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The map label for this gene is gcvH

Identifier: 169629488

GI number: 169629488

Start: 2458219

End: 2458614

Strand: Direct

Name: gcvH

Synonym: MAB_2402

Alternate gene names: 169629488

Gene position: 2458219-2458614 (Clockwise)

Preceding gene: 169629487

Following gene: 169629489

Centisome position: 48.51

GC content: 60.61

Gene sequence:

>396_bases
GTGACCGAAATTCCCGCCGATCTGCACTACACCGAGGAACACGAATGGGTGCGGCGCACCGGTGACACCACCGTGCGCAT
CGGAATCACCGACTACGCACAGTCGCAGCTCGGTGACGTGGTGTTTGTCCAGCTGCCGGATGTCGGCTCCGAGCTGACCG
CGGGCTCCACGTTCGGTGAGGTGGAATCCACCAAATCGGTATCGGACCTGTTCGCGCCCATCACGGCGAAAGTCATTGCC
GCGAATGGTGATTTGGATTCCGACCCACAGCTGGTCAATTCCGATCCCTACGGCGAGGGCTGGTTGGTTGATTTGGAAGC
CGCCAGTGCGGCCGACCTGGACGCGGCTCTTAATGACCTCTTAGACGCATCGGGATATGGTGACGCCACCGACTAG

Upstream 100 bases:

>100_bases
TTGCGAGAACCGAAACCTCGCCAATACGCTCAGCCCGGCAAGTAGCCCTGTGAGCCCAGCGGCTACACCGACCACTGACC
GAGAGCAAGGACGACTCACC

Downstream 100 bases:

>100_bases
GAGGACACCGGTTCTCCAACCAGTCCGGCGGCCATAATTTCGGGCAGACCACTGGCGGATACGGCAGAGGTGGCCCGTTA
CGGGCAGTCAGACGGTACGG

Product: glycine cleavage system H protein (GcvH)

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MTEIPADLHYTEEHEWVRRTGDTTVRIGITDYAQSQLGDVVFVQLPDVGSELTAGSTFGEVESTKSVSDLFAPITAKVIA
ANGDLDSDPQLVNSDPYGEGWLVDLEAASAADLDAALNDLLDASGYGDATD

Sequences:

>Translated_131_residues
MTEIPADLHYTEEHEWVRRTGDTTVRIGITDYAQSQLGDVVFVQLPDVGSELTAGSTFGEVESTKSVSDLFAPITAKVIA
ANGDLDSDPQLVNSDPYGEGWLVDLEAASAADLDAALNDLLDASGYGDATD
>Mature_130_residues
TEIPADLHYTEEHEWVRRTGDTTVRIGITDYAQSQLGDVVFVQLPDVGSELTAGSTFGEVESTKSVSDLFAPITAKVIAA
NGDLDSDPQLVNSDPYGEGWLVDLEAASAADLDAALNDLLDASGYGDATD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=111, Percent_Identity=37.8378378378378, Blast_Score=92, Evalue=9e-20,
Organism=Homo sapiens, GI89057342, Length=111, Percent_Identity=36.9369369369369, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1789271, Length=126, Percent_Identity=43.6507936507937, Blast_Score=124, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=111, Percent_Identity=46.8468468468468, Blast_Score=105, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=40.3361344537815, Blast_Score=102, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=110, Percent_Identity=39.0909090909091, Blast_Score=101, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=37.9310344827586, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_MYCA9 (B1MB62)

Other databases:

- EMBL:   CU458896
- RefSeq:   YP_001703137.1
- ProteinModelPortal:   B1MB62
- SMR:   B1MB62
- EnsemblBacteria:   EBMYCT00000008109
- GeneID:   5964912
- GenomeReviews:   CU458896_GR
- KEGG:   mab:MAB_2402
- GeneTree:   EBGT00050000015839
- HOGENOM:   HBG693789
- OMA:   THEWVRP
- BioCyc:   MABS561007:MAB_2402-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13878; Mature: 13747

Theoretical pI: Translated: 3.57; Mature: 3.57

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIPADLHYTEEHEWVRRTGDTTVRIGITDYAQSQLGDVVFVQLPDVGSELTAGSTFGE
CCCCCCCCEECCCHHHHHHCCCCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
VESTKSVSDLFAPITAKVIAANGDLDSDPQLVNSDPYGEGWLVDLEAASAADLDAALNDL
CCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LDASGYGDATD
HHCCCCCCCCC
>Mature Secondary Structure 
TEIPADLHYTEEHEWVRRTGDTTVRIGITDYAQSQLGDVVFVQLPDVGSELTAGSTFGE
CCCCCCCEECCCHHHHHHCCCCEEEEEECHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
VESTKSVSDLFAPITAKVIAANGDLDSDPQLVNSDPYGEGWLVDLEAASAADLDAALNDL
CCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHH
LDASGYGDATD
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA