Identity=28.7958115183246, Blast_Score=232, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656200, Length=220, Percent_Identity=37.2727272727273, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_MYCA9 (B1MD96)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703871.1
- ProteinModelPortal:   B1MD96
- SMR:   B1MD96
- EnsemblBacteria:   EBMYCT00000006558
- GeneID:   5965645
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_3140c
- GeneTree:   EBGT00050000016802
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- BioCyc:   MABS561007:MAB_3140C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 62999; Mature: 62999

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLSELFVRTLRDDPADAEVPSHKLLIRAGYVRPVAPGVYSWLPLGLRVLRKIENIVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEMNAIGGQEILLPALLPRAPYETTNRWTEYGDSLFRLKDRRDNDMMLGPTHEELFALTV
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCHHEEECCCCCCCEEECCCHHHEEEEEE
KGEYSSYKDFPVILYQVQTKYRDEARPRAGILRGREFVMKDSYSFDTSDDGLKAAYHAHR
ECCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHH
DAYQRIFGRLGLDYVIVAATSGAMGGSASEEFLAESPTGEDTFVRCVESGYAANVEAVIT
HHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEE
PAPPARPIEGLAEAVVHETGDTPTIATLVDWANSAGLGRTVTAADTLKNILLKVRQPGGD
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCC
WELLAVGVPGDREVDDKRLGAALEPAEYELLGDADFAKYPFLVRGYIGPKALLANGVRYL
EEEEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCHHCCEEEEECCCHHHHHHCCCEEE
VDPRVVEGTSWITGADEPGKHVVDLVAGRDFTADGTIEAAEVRDGDPSPDGAGQLVSARG
ECCEEECCCCEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCC
IEIGHIFQLGRKYTDAFTVDVLGENGKPVRLTQGSYGIGVSRLVAVVAEQQHDELGLRWP
CCHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
SAVSPFDVHVVIANKDDAARAGAEELAAELDRLGHEVLLDDRTASPGVKFKDAELLGVPW
CCCCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEECCCEEECCCE
IVVIGRGWADGTIELRNRFTGETQPIAVTDAVASVTQAIG
EEEEECCCCCCEEEEHHCCCCCCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MITRLSELFVRTLRDDPADAEVPSHKLLIRAGYVRPVAPGVYSWLPLGLRVLRKIENIVR
CCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EEMNAIGGQEILLPALLPRAPYETTNRWTEYGDSLFRLKDRRDNDMMLGPTHEELFALTV
HHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHCCHHEEECCCCCCCEEECCCHHHEEEEEE
KGEYSSYKDFPVILYQVQTKYRDEARPRAGILRGREFVMKDSYSFDTSDDGLKAAYHAHR
ECCCCCCCCCCEEEEEEHHHHHHHCCCCHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHH
DAYQRIFGRLGLDYVIVAATSGAMGGSASEEFLAESPTGEDTFVRCVESGYAANVEAVIT
HHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEE
PAPPARPIEGLAEAVVHETGDTPTIATLVDWANSAGLGRTVTAADTLKNILLKVRQPGGD
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCC
WELLAVGVPGDREVDDKRLGAALEPAEYELLGDADFAKYPFLVRGYIGPKALLANGVRYL
EEEEEEECCCCCCCCHHHCCCCCCCCCEEEECCCCCHHCCEEEEECCCHHHHHHCCCEEE
VDPRVVEGTSWITGADEPGKHVVDLVAGRDFTADGTIEAAEVRDGDPSPDGAGQLVSARG
ECCEEECCCCEECCCCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCC
IEIGHIFQLGRKYTDAFTVDVLGENGKPVRLTQGSYGIGVSRLVAVVAEQQHDELGLRWP
CCHHHHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
SAVSPFDVHVVIANKDDAARAGAEELAAELDRLGHEVLLDDRTASPGVKFKDAELLGVPW
CCCCCEEEEEEEECCCCHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCEECCCEEECCCE
IVVIGRGWADGTIELRNRFTGETQPIAVTDAVASVTQAIG
EEEEECCCCCCEEEEHHCCCCCCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA